Rh4AG253100

Belongs to the endosulfine family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011376
Physical Location & Seq
Reverse (-)
56773428 .. 56776185
2758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG253100.1

Sequence Viewer

Length: 477 bp
ATGTACGTAAATATCTCCCCCAAAACCAAAATACAAATATATTCTTATCGCACCCCCTCACTCAACCCCAATTCTTCTTCTTCGTCCATCCTTTTTCTTTGGATCCTTTTTTTTTACCAACTTGCCCTAGATTTTCTGTTGCATCTCCCACTTTGCTCTTCCACGAGGAGAGCTAAGATGTCAGATTGCAACAGAGGCCAAGACTATTTCTCTGTTCAACAGCATGAGTCACCAACAGCTGCTGACAAGTATGGAGGACTAGCACCAAAGAAGAAGCCTTTGATTTCAAAGGATCATGAACGTGCCTACTTTGATTCAGCAGATTGGGCACTATGCAAGCAAGGTGCTGGGGTGAGTCCAAAATCAACAGCAGCAGTGGAGACCTTACAACCAAAACTACAGAGAACGCCACACCAGCAGCTTCCTCCAAGGAGACCTGCTTGTACTACATCTGGTCATGATAGCCATGGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

158

Amino Acids

17.65

Weight (kDa)

9.46

Isoelectric Point (pI)

66.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endosulfine PF04667 73 - 148 5e-25 cAMP-regulated phosphoprotein/endosulfine conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16146
fragaria_vesca FvH4_4g19340 FvH4_4g19340
malus_domestica MD13G1157500.v1.1 MD16G1158400.v1.1
prunus_persica Prupe.1G123200_v2.0.a1
pyrus_communis pycom13g13480 pycom16g13330
rosa_chinensis RchiOBHm_Chr4g0424361
rosa_laevigata RLG00000007480
rosa_multiflora Rmu_sc0005995.1_g000008
rosa_roxburghii Rroxscaffold_5G00366310
rosa_rugosa Rorug04G0196800 Rorug04G0196900 Rorug04G0197000
rosa_samantha Rh4AG253100 Rh4BG258500 Rh4CG269600 Rh4DG252900
rosa_wichuraiana Rw4G021830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 445
AclWI GGATC 3 cut(s) 97, 110, 300
AfaI GTAC 2 cut(s) 5, 445
AgsI TTSAA 2 cut(s) 218, 288
AluBI AGCT 3 cut(s) 173, 239, 421
AluI AGCT 3 cut(s) 173, 239, 421
Alw26I GTCTC 2 cut(s) 374, 427
AlwI GGATC 3 cut(s) 97, 110, 300
AlwNI CAGNNNCTG 1 cut(s) 242
AoxI GGCC 1 cut(s) 196
ApeKI GCWGC 3 cut(s) 239, 371, 418
AsuHPI GGTGA 2 cut(s) 222, 364
BaeGI GKGCMC 1 cut(s) 331
BamHI GGATCC 1 cut(s) 102
BauI CACGAG 1 cut(s) 163
BbvI GCAGC 3 cut(s) 226, 383, 430
BccI CCATC 1 cut(s) 95
BcoDI GTCTC 2 cut(s) 374, 427
BfaI CTAG 2 cut(s) 128, 260
BfmI CTRYAG 1 cut(s) 398
BfuAI ACCTGC 1 cut(s) 445
BisI GCNGC 3 cut(s) 240, 372, 419
BlsI GCNGC 3 cut(s) 241, 373, 420
BmiI GGNNCC 1 cut(s) 104
BmsI GCATC 1 cut(s) 151
BsaAI YACGTR 1 cut(s) 7
BsaI GGTCTC 2 cut(s) 374, 427
BsaJI CCNNGG 2 cut(s) 428, 466
BseDI CCNNGG 2 cut(s) 428, 466
BseGI GGATG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 181
BseSI GKGCMC 1 cut(s) 331
BseXI GCAGC 3 cut(s) 226, 383, 430
BseYI CCCAGC 1 cut(s) 347
BshFI GGCC 1 cut(s) 198
BsmAI GTCTC 2 cut(s) 374, 427
BsnI GGCC 1 cut(s) 198
Bso31I GGTCTC 2 cut(s) 374, 427
Bsp1286I GDGCHC 1 cut(s) 331
Bsp143I GATC 2 cut(s) 102, 292
Bsp19I CCATGG 1 cut(s) 466
BspANI GGCC 1 cut(s) 198
BspHI TCATGA 2 cut(s) 295, 457
BspLI GGNNCC 1 cut(s) 104
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 3 cut(s) 97, 110, 300
BspQI GCTCTTC 1 cut(s) 163
BspTNI GGTCTC 2 cut(s) 374, 427
BssECI CCNNGG 2 cut(s) 428, 466
BssMI GATC 2 cut(s) 102, 292
BssSI CACGAG 1 cut(s) 163
BssT1I CCWWGG 2 cut(s) 428, 466
Bst2BI CACGAG 1 cut(s) 163
Bst6I CTCTTC 1 cut(s) 163
BstBAI YACGTR 1 cut(s) 7
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 1 cut(s) 174
BstDSI CCRYGG 1 cut(s) 466
BstF5I GGATG 1 cut(s) 87
BstKTI GATC 2 cut(s) 105, 295
BstMAI GTCTC 2 cut(s) 374, 427
BstMBI GATC 2 cut(s) 102, 292
BstMWI GCNNNNNNNGC 3 cut(s) 195, 326, 415
BstSFI CTRYAG 1 cut(s) 398
BstSLI GKGCMC 1 cut(s) 331
BstSNI TACGTA 1 cut(s) 7
BstV1I GCAGC 3 cut(s) 226, 383, 430
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 466
BtsCI GGATG 1 cut(s) 87
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 1 cut(s) 381
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 1 cut(s) 338
CaiI CAGNNNCTG 1 cut(s) 242
CciI TCATGA 2 cut(s) 295, 457
Csp6I GTAC 2 cut(s) 4, 444
CviAII CATG 4 cut(s) 224, 296, 458, 467
CviJI RGCY 6 cut(s) 173, 198, 239, 277, 421, 465
CviKI_1 RGCY 6 cut(s) 173, 198, 239, 277, 421, 465
CviQI GTAC 2 cut(s) 4, 444
DdeI CTNAG 1 cut(s) 174
DpnI GATC 2 cut(s) 104, 294
DpnII GATC 2 cut(s) 102, 292
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco105I TACGTA 1 cut(s) 7
Eco130I CCWWGG 2 cut(s) 428, 466
Eco31I GGTCTC 2 cut(s) 374, 427
EcoT14I CCWWGG 2 cut(s) 428, 466
ErhI CCWWGG 2 cut(s) 428, 466
FaeI CATG 4 cut(s) 227, 299, 461, 470
FaiI YATR 8 cut(s) 40, 225, 252, 297, 334, 459, 468, 475
FatI CATG 4 cut(s) 223, 295, 457, 466
Fnu4HI GCNGC 3 cut(s) 240, 372, 419
FokI GGATG 1 cut(s) 74
Fsp4HI GCNGC 3 cut(s) 240, 372, 419
FspBI CTAG 2 cut(s) 128, 260
GluI GCNGC 3 cut(s) 240, 372, 419
GsaI CCCAGC 1 cut(s) 351
HaeIII GGCC 1 cut(s) 198
Hin1II CATG 4 cut(s) 227, 299, 461, 470
HinfI GANTC 3 cut(s) 227, 314, 355
HphI GGTGA 2 cut(s) 222, 364
Hpy188I TCNGA 1 cut(s) 184
Hpy188III TCNNGA 2 cut(s) 296, 458
HpyCH4IV ACGT 2 cut(s) 6, 301
HpyCH4V TGCA 3 cut(s) 142, 189, 336
HpyF10VI GCNNNNNNNGC 3 cut(s) 195, 326, 415
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 2 cut(s) 6, 301
Hsp92II CATG 4 cut(s) 227, 299, 461, 470
Kzo9I GATC 2 cut(s) 102, 292
LguI GCTCTTC 1 cut(s) 163
LpnPI CCDG 4 cut(s) 333, 428, 438, 450
Lsp1109I GCAGC 3 cut(s) 226, 383, 430
LweI GCATC 1 cut(s) 151
MaeI CTAG 2 cut(s) 128, 260
MaeII ACGT 2 cut(s) 6, 301
MaeIII GTNAC 1 cut(s) 228
MalI GATC 2 cut(s) 104, 294
MboI GATC 2 cut(s) 102, 292
MboII GAAGA 5 cut(s) 66, 69, 72, 150, 283
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 1 cut(s) 331
MluCI AATT 1 cut(s) 70
MlyI GAGTC 2 cut(s) 236, 364
MnlI CCTC 5 cut(s) 67, 159, 188, 248, 435
MslI CAYNNNNRTG 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 239
MwoI GCNNNNNNNGC 3 cut(s) 195, 326, 415
NcoI CCATGG 1 cut(s) 466
NdeII GATC 2 cut(s) 102, 292
NlaIII CATG 4 cut(s) 227, 299, 461, 470
NlaIV GGNNCC 1 cut(s) 104
NmuCI GTSAC 1 cut(s) 228
PagI TCATGA 2 cut(s) 295, 457
PciSI GCTCTTC 1 cut(s) 163
PfeI GAWTC 1 cut(s) 314
PkrI GCNGC 3 cut(s) 241, 373, 420
PleI GAGTC 2 cut(s) 235, 363
PpsI GAGTC 2 cut(s) 235, 363
Ppu21I YACGTR 1 cut(s) 7
PspFI CCCAGC 1 cut(s) 347
PspN4I GGNNCC 1 cut(s) 104
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 1 cut(s) 102
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 2 cut(s) 5, 445
RsaNI GTAC 2 cut(s) 4, 444
RseI CAYNNNNRTG 1 cut(s) 300
SapI GCTCTTC 1 cut(s) 163
SatI GCNGC 3 cut(s) 240, 372, 419
Sau3AI GATC 2 cut(s) 102, 292
SchI GAGTC 2 cut(s) 236, 364
SduI GDGCHC 1 cut(s) 331
SetI ASST 8 cut(s) 9, 175, 241, 304, 346, 386, 423, 439
SfaNI GCATC 1 cut(s) 151
SfcI CTRYAG 1 cut(s) 398
SmiMI CAYNNNNRTG 1 cut(s) 300
SnaBI TACGTA 1 cut(s) 7
Sse9I AATT 1 cut(s) 70
SspMI CTAG 2 cut(s) 128, 260
StyI CCWWGG 2 cut(s) 428, 466
TaiI ACGT 2 cut(s) 9, 304
TasI AATT 1 cut(s) 70
TatI WGTACW 1 cut(s) 443
TfiI GAWTC 1 cut(s) 314
TscAI CASTG 1 cut(s) 381
TseFI GTSAC 1 cut(s) 228
TseI GCWGC 3 cut(s) 239, 371, 418
Tsp45I GTSAC 1 cut(s) 228
TspDTI ATGAA 1 cut(s) 312
TspRI CASTG 1 cut(s) 381
XspI CTAG 2 cut(s) 128, 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.