Rh4AG414600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0010560
Physical Location & Seq
Forward (+)
71352706 .. 71368382
15677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG414600.1

Sequence Viewer

Length: 375 bp
ATGGCTTCTCAGATCACATCGGCGATGTTACTTGCGGCGTGTTTCGCCGTCCTGATCATGGTTTGTGCAGCTGTTGGAGAAGGCCAGCGGCCCGTACCTCGAAACATGCAAGGAGTGAGGCCGGTACATGAAATAAAAGCCTTGAAAGGGTTGAAAGTCACAGAAGGCATGAGGCCAATACCAAAAGTCGTGACACTGATGGCGCCGAAGCTCGACATGCCCCCAATGCCTGGTACCACCCCCGCGCTCGGCATGCGGCCAATACATAAAAACATGAAAGTCACAAAAGGCATGAGGCCAATACCAAAAGTCGTGACAGAGAGGGCGGCGCCGAAGCTCGACATGCCCCCAATGCCCGGACAGGGGATGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

13.26

Weight (kDa)

10.55

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 3 cut(s) 202, 233, 328
AccB7I CCANNNNNTGG 1 cut(s) 230
AccII CGCG 1 cut(s) 245
AciI CCGC 5 cut(s) 35, 88, 243, 256, 326
AcoI YGGCCR 1 cut(s) 257
AcyI GRCGYC 2 cut(s) 203, 329
AfaI GTAC 3 cut(s) 96, 126, 235
AfiI CCNNNNNNNGG 7 cut(s) 58, 147, 230, 248, 356, 362, 363
AgsI TTSAA 2 cut(s) 145, 154
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 3 cut(s) 71, 211, 337
AluI AGCT 3 cut(s) 71, 211, 337
AoxI GGCC 6 cut(s) 82, 89, 119, 173, 257, 296
ApeKI GCWGC 1 cut(s) 68
Asp718I GGTACC 1 cut(s) 233
AspLEI GCGC 3 cut(s) 205, 247, 331
AspS9I GGNCC 1 cut(s) 90
AsuC2I CCSGG 1 cut(s) 357
BanI GGYRCC 3 cut(s) 202, 233, 328
BbvI GCAGC 1 cut(s) 80
BccI CCATC 2 cut(s) 193, 361
BceAI ACGGC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 231
BclI TGATCA 1 cut(s) 54
BcnI CCSGG 1 cut(s) 357
BfoI RGCGCY 2 cut(s) 206, 332
BisI GCNGC 5 cut(s) 36, 69, 89, 257, 327
BlsI GCNGC 5 cut(s) 37, 70, 90, 258, 328
Bme1390I CCNGG 2 cut(s) 231, 357
BmgT120I GGNCC 1 cut(s) 90
BmiI GGNNCC 3 cut(s) 204, 235, 330
BmrFI CCNGG 2 cut(s) 231, 357
BpuMI CCSGG 1 cut(s) 357
BsaHI GRCGYC 2 cut(s) 203, 329
Bsc4I CCNNNNNNNGG 7 cut(s) 58, 147, 230, 248, 356, 362, 363
Bse118I RCCGGY 1 cut(s) 121
BseBI CCWGG 1 cut(s) 231
BseGI GGATG 1 cut(s) 372
BseLI CCNNNNNNNGG 7 cut(s) 58, 147, 230, 248, 356, 362, 363
BseMII CTCAG 1 cut(s) 23
BseXI GCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 87
Bsh1236I CGCG 1 cut(s) 245
BshFI GGCC 6 cut(s) 84, 91, 121, 175, 259, 298
BshNI GGYRCC 3 cut(s) 202, 233, 328
BsiSI CCGG 2 cut(s) 122, 357
BslI CCNNNNNNNGG 7 cut(s) 58, 147, 230, 248, 356, 362, 363
BsnI GGCC 6 cut(s) 84, 91, 121, 175, 259, 298
Bsp143I GATC 2 cut(s) 12, 54
BspACI CCGC 5 cut(s) 35, 88, 243, 256, 326
BspANI GGCC 6 cut(s) 84, 91, 121, 175, 259, 298
BspCNI CTCAG 1 cut(s) 22
BspFNI CGCG 1 cut(s) 245
BspLI GGNNCC 3 cut(s) 204, 235, 330
BspT107I GGYRCC 3 cut(s) 202, 233, 328
BsrFI RCCGGY 1 cut(s) 121
BssAI RCCGGY 1 cut(s) 121
BssMI GATC 2 cut(s) 12, 54
BssNI GRCGYC 2 cut(s) 203, 329
Bst2UI CCWGG 1 cut(s) 231
BstACI GRCGYC 2 cut(s) 203, 329
BstC8I GCNNGC 2 cut(s) 86, 254
BstDEI CTNAG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 372
BstFNI CGCG 1 cut(s) 245
BstH2I RGCGCY 2 cut(s) 206, 332
BstHHI GCGC 3 cut(s) 205, 247, 331
BstKTI GATC 2 cut(s) 15, 57
BstMBI GATC 2 cut(s) 12, 54
BstMWI GCNNNNNNNGC 6 cut(s) 44, 217, 226, 253, 343, 352
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 4 cut(s) 109, 220, 256, 346
BstSCI CCNGG 2 cut(s) 229, 355
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 1 cut(s) 80
BsuRI GGCC 6 cut(s) 84, 91, 121, 175, 259, 298
BtgZI GCGATG 1 cut(s) 38
BtsCI GGATG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 86, 254
CfoI GCGC 3 cut(s) 205, 247, 331
Cfr10I RCCGGY 1 cut(s) 121
Cfr13I GGNCC 1 cut(s) 90
Csp6I GTAC 3 cut(s) 95, 125, 234
CviAII CATG 9 cut(s) 58, 106, 128, 169, 217, 253, 274, 292, 343
CviQI GTAC 3 cut(s) 95, 125, 234
DdeI CTNAG 1 cut(s) 9
DinI GGCGCC 2 cut(s) 204, 330
DpnI GATC 2 cut(s) 14, 56
DpnII GATC 2 cut(s) 12, 54
EaeI YGGCCR 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 229
EgeI GGCGCC 2 cut(s) 204, 330
EheI GGCGCC 2 cut(s) 204, 330
FaeI CATG 9 cut(s) 61, 109, 131, 172, 220, 256, 277, 295, 346
FatI CATG 9 cut(s) 57, 105, 127, 168, 216, 252, 273, 291, 342
FauI CCCGC 1 cut(s) 250
FbaI TGATCA 1 cut(s) 54
Fnu4HI GCNGC 5 cut(s) 36, 69, 89, 257, 327
Fsp4HI GCNGC 5 cut(s) 36, 69, 89, 257, 327
GlaI GCGC 3 cut(s) 204, 246, 330
GluI GCNGC 5 cut(s) 36, 69, 89, 257, 327
HaeII RGCGCY 2 cut(s) 206, 332
HaeIII GGCC 6 cut(s) 84, 91, 121, 175, 259, 298
HapII CCGG 2 cut(s) 122, 357
HhaI GCGC 3 cut(s) 205, 247, 331
Hin1I GRCGYC 2 cut(s) 203, 329
Hin1II CATG 9 cut(s) 61, 109, 131, 172, 220, 256, 277, 295, 346
Hin6I GCGC 3 cut(s) 203, 245, 329
HinP1I GCGC 3 cut(s) 203, 245, 329
HpaII CCGG 2 cut(s) 122, 357
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 3 cut(s) 52, 190, 313
HpyAV CCTTC 2 cut(s) 74, 158
HpyCH4V TGCA 2 cut(s) 68, 109
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 217, 226, 253, 343, 352
HpyF3I CTNAG 1 cut(s) 9
Hsp92I GRCGYC 2 cut(s) 203, 329
Hsp92II CATG 9 cut(s) 61, 109, 131, 172, 220, 256, 277, 295, 346
HspAI GCGC 3 cut(s) 203, 245, 329
KasI GGCGCC 2 cut(s) 202, 328
KpnI GGTACC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 2 cut(s) 12, 54
LpnPI CCDG 7 cut(s) 65, 98, 135, 216, 243, 347, 370
Lsp1109I GCAGC 1 cut(s) 80
MaeIII GTNAC 5 cut(s) 27, 157, 190, 280, 313
MalI GATC 2 cut(s) 14, 56
MboI GATC 2 cut(s) 12, 54
Mly113I GGCGCC 2 cut(s) 203, 329
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 5 cut(s) 108, 111, 165, 288, 315
MspA1I CMGCKG 2 cut(s) 71, 88
MspI CCGG 2 cut(s) 122, 357
MspR9I CCNGG 2 cut(s) 231, 357
MvaI CCWGG 1 cut(s) 231
MvnI CGCG 1 cut(s) 245
MwoI GCNNNNNNNGC 6 cut(s) 44, 217, 226, 253, 343, 352
NarI GGCGCC 2 cut(s) 203, 329
NciI CCSGG 1 cut(s) 357
NdeII GATC 2 cut(s) 12, 54
NlaIII CATG 9 cut(s) 61, 109, 131, 172, 220, 256, 277, 295, 346
NlaIV GGNNCC 3 cut(s) 204, 235, 330
NmeAIII GCCGAG 1 cut(s) 228
NmuCI GTSAC 4 cut(s) 157, 190, 280, 313
NspI RCATGY 4 cut(s) 109, 220, 256, 346
PaeI GCATGC 1 cut(s) 256
PflMI CCANNNNNTGG 1 cut(s) 230
PkrI GCNGC 5 cut(s) 37, 70, 90, 258, 328
PluTI GGCGCC 2 cut(s) 206, 332
Psp6I CCWGG 1 cut(s) 229
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 3 cut(s) 204, 235, 330
PspPI GGNCC 1 cut(s) 90
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 3 cut(s) 96, 126, 235
RsaNI GTAC 3 cut(s) 95, 125, 234
SatI GCNGC 5 cut(s) 36, 69, 89, 257, 327
Sau3AI GATC 2 cut(s) 12, 54
Sau96I GGNCC 1 cut(s) 90
ScrFI CCNGG 2 cut(s) 231, 357
SetI ASST 4 cut(s) 73, 100, 213, 339
SfoI GGCGCC 2 cut(s) 204, 330
SphI GCATGC 1 cut(s) 256
SsiI CCGC 5 cut(s) 35, 88, 243, 256, 326
SspDI GGCGCC 2 cut(s) 202, 328
StyD4I CCNGG 2 cut(s) 229, 355
TaqI TCGA 3 cut(s) 100, 213, 339
TauI GCSGC 4 cut(s) 38, 91, 259, 329
TscAI CASTG 1 cut(s) 201
TseFI GTSAC 4 cut(s) 157, 190, 280, 313
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 4 cut(s) 157, 190, 280, 313
TspDTI ATGAA 2 cut(s) 144, 290
TspRI CASTG 1 cut(s) 201
Van91I CCANNNNNTGG 1 cut(s) 230
XceI RCATGY 4 cut(s) 109, 220, 256, 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.