Rh4BG156400

MFP1 attachment factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011624
Physical Location & Seq
Reverse (-)
28243371 .. 28244359
989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG156400.1

Sequence Viewer

Length: 621 bp
ATGGAAGTCCAGGATTCACAGGATCGGAAGCCGAGCTCCGACTCCATCTACAGCCTATTCTTGCCGACCATGGTCTTCCGCAACACCGCCGTGGACCTTCTGTTCAATTTCCTCACCACTTCCGACGAGGCCGAAGTCGAGGCTCGCCTCTTCGAGGAAGACGCCTTCGCCACCTCCAGAACGGTTATCGGAGCGATCTACTCCGACGAGATCAGCGATCGCGTGCTGGAGGTCCTCAAGGCTAAGAACACCGGCGCTTCTGCTGCTGAAAAGGGCGCCCTGTTGTTGCCGGACGTTGGCATGCGCAAGCCCTTCGTGGGCATTATTATCAAGAACCTCAGCAGTCCCTCCTCTCCCTGGAGGCACTACGGCACCTTCTCCACCGACGAGGCCAAAGCCCTGGCTCCCTTCATCGAGCGAGAGGCCTTCTTCGCCGCCAGGGAGAAGATCCGTGAGATCTACATCGAGGAGGTCTGCAAGCGCATGTTCGAGATCAAACACAAGAACGCCGCTGCTTCTGCTGCTGGAAACGTCGCGTCGGAGACCGCCGCCGGTGGTGTGCCTGAGGCAATTGAAAGTCTGCTAAAATCCATGGAATTAGCAGAATCCATGGATTACTAA

Protein Analysis

206

Amino Acids

22.69

Weight (kDa)

4.83

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WPP PF13943 101 - 156 4.3e-06 WPP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 305
AccB1I GGYRCC 2 cut(s) 275, 371
AccII CGCG 2 cut(s) 222, 536
AciI CCGC 6 cut(s) 79, 87, 435, 510, 546, 549
AclWI GGATC 2 cut(s) 30, 442
AcyI GRCGYC 2 cut(s) 162, 276
AfiI CCNNNNNNNGG 4 cut(s) 154, 296, 317, 357
AgsI TTSAA 2 cut(s) 106, 575
AjnI CCWGG 4 cut(s) 9, 356, 399, 437
AleI CACNNNNGTG 1 cut(s) 89
AloI GAACNNNNNNTCC 2 cut(s) 86, 118
AluBI AGCT 1 cut(s) 36
AluI AGCT 1 cut(s) 36
Alw21I GWGCWC 1 cut(s) 38
Alw26I GTCTC 1 cut(s) 536
AlwI GGATC 2 cut(s) 30, 442
AoxI GGCC 3 cut(s) 129, 390, 423
ApeKI GCWGC 3 cut(s) 263, 512, 521
AsiSI GCGATCGC 1 cut(s) 220
AspLEI GCGC 4 cut(s) 257, 278, 306, 483
AspS9I GGNCC 2 cut(s) 94, 232
AsuHPI GGTGA 1 cut(s) 106
AvaII GGWCC 2 cut(s) 94, 232
AxyI CCTNAGG 1 cut(s) 564
BanI GGYRCC 2 cut(s) 275, 371
BanII GRGCYC 1 cut(s) 38
BbsI GAAGAC 2 cut(s) 67, 165
Bbv12I GWGCWC 1 cut(s) 38
BbvCI CCTCAGC 1 cut(s) 338
BbvI GCAGC 3 cut(s) 250, 499, 508
BccI CCATC 1 cut(s) 53
BceAI ACGGC 2 cut(s) 74, 385
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 4 cut(s) 11, 358, 401, 439
BcoDI GTCTC 1 cut(s) 536
BfmI CTRYAG 1 cut(s) 49
BfoI RGCGCY 2 cut(s) 258, 279
BglII AGATCT 1 cut(s) 456
BisI GCNGC 6 cut(s) 264, 435, 510, 513, 522, 549
BlsI GCNGC 6 cut(s) 265, 436, 511, 514, 523, 550
Bme1390I CCNGG 4 cut(s) 11, 358, 401, 439
Bme18I GGWCC 2 cut(s) 94, 232
BmgT120I GGNCC 2 cut(s) 94, 232
BmiI GGNNCC 3 cut(s) 277, 373, 405
BmrFI CCNGG 4 cut(s) 11, 358, 401, 439
BoxI GACNNNNGTC 1 cut(s) 71
BpiI GAAGAC 2 cut(s) 67, 165
BpmI CTGGAG 3 cut(s) 160, 248, 379
Bpu10I CCTNAGC 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 221
BsaBI GATNNNNATC 1 cut(s) 461
BsaHI GRCGYC 2 cut(s) 162, 276
BsaI GGTCTC 1 cut(s) 536
BsaJI CCNNGG 7 cut(s) 69, 90, 356, 399, 438, 591, 609
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 296, 317, 357
Bse118I RCCGGY 2 cut(s) 251, 551
Bse21I CCTNAGG 1 cut(s) 564
Bse8I GATNNNNATC 1 cut(s) 461
BseBI CCWGG 4 cut(s) 11, 358, 401, 439
BseDI CCNNGG 7 cut(s) 69, 90, 356, 399, 438, 591, 609
BseJI GATNNNNATC 1 cut(s) 461
BseLI CCNNNNNNNGG 4 cut(s) 154, 296, 317, 357
BseMII CTCAG 2 cut(s) 352, 555
BseRI GAGGAG 2 cut(s) 340, 482
BseXI GCAGC 3 cut(s) 250, 499, 508
Bsh1236I CGCG 2 cut(s) 222, 536
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 3 cut(s) 131, 392, 425
BshNI GGYRCC 2 cut(s) 275, 371
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 38
BsiSI CCGG 3 cut(s) 252, 290, 552
BslFI GGGAC 1 cut(s) 330
BslI CCNNNNNNNGG 4 cut(s) 154, 296, 317, 357
BsmAI GTCTC 1 cut(s) 536
BsmFI GGGAC 1 cut(s) 330
BsnI GGCC 3 cut(s) 131, 392, 425
Bso31I GGTCTC 1 cut(s) 536
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 7 cut(s) 22, 195, 210, 217, 447, 456, 492
Bsp19I CCATGG 3 cut(s) 69, 591, 609
BspACI CCGC 6 cut(s) 79, 87, 435, 510, 546, 549
BspANI GGCC 3 cut(s) 131, 392, 425
BspCNI CTCAG 2 cut(s) 351, 556
BspFNI CGCG 2 cut(s) 222, 536
BspLI GGNNCC 3 cut(s) 277, 373, 405
BspPI GGATC 2 cut(s) 30, 442
BspT107I GGYRCC 2 cut(s) 275, 371
BspTNI GGTCTC 1 cut(s) 536
BsrFI RCCGGY 2 cut(s) 251, 551
BssAI RCCGGY 2 cut(s) 251, 551
BssECI CCNNGG 7 cut(s) 69, 90, 356, 399, 438, 591, 609
BssMI GATC 7 cut(s) 22, 195, 210, 217, 447, 456, 492
BssNI GRCGYC 2 cut(s) 162, 276
BssT1I CCWWGG 3 cut(s) 69, 591, 609
Bst2UI CCWGG 4 cut(s) 11, 358, 401, 439
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 2 cut(s) 162, 276
BstC8I GCNNGC 5 cut(s) 145, 224, 302, 308, 479
BstDEI CTNAG 3 cut(s) 243, 338, 564
BstDSI CCRYGG 4 cut(s) 69, 90, 591, 609
BstENI CCTNNNNNAGG 1 cut(s) 152
BstFNI CGCG 2 cut(s) 222, 536
BstH2I RGCGCY 2 cut(s) 258, 279
BstHHI GCGC 4 cut(s) 257, 278, 306, 483
BstKTI GATC 7 cut(s) 25, 198, 213, 220, 450, 459, 495
BstMAI GTCTC 1 cut(s) 536
BstMBI GATC 7 cut(s) 22, 195, 210, 217, 447, 456, 492
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 4 cut(s) 263, 431, 518, 521
BstNI CCWGG 4 cut(s) 11, 358, 401, 439
BstNSI RCATGY 2 cut(s) 304, 487
BstPAI GACNNNNGTC 1 cut(s) 71
BstSCI CCNGG 4 cut(s) 9, 356, 399, 437
BstSFI CTRYAG 1 cut(s) 49
BstUI CGCG 2 cut(s) 222, 536
BstV1I GCAGC 3 cut(s) 250, 499, 508
BstV2I GAAGAC 2 cut(s) 67, 165
BstX2I RGATCY 2 cut(s) 447, 456
BstXI CCANNNNNNTGG 1 cut(s) 400
BstYI RGATCY 2 cut(s) 447, 456
Bsu36I CCTNAGG 1 cut(s) 564
BsuRI GGCC 3 cut(s) 131, 392, 425
BtgI CCRYGG 4 cut(s) 69, 90, 591, 609
Cac8I GCNNGC 5 cut(s) 145, 224, 302, 308, 479
CfoI GCGC 4 cut(s) 257, 278, 306, 483
Cfr10I RCCGGY 2 cut(s) 251, 551
Cfr13I GGNCC 2 cut(s) 94, 232
CseI GACGC 2 cut(s) 170, 525
CviAII CATG 5 cut(s) 70, 301, 484, 592, 610
DdeI CTNAG 3 cut(s) 243, 338, 564
DinI GGCGCC 1 cut(s) 277
DpnI GATC 7 cut(s) 24, 197, 212, 219, 449, 458, 494
DpnII GATC 7 cut(s) 22, 195, 210, 217, 447, 456, 492
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
Ecl136II GAGCTC 1 cut(s) 36
Eco130I CCWWGG 3 cut(s) 69, 591, 609
Eco147I AGGCCT 1 cut(s) 425
Eco24I GRGCYC 1 cut(s) 38
Eco31I GGTCTC 1 cut(s) 536
Eco47I GGWCC 2 cut(s) 94, 232
Eco53kI GAGCTC 1 cut(s) 36
Eco81I CCTNAGG 1 cut(s) 564
EcoICRI GAGCTC 1 cut(s) 36
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 232
EcoRII CCWGG 4 cut(s) 9, 356, 399, 437
EcoT14I CCWWGG 3 cut(s) 69, 591, 609
EcoT38I GRGCYC 1 cut(s) 38
EgeI GGCGCC 1 cut(s) 277
EheI GGCGCC 1 cut(s) 277
ErhI CCWWGG 3 cut(s) 69, 591, 609
FaeI CATG 5 cut(s) 73, 304, 487, 595, 613
FaiI YATR 5 cut(s) 71, 302, 485, 593, 611
FaqI GGGAC 1 cut(s) 330
FatI CATG 5 cut(s) 69, 300, 483, 591, 609
Fnu4HI GCNGC 6 cut(s) 264, 435, 510, 513, 522, 549
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 6 cut(s) 264, 435, 510, 513, 522, 549
FspI TGCGCA 1 cut(s) 305
GlaI GCGC 4 cut(s) 256, 277, 305, 482
GluI GCNGC 6 cut(s) 264, 435, 510, 513, 522, 549
GsuI CTGGAG 3 cut(s) 160, 248, 379
HaeII RGCGCY 2 cut(s) 258, 279
HaeIII GGCC 3 cut(s) 131, 392, 425
HapII CCGG 3 cut(s) 252, 290, 552
HgaI GACGC 2 cut(s) 170, 525
HhaI GCGC 4 cut(s) 257, 278, 306, 483
Hin1I GRCGYC 2 cut(s) 162, 276
Hin1II CATG 5 cut(s) 73, 304, 487, 595, 613
Hin6I GCGC 4 cut(s) 255, 276, 304, 481
HinP1I GCGC 4 cut(s) 255, 276, 304, 481
HinfI GANTC 3 cut(s) 14, 41, 605
HpaII CCGG 3 cut(s) 252, 290, 552
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 6 cut(s) 27, 40, 124, 191, 205, 541
Hpy188III TCNNGA 3 cut(s) 177, 331, 490
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 5 cut(s) 128, 209, 389, 536, 541
HpyAV CCTTC 6 cut(s) 107, 175, 322, 385, 418, 436
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 2 cut(s) 294, 531
HpyCH4V TGCA 1 cut(s) 477
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 431, 518, 521
HpyF3I CTNAG 3 cut(s) 243, 338, 564
HpySE526I ACGT 2 cut(s) 294, 531
Hsp92I GRCGYC 2 cut(s) 162, 276
Hsp92II CATG 5 cut(s) 73, 304, 487, 595, 613
HspAI GCGC 4 cut(s) 255, 276, 304, 481
KasI GGCGCC 1 cut(s) 275
Kzo9I GATC 7 cut(s) 22, 195, 210, 217, 447, 456, 492
LmnI GCTCC 3 cut(s) 41, 191, 409
Lsp1109I GCAGC 3 cut(s) 250, 499, 508
MaeII ACGT 2 cut(s) 294, 531
MalI GATC 7 cut(s) 24, 197, 212, 219, 449, 458, 494
MboI GATC 7 cut(s) 22, 195, 210, 217, 447, 456, 492
MboII GAAGA 5 cut(s) 67, 142, 170, 421, 457
MfeI CAATTG 1 cut(s) 570
MflI RGATCY 2 cut(s) 447, 456
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 3 cut(s) 106, 570, 596
Mly113I GGCGCC 1 cut(s) 276
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 4 cut(s) 63, 147, 228, 519
MslI CAYNNNNRTG 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 3 cut(s) 252, 290, 552
MspR9I CCNGG 4 cut(s) 11, 358, 401, 439
MunI CAATTG 1 cut(s) 570
MvaI CCWGG 4 cut(s) 11, 358, 401, 439
MvnI CGCG 2 cut(s) 222, 536
MwoI GCNNNNNNNGC 4 cut(s) 263, 431, 518, 521
NarI GGCGCC 1 cut(s) 276
NcoI CCATGG 3 cut(s) 69, 591, 609
NdeII GATC 7 cut(s) 22, 195, 210, 217, 447, 456, 492
NlaIII CATG 5 cut(s) 73, 304, 487, 595, 613
NlaIV GGNNCC 3 cut(s) 277, 373, 405
NmeAIII GCCGAG 1 cut(s) 57
NsbI TGCGCA 1 cut(s) 305
NspI RCATGY 2 cut(s) 304, 487
OliI CACNNNNGTG 1 cut(s) 89
PaeI GCATGC 1 cut(s) 304
PceI AGGCCT 1 cut(s) 425
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PfeI GAWTC 2 cut(s) 14, 605
PfoI TCCNGGA 1 cut(s) 9
PkrI GCNGC 6 cut(s) 265, 436, 511, 514, 523, 550
Ple19I CGATCG 1 cut(s) 220
PleI GAGTC 1 cut(s) 35
PluTI GGCGCC 1 cut(s) 279
PpsI GAGTC 1 cut(s) 35
PpuMI RGGWCCY 1 cut(s) 232
PshAI GACNNNNGTC 1 cut(s) 71
Psp124BI GAGCTC 1 cut(s) 38
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 4 cut(s) 9, 356, 399, 437
PspGI CCWGG 4 cut(s) 9, 356, 399, 437
PspN4I GGNNCC 3 cut(s) 277, 373, 405
PspPI GGNCC 2 cut(s) 94, 232
PspPPI RGGWCCY 1 cut(s) 232
PsuI RGATCY 2 cut(s) 447, 456
PvuI CGATCG 1 cut(s) 220
RgaI GCGATCGC 1 cut(s) 220
RseI CAYNNNNRTG 1 cut(s) 89
SacI GAGCTC 1 cut(s) 38
SatI GCNGC 6 cut(s) 264, 435, 510, 513, 522, 549
Sau3AI GATC 7 cut(s) 22, 195, 210, 217, 447, 456, 492
Sau96I GGNCC 2 cut(s) 94, 232
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 4 cut(s) 11, 358, 401, 439
SduI GDGCHC 1 cut(s) 38
SetI ASST 9 cut(s) 38, 99, 176, 234, 297, 339, 377, 474, 534
SfaAI GCGATCGC 1 cut(s) 220
SfcI CTRYAG 1 cut(s) 49
SfoI GGCGCC 1 cut(s) 277
SgfI GCGATCGC 1 cut(s) 220
SgrAI CRCCGGYG 2 cut(s) 251, 551
SinI GGWCC 2 cut(s) 94, 232
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
SphI GCATGC 1 cut(s) 304
Sse9I AATT 3 cut(s) 106, 570, 596
SseBI AGGCCT 1 cut(s) 425
SsiI CCGC 6 cut(s) 79, 87, 435, 510, 546, 549
SspDI GGCGCC 1 cut(s) 275
SstI GAGCTC 1 cut(s) 38
StuI AGGCCT 1 cut(s) 425
StyD4I CCNGG 4 cut(s) 9, 356, 399, 437
StyI CCWWGG 3 cut(s) 69, 591, 609
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 2 cut(s) 297, 534
TaqI TCGA 5 cut(s) 138, 153, 414, 465, 489
TasI AATT 3 cut(s) 106, 570, 596
TauI GCSGC 3 cut(s) 437, 512, 551
TfiI GAWTC 2 cut(s) 14, 605
TseI GCWGC 3 cut(s) 263, 512, 521
TspDTI ATGAA 1 cut(s) 400
TspGWI ACGGA 1 cut(s) 440
VpaK11BI GGWCC 2 cut(s) 94, 232
XagI CCTNNNNNAGG 1 cut(s) 152
XceI RCATGY 2 cut(s) 304, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.