Rh4DG138400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011624
Physical Location & Seq
Forward (+)
27570226 .. 27570651
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG138400.1

Sequence Viewer

Length: 426 bp
ATGATGAACCCGGCGAGCAGTTCAGAGGTTCGCCTCATCGAGAAAGACTCCTTCGCCGCCACCAGAATGATGATCCGAACGATCTACTCCGAGAAGATCAGCGATCACATGGTGGAGGTCCTCCAGGCTAAGAACACCGGTGCTTCTGCTACTGGAAAGGGCGCCCTGTTGTTGCCGGACGATGGCAGGCTTAAGCCCTTCGTGGGCCTTATTATCAAGAATCTCAGCAGTCCCTCCTCTCCCTTGAGACACTTCAGCACCGACGAGGCCAAAGCCCTGGCTCCCTTCATCGAGCGAGAAGCCTTCCTCGCCGACATCCGTGAGGTCTGCAAGCGCATGTTGGAGATCAAACTCAAGAACGCCGCTGCTGGAAACATGGCGTCGAGGACTGAGGAATTGTGGTGTGCTTGCACCAATTGCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.52

Weight (kDa)

8.39

Isoelectric Point (pI)

48.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AciI CCGC 2 cut(s) 57, 363
AclWI GGATC 1 cut(s) 67
AcuI CTGAAG 1 cut(s) 238
AcyI GRCGYC 2 cut(s) 162, 380
AdeI CACNNNGTG 1 cut(s) 112
AfiI CCNNNNNNNGG 2 cut(s) 182, 203
AflII CTTAAG 1 cut(s) 191
AgeI ACCGGT 1 cut(s) 137
AjnI CCWGG 2 cut(s) 123, 276
Alw26I GTCTC 1 cut(s) 241
AlwI GGATC 1 cut(s) 67
AoxI GGCC 2 cut(s) 205, 267
ApeKI GCWGC 1 cut(s) 365
AsiGI ACCGGT 1 cut(s) 137
AspLEI GCGC 2 cut(s) 164, 336
AspS9I GGNCC 2 cut(s) 118, 205
AsuC2I CCSGG 1 cut(s) 11
AvaII GGWCC 1 cut(s) 118
BanI GGYRCC 1 cut(s) 161
BbvI GCAGC 1 cut(s) 352
BccI CCATC 1 cut(s) 176
BciT130I CCWGG 2 cut(s) 125, 278
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 1 cut(s) 241
BfoI RGCGCY 1 cut(s) 165
BfrI CTTAAG 1 cut(s) 191
BisI GCNGC 3 cut(s) 57, 363, 366
BlsI GCNGC 3 cut(s) 58, 364, 367
Bme1390I CCNGG 3 cut(s) 11, 125, 278
Bme18I GGWCC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 118, 205
BmiI GGNNCC 2 cut(s) 163, 282
BmrFI CCNGG 3 cut(s) 11, 125, 278
BplI GAGNNNNNCTC 2 cut(s) 32, 64
BpmI CTGGAG 1 cut(s) 107
BpuEI CTTGAG 2 cut(s) 265, 338
BpuMI CCSGG 1 cut(s) 11
BsaHI GRCGYC 2 cut(s) 162, 380
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 1 cut(s) 137
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 203
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 2 cut(s) 157, 421
BseBI CCWGG 2 cut(s) 125, 278
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 1 cut(s) 315
BseLI CCNNNNNNNGG 2 cut(s) 182, 203
BseMII CTCAG 2 cut(s) 238, 381
BseNI ACTGG 2 cut(s) 157, 421
BseRI GAGGAG 1 cut(s) 226
BseXI GCAGC 1 cut(s) 352
BshFI GGCC 2 cut(s) 207, 269
BshNI GGYRCC 1 cut(s) 161
BshTI ACCGGT 1 cut(s) 137
BsiSI CCGG 3 cut(s) 11, 138, 176
BslFI GGGAC 1 cut(s) 216
BslI CCNNNNNNNGG 2 cut(s) 182, 203
BsmAI GTCTC 1 cut(s) 241
BsmFI GGGAC 1 cut(s) 216
BsnI GGCC 2 cut(s) 207, 269
Bsp143I GATC 5 cut(s) 72, 81, 96, 103, 345
BspACI CCGC 2 cut(s) 57, 363
BspANI GGCC 2 cut(s) 207, 269
BspCNI CTCAG 2 cut(s) 237, 382
BspLI GGNNCC 2 cut(s) 163, 282
BspPI GGATC 1 cut(s) 67
BspT107I GGYRCC 1 cut(s) 161
BspTI CTTAAG 1 cut(s) 191
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 2 cut(s) 157, 421
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 5 cut(s) 72, 81, 96, 103, 345
BssNI GRCGYC 2 cut(s) 162, 380
Bst2UI CCWGG 2 cut(s) 125, 278
BstACI GRCGYC 2 cut(s) 162, 380
BstAFI CTTAAG 1 cut(s) 191
BstAPI GCANNNNNTGC 1 cut(s) 417
BstC8I GCNNGC 4 cut(s) 16, 188, 332, 409
BstDEI CTNAG 3 cut(s) 129, 224, 390
BstF5I GGATG 1 cut(s) 315
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 2 cut(s) 164, 336
BstKTI GATC 5 cut(s) 75, 84, 99, 106, 348
BstMAI GTCTC 1 cut(s) 241
BstMBI GATC 5 cut(s) 72, 81, 96, 103, 345
BstMWI GCNNNNNNNGC 2 cut(s) 308, 417
BstNI CCWGG 2 cut(s) 125, 278
BstNSI RCATGY 1 cut(s) 340
BstSCI CCNGG 3 cut(s) 9, 123, 276
BstV1I GCAGC 1 cut(s) 352
BstXI CCANNNNNNTGG 1 cut(s) 277
BsuRI GGCC 2 cut(s) 207, 269
BtsCI GGATG 1 cut(s) 315
Cac8I GCNNGC 4 cut(s) 16, 188, 332, 409
CfoI GCGC 2 cut(s) 164, 336
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 2 cut(s) 118, 205
CseI GACGC 1 cut(s) 369
CspAI ACCGGT 1 cut(s) 137
CviAII CATG 3 cut(s) 109, 337, 376
CviJI RGCY 8 cut(s) 128, 190, 196, 207, 269, 275, 281, 302
CviKI_1 RGCY 8 cut(s) 128, 190, 196, 207, 269, 275, 281, 302
DdeI CTNAG 3 cut(s) 129, 224, 390
DinI GGCGCC 1 cut(s) 163
DpnI GATC 5 cut(s) 74, 83, 98, 105, 347
DpnII GATC 5 cut(s) 72, 81, 96, 103, 345
DraIII CACNNNGTG 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 118
EcoRII CCWGG 2 cut(s) 123, 276
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
FaeI CATG 3 cut(s) 112, 340, 379
FaiI YATR 3 cut(s) 110, 338, 377
FaqI GGGAC 1 cut(s) 216
FatI CATG 3 cut(s) 108, 336, 375
Fnu4HI GCNGC 3 cut(s) 57, 363, 366
FokI GGATG 1 cut(s) 302
Fsp4HI GCNGC 3 cut(s) 57, 363, 366
GlaI GCGC 2 cut(s) 163, 335
GluI GCNGC 3 cut(s) 57, 363, 366
GsuI CTGGAG 1 cut(s) 107
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 2 cut(s) 207, 269
HapII CCGG 3 cut(s) 11, 138, 176
HgaI GACGC 1 cut(s) 369
HhaI GCGC 2 cut(s) 164, 336
Hin1I GRCGYC 2 cut(s) 162, 380
Hin1II CATG 3 cut(s) 112, 340, 379
Hin6I GCGC 2 cut(s) 162, 334
HinP1I GCGC 2 cut(s) 162, 334
HinfI GANTC 2 cut(s) 47, 220
HpaII CCGG 3 cut(s) 11, 138, 176
Hpy188I TCNGA 3 cut(s) 25, 77, 91
Hpy188III TCNNGA 3 cut(s) 40, 217, 355
Hpy99I CGWCG 2 cut(s) 266, 385
HpyAV CCTTC 4 cut(s) 61, 208, 295, 313
HpyCH4V TGCA 2 cut(s) 330, 411
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 417
HpyF3I CTNAG 3 cut(s) 129, 224, 390
Hsp92I GRCGYC 2 cut(s) 162, 380
Hsp92II CATG 3 cut(s) 112, 340, 379
HspAI GCGC 2 cut(s) 162, 334
KasI GGCGCC 1 cut(s) 161
Kzo9I GATC 5 cut(s) 72, 81, 96, 103, 345
LmnI GCTCC 1 cut(s) 286
Lsp1109I GCAGC 1 cut(s) 352
MalI GATC 5 cut(s) 74, 83, 98, 105, 347
MboI GATC 5 cut(s) 72, 81, 96, 103, 345
MboII GAAGA 1 cut(s) 106
MfeI CAATTG 1 cut(s) 415
MluCI AATT 2 cut(s) 395, 415
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 321
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 365
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 3 cut(s) 11, 138, 176
MspR9I CCNGG 3 cut(s) 11, 125, 278
MunI CAATTG 1 cut(s) 415
MvaI CCWGG 2 cut(s) 125, 278
MwoI GCNNNNNNNGC 2 cut(s) 308, 417
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 1 cut(s) 11
NdeII GATC 5 cut(s) 72, 81, 96, 103, 345
NlaIII CATG 3 cut(s) 112, 340, 379
NlaIV GGNNCC 2 cut(s) 163, 282
NspI RCATGY 1 cut(s) 340
PfeI GAWTC 1 cut(s) 220
PinAI ACCGGT 1 cut(s) 137
PkrI GCNGC 3 cut(s) 58, 364, 367
PleI GAGTC 1 cut(s) 41
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 41
PpuMI RGGWCCY 1 cut(s) 118
Psp5II RGGWCCY 1 cut(s) 118
Psp6I CCWGG 2 cut(s) 123, 276
PspGI CCWGG 2 cut(s) 123, 276
PspN4I GGNNCC 2 cut(s) 163, 282
PspPI GGNCC 2 cut(s) 118, 205
PspPPI RGGWCCY 1 cut(s) 118
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 3 cut(s) 57, 363, 366
Sau3AI GATC 5 cut(s) 72, 81, 96, 103, 345
Sau96I GGNCC 2 cut(s) 118, 205
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 3 cut(s) 11, 125, 278
SetI ASST 3 cut(s) 30, 120, 327
SfoI GGCGCC 1 cut(s) 163
SgrAI CRCCGGYG 1 cut(s) 137
SinI GGWCC 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 65
SmlI CTYRAG 3 cut(s) 191, 244, 353
SmoI CTYRAG 3 cut(s) 191, 244, 353
Sse9I AATT 2 cut(s) 395, 415
SsiI CCGC 2 cut(s) 57, 363
SspDI GGCGCC 1 cut(s) 161
StyD4I CCNGG 3 cut(s) 9, 123, 276
TaqI TCGA 3 cut(s) 39, 291, 383
TasI AATT 2 cut(s) 395, 415
TauI GCSGC 2 cut(s) 59, 365
TfiI GAWTC 1 cut(s) 220
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseI GCWGC 1 cut(s) 365
TspDTI ATGAA 2 cut(s) 20, 277
TspGWI ACGGA 1 cut(s) 308
Vha464I CTTAAG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 118
XceI RCATGY 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.