Rh5AG346300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0005842
Physical Location & Seq
Forward (+)
57116254 .. 57121557
5304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG346300.1

Sequence Viewer

Length: 480 bp
ATGGGTCACCAAAGCCCGACTCCAGTGCCACGTAAACACCACTCACCAGACAAGAATTCATCGGACGACGCCTCTGCAATCAGCAGAGCGAAGAAGAGATGCTACCTAGTAGACTACGATGCCAGCGGTGATCGGGTCGAGTGCTCCAGCAAGTACTGCCGGTCATGCAGCGCCGGCATGATCGCCGATTGTGTGGCGCTGTGCTGCTGCCCTTGCGCTCTGGTCAACCTGTTGACTCTGGCCTTCGTGAAAGTGTCGTGGATGGTTGGGAGGAAGTGCTTGGGGTTAGGCAAGAACAAGAAGAAGGAAAACCAGAGCCAGGGGCAGAATCGGAAATCTAAAAAAAAGTGCTGGAGCAAGAAGAAGCAGTTTGGAAATTTGAAGAATCAGGCAAACACGGGGAAGCAAAGTAAATTAGTCGTAGAAGAACTCACCGGCAACAAAGACGACGTTAATGGCAACCTGGATTATTGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.52

Weight (kDa)

9.41

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 111
AciI CCGC 1 cut(s) 126
AcsI RAATTY 2 cut(s) 55, 376
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 319
AgsI TTSAA 1 cut(s) 382
AjnI CCWGG 2 cut(s) 318, 462
Alw21I GWGCWC 1 cut(s) 146
AoxI GGCC 1 cut(s) 240
ApeKI GCWGC 3 cut(s) 168, 204, 207
ApoI RAATTY 2 cut(s) 55, 376
AspLEI GCGC 3 cut(s) 173, 199, 218
AsuHPI GGTGA 3 cut(s) 36, 140, 424
BarI GAAGNNNNNNTAC 2 cut(s) 86, 118
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 3 cut(s) 180, 191, 194
BccI CCATC 1 cut(s) 256
BcgI CGANNNNNNTGC 4 cut(s) 7, 41, 56, 90
BciT130I CCWGG 2 cut(s) 320, 464
BfaI CTAG 1 cut(s) 107
BfoI RGCGCY 2 cut(s) 174, 200
BisI GCNGC 3 cut(s) 169, 205, 208
BlsI GCNGC 3 cut(s) 170, 206, 209
BmcAI AGTACT 1 cut(s) 155
Bme1390I CCNGG 2 cut(s) 320, 464
BmrFI CCNGG 2 cut(s) 320, 464
BmsI GCATC 2 cut(s) 89, 109
BpmI CTGGAG 3 cut(s) 6, 130, 373
BsaAI YACGTR 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 319
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse118I RCCGGY 3 cut(s) 159, 173, 434
Bse1I ACTGG 1 cut(s) 23
BseBI CCWGG 2 cut(s) 320, 464
BseDI CCNNGG 1 cut(s) 319
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 1 cut(s) 319
BseNI ACTGG 1 cut(s) 23
BseXI GCAGC 3 cut(s) 180, 191, 194
BshFI GGCC 1 cut(s) 242
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 3 cut(s) 160, 174, 435
BslI CCNNNNNNNGG 1 cut(s) 319
BsnI GGCC 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 146
Bsp143I GATC 2 cut(s) 130, 180
BspACI CCGC 1 cut(s) 126
BspANI GGCC 1 cut(s) 242
BsrFI RCCGGY 3 cut(s) 159, 173, 434
BsrI ACTGG 1 cut(s) 23
BssAI RCCGGY 3 cut(s) 159, 173, 434
BssECI CCNNGG 1 cut(s) 319
BssMI GATC 2 cut(s) 130, 180
BssNI GRCGYC 1 cut(s) 69
Bst2UI CCWGG 2 cut(s) 320, 464
Bst6I CTCTTC 1 cut(s) 89
BstACI GRCGYC 1 cut(s) 69
BstAPI GCANNNNNTGC 1 cut(s) 156
BstBAI YACGTR 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 124, 175
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 1 cut(s) 267
BstH2I RGCGCY 2 cut(s) 174, 200
BstHHI GCGC 3 cut(s) 173, 199, 218
BstKTI GATC 2 cut(s) 133, 183
BstMBI GATC 2 cut(s) 130, 180
BstMWI GCNNNNNNNGC 4 cut(s) 156, 165, 174, 213
BstNI CCWGG 2 cut(s) 320, 464
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 2 cut(s) 318, 462
BstV1I GCAGC 3 cut(s) 180, 191, 194
BsuRI GGCC 1 cut(s) 242
BtsCI GGATG 1 cut(s) 267
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 124, 175
CfoI GCGC 3 cut(s) 173, 199, 218
Cfr10I RCCGGY 3 cut(s) 159, 173, 434
CseI GACGC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 154
CviAII CATG 2 cut(s) 165, 178
CviJI RGCY 3 cut(s) 15, 242, 318
CviKI_1 RGCY 3 cut(s) 15, 242, 318
CviQI GTAC 1 cut(s) 154
DpnI GATC 2 cut(s) 132, 182
DpnII GATC 2 cut(s) 130, 180
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Eco91I GGTNACC 1 cut(s) 5
EcoO65I GGTNACC 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 318, 462
FaeI CATG 2 cut(s) 168, 181
FaiI YATR 2 cut(s) 166, 179
FatI CATG 2 cut(s) 164, 177
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 3 cut(s) 169, 205, 208
FokI GGATG 1 cut(s) 274
Fsp4HI GCNGC 3 cut(s) 169, 205, 208
FspBI CTAG 1 cut(s) 107
GlaI GCGC 3 cut(s) 172, 198, 217
GluI GCNGC 3 cut(s) 169, 205, 208
GsuI CTGGAG 3 cut(s) 6, 130, 373
HaeII RGCGCY 2 cut(s) 174, 200
HaeIII GGCC 1 cut(s) 242
HapII CCGG 3 cut(s) 160, 174, 435
HgaI GACGC 1 cut(s) 77
HhaI GCGC 3 cut(s) 173, 199, 218
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 2 cut(s) 168, 181
Hin6I GCGC 3 cut(s) 171, 197, 216
HinP1I GCGC 3 cut(s) 171, 197, 216
HincII GTYRAC 2 cut(s) 226, 234
HindII GTYRAC 2 cut(s) 226, 234
HinfI GANTC 4 cut(s) 19, 235, 328, 385
HpaII CCGG 3 cut(s) 160, 174, 435
HphI GGTGA 3 cut(s) 36, 140, 424
Hpy166II GTNNAC 4 cut(s) 35, 112, 226, 234
Hpy188I TCNGA 2 cut(s) 64, 333
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 4 cut(s) 35, 112, 226, 234
Hpy99I CGWCG 2 cut(s) 71, 452
HpyAV CCTTC 2 cut(s) 253, 298
HpyCH4IV ACGT 2 cut(s) 31, 450
HpyCH4V TGCA 2 cut(s) 77, 168
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 165, 174, 213
HpySE526I ACGT 2 cut(s) 31, 450
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 2 cut(s) 168, 181
HspAI GCGC 3 cut(s) 171, 197, 216
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 130, 180
LmnI GCTCC 2 cut(s) 149, 354
Lsp1109I GCAGC 3 cut(s) 180, 191, 194
LweI GCATC 2 cut(s) 89, 109
MaeI CTAG 1 cut(s) 107
MaeII ACGT 2 cut(s) 31, 450
MaeIII GTNAC 1 cut(s) 5
MalI GATC 2 cut(s) 132, 182
MboI GATC 2 cut(s) 130, 180
MboII GAAGA 6 cut(s) 103, 106, 313, 373, 394, 437
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 3 cut(s) 55, 376, 413
MlyI GAGTC 2 cut(s) 13, 229
MnlI CCTC 2 cut(s) 82, 264
MroNI GCCGGC 1 cut(s) 173
MseI TTAA 1 cut(s) 453
MspA1I CMGCKG 1 cut(s) 126
MspI CCGG 3 cut(s) 160, 174, 435
MspR9I CCNGG 2 cut(s) 320, 464
MvaI CCWGG 2 cut(s) 320, 464
MwoI GCNNNNNNNGC 4 cut(s) 156, 165, 174, 213
NaeI GCCGGC 1 cut(s) 175
NdeII GATC 2 cut(s) 130, 180
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 2 cut(s) 168, 181
NmuCI GTSAC 1 cut(s) 5
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 2 cut(s) 328, 385
PkrI GCNGC 3 cut(s) 170, 206, 209
PleI GAGTC 2 cut(s) 13, 229
PpsI GAGTC 2 cut(s) 13, 229
Ppu21I YACGTR 1 cut(s) 32
Psp6I CCWGG 2 cut(s) 318, 462
PspEI GGTNACC 1 cut(s) 5
PspGI CCWGG 2 cut(s) 318, 462
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 3 cut(s) 169, 205, 208
Sau3AI GATC 2 cut(s) 130, 180
ScaI AGTACT 1 cut(s) 155
SchI GAGTC 2 cut(s) 13, 229
ScrFI CCNGG 2 cut(s) 320, 464
SduI GDGCHC 1 cut(s) 146
SetI ASST 5 cut(s) 34, 108, 231, 453, 465
SfaNI GCATC 2 cut(s) 89, 109
Sse9I AATT 3 cut(s) 55, 376, 413
SsiI CCGC 1 cut(s) 126
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 2 cut(s) 318, 462
TaiI ACGT 2 cut(s) 34, 453
TaqI TCGA 1 cut(s) 138
TasI AATT 3 cut(s) 55, 376, 413
TatI WGTACW 1 cut(s) 153
TfiI GAWTC 2 cut(s) 328, 385
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 3 cut(s) 168, 204, 207
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 1 cut(s) 48
TspRI CASTG 1 cut(s) 30
XapI RAATTY 2 cut(s) 55, 376
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 1 cut(s) 107
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.