Rw5G032700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005842
Physical Location & Seq
Forward (+)
55432448 .. 55433113
666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032700.1

Sequence Viewer

Length: 666 bp
ATGGGTCACCAAAGCCCGACTCCGGTGCCACGTAAACACCACTCACCAGACTCGAGTTCATCGGATGGCGCCGCCGCAATCAGTAGAGGGAAGAAGAGATGCTACCTAGTAGACTACGGCGCCAGCGGTGATAGGGTCGAGTGCTCCGGCAAGTACTGCCGGTCATGCAGCGCCTGCATGATCGCCGATTGCGTGGCGCTGTGCTGCTGCCCTTGTGCTCTGGTCAACCTGTTGACTCTGGCCTTCGTGAAAGTGTCGTGGATGGTTGGGAGGAAGTGCTTGGGGTTAGGCAAGAACAAGAAGAAGGAAAACCAGAGCCAGGGGCAGAATCGGAAATCCAAAAAAAAGTGCTGGAGCAAGAAGAAGCAGGTTAGCAGTAGTCATCGGCATCAACGTTGTGCGGCGGAGGAGGAATTGTCGGCGCAGATATCGTGCTACGTGTACGGTGTTGATGATCAGGAGGAGGAAGAAAAGGAGGATTGTGGGTTGCGGATTAGTGCCTTCACCATTCTTTTTTATGATGAAAATTCAAGTACAAAGCTTAAAGTGTGGGTTGCCGAAGTGGAAACTGGGAGGGAGGAGGAGAGAGAGAGTCTGAGATTTGGATCGAGGGAGAGAGAGTGTCTGTGTGTTTTAGATGTAGGCTGTGTAGGGGTGATTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

24.61

Weight (kDa)

8.49

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 358
AccB1I GGYRCC 3 cut(s) 25, 68, 119
AccI GTMKAC 1 cut(s) 111
AciI CCGC 6 cut(s) 72, 75, 126, 401, 404, 490
AclI AACGTT 1 cut(s) 394
AclWI GGATC 1 cut(s) 613
AcsI RAATTY 1 cut(s) 526
AcyI GRCGYC 2 cut(s) 69, 120
AfaI GTAC 3 cut(s) 155, 443, 535
AfiI CCNNNNNNNGG 2 cut(s) 22, 319
AflIII ACRYGT 1 cut(s) 438
AgsI TTSAA 1 cut(s) 531
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 1 cut(s) 541
AluI AGCT 1 cut(s) 541
Alw21I GWGCWC 2 cut(s) 146, 220
AlwI GGATC 1 cut(s) 613
AlwNI CAGNNNCTG 1 cut(s) 174
Ama87I CYCGRG 1 cut(s) 52
AoxI GGCC 1 cut(s) 240
ApeKI GCWGC 3 cut(s) 168, 204, 207
ApoI RAATTY 1 cut(s) 526
AspLEI GCGC 5 cut(s) 71, 122, 173, 199, 424
AsuHPI GGTGA 3 cut(s) 36, 140, 496
AvaI CYCGRG 1 cut(s) 52
BanI GGYRCC 3 cut(s) 25, 68, 119
BarI GAAGNNNNNNTAC 2 cut(s) 86, 118
Bbv12I GWGCWC 2 cut(s) 146, 220
BbvI GCAGC 3 cut(s) 180, 191, 194
BccI CCATC 2 cut(s) 59, 256
BceAI ACGGC 1 cut(s) 133
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BciT130I CCWGG 1 cut(s) 320
BclI TGATCA 1 cut(s) 454
BfaI CTAG 1 cut(s) 107
BfoI RGCGCY 4 cut(s) 72, 123, 174, 200
BfuAI ACCTGC 1 cut(s) 358
BisI GCNGC 6 cut(s) 72, 75, 169, 205, 208, 402
BlsI GCNGC 6 cut(s) 73, 76, 170, 206, 209, 403
BmcAI AGTACT 1 cut(s) 155
Bme1390I CCNGG 1 cut(s) 320
BmeT110I CYCGRG 1 cut(s) 52
BmiI GGNNCC 3 cut(s) 27, 70, 121
BmrFI CCNGG 1 cut(s) 320
BmrI ACTGGG 1 cut(s) 579
BmsI GCATC 2 cut(s) 89, 397
BmuI ACTGGG 1 cut(s) 579
BpmI CTGGAG 1 cut(s) 373
BsaAI YACGTR 2 cut(s) 32, 439
BsaBI GATNNNNATC 1 cut(s) 604
BsaHI GRCGYC 2 cut(s) 69, 120
BsaJI CCNNGG 1 cut(s) 319
BsaWI WCCGGW 1 cut(s) 22
BsaXI ACNNNNNCTCC 4 cut(s) 401, 431, 605, 635
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 319
Bse118I RCCGGY 1 cut(s) 159
Bse1I ACTGG 1 cut(s) 574
Bse8I GATNNNNATC 1 cut(s) 604
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 1 cut(s) 319
BseGI GGATG 2 cut(s) 70, 267
BseJI GATNNNNATC 1 cut(s) 604
BseLI CCNNNNNNNGG 2 cut(s) 22, 319
BseMII CTCAG 1 cut(s) 587
BseNI ACTGG 1 cut(s) 574
BseRI GAGGAG 4 cut(s) 422, 476, 593, 596
BseXI GCAGC 3 cut(s) 180, 191, 194
BshFI GGCC 1 cut(s) 242
BshNI GGYRCC 3 cut(s) 25, 68, 119
BsiHKAI GWGCWC 2 cut(s) 146, 220
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 3 cut(s) 23, 147, 160
BslI CCNNNNNNNGG 2 cut(s) 22, 319
BsnI GGCC 1 cut(s) 242
BsoBI CYCGRG 1 cut(s) 52
Bsp1286I GDGCHC 2 cut(s) 146, 220
Bsp143I GATC 3 cut(s) 180, 454, 605
BspACI CCGC 6 cut(s) 72, 75, 126, 401, 404, 490
BspANI GGCC 1 cut(s) 242
BspCNI CTCAG 1 cut(s) 588
BspLI GGNNCC 3 cut(s) 27, 70, 121
BspMI ACCTGC 1 cut(s) 358
BspPI GGATC 1 cut(s) 613
BspT107I GGYRCC 3 cut(s) 25, 68, 119
BsrFI RCCGGY 1 cut(s) 159
BsrI ACTGG 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 1 cut(s) 319
BssMI GATC 3 cut(s) 180, 454, 605
BssNI GRCGYC 2 cut(s) 69, 120
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 1 cut(s) 446
Bst6I CTCTTC 1 cut(s) 89
BstACI GRCGYC 2 cut(s) 69, 120
BstAPI GCANNNNNTGC 2 cut(s) 156, 174
BstBAI YACGTR 2 cut(s) 32, 439
BstC8I GCNNGC 2 cut(s) 124, 175
BstDEI CTNAG 1 cut(s) 596
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 2 cut(s) 70, 267
BstH2I RGCGCY 4 cut(s) 72, 123, 174, 200
BstHHI GCGC 5 cut(s) 71, 122, 173, 199, 424
BstKTI GATC 3 cut(s) 183, 457, 608
BstMBI GATC 3 cut(s) 180, 454, 605
BstMWI GCNNNNNNNGC 3 cut(s) 156, 165, 174
BstNI CCWGG 1 cut(s) 320
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 1 cut(s) 318
BstV1I GCAGC 3 cut(s) 180, 191, 194
BsuRI GGCC 1 cut(s) 242
BtsCI GGATG 2 cut(s) 70, 267
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 2 cut(s) 124, 175
CaiI CAGNNNCTG 1 cut(s) 174
CfoI GCGC 5 cut(s) 71, 122, 173, 199, 424
Cfr10I RCCGGY 1 cut(s) 159
Csp6I GTAC 3 cut(s) 154, 442, 534
CviAII CATG 2 cut(s) 165, 178
CviJI RGCY 5 cut(s) 15, 242, 318, 541, 645
CviKI_1 RGCY 5 cut(s) 15, 242, 318, 541, 645
CviQI GTAC 3 cut(s) 154, 442, 534
DdeI CTNAG 1 cut(s) 596
DinI GGCGCC 2 cut(s) 70, 121
DpnI GATC 3 cut(s) 182, 456, 607
DpnII GATC 3 cut(s) 180, 454, 605
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
EciI GGCGGA 1 cut(s) 419
Eco32I GATATC 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 52
Eco91I GGTNACC 1 cut(s) 5
EcoO65I GGTNACC 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 318
EcoRV GATATC 1 cut(s) 429
EgeI GGCGCC 2 cut(s) 70, 121
EheI GGCGCC 2 cut(s) 70, 121
FaeI CATG 2 cut(s) 168, 181
FaiI YATR 3 cut(s) 166, 179, 519
FatI CATG 2 cut(s) 164, 177
FbaI TGATCA 1 cut(s) 454
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 6 cut(s) 72, 75, 169, 205, 208, 402
FokI GGATG 2 cut(s) 77, 274
Fsp4HI GCNGC 6 cut(s) 72, 75, 169, 205, 208, 402
FspBI CTAG 1 cut(s) 107
GlaI GCGC 5 cut(s) 70, 121, 172, 198, 423
GluI GCNGC 6 cut(s) 72, 75, 169, 205, 208, 402
GsuI CTGGAG 1 cut(s) 373
HaeII RGCGCY 4 cut(s) 72, 123, 174, 200
HaeIII GGCC 1 cut(s) 242
HapII CCGG 3 cut(s) 23, 147, 160
HhaI GCGC 5 cut(s) 71, 122, 173, 199, 424
Hin1I GRCGYC 2 cut(s) 69, 120
Hin1II CATG 2 cut(s) 168, 181
Hin6I GCGC 5 cut(s) 69, 120, 171, 197, 422
HinP1I GCGC 5 cut(s) 69, 120, 171, 197, 422
HincII GTYRAC 2 cut(s) 226, 234
HindII GTYRAC 2 cut(s) 226, 234
HindIII AAGCTT 1 cut(s) 539
HinfI GANTC 5 cut(s) 19, 50, 235, 328, 592
HpaII CCGG 3 cut(s) 23, 147, 160
HphI GGTGA 3 cut(s) 36, 140, 496
Hpy166II GTNNAC 5 cut(s) 35, 112, 226, 234, 442
Hpy188I TCNGA 3 cut(s) 64, 333, 597
Hpy188III TCNNGA 2 cut(s) 247, 458
Hpy8I GTNNAC 5 cut(s) 35, 112, 226, 234, 442
HpyAV CCTTC 3 cut(s) 253, 298, 511
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4IV ACGT 3 cut(s) 31, 394, 438
HpyCH4V TGCA 2 cut(s) 168, 177
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 165, 174
HpyF3I CTNAG 1 cut(s) 596
HpySE526I ACGT 3 cut(s) 31, 394, 438
Hsp92I GRCGYC 2 cut(s) 69, 120
Hsp92II CATG 2 cut(s) 168, 181
HspAI GCGC 5 cut(s) 69, 120, 171, 197, 422
KasI GGCGCC 2 cut(s) 68, 119
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 3 cut(s) 180, 454, 605
LmnI GCTCC 2 cut(s) 149, 354
Lsp1109I GCAGC 3 cut(s) 180, 191, 194
LweI GCATC 2 cut(s) 89, 397
MaeI CTAG 1 cut(s) 107
MaeII ACGT 3 cut(s) 31, 394, 438
MaeIII GTNAC 1 cut(s) 5
MalI GATC 3 cut(s) 182, 456, 607
MboI GATC 3 cut(s) 180, 454, 605
MboII GAAGA 5 cut(s) 103, 106, 313, 373, 479
MhlI GDGCHC 2 cut(s) 146, 220
MluCI AATT 2 cut(s) 413, 526
Mly113I GGCGCC 2 cut(s) 69, 120
MlyI GAGTC 4 cut(s) 13, 44, 229, 601
MseI TTAA 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 126
MspI CCGG 3 cut(s) 23, 147, 160
MspR9I CCNGG 1 cut(s) 320
MvaI CCWGG 1 cut(s) 320
MwoI GCNNNNNNNGC 3 cut(s) 156, 165, 174
NarI GGCGCC 2 cut(s) 69, 120
NdeII GATC 3 cut(s) 180, 454, 605
NlaIII CATG 2 cut(s) 168, 181
NlaIV GGNNCC 3 cut(s) 27, 70, 121
NmuCI GTSAC 1 cut(s) 5
PaeR7I CTCGAG 1 cut(s) 52
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 391
PfeI GAWTC 1 cut(s) 328
PkrI GCNGC 6 cut(s) 73, 76, 170, 206, 209, 403
PleI GAGTC 4 cut(s) 13, 44, 229, 600
PluTI GGCGCC 2 cut(s) 72, 123
PpsI GAGTC 4 cut(s) 13, 44, 229, 600
Ppu21I YACGTR 2 cut(s) 32, 439
Psp1406I AACGTT 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 318
PspEI GGTNACC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 3 cut(s) 27, 70, 121
PspXI VCTCGAGB 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 174
RsaI GTAC 3 cut(s) 155, 443, 535
RsaNI GTAC 3 cut(s) 154, 442, 534
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 6 cut(s) 72, 75, 169, 205, 208, 402
Sau3AI GATC 3 cut(s) 180, 454, 605
ScaI AGTACT 1 cut(s) 155
SchI GAGTC 4 cut(s) 13, 44, 229, 601
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 2 cut(s) 146, 220
SetI ASST 7 cut(s) 34, 108, 231, 372, 397, 441, 543
SfaNI GCATC 2 cut(s) 89, 397
SfoI GGCGCC 2 cut(s) 70, 121
Sfr274I CTCGAG 1 cut(s) 52
SlaI CTCGAG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 2 cut(s) 413, 526
SsiI CCGC 6 cut(s) 72, 75, 126, 401, 404, 490
SspDI GGCGCC 2 cut(s) 68, 119
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 1 cut(s) 318
TaaI ACNGT 1 cut(s) 446
TaiI ACGT 3 cut(s) 34, 397, 441
TaqI TCGA 3 cut(s) 53, 138, 608
TasI AATT 2 cut(s) 413, 526
TatI WGTACW 2 cut(s) 153, 533
TauI GCSGC 3 cut(s) 74, 77, 404
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 3 cut(s) 168, 204, 207
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 2 cut(s) 48, 537
XapI RAATTY 1 cut(s) 526
XhoI CTCGAG 1 cut(s) 52
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 1 cut(s) 107
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.