Rh5AG524800

PRLI-interacting factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007381
Physical Location & Seq
Forward (+)
90002502 .. 90006195
3694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG524800.1

Sequence Viewer

Length: 1749 bp
ATGAACGATAAGTCACAGTTGATGAATCAACCCCCGCCGCCGATGATGAGTCTGAGCCAAGCTCAAGCCATGAGCCAGCCGCCGCCGATGATGGCTCAGCCGCCGCCGCCGCAGATGATGGCTCCGTCTTCTCAAGCCATGGCTGCTGCTGTTCAGCAGCAACAGATGATGATGAACAGTCAATCTCAGCCGTTGATGATGAACCGGAGCTACAAGGCTTGGCCGCCGCAGCAGCAGCAGCAACAGTTCGTCGCCGCCCAGAAGCCGCCGCAGTCGAATCTGGGAAGGAATAATTGGAAGGGTAAGAAGGGAAATGACAAGCGGAGAATGGAGAAAAACGTTCCCAATTTGCCCCTGAGCGGTGGCCAGAGCAGTAGCGGAGGAGGGTATAATGTTCCCAATTTGCCCCTGAGTGGTGGCCAGAGCAGCGGAGGAGGGTATATACCTCCGACGCTTAACGAGTTACAGTCACAGAATCGAATGAAAGCTCGGAAATTTTACCCCAAAAAGAAGTTTAACAATAGGTTTGCGCCTTACGCCCCAAGGAACACGACGTCGTTTATCATTAGAGCTAAGAAGTCCGGTGGGATTGCTTCGCTGGTTTCGCCTTGTCCGGTGACCCCTGCCGTGCTCCCCACGCCCATTTTCTCGCCGTCGAGGGAGGTTTTGGGGGACACGGCGAAGGAGGAGTGGGGTGTGGATGGGTATGGGTCGATGAAAGGGTTGATTCGGCTTCGGTCTCCGGGGAATGATGATGATGAGGAGGGAGGGGGTGGGTCGAGTGAGAGTGATGTGGAGGAGCATGTGGAGGTGGAGAGGAGGTTGGACCATGATTTGAGCCGGTTCGAGATGATATACCCGAATTACAGTGGGGTTGAGTATAATAATGTGCTGGAGAATAGAGTGGATGATCAGGATACACACATTGCTCAGTTGGAGGAAGAGAATTTGACTTTGAAGGAGAGGCTGTTCTTGATGGAGAGGGAGTTGGGGGACTTGAGGAGGAGGATGTTGTTTCTTGAGAGGAGGCACCATGTGATGGAGGAGGTTAATGAGGAGGTTGTGGAGAATGGGTCGGAGAACGATAGTGATGGAGGGTCGGATGTGCCCCATATGGGCATGGGGGGAGAGAGCAACATTGAGGTGGTAGATTATGTGTCCAGAAACGGTGGGGTTAGGGATGTTTCGATGGAAGAATATGTGCATGGTGAAATGAATTTAAAGGAAAATGAGGTCAAAGATGAAGTGAGAAATGAGGTTGTAGGTAATGAAGAGATTGAACGTAAGAACGAGGGAAGAAGGAATGACCTTGTAGGTGATGGGGTCGTTTCAGAGAAGGTTGAGGAAGCGAGGAATGAGTTTATAATGAACAAAGTAATTGCAAATAAAACTGAGCTGGTGAGTAGTGAAGTTGAGACAAAGAATGAGAATGAGTTTATGCAGGGTCAGGTGAACATAGAGAAGGCTGAAGACATGGTAATTGCCTGCAATGAGAAGAATGAAACTAAAGTTATGAGAAATGAAGTTGAGACCAAGATTGAAAATGAGTTGATGCGTGGTCAGATGAATGCAGATAAGGATGAAGAAATGGGAACTTCATGCACAGAGAATAATAAGACCGAGGGTCAGGTGAAGAATGAAGTTGAGACCAAGAATGAGAGAGAGTTGATGCAGGGTCAGATGAATGCACAGAGGGATGAAGAAATGGGAATTGCTAGCACAGAGAAGAGCATTGGCGTCGGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

582

Amino Acids

65.21

Weight (kDa)

4.9

Isoelectric Point (pI)

62.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1364
AatII GACGTC 1 cut(s) 557
AccB1I GGYRCC 1 cut(s) 1029
AccB7I CCANNNNNTGG 1 cut(s) 1039
AccBSI CCGCTC 1 cut(s) 360
AclI AACGTT 1 cut(s) 339
AcoI YGGCCR 3 cut(s) 221, 364, 418
AcsI RAATTY 3 cut(s) 494, 946, 1216
AcuI CTGAAG 1 cut(s) 1488
AcyI GRCGYC 2 cut(s) 554, 1737
AdeI CACNNNGTG 1 cut(s) 1036
AfiI CCNNNNNNNGG 4 cut(s) 359, 413, 1039, 1115
AgsI TTSAA 3 cut(s) 958, 1280, 1541
AhdI GACNNNNNGTC 1 cut(s) 1623
AjuI GAANNNNNNNTTGG 2 cut(s) 277, 309
AloI GAACNNNNNNTCC 2 cut(s) 953, 985
AluBI AGCT 5 cut(s) 62, 210, 488, 572, 1396
AluI AGCT 5 cut(s) 62, 210, 488, 572, 1396
Alw21I GWGCWC 1 cut(s) 633
Alw26I GTCTC 4 cut(s) 744, 1409, 1523, 1640
AoxI GGCC 3 cut(s) 221, 364, 418
ApeKI GCWGC 8 cut(s) 143, 146, 157, 229, 232, 235, 238, 426
ApoI RAATTY 3 cut(s) 494, 946, 1216
AspLEI GCGC 1 cut(s) 532
AspS9I GGNCC 1 cut(s) 826
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 6 cut(s) 628, 1220, 1328, 1411, 1462, 1642
AsuNHI GCTAGC 1 cut(s) 1715
AvaII GGWCC 1 cut(s) 826
BaeGI GKGCMC 1 cut(s) 1110
BalI TGGCCA 2 cut(s) 366, 420
BanI GGYRCC 1 cut(s) 1029
BbsI GAAGAC 2 cut(s) 120, 1476
Bbv12I GWGCWC 1 cut(s) 633
BbvI GCAGC 8 cut(s) 130, 133, 169, 241, 244, 247, 250, 438
BccI CCATC 8 cut(s) 85, 112, 695, 970, 1033, 1085, 1183, 1313
BceAI ACGGC 4 cut(s) 175, 611, 637, 693
BcgI CGANNNNNNTGC 1 cut(s) 1720
BciVI GTATCC 1 cut(s) 910
BclI TGATCA 1 cut(s) 910
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 4 cut(s) 744, 1409, 1523, 1640
BfaI CTAG 1 cut(s) 1716
BfuI GTATCC 1 cut(s) 910
BlpI GCTNAGC 1 cut(s) 96
Bme1390I CCNGG 1 cut(s) 744
Bme18I GGWCC 1 cut(s) 826
BmeRI GACNNNNNGTC 1 cut(s) 1623
BmgT120I GGNCC 1 cut(s) 826
BmiI GGNNCC 2 cut(s) 123, 1031
BmrFI CCNGG 1 cut(s) 744
BmsI GCATC 2 cut(s) 1542, 1659
BmtI GCTAGC 1 cut(s) 1719
BpiI GAAGAC 2 cut(s) 120, 1476
BplI GAGNNNNNCTC 2 cut(s) 46, 78
BpmI CTGGAG 1 cut(s) 914
Bpu10I CCTNAGC 1 cut(s) 356
Bpu1102I GCTNAGC 1 cut(s) 96
BpuEI CTTGAG 4 cut(s) 48, 117, 1018, 1040
BpuMI CCSGG 1 cut(s) 744
BsaHI GRCGYC 2 cut(s) 554, 1737
BsaI GGTCTC 3 cut(s) 744, 1523, 1640
BsaJI CCNNGG 4 cut(s) 138, 542, 743, 1620
BsaWI WCCGGW 3 cut(s) 204, 581, 613
BsaXI ACNNNNNCTCC 8 cut(s) 806, 836, 953, 971, 983, 1001, 1058, 1088
Bsc4I CCNNNNNNNGG 4 cut(s) 359, 413, 1039, 1115
Bse118I RCCGGY 1 cut(s) 840
Bse3DI GCAATG 2 cut(s) 924, 1495
BseDI CCNNGG 4 cut(s) 138, 542, 743, 1620
BseGI GGATG 7 cut(s) 706, 913, 1014, 1108, 1186, 1585, 1702
BseLI CCNNNNNNNGG 4 cut(s) 359, 413, 1039, 1115
BseMI GCAATG 2 cut(s) 924, 1495
BseMII CTCAG 7 cut(s) 44, 110, 200, 347, 401, 944, 1383
BseSI GKGCMC 1 cut(s) 1110
BseXI GCAGC 8 cut(s) 130, 133, 169, 241, 244, 247, 250, 438
BshFI GGCC 3 cut(s) 223, 366, 420
BshNI GGYRCC 1 cut(s) 1029
BsiHKAI GWGCWC 1 cut(s) 633
BsiSI CCGG 5 cut(s) 205, 582, 614, 743, 841
BslFI GGGAC 2 cut(s) 686, 1007
BslI CCNNNNNNNGG 4 cut(s) 359, 413, 1039, 1115
BsmAI GTCTC 4 cut(s) 744, 1409, 1523, 1640
BsmFI GGGAC 2 cut(s) 686, 1007
BsmI GAATGC 2 cut(s) 1573, 1690
BsnI GGCC 3 cut(s) 223, 366, 420
Bso31I GGTCTC 3 cut(s) 744, 1523, 1640
Bsp1286I GDGCHC 2 cut(s) 633, 1110
Bsp143I GATC 1 cut(s) 910
Bsp1720I GCTNAGC 1 cut(s) 96
Bsp19I CCATGG 1 cut(s) 138
BspANI GGCC 3 cut(s) 223, 366, 420
BspCNI CTCAG 7 cut(s) 45, 109, 199, 348, 402, 943, 1384
BspLI GGNNCC 2 cut(s) 123, 1031
BspOI GCTAGC 1 cut(s) 1719
BspQI GCTCTTC 1 cut(s) 1721
BspT107I GGYRCC 1 cut(s) 1029
BspTNI GGTCTC 3 cut(s) 744, 1523, 1640
BsrBI CCGCTC 1 cut(s) 360
BsrDI GCAATG 2 cut(s) 924, 1495
BsrFI RCCGGY 1 cut(s) 840
BssAI RCCGGY 1 cut(s) 840
BssECI CCNNGG 4 cut(s) 138, 542, 743, 1620
BssMI GATC 1 cut(s) 910
BssNI GRCGYC 2 cut(s) 554, 1737
BssT1I CCWWGG 2 cut(s) 138, 542
Bst4CI ACNGT 6 cut(s) 18, 179, 246, 468, 869, 1169
Bst6I CTCTTC 3 cut(s) 936, 1266, 1721
BstACI GRCGYC 2 cut(s) 554, 1737
BstC8I GCNNGC 3 cut(s) 77, 1486, 1717
BstDEI CTNAG 8 cut(s) 53, 96, 186, 356, 410, 573, 930, 1392
BstDSI CCRYGG 1 cut(s) 138
BstEII GGTNACC 1 cut(s) 616
BstF5I GGATG 7 cut(s) 706, 913, 1014, 1108, 1186, 1585, 1702
BstHHI GCGC 1 cut(s) 532
BstKTI GATC 1 cut(s) 913
BstMAI GTCTC 4 cut(s) 744, 1409, 1523, 1640
BstMBI GATC 1 cut(s) 910
BstNSI RCATGY 1 cut(s) 806
BstPI GGTNACC 1 cut(s) 616
BstSCI CCNGG 1 cut(s) 742
BstSLI GKGCMC 1 cut(s) 1110
BstV1I GCAGC 8 cut(s) 130, 133, 169, 241, 244, 247, 250, 438
BstV2I GAAGAC 2 cut(s) 120, 1476
BsuI GTATCC 1 cut(s) 910
BsuRI GGCC 3 cut(s) 223, 366, 420
BtgI CCRYGG 1 cut(s) 138
BtsCI GGATG 7 cut(s) 706, 913, 1014, 1108, 1186, 1585, 1702
BtsIMutI CAGTG 1 cut(s) 874
Cac8I GCNNGC 3 cut(s) 77, 1486, 1717
CfoI GCGC 1 cut(s) 532
Cfr10I RCCGGY 1 cut(s) 840
Cfr13I GGNCC 1 cut(s) 826
CseI GACGC 2 cut(s) 460, 1726
CviAII CATG 9 cut(s) 70, 139, 803, 830, 1034, 1120, 1205, 1474, 1599
DdeI CTNAG 8 cut(s) 53, 96, 186, 356, 410, 573, 930, 1392
DpnI GATC 1 cut(s) 912
DpnII GATC 1 cut(s) 910
DraI TTTAAA 1 cut(s) 1221
DraIII CACNNNGTG 1 cut(s) 1036
DriI GACNNNNNGTC 1 cut(s) 1623
EaeI YGGCCR 3 cut(s) 221, 364, 418
Eam1104I CTCTTC 3 cut(s) 936, 1266, 1721
Eam1105I GACNNNNNGTC 1 cut(s) 1623
EarI CTCTTC 3 cut(s) 936, 1266, 1721
Eco130I CCWWGG 2 cut(s) 138, 542
Eco31I GGTCTC 3 cut(s) 744, 1523, 1640
Eco47I GGWCC 1 cut(s) 826
Eco57I CTGAAG 1 cut(s) 1488
Eco91I GGTNACC 1 cut(s) 616
EcoO65I GGTNACC 1 cut(s) 616
EcoT14I CCWWGG 2 cut(s) 138, 542
ErhI CCWWGG 2 cut(s) 138, 542
FaeI CATG 9 cut(s) 73, 142, 806, 833, 1037, 1123, 1208, 1477, 1602
FaqI GGGAC 2 cut(s) 686, 1007
FatI CATG 9 cut(s) 69, 138, 802, 829, 1033, 1119, 1204, 1473, 1598
FauI CCCGC 1 cut(s) 42
FauNDI CATATG 1 cut(s) 1113
FbaI TGATCA 1 cut(s) 910
FokI GGATG 7 cut(s) 713, 920, 1021, 1115, 1193, 1592, 1709
FspBI CTAG 1 cut(s) 1716
GlaI GCGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 914
HaeIII GGCC 3 cut(s) 223, 366, 420
HapII CCGG 5 cut(s) 205, 582, 614, 743, 841
HgaI GACGC 2 cut(s) 460, 1726
HhaI GCGC 1 cut(s) 532
Hin1I GRCGYC 2 cut(s) 554, 1737
Hin1II CATG 9 cut(s) 73, 142, 806, 833, 1037, 1123, 1208, 1477, 1602
Hin6I GCGC 1 cut(s) 530
HinP1I GCGC 1 cut(s) 530
HinfI GANTC 5 cut(s) 25, 49, 277, 475, 727
HpaII CCGG 5 cut(s) 205, 582, 614, 743, 841
HphI GGTGA 6 cut(s) 628, 1220, 1328, 1411, 1462, 1642
Hpy166II GTNNAC 1 cut(s) 1453
Hpy188I TCNGA 8 cut(s) 54, 450, 492, 1078, 1102, 1333, 1563, 1680
Hpy188III TCNNGA 5 cut(s) 847, 914, 973, 1019, 1161
Hpy8I GTNNAC 1 cut(s) 1453
Hpy99I CGWCG 6 cut(s) 254, 454, 556, 559, 658, 1742
HpyAV CCTTC 8 cut(s) 279, 292, 301, 676, 952, 1293, 1330, 1456
HpyCH4III ACNGT 6 cut(s) 18, 179, 246, 468, 869, 1169
HpyCH4IV ACGT 3 cut(s) 339, 554, 1282
HpyCH4V TGCA 8 cut(s) 1204, 1382, 1441, 1488, 1571, 1602, 1672, 1688
HpyF3I CTNAG 8 cut(s) 53, 96, 186, 356, 410, 573, 930, 1392
HpySE526I ACGT 3 cut(s) 339, 554, 1282
Hsp92I GRCGYC 2 cut(s) 554, 1737
Hsp92II CATG 9 cut(s) 73, 142, 806, 833, 1037, 1123, 1208, 1477, 1602
HspAI GCGC 1 cut(s) 530
Ksp22I TGATCA 1 cut(s) 910
Kzo9I GATC 1 cut(s) 910
LguI GCTCTTC 1 cut(s) 1721
LmnI GCTCC 4 cut(s) 127, 207, 636, 799
Lsp1109I GCAGC 8 cut(s) 130, 133, 169, 241, 244, 247, 250, 438
LweI GCATC 2 cut(s) 1542, 1659
MaeI CTAG 1 cut(s) 1716
MaeII ACGT 3 cut(s) 339, 554, 1282
MaeIII GTNAC 5 cut(s) 12, 462, 468, 616, 1742
MalI GATC 1 cut(s) 912
MbiI CCGCTC 1 cut(s) 360
MboI GATC 1 cut(s) 910
MhlI GDGCHC 2 cut(s) 633, 1110
MlsI TGGCCA 2 cut(s) 366, 420
MluNI TGGCCA 2 cut(s) 366, 420
MlyI GAGTC 1 cut(s) 58
MmeI TCCRAC 5 cut(s) 473, 804, 915, 1056, 1080
Mox20I TGGCCA 2 cut(s) 366, 420
MscI TGGCCA 2 cut(s) 366, 420
MseI TTAA 4 cut(s) 456, 516, 1050, 1220
MslI CAYNNNNRTG 1 cut(s) 1142
Msp20I TGGCCA 2 cut(s) 366, 420
MspA1I CMGCKG 1 cut(s) 429
MspI CCGG 5 cut(s) 205, 582, 614, 743, 841
MspR9I CCNGG 1 cut(s) 744
Mva1269I GAATGC 2 cut(s) 1573, 1690
NciI CCSGG 1 cut(s) 744
NcoI CCATGG 1 cut(s) 138
NdeI CATATG 1 cut(s) 1113
NdeII GATC 1 cut(s) 910
NheI GCTAGC 1 cut(s) 1715
NlaIII CATG 9 cut(s) 73, 142, 806, 833, 1037, 1123, 1208, 1477, 1602
NlaIV GGNNCC 2 cut(s) 123, 1031
NmuCI GTSAC 4 cut(s) 12, 468, 616, 1742
NspI RCATGY 1 cut(s) 806
PciSI GCTCTTC 1 cut(s) 1721
PctI GAATGC 2 cut(s) 1573, 1690
PfeI GAWTC 4 cut(s) 25, 277, 475, 727
PflMI CCANNNNNTGG 1 cut(s) 1039
PleI GAGTC 1 cut(s) 57
PpsI GAGTC 1 cut(s) 57
PsiI TTATAA 1 cut(s) 1364
Psp1406I AACGTT 1 cut(s) 339
PspEI GGTNACC 1 cut(s) 616
PspN4I GGNNCC 2 cut(s) 123, 1031
PspPI GGNCC 1 cut(s) 826
PsrI GAACNNNNNNTAC 2 cut(s) 194, 226
RseI CAYNNNNRTG 1 cut(s) 1142
SapI GCTCTTC 1 cut(s) 1721
SaqAI TTAA 4 cut(s) 456, 516, 1050, 1220
Sau3AI GATC 1 cut(s) 910
Sau96I GGNCC 1 cut(s) 826
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 1 cut(s) 744
SduI GDGCHC 2 cut(s) 633, 1110
SfaNI GCATC 2 cut(s) 1542, 1659
SinI GGWCC 1 cut(s) 826
SmiMI CAYNNNNRTG 1 cut(s) 1142
SmlI CTYRAG 4 cut(s) 63, 132, 997, 1019
SmoI CTYRAG 4 cut(s) 63, 132, 997, 1019
SspMI CTAG 1 cut(s) 1716
StyD4I CCNGG 1 cut(s) 742
StyI CCWWGG 2 cut(s) 138, 542
TaaI ACNGT 6 cut(s) 18, 179, 246, 468, 869, 1169
TaiI ACGT 3 cut(s) 342, 557, 1285
TaqI TCGA 7 cut(s) 275, 478, 656, 713, 779, 846, 1187
TaqII GACCGA 2 cut(s) 726, 1634
TfiI GAWTC 4 cut(s) 25, 277, 475, 727
Tru1I TTAA 4 cut(s) 456, 516, 1050, 1220
Tru9I TTAA 4 cut(s) 456, 516, 1050, 1220
TscAI CASTG 1 cut(s) 874
TseFI GTSAC 4 cut(s) 12, 468, 616, 1742
TseI GCWGC 8 cut(s) 143, 146, 157, 229, 232, 235, 238, 426
Tsp45I GTSAC 4 cut(s) 12, 468, 616, 1742
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 1 cut(s) 874
Van91I CCANNNNNTGG 1 cut(s) 1039
VpaK11BI GGWCC 1 cut(s) 826
XapI RAATTY 3 cut(s) 494, 946, 1216
XceI RCATGY 1 cut(s) 806
XspI CTAG 1 cut(s) 1716
ZraI GACGTC 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.