Rh5BG546900

PRLI-interacting factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007381
Physical Location & Seq
Forward (+)
86904150 .. 86908387
4238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG546900.1

Sequence Viewer

Length: 1836 bp
ATGAACGATAAGTCACAGTTGATGAATCAACCCCCACCGCCGCCGATGATGAGTCTGAGCCAAGCTCAAGCCATGAGCCAGCCGCCGCCGATGATGGCTCAGCCGCCGCCGCCGCAGATGATGGCTCCGTCTTCTCAAGCCATGGCTGCTGCTGCTGCTGCTCAGCAGCAAGTACAGATGATGATGAACAGTCAATCTCAGCCGTTGATGATGAACCGGAGCTACAAGGCTTGGCCGCCGCAGCAGCAGCAGCAGCAGCAACAGTTCGTCGCCACCCAGAAGCCGCCGCAGTCGAATCTGGGAAGGAACAATTGGAAGGGTAAGAAGGGAAATGACAAGCGGAGAATGGAGAAAAACGTTCCCAATTTGCCCCTGAGCGGTGGCCAGAGCAGTAGCGGAGGAGGGTATAATGTTCCCAATTTGCCCCTGAGTGGTGGCCAGAGCAGTAGCGGAGGAGGGTATAATGTTCCCAATTTGCCCCTGAGTGGTGGCCAGAGCAGCGGAGGAGGCGGAGGAGGAGGAGGGTATATACCTCCGACGCTTAACGAGTTACAGTCACAGAATCGAATGAAAGCTCGGAAATTTTACCCCAAAAAGAAGTTTAACAATAGGTTTGCGCCTTACGCCCCAAGGAACACGACGTCGTTTATCATTAGAGCTAAGAAGTCCGGTGGGATTGCTTCGCTGGTTTCGCCTTGTCCGGTGACCCCTGCCGTGCTCCCCACGCCCATTTTCTCGCCGTCGAGGGAGGTTTTGGGGGACACGGCGAAGGAGGAGTGGGGTGTGGATGGGTATGGGTCGATGAAAGGGTTGATTCGGCTTCGGTCTCCCGGGAATGATGATGATGAGGAGGGAGGGGGTGGGTCGAGTGAGAGTGATGTGGAGGAGCATGTGGAGGTTGAGAGGAGGTTGGACCATGATCTGAGCCGGTTCGAGATGATATACCCGAATTACAGTGGGGTTGAGTATAATAATGTGCTGGAGAATAGAGTGGATGATCAGGATACACACATTGCTCAGTTGGAGGAAGAGAATTTGACTTTGAAGGAGAGGCTGTTCTTGATGGAGAGGGAGTTGGGGGACTTGAGGAGGAGGATGTTGTTTCTTGAGAGGAGGCACCATGTGATGGAGGAGGTTAATGAGGAGGTTGTGGAGAATGGGTCGGAGAACGATAGTGATGGAGGGTCGGATGTGCCCCATATGGGCATAGGGGGAGAGAGCAACATTGAGGTGGTAGATTATGTGTCCAGAAACGGTGGGTTTGGGGATGTTTCGATGGAAGAATATGTGCATGGTGAAATGAATTTAAAGGAAAATGAGGTCAAAGATGAAGTGAGAAATGAGGTTGTAGGTAATGAAGAGATTGAACGTAAGAACGAGGGAAGAAGGAATGACCTTGTAGGTGATGGGGTCGTTTCAGAGAAGGTTGAGGAAGTGAGGAATGAGTTTATAATGAACAAAGTAATTGCAAATAAAACTGAGCTGGTGAGTAGTGAAGTTGAGACAAAGAATGAGAATGAGTTTATGCAGGGTCAGGTGAACATAGAGAAGGCTGAAGACATGGTAATTGCCTGCAATGAGAAGAATGAAACTAAAGTTATGAGAAATGAAGTTGAGACCAAGCTTGAAAATGAGTTGATGCGCGGTCAGATGAATGCAGAGAAGGATGAAGAAATGGGGACTTCATGCACAGAGAATAATAAGACCGAGGGTCAGGTGAAGAATGAAGTTGAGACCAAGAATGAGAGTGAGTTGATGCAGGGTCAGATGAATGCACAGAGGGATGAAGAAATGGGAATTGGCAGCACAGAGAAGAGCATTGGCGTCGGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

611

Amino Acids

67.66

Weight (kDa)

4.86

Isoelectric Point (pI)

63.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1451
AatII GACGTC 1 cut(s) 644
AccB1I GGYRCC 1 cut(s) 1116
AccB7I CCANNNNNTGG 1 cut(s) 1126
AccBSI CCGCTC 1 cut(s) 378
AccII CGCG 1 cut(s) 1644
AclI AACGTT 1 cut(s) 357
AcoI YGGCCR 4 cut(s) 233, 382, 436, 490
AcsI RAATTY 3 cut(s) 581, 1033, 1303
AcuI CTGAAG 1 cut(s) 1575
AcyI GRCGYC 2 cut(s) 641, 1824
AdeI CACNNNGTG 1 cut(s) 1123
AfaI GTAC 1 cut(s) 174
AfiI CCNNNNNNNGG 5 cut(s) 377, 431, 485, 1126, 1202
AgsI TTSAA 3 cut(s) 1045, 1367, 1628
AhdI GACNNNNNGTC 1 cut(s) 1710
AjuI GAANNNNNNNTTGG 4 cut(s) 295, 327, 1782, 1814
AloI GAACNNNNNNTCC 2 cut(s) 1040, 1072
AluBI AGCT 6 cut(s) 65, 222, 575, 659, 1483, 1624
AluI AGCT 6 cut(s) 65, 222, 575, 659, 1483, 1624
Alw21I GWGCWC 1 cut(s) 720
Alw26I GTCTC 4 cut(s) 831, 1496, 1610, 1727
Ama87I CYCGRG 1 cut(s) 830
AoxI GGCC 4 cut(s) 233, 382, 436, 490
ApoI RAATTY 3 cut(s) 581, 1033, 1303
AspLEI GCGC 2 cut(s) 619, 1644
AspS9I GGNCC 1 cut(s) 913
AsuC2I CCSGG 2 cut(s) 831, 832
AsuHPI GGTGA 6 cut(s) 715, 1307, 1415, 1498, 1549, 1729
AvaI CYCGRG 1 cut(s) 830
AvaII GGWCC 1 cut(s) 913
BaeGI GKGCMC 1 cut(s) 1197
BalI TGGCCA 3 cut(s) 384, 438, 492
BanI GGYRCC 1 cut(s) 1116
BbsI GAAGAC 2 cut(s) 123, 1563
Bbv12I GWGCWC 1 cut(s) 720
BccI CCATC 8 cut(s) 88, 115, 782, 1057, 1120, 1172, 1270, 1400
BceAI ACGGC 4 cut(s) 187, 698, 724, 780
BcgI CGANNNNNNTGC 1 cut(s) 1807
BciVI GTATCC 1 cut(s) 997
BclI TGATCA 1 cut(s) 997
BcnI CCSGG 2 cut(s) 831, 832
BcoDI GTCTC 4 cut(s) 831, 1496, 1610, 1727
BfuI GTATCC 1 cut(s) 997
BlpI GCTNAGC 2 cut(s) 99, 162
Bme1390I CCNGG 2 cut(s) 831, 832
Bme18I GGWCC 1 cut(s) 913
BmeRI GACNNNNNGTC 1 cut(s) 1710
BmeT110I CYCGRG 1 cut(s) 830
BmgT120I GGNCC 1 cut(s) 913
BmiI GGNNCC 2 cut(s) 126, 1118
BmrFI CCNGG 2 cut(s) 831, 832
BmsI GCATC 2 cut(s) 1629, 1746
BpiI GAAGAC 2 cut(s) 123, 1563
BplI GAGNNNNNCTC 2 cut(s) 49, 81
BpmI CTGGAG 1 cut(s) 1001
Bpu10I CCTNAGC 1 cut(s) 374
Bpu1102I GCTNAGC 2 cut(s) 99, 162
BpuEI CTTGAG 4 cut(s) 51, 120, 1105, 1127
BpuMI CCSGG 2 cut(s) 831, 832
BsaHI GRCGYC 2 cut(s) 641, 1824
BsaI GGTCTC 3 cut(s) 831, 1610, 1727
BsaJI CCNNGG 4 cut(s) 141, 629, 830, 1707
BsaWI WCCGGW 3 cut(s) 216, 668, 700
BsaXI ACNNNNNCTCC 6 cut(s) 1040, 1058, 1070, 1088, 1145, 1175
Bsc4I CCNNNNNNNGG 5 cut(s) 377, 431, 485, 1126, 1202
Bse118I RCCGGY 1 cut(s) 927
Bse3DI GCAATG 2 cut(s) 1011, 1582
BseDI CCNNGG 4 cut(s) 141, 629, 830, 1707
BseGI GGATG 7 cut(s) 793, 1000, 1101, 1195, 1273, 1672, 1789
BseLI CCNNNNNNNGG 5 cut(s) 377, 431, 485, 1126, 1202
BseMI GCAATG 2 cut(s) 1011, 1582
BseSI GKGCMC 1 cut(s) 1197
Bsh1236I CGCG 1 cut(s) 1644
BshFI GGCC 4 cut(s) 235, 384, 438, 492
BshNI GGYRCC 1 cut(s) 1116
BsiHKAI GWGCWC 1 cut(s) 720
BsiHKCI CYCGRG 1 cut(s) 830
BsiSI CCGG 5 cut(s) 217, 669, 701, 831, 928
BslFI GGGAC 3 cut(s) 773, 1094, 1693
BslI CCNNNNNNNGG 5 cut(s) 377, 431, 485, 1126, 1202
BsmAI GTCTC 4 cut(s) 831, 1496, 1610, 1727
BsmFI GGGAC 3 cut(s) 773, 1094, 1693
BsmI GAATGC 2 cut(s) 1660, 1777
BsnI GGCC 4 cut(s) 235, 384, 438, 492
Bso31I GGTCTC 3 cut(s) 831, 1610, 1727
BsoBI CYCGRG 1 cut(s) 830
Bsp1286I GDGCHC 2 cut(s) 720, 1197
Bsp143I GATC 2 cut(s) 919, 997
Bsp1720I GCTNAGC 2 cut(s) 99, 162
Bsp19I CCATGG 1 cut(s) 141
BspANI GGCC 4 cut(s) 235, 384, 438, 492
BspFNI CGCG 1 cut(s) 1644
BspLI GGNNCC 2 cut(s) 126, 1118
BspQI GCTCTTC 1 cut(s) 1808
BspT107I GGYRCC 1 cut(s) 1116
BspTNI GGTCTC 3 cut(s) 831, 1610, 1727
BsrBI CCGCTC 1 cut(s) 378
BsrDI GCAATG 2 cut(s) 1011, 1582
BsrFI RCCGGY 1 cut(s) 927
BssAI RCCGGY 1 cut(s) 927
BssECI CCNNGG 4 cut(s) 141, 629, 830, 1707
BssMI GATC 2 cut(s) 919, 997
BssNI GRCGYC 2 cut(s) 641, 1824
BssT1I CCWWGG 2 cut(s) 141, 629
Bst4CI ACNGT 6 cut(s) 18, 191, 264, 555, 956, 1256
Bst6I CTCTTC 3 cut(s) 1023, 1353, 1808
BstACI GRCGYC 2 cut(s) 641, 1824
BstC8I GCNNGC 2 cut(s) 80, 1573
BstDSI CCRYGG 1 cut(s) 141
BstEII GGTNACC 1 cut(s) 703
BstF5I GGATG 7 cut(s) 793, 1000, 1101, 1195, 1273, 1672, 1789
BstFNI CGCG 1 cut(s) 1644
BstHHI GCGC 2 cut(s) 619, 1644
BstKTI GATC 2 cut(s) 922, 1000
BstMAI GTCTC 4 cut(s) 831, 1496, 1610, 1727
BstMBI GATC 2 cut(s) 919, 997
BstNSI RCATGY 1 cut(s) 893
BstPI GGTNACC 1 cut(s) 703
BstSCI CCNGG 2 cut(s) 829, 830
BstSLI GKGCMC 1 cut(s) 1197
BstUI CGCG 1 cut(s) 1644
BstV2I GAAGAC 2 cut(s) 123, 1563
BsuI GTATCC 1 cut(s) 997
BsuRI GGCC 4 cut(s) 235, 384, 438, 492
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 7 cut(s) 793, 1000, 1101, 1195, 1273, 1672, 1789
BtsIMutI CAGTG 1 cut(s) 961
Cac8I GCNNGC 2 cut(s) 80, 1573
CfoI GCGC 2 cut(s) 619, 1644
Cfr10I RCCGGY 1 cut(s) 927
Cfr13I GGNCC 1 cut(s) 913
Cfr9I CCCGGG 1 cut(s) 830
CseI GACGC 2 cut(s) 547, 1813
Csp6I GTAC 1 cut(s) 173
CviAII CATG 8 cut(s) 73, 142, 890, 917, 1121, 1292, 1561, 1686
CviQI GTAC 1 cut(s) 173
DpnI GATC 2 cut(s) 921, 999
DpnII GATC 2 cut(s) 919, 997
DraI TTTAAA 1 cut(s) 1308
DraIII CACNNNGTG 1 cut(s) 1123
DriI GACNNNNNGTC 1 cut(s) 1710
EaeI YGGCCR 4 cut(s) 233, 382, 436, 490
Eam1104I CTCTTC 3 cut(s) 1023, 1353, 1808
Eam1105I GACNNNNNGTC 1 cut(s) 1710
EarI CTCTTC 3 cut(s) 1023, 1353, 1808
EciI GGCGGA 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 141, 629
Eco31I GGTCTC 3 cut(s) 831, 1610, 1727
Eco47I GGWCC 1 cut(s) 913
Eco57I CTGAAG 1 cut(s) 1575
Eco88I CYCGRG 1 cut(s) 830
Eco91I GGTNACC 1 cut(s) 703
EcoO65I GGTNACC 1 cut(s) 703
EcoT14I CCWWGG 2 cut(s) 141, 629
ErhI CCWWGG 2 cut(s) 141, 629
FaeI CATG 8 cut(s) 76, 145, 893, 920, 1124, 1295, 1564, 1689
FaqI GGGAC 3 cut(s) 773, 1094, 1693
FatI CATG 8 cut(s) 72, 141, 889, 916, 1120, 1291, 1560, 1685
FauNDI CATATG 1 cut(s) 1200
FbaI TGATCA 1 cut(s) 997
FokI GGATG 7 cut(s) 800, 1007, 1108, 1202, 1280, 1679, 1796
GlaI GCGC 2 cut(s) 618, 1643
GsuI CTGGAG 1 cut(s) 1001
HaeIII GGCC 4 cut(s) 235, 384, 438, 492
HapII CCGG 5 cut(s) 217, 669, 701, 831, 928
HgaI GACGC 2 cut(s) 547, 1813
HhaI GCGC 2 cut(s) 619, 1644
Hin1I GRCGYC 2 cut(s) 641, 1824
Hin1II CATG 8 cut(s) 76, 145, 893, 920, 1124, 1295, 1564, 1689
Hin6I GCGC 2 cut(s) 617, 1642
HinP1I GCGC 2 cut(s) 617, 1642
HindIII AAGCTT 1 cut(s) 1622
HinfI GANTC 5 cut(s) 25, 52, 295, 562, 814
HpaII CCGG 5 cut(s) 217, 669, 701, 831, 928
HphI GGTGA 6 cut(s) 715, 1307, 1415, 1498, 1549, 1729
Hpy166II GTNNAC 1 cut(s) 1540
Hpy188I TCNGA 9 cut(s) 57, 537, 579, 924, 1165, 1189, 1420, 1650, 1767
Hpy188III TCNNGA 5 cut(s) 934, 1001, 1060, 1106, 1248
Hpy8I GTNNAC 1 cut(s) 1540
Hpy99I CGWCG 6 cut(s) 272, 541, 643, 646, 745, 1829
HpyAV CCTTC 9 cut(s) 297, 310, 319, 763, 1039, 1380, 1417, 1543, 1657
HpyCH4III ACNGT 6 cut(s) 18, 191, 264, 555, 956, 1256
HpyCH4IV ACGT 3 cut(s) 357, 641, 1369
HpyCH4V TGCA 8 cut(s) 1291, 1469, 1528, 1575, 1658, 1689, 1759, 1775
HpySE526I ACGT 3 cut(s) 357, 641, 1369
Hsp92I GRCGYC 2 cut(s) 641, 1824
Hsp92II CATG 8 cut(s) 76, 145, 893, 920, 1124, 1295, 1564, 1689
HspAI GCGC 2 cut(s) 617, 1642
Ksp22I TGATCA 1 cut(s) 997
Kzo9I GATC 2 cut(s) 919, 997
LguI GCTCTTC 1 cut(s) 1808
LmnI GCTCC 4 cut(s) 130, 219, 723, 886
LweI GCATC 2 cut(s) 1629, 1746
MaeII ACGT 3 cut(s) 357, 641, 1369
MaeIII GTNAC 5 cut(s) 12, 549, 555, 703, 1829
MalI GATC 2 cut(s) 921, 999
MbiI CCGCTC 1 cut(s) 378
MboI GATC 2 cut(s) 919, 997
MfeI CAATTG 1 cut(s) 310
MhlI GDGCHC 2 cut(s) 720, 1197
MlsI TGGCCA 3 cut(s) 384, 438, 492
MluNI TGGCCA 3 cut(s) 384, 438, 492
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 5 cut(s) 560, 891, 1002, 1143, 1167
Mox20I TGGCCA 3 cut(s) 384, 438, 492
MscI TGGCCA 3 cut(s) 384, 438, 492
MseI TTAA 4 cut(s) 543, 603, 1137, 1307
MslI CAYNNNNRTG 1 cut(s) 1229
Msp20I TGGCCA 3 cut(s) 384, 438, 492
MspA1I CMGCKG 1 cut(s) 501
MspI CCGG 5 cut(s) 217, 669, 701, 831, 928
MspR9I CCNGG 2 cut(s) 831, 832
MunI CAATTG 1 cut(s) 310
Mva1269I GAATGC 2 cut(s) 1660, 1777
MvnI CGCG 1 cut(s) 1644
NciI CCSGG 2 cut(s) 831, 832
NcoI CCATGG 1 cut(s) 141
NdeI CATATG 1 cut(s) 1200
NdeII GATC 2 cut(s) 919, 997
NlaIII CATG 8 cut(s) 76, 145, 893, 920, 1124, 1295, 1564, 1689
NlaIV GGNNCC 2 cut(s) 126, 1118
NmuCI GTSAC 4 cut(s) 12, 555, 703, 1829
NspI RCATGY 1 cut(s) 893
PciSI GCTCTTC 1 cut(s) 1808
PctI GAATGC 2 cut(s) 1660, 1777
PfeI GAWTC 4 cut(s) 25, 295, 562, 814
PflMI CCANNNNNTGG 1 cut(s) 1126
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
PsiI TTATAA 1 cut(s) 1451
Psp1406I AACGTT 1 cut(s) 357
PspEI GGTNACC 1 cut(s) 703
PspN4I GGNNCC 2 cut(s) 126, 1118
PspPI GGNCC 1 cut(s) 913
PsrI GAACNNNNNNTAC 2 cut(s) 206, 238
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
RseI CAYNNNNRTG 1 cut(s) 1229
SapI GCTCTTC 1 cut(s) 1808
SaqAI TTAA 4 cut(s) 543, 603, 1137, 1307
Sau3AI GATC 2 cut(s) 919, 997
Sau96I GGNCC 1 cut(s) 913
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 2 cut(s) 831, 832
SduI GDGCHC 2 cut(s) 720, 1197
SfaNI GCATC 2 cut(s) 1629, 1746
SinI GGWCC 1 cut(s) 913
SmaI CCCGGG 1 cut(s) 832
SmiMI CAYNNNNRTG 1 cut(s) 1229
SmlI CTYRAG 4 cut(s) 66, 135, 1084, 1106
SmoI CTYRAG 4 cut(s) 66, 135, 1084, 1106
StyD4I CCNGG 2 cut(s) 829, 830
StyI CCWWGG 2 cut(s) 141, 629
TaaI ACNGT 6 cut(s) 18, 191, 264, 555, 956, 1256
TaiI ACGT 3 cut(s) 360, 644, 1372
TaqI TCGA 7 cut(s) 293, 565, 743, 800, 866, 933, 1274
TaqII GACCGA 2 cut(s) 813, 1721
TatI WGTACW 1 cut(s) 172
TfiI GAWTC 4 cut(s) 25, 295, 562, 814
Tru1I TTAA 4 cut(s) 543, 603, 1137, 1307
Tru9I TTAA 4 cut(s) 543, 603, 1137, 1307
TscAI CASTG 1 cut(s) 961
TseFI GTSAC 4 cut(s) 12, 555, 703, 1829
Tsp45I GTSAC 4 cut(s) 12, 555, 703, 1829
TspGWI ACGGA 1 cut(s) 117
TspMI CCCGGG 1 cut(s) 830
TspRI CASTG 1 cut(s) 961
Van91I CCANNNNNTGG 1 cut(s) 1126
VpaK11BI GGWCC 1 cut(s) 913
XapI RAATTY 3 cut(s) 581, 1033, 1303
XceI RCATGY 1 cut(s) 893
XmaI CCCGGG 1 cut(s) 830
ZraI GACGTC 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.