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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G64570 |
| fragaria_vesca | FvH4_3g02070 |
| malus_domestica | MD05G1346800.v1.1 MD05G1347100.v1.1 MD05G1347200.v1.1 MD10G1321400.v1.1 |
| prunus_persica | Prupe.4G020900_v2.0.a1 Prupe.4G020900_v2.0.a1 Prupe.4G020900_v2.0.a1 |
| pyrus_communis | pycom05g31490 pycom10g27210 |
| rosa_chinensis | RchiOBHm_Chr5g0002971 RchiOBHm_Chr5g0003031 RchiOBHm_Chr5g0003091 |
| rosa_laevigata | RLG00000031075 |
| rosa_multiflora | Rmu_sc0000146.1_g000022 Rmu_sc0013603.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00072910 |
| rosa_rugosa | Rorug04G0400900 |
| rosa_samantha | Rh5AG024000 Rh5AG025000 Rh5BG026700 Rh5CG026800 Rh5CG027800 Rh5DG025300 |
| rosa_wichuraiana | Rw5G002270 Rw5G002320 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2064, 3507 |
| AarI | CACCTGC | 1 cut(s) | 2324 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 922 |
| AatII | GACGTC | 1 cut(s) | 506 |
| Acc16I | TGCGCA | 1 cut(s) | 1338 |
| Acc36I | ACCTGC | 2 cut(s) | 2324, 3811 |
| AccB1I | GGYRCC | 3 cut(s) | 1402, 1969, 2765 |
| AccBSI | CCGCTC | 1 cut(s) | 2144 |
| AciI | CCGC | 7 cut(s) | 562, 1803, 2022, 2142, 2194, 2197, 3555 |
| AclI | AACGTT | 1 cut(s) | 1903 |
| AclWI | GGATC | 4 cut(s) | 2105, 2577, 2590, 3745 |
| AcoI | YGGCCR | 1 cut(s) | 2079 |
| AcsI | RAATTY | 7 cut(s) | 668, 904, 1978, 2090, 2901, 3520, 3753 |
| AcuI | CTGAAG | 8 cut(s) | 228, 327, 384, 489, 2936, 3003, 3690, 3741 |
| AcvI | CACGTG | 1 cut(s) | 3161 |
| AcyI | GRCGYC | 1 cut(s) | 503 |
| AfaI | GTAC | 9 cut(s) | 662, 832, 1143, 1221, 1239, 1699, 2383, 2721, 3463 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 36, 2987, 3344, 3464, 4101, 4116 |
| AflIII | ACRYGT | 3 cut(s) | 296, 412, 1105 |
| AjiI | CACGTC | 1 cut(s) | 297 |
| AjnI | CCWGG | 9 cut(s) | 497, 1003, 1154, 1173, 1434, 1494, 2812, 3457, 4109 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1890, 1922 |
| AleI | CACNNNNGTG | 2 cut(s) | 1209, 1313 |
| Alw21I | GWGCWC | 4 cut(s) | 206, 1588, 2632, 3819 |
| Alw26I | GTCTC | 7 cut(s) | 9, 1393, 2368, 2698, 3308, 3627, 4009 |
| Alw44I | GTGCAC | 1 cut(s) | 2628 |
| AlwI | GGATC | 4 cut(s) | 2105, 2577, 2590, 3745 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3719 |
| AoxI | GGCC | 7 cut(s) | 558, 1078, 1417, 1746, 2079, 2355, 2810 |
| ApaI | GGGCCC | 1 cut(s) | 562 |
| ApaLI | GTGCAC | 1 cut(s) | 2628 |
| ApoI | RAATTY | 7 cut(s) | 668, 904, 1978, 2090, 2901, 3520, 3753 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 8, 40 |
| AseI | ATTAAT | 1 cut(s) | 2418 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 603, 789 |
| AspLEI | GCGC | 4 cut(s) | 1339, 2139, 2886, 4063 |
| AsuC2I | CCSGG | 1 cut(s) | 2379 |
| AsuII | TTCGAA | 1 cut(s) | 14 |
| AvaII | GGWCC | 6 cut(s) | 446, 959, 2318, 3467, 3800, 4079 |
| BaeGI | GKGCMC | 3 cut(s) | 562, 1972, 2632 |
| BamHI | GGATCC | 1 cut(s) | 2582 |
| BanI | GGYRCC | 3 cut(s) | 1402, 1969, 2765 |
| BanII | GRGCYC | 2 cut(s) | 206, 562 |
| BauI | CACGAG | 1 cut(s) | 988 |
| BbrPI | CACGTG | 1 cut(s) | 3161 |
| BbsI | GAAGAC | 4 cut(s) | 296, 1020, 1341, 2898 |
| Bbv12I | GWGCWC | 4 cut(s) | 206, 1588, 2632, 3819 |
| BbvCI | CCTCAGC | 2 cut(s) | 1624, 3066 |
| BceAI | ACGGC | 3 cut(s) | 1523, 2066, 2983 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1934, 1968, 3944, 3978 |
| BciT130I | CCWGG | 9 cut(s) | 499, 1005, 1156, 1175, 1436, 1496, 2814, 3459, 4111 |
| BciVI | GTATCC | 2 cut(s) | 2496, 3910 |
| BclI | TGATCA | 1 cut(s) | 3268 |
| BcnI | CCSGG | 1 cut(s) | 2379 |
| BcoDI | GTCTC | 7 cut(s) | 9, 1393, 2368, 2698, 3308, 3627, 4009 |
| BfmI | CTRYAG | 2 cut(s) | 3016, 4152 |
| BfuAI | ACCTGC | 2 cut(s) | 2324, 3811 |
| BfuI | GTATCC | 2 cut(s) | 2496, 3910 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2248 |
| BglII | AGATCT | 2 cut(s) | 514, 893 |
| BmcAI | AGTACT | 1 cut(s) | 1221 |
| Bme18I | GGWCC | 6 cut(s) | 446, 959, 2318, 3467, 3800, 4079 |
| BmgBI | CACGTC | 1 cut(s) | 297 |
| BmrI | ACTGGG | 1 cut(s) | 2581 |
| BmuI | ACTGGG | 1 cut(s) | 2581 |
| BpiI | GAAGAC | 4 cut(s) | 296, 1020, 1341, 2898 |
| BpmI | CTGGAG | 3 cut(s) | 1138, 2772, 4147 |
| Bpu10I | CCTNAGC | 3 cut(s) | 571, 1624, 3066 |
| Bpu14I | TTCGAA | 1 cut(s) | 14 |
| BpuEI | CTTGAG | 5 cut(s) | 421, 1572, 2039, 2554, 2784 |
| BpuMI | CCSGG | 1 cut(s) | 2379 |
| BsaAI | YACGTR | 2 cut(s) | 2723, 3161 |
| BsaBI | GATNNNNATC | 4 cut(s) | 153, 456, 3174, 3423 |
| BsaHI | GRCGYC | 1 cut(s) | 503 |
| BsaI | GGTCTC | 1 cut(s) | 2368 |
| BsaJI | CCNNGG | 7 cut(s) | 1004, 1173, 1495, 1727, 2126, 2322, 4109 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2365, 2395 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 36, 2987, 3344, 3464, 4101, 4116 |
| Bse3DI | GCAATG | 1 cut(s) | 926 |
| Bse8I | GATNNNNATC | 4 cut(s) | 153, 456, 3174, 3423 |
| BseBI | CCWGG | 9 cut(s) | 499, 1005, 1156, 1175, 1436, 1496, 2814, 3459, 4111 |
| BseDI | CCNNGG | 7 cut(s) | 1004, 1173, 1495, 1727, 2126, 2322, 4109 |
| BseJI | GATNNNNATC | 4 cut(s) | 153, 456, 3174, 3423 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 36, 2987, 3344, 3464, 4101, 4116 |
| BseMI | GCAATG | 1 cut(s) | 926 |
| BseRI | GAGGAG | 7 cut(s) | 128, 149, 767, 2427, 2946, 3719, 3953 |
| BseSI | GKGCMC | 3 cut(s) | 562, 1972, 2632 |
| BsgI | GTGCAG | 4 cut(s) | 1130, 2331, 3219, 3438 |
| BshFI | GGCC | 7 cut(s) | 560, 1080, 1419, 1748, 2081, 2357, 2812 |
| BshNI | GGYRCC | 3 cut(s) | 1402, 1969, 2765 |
| BsiHKAI | GWGCWC | 4 cut(s) | 206, 1588, 2632, 3819 |
| BsiSI | CCGG | 3 cut(s) | 30, 182, 2378 |
| BslFI | GGGAC | 6 cut(s) | 459, 918, 1709, 2064, 2084, 2304 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 36, 2987, 3344, 3464, 4101, 4116 |
| BsmAI | GTCTC | 7 cut(s) | 9, 1393, 2368, 2698, 3308, 3627, 4009 |
| BsmFI | GGGAC | 6 cut(s) | 459, 918, 1709, 2064, 2084, 2304 |
| BsmI | GAATGC | 1 cut(s) | 2785 |
| BsnI | GGCC | 7 cut(s) | 560, 1080, 1419, 1748, 2081, 2357, 2812 |
| Bso31I | GGTCTC | 1 cut(s) | 2368 |
| Bsp119I | TTCGAA | 1 cut(s) | 14 |
| Bsp120I | GGGCCC | 1 cut(s) | 558 |
| Bsp1286I | GDGCHC | 6 cut(s) | 206, 562, 1588, 1972, 2632, 3819 |
| Bsp19I | CCATGG | 1 cut(s) | 2322 |
| BspACI | CCGC | 7 cut(s) | 562, 1803, 2022, 2142, 2194, 2197, 3555 |
| BspANI | GGCC | 7 cut(s) | 560, 1080, 1419, 1748, 2081, 2357, 2812 |
| BspHI | TCATGA | 1 cut(s) | 2350 |
| BspMAI | CTGCAG | 1 cut(s) | 3020 |
| BspMI | ACCTGC | 2 cut(s) | 2324, 3811 |
| BspPI | GGATC | 4 cut(s) | 2105, 2577, 2590, 3745 |
| BspQI | GCTCTTC | 2 cut(s) | 780, 2212 |
| BspT104I | TTCGAA | 1 cut(s) | 14 |
| BspT107I | GGYRCC | 3 cut(s) | 1402, 1969, 2765 |
| BspTNI | GGTCTC | 1 cut(s) | 2368 |
| BsrBI | CCGCTC | 1 cut(s) | 2144 |
| BsrDI | GCAATG | 1 cut(s) | 926 |
| BssECI | CCNNGG | 7 cut(s) | 1004, 1173, 1495, 1727, 2126, 2322, 4109 |
| BssNI | GRCGYC | 1 cut(s) | 503 |
| BssSI | CACGAG | 1 cut(s) | 988 |
| BssT1I | CCWWGG | 2 cut(s) | 1727, 2322 |
| Bst2BI | CACGAG | 1 cut(s) | 988 |
| Bst2UI | CCWGG | 9 cut(s) | 499, 1005, 1156, 1175, 1436, 1496, 2814, 3459, 4111 |
| Bst4CI | ACNGT | 8 cut(s) | 227, 835, 1598, 2276, 2411, 3044, 3217, 4153 |
| Bst6I | CTCTTC | 6 cut(s) | 114, 651, 780, 2022, 2212, 3645 |
| BstACI | GRCGYC | 1 cut(s) | 503 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 2537, 2965 |
| BstBAI | YACGTR | 2 cut(s) | 2723, 3161 |
| BstBI | TTCGAA | 1 cut(s) | 14 |
| BstC8I | GCNNGC | 2 cut(s) | 562, 1341 |
| BstDSI | CCRYGG | 1 cut(s) | 2322 |
| BstEII | GGTNACC | 1 cut(s) | 3614 |
| BstHHI | GCGC | 4 cut(s) | 1339, 2139, 2886, 4063 |
| BstMAI | GTCTC | 7 cut(s) | 9, 1393, 2368, 2698, 3308, 3627, 4009 |
| BstNI | CCWGG | 9 cut(s) | 499, 1005, 1156, 1175, 1436, 1496, 2814, 3459, 4111 |
| BstNSI | RCATGY | 5 cut(s) | 416, 1109, 1343, 3228, 3747 |
| BstPI | GGTNACC | 1 cut(s) | 3614 |
| BstSFI | CTRYAG | 2 cut(s) | 3016, 4152 |
| BstSLI | GKGCMC | 3 cut(s) | 562, 1972, 2632 |
| BstV2I | GAAGAC | 4 cut(s) | 296, 1020, 1341, 2898 |
| BstX2I | RGATCY | 4 cut(s) | 514, 893, 2110, 2582 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2744 |
| BstYI | RGATCY | 4 cut(s) | 514, 893, 2110, 2582 |
| BsuI | GTATCC | 2 cut(s) | 2496, 3910 |
| BsuRI | GGCC | 7 cut(s) | 560, 1080, 1419, 1748, 2081, 2357, 2812 |
| BtgI | CCRYGG | 1 cut(s) | 2322 |
| BtrI | CACGTC | 1 cut(s) | 297 |
| BtsI | GCAGTG | 4 cut(s) | 675, 1062, 2138, 4174 |
| BveI | ACCTGC | 2 cut(s) | 2324, 3811 |
| Cac8I | GCNNGC | 2 cut(s) | 562, 1341 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3719 |
| CciI | TCATGA | 1 cut(s) | 2350 |
| CfoI | GCGC | 4 cut(s) | 1339, 2139, 2886, 4063 |
| CseI | GACGC | 3 cut(s) | 920, 1335, 3199 |
| CsiI | ACCWGGT | 1 cut(s) | 3457 |
| Csp6I | GTAC | 9 cut(s) | 661, 831, 1142, 1220, 1238, 1698, 2382, 2720, 3462 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1437, 1472 |
| CviQI | GTAC | 9 cut(s) | 661, 831, 1142, 1220, 1238, 1698, 2382, 2720, 3462 |
| DraI | TTTAAA | 2 cut(s) | 2211, 2710 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 922 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 922 |
| EaeI | YGGCCR | 1 cut(s) | 2079 |
| Eam1104I | CTCTTC | 6 cut(s) | 114, 651, 780, 2022, 2212, 3645 |
| EarI | CTCTTC | 6 cut(s) | 114, 651, 780, 2022, 2212, 3645 |
| Ecl136II | GAGCTC | 1 cut(s) | 204 |
| Eco130I | CCWWGG | 2 cut(s) | 1727, 2322 |
| Eco147I | AGGCCT | 2 cut(s) | 2357, 2812 |
| Eco24I | GRGCYC | 2 cut(s) | 206, 562 |
| Eco31I | GGTCTC | 1 cut(s) | 2368 |
| Eco32I | GATATC | 1 cut(s) | 2034 |
| Eco47I | GGWCC | 6 cut(s) | 446, 959, 2318, 3467, 3800, 4079 |
| Eco53kI | GAGCTC | 1 cut(s) | 204 |
| Eco57I | CTGAAG | 8 cut(s) | 228, 327, 384, 489, 2936, 3003, 3690, 3741 |
| Eco72I | CACGTG | 1 cut(s) | 3161 |
| Eco91I | GGTNACC | 1 cut(s) | 3614 |
| EcoICRI | GAGCTC | 1 cut(s) | 204 |
| EcoO109I | RGGNCCY | 3 cut(s) | 446, 3800, 4079 |
| EcoO65I | GGTNACC | 1 cut(s) | 3614 |
| EcoRII | CCWGG | 9 cut(s) | 497, 1003, 1154, 1173, 1434, 1494, 2812, 3457, 4109 |
| EcoRV | GATATC | 1 cut(s) | 2034 |
| EcoT14I | CCWWGG | 2 cut(s) | 1727, 2322 |
| EcoT22I | ATGCAT | 1 cut(s) | 2961 |
| EcoT38I | GRGCYC | 2 cut(s) | 206, 562 |
| ErhI | CCWWGG | 2 cut(s) | 1727, 2322 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 777, 809 |
| FaqI | GGGAC | 6 cut(s) | 459, 918, 1709, 2064, 2084, 2304 |
| FauI | CCCGC | 2 cut(s) | 569, 1810 |
| FauNDI | CATATG | 1 cut(s) | 2508 |
| FbaI | TGATCA | 1 cut(s) | 3268 |
| FriOI | GRGCYC | 2 cut(s) | 206, 562 |
| FspAI | RTGCGCAY | 1 cut(s) | 1338 |
| FspI | TGCGCA | 1 cut(s) | 1338 |
| GlaI | GCGC | 4 cut(s) | 1338, 2138, 2885, 4062 |
| GsuI | CTGGAG | 3 cut(s) | 1138, 2772, 4147 |
| HaeIII | GGCC | 7 cut(s) | 560, 1080, 1419, 1748, 2081, 2357, 2812 |
| HapII | CCGG | 3 cut(s) | 30, 182, 2378 |
| HgaI | GACGC | 3 cut(s) | 920, 1335, 3199 |
| HhaI | GCGC | 4 cut(s) | 1339, 2139, 2886, 4063 |
| Hin1I | GRCGYC | 1 cut(s) | 503 |
| Hin6I | GCGC | 4 cut(s) | 1337, 2137, 2884, 4061 |
| HinP1I | GCGC | 4 cut(s) | 1337, 2137, 2884, 4061 |
| HincII | GTYRAC | 2 cut(s) | 714, 1658 |
| HindII | GTYRAC | 2 cut(s) | 714, 1658 |
| HpaII | CCGG | 3 cut(s) | 30, 182, 2378 |
| Hpy166II | GTNNAC | 8 cut(s) | 507, 548, 714, 1142, 1658, 2630, 3749, 4054 |
| Hpy8I | GTNNAC | 8 cut(s) | 507, 548, 714, 1142, 1658, 2630, 3749, 4054 |
| Hpy99I | CGWCG | 2 cut(s) | 298, 1509 |
| HpyCH4III | ACNGT | 8 cut(s) | 227, 835, 1598, 2276, 2411, 3044, 3217, 4153 |
| HpyCH4IV | ACGT | 7 cut(s) | 296, 503, 1903, 2346, 2722, 2992, 3160 |
| HpySE526I | ACGT | 7 cut(s) | 296, 503, 1903, 2346, 2722, 2992, 3160 |
| Hsp92I | GRCGYC | 1 cut(s) | 503 |
| HspAI | GCGC | 4 cut(s) | 1337, 2137, 2884, 4061 |
| Ksp22I | TGATCA | 1 cut(s) | 3268 |
| LguI | GCTCTTC | 2 cut(s) | 780, 2212 |
| LmnI | GCTCC | 6 cut(s) | 170, 845, 1206, 1450, 2440, 3347 |
| MabI | ACCWGGT | 1 cut(s) | 3457 |
| MaeII | ACGT | 7 cut(s) | 296, 503, 1903, 2346, 2722, 2992, 3160 |
| MaeIII | GTNAC | 7 cut(s) | 1671, 1810, 2872, 3211, 3217, 3614, 3981 |
| MbiI | CCGCTC | 1 cut(s) | 2144 |
| MfeI | CAATTG | 1 cut(s) | 1686 |
| MflI | RGATCY | 4 cut(s) | 514, 893, 2110, 2582 |
| MhlI | GDGCHC | 6 cut(s) | 206, 562, 1588, 1972, 2632, 3819 |
| MlyI | GAGTC | 6 cut(s) | 205, 859, 2046, 2560, 2888, 3656 |
| MmeI | TCCRAC | 6 cut(s) | 354, 1921, 2500, 2624, 2785, 3301 |
| Mph1103I | ATGCAT | 1 cut(s) | 2961 |
| MroXI | GAANNNNTTC | 2 cut(s) | 603, 789 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1209, 1313 |
| MspA1I | CMGCKG | 3 cut(s) | 1114, 1628, 1808 |
| MspI | CCGG | 3 cut(s) | 30, 182, 2378 |
| MunI | CAATTG | 1 cut(s) | 1686 |
| Mva1269I | GAATGC | 1 cut(s) | 2785 |
| MvaI | CCWGG | 9 cut(s) | 499, 1005, 1156, 1175, 1436, 1496, 2814, 3459, 4111 |
| NciI | CCSGG | 1 cut(s) | 2379 |
| NcoI | CCATGG | 1 cut(s) | 2322 |
| NdeI | CATATG | 1 cut(s) | 2508 |
| NmeAIII | GCCGAG | 1 cut(s) | 2107 |
| NmuCI | GTSAC | 5 cut(s) | 1671, 1810, 3211, 3614, 3981 |
| NsbI | TGCGCA | 1 cut(s) | 1338 |
| NsiI | ATGCAT | 1 cut(s) | 2961 |
| NspI | RCATGY | 5 cut(s) | 416, 1109, 1343, 3228, 3747 |
| NspV | TTCGAA | 1 cut(s) | 14 |
| OliI | CACNNNNGTG | 2 cut(s) | 1209, 1313 |
| PaeI | GCATGC | 1 cut(s) | 1343 |
| PagI | TCATGA | 1 cut(s) | 2350 |
| PaqCI | CACCTGC | 1 cut(s) | 2324 |
| PceI | AGGCCT | 2 cut(s) | 2357, 2812 |
| PciI | ACATGT | 2 cut(s) | 412, 1105 |
| PciSI | GCTCTTC | 2 cut(s) | 780, 2212 |
| PctI | GAATGC | 1 cut(s) | 2785 |
| PdmI | GAANNNNTTC | 2 cut(s) | 603, 789 |
| PfoI | TCCNGGA | 1 cut(s) | 1434 |
| PleI | GAGTC | 6 cut(s) | 204, 859, 2046, 2559, 2888, 3656 |
| PmaCI | CACGTG | 1 cut(s) | 3161 |
| PmlI | CACGTG | 1 cut(s) | 3161 |
| PpsI | GAGTC | 6 cut(s) | 204, 859, 2046, 2559, 2888, 3656 |
| Ppu21I | YACGTR | 2 cut(s) | 2723, 3161 |
| PpuMI | RGGWCCY | 3 cut(s) | 446, 3800, 4079 |
| PscI | ACATGT | 2 cut(s) | 412, 1105 |
| PshBI | ATTAAT | 1 cut(s) | 2418 |
| PsiI | TTATAA | 2 cut(s) | 2064, 3507 |
| Psp124BI | GAGCTC | 1 cut(s) | 206 |
| Psp1406I | AACGTT | 1 cut(s) | 1903 |
| Psp5II | RGGWCCY | 3 cut(s) | 446, 3800, 4079 |
| Psp6I | CCWGG | 9 cut(s) | 497, 1003, 1154, 1173, 1434, 1494, 2812, 3457, 4109 |
| PspCI | CACGTG | 1 cut(s) | 3161 |
| PspEI | GGTNACC | 1 cut(s) | 3614 |
| PspGI | CCWGG | 9 cut(s) | 497, 1003, 1154, 1173, 1434, 1494, 2812, 3457, 4109 |
| PspOMI | GGGCCC | 1 cut(s) | 558 |
| PspPPI | RGGWCCY | 3 cut(s) | 446, 3800, 4079 |
| PstI | CTGCAG | 1 cut(s) | 3020 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3719 |
| PsuI | RGATCY | 4 cut(s) | 514, 893, 2110, 2582 |
| PvuII | CAGCTG | 3 cut(s) | 1114, 1628, 1808 |
| RsaI | GTAC | 9 cut(s) | 662, 832, 1143, 1221, 1239, 1699, 2383, 2721, 3463 |
| RsaNI | GTAC | 9 cut(s) | 661, 831, 1142, 1220, 1238, 1698, 2382, 2720, 3462 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1209, 1313 |
| SacI | GAGCTC | 1 cut(s) | 206 |
| SapI | GCTCTTC | 2 cut(s) | 780, 2212 |
| ScaI | AGTACT | 1 cut(s) | 1221 |
| SchI | GAGTC | 6 cut(s) | 205, 859, 2046, 2560, 2888, 3656 |
| SduI | GDGCHC | 6 cut(s) | 206, 562, 1588, 1972, 2632, 3819 |
| SexAI | ACCWGGT | 1 cut(s) | 3457 |
| SfcI | CTRYAG | 2 cut(s) | 3016, 4152 |
| SfuI | TTCGAA | 1 cut(s) | 14 |
| SinI | GGWCC | 6 cut(s) | 446, 959, 2318, 3467, 3800, 4079 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1209, 1313 |
| SmlI | CTYRAG | 5 cut(s) | 436, 1587, 2054, 2569, 2799 |
| SmoI | CTYRAG | 5 cut(s) | 436, 1587, 2054, 2569, 2799 |
| SphI | GCATGC | 1 cut(s) | 1343 |
| SseBI | AGGCCT | 2 cut(s) | 2357, 2812 |
| SsiI | CCGC | 7 cut(s) | 562, 1803, 2022, 2142, 2194, 2197, 3555 |
| SspI | AATATT | 2 cut(s) | 643, 1252 |
| SstI | GAGCTC | 1 cut(s) | 206 |
| StuI | AGGCCT | 2 cut(s) | 2357, 2812 |
| StyI | CCWWGG | 2 cut(s) | 1727, 2322 |
| TaaI | ACNGT | 8 cut(s) | 227, 835, 1598, 2276, 2411, 3044, 3217, 4153 |
| TaiI | ACGT | 7 cut(s) | 299, 506, 1906, 2349, 2725, 2995, 3163 |
| TatI | WGTACW | 4 cut(s) | 660, 830, 1141, 1219 |
| TauI | GCSGC | 1 cut(s) | 2197 |
| TseFI | GTSAC | 5 cut(s) | 1671, 1810, 3211, 3614, 3981 |
| Tsp45I | GTSAC | 5 cut(s) | 1671, 1810, 3211, 3614, 3981 |
| TspGWI | ACGGA | 1 cut(s) | 2671 |
| VneI | GTGCAC | 1 cut(s) | 2628 |
| VpaK11BI | GGWCC | 6 cut(s) | 446, 959, 2318, 3467, 3800, 4079 |
| VspI | ATTAAT | 1 cut(s) | 2418 |
| XapI | RAATTY | 7 cut(s) | 668, 904, 1978, 2090, 2901, 3520, 3753 |
| XbaI | TCTAGA | 1 cut(s) | 517 |
| XceI | RCATGY | 5 cut(s) | 416, 1109, 1343, 3228, 3747 |
| XmnI | GAANNNNTTC | 2 cut(s) | 603, 789 |
| ZraI | GACGTC | 1 cut(s) | 504 |
| ZrmI | AGTACT | 1 cut(s) | 1221 |
| Zsp2I | ATGCAT | 1 cut(s) | 2961 |