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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G64570 |
| fragaria_vesca | FvH4_3g02070 |
| malus_domestica | MD05G1346800.v1.1 MD05G1347100.v1.1 MD05G1347200.v1.1 MD10G1321400.v1.1 |
| prunus_persica | Prupe.4G020900_v2.0.a1 Prupe.4G020900_v2.0.a1 Prupe.4G020900_v2.0.a1 |
| pyrus_communis | pycom05g31490 pycom10g27210 |
| rosa_chinensis | RchiOBHm_Chr5g0002971 RchiOBHm_Chr5g0003031 RchiOBHm_Chr5g0003091 |
| rosa_laevigata | RLG00000031075 |
| rosa_multiflora | Rmu_sc0000146.1_g000022 Rmu_sc0013603.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00072910 |
| rosa_rugosa | Rorug04G0400900 |
| rosa_samantha | Rh5AG024000 Rh5AG025000 Rh5BG026700 Rh5CG026800 Rh5CG027800 Rh5DG025300 |
| rosa_wichuraiana | Rw5G002270 Rw5G002320 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2061, 3504 |
| AarI | CACCTGC | 2 cut(s) | 2321, 2732 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 919 |
| AatII | GACGTC | 2 cut(s) | 243, 503 |
| Acc16I | TGCGCA | 1 cut(s) | 1335 |
| Acc36I | ACCTGC | 3 cut(s) | 2321, 2732, 3808 |
| AccB1I | GGYRCC | 3 cut(s) | 1399, 1966, 2762 |
| AccBSI | CCGCTC | 1 cut(s) | 3452 |
| AccII | CGCG | 2 cut(s) | 295, 3159 |
| AccIII | TCCGGA | 1 cut(s) | 398 |
| AclI | AACGTT | 1 cut(s) | 1900 |
| AclWI | GGATC | 6 cut(s) | 2102, 2574, 2587, 3361, 3590, 3742 |
| AcoI | YGGCCR | 1 cut(s) | 2076 |
| AcsI | RAATTY | 6 cut(s) | 901, 1975, 2087, 2898, 3517, 3750 |
| AcuI | CTGAAG | 7 cut(s) | 324, 381, 486, 2933, 3000, 3687, 3738 |
| AcyI | GRCGYC | 2 cut(s) | 240, 500 |
| AfaI | GTAC | 9 cut(s) | 659, 829, 1140, 1218, 1236, 1696, 2380, 2718, 3460 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 36, 2130, 2984, 3341, 3461, 4098, 4113 |
| AflIII | ACRYGT | 3 cut(s) | 293, 409, 1102 |
| AjnI | CCWGG | 8 cut(s) | 494, 1000, 1151, 1170, 1431, 1491, 3454, 4106 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1887, 1919 |
| AleI | CACNNNNGTG | 2 cut(s) | 1206, 1310 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 380, 412 |
| Alw21I | GWGCWC | 4 cut(s) | 206, 1585, 2629, 3816 |
| Alw26I | GTCTC | 6 cut(s) | 284, 1390, 2365, 2695, 3624, 4006 |
| Alw44I | GTGCAC | 1 cut(s) | 2625 |
| AlwI | GGATC | 6 cut(s) | 2102, 2574, 2587, 3361, 3590, 3742 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 2747, 3716 |
| Aor13HI | TCCGGA | 1 cut(s) | 398 |
| AoxI | GGCC | 7 cut(s) | 555, 1075, 1414, 1743, 2076, 2352, 3118 |
| ApaI | GGGCCC | 1 cut(s) | 559 |
| ApaLI | GTGCAC | 1 cut(s) | 2625 |
| ApoI | RAATTY | 6 cut(s) | 901, 1975, 2087, 2898, 3517, 3750 |
| AseI | ATTAAT | 1 cut(s) | 2415 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 600, 786 |
| AspLEI | GCGC | 4 cut(s) | 1336, 2136, 2883, 4060 |
| AspS9I | GGNCC | 9 cut(s) | 443, 555, 556, 956, 1415, 1744, 2315, 3797, 4076 |
| AsuC2I | CCSGG | 2 cut(s) | 2125, 2376 |
| AsuII | TTCGAA | 1 cut(s) | 14 |
| AsuNHI | GCTAGC | 1 cut(s) | 2641 |
| AvaII | GGWCC | 5 cut(s) | 443, 956, 2315, 3797, 4076 |
| BaeGI | GKGCMC | 3 cut(s) | 559, 1969, 2629 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1473, 1506 |
| BamHI | GGATCC | 1 cut(s) | 2579 |
| BanI | GGYRCC | 3 cut(s) | 1399, 1966, 2762 |
| BanII | GRGCYC | 2 cut(s) | 206, 559 |
| BauI | CACGAG | 1 cut(s) | 985 |
| BbsI | GAAGAC | 2 cut(s) | 1338, 2895 |
| Bbv12I | GWGCWC | 4 cut(s) | 206, 1585, 2629, 3816 |
| BbvCI | CCTCAGC | 2 cut(s) | 1621, 3063 |
| BceAI | ACGGC | 3 cut(s) | 1520, 2063, 2980 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1931, 1965, 3941, 3975 |
| BciT130I | CCWGG | 8 cut(s) | 496, 1002, 1153, 1172, 1433, 1493, 3456, 4108 |
| BciVI | GTATCC | 1 cut(s) | 3907 |
| BclI | TGATCA | 1 cut(s) | 3265 |
| BcnI | CCSGG | 2 cut(s) | 2125, 2376 |
| BcoDI | GTCTC | 6 cut(s) | 284, 1390, 2365, 2695, 3624, 4006 |
| BfmI | CTRYAG | 2 cut(s) | 1677, 3013 |
| BfuAI | ACCTGC | 3 cut(s) | 2321, 2732, 3808 |
| BfuI | GTATCC | 1 cut(s) | 3907 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2245 |
| BglII | AGATCT | 2 cut(s) | 511, 890 |
| BmcAI | AGTACT | 1 cut(s) | 1218 |
| Bme18I | GGWCC | 5 cut(s) | 443, 956, 2315, 3797, 4076 |
| BmgT120I | GGNCC | 9 cut(s) | 443, 555, 556, 956, 1415, 1744, 2315, 3797, 4076 |
| BmrI | ACTGGG | 1 cut(s) | 2578 |
| BmtI | GCTAGC | 1 cut(s) | 2645 |
| BmuI | ACTGGG | 1 cut(s) | 2578 |
| BpiI | GAAGAC | 2 cut(s) | 1338, 2895 |
| BpmI | CTGGAG | 3 cut(s) | 1135, 2769, 4144 |
| Bpu10I | CCTNAGC | 3 cut(s) | 568, 1621, 3063 |
| Bpu14I | TTCGAA | 1 cut(s) | 14 |
| BpuEI | CTTGAG | 4 cut(s) | 418, 1569, 2551, 2781 |
| BpuMI | CCSGG | 2 cut(s) | 2125, 2376 |
| BsaAI | YACGTR | 1 cut(s) | 2720 |
| BsaBI | GATNNNNATC | 4 cut(s) | 153, 453, 3171, 3420 |
| BsaHI | GRCGYC | 2 cut(s) | 240, 500 |
| BsaI | GGTCTC | 1 cut(s) | 2365 |
| BsaJI | CCNNGG | 8 cut(s) | 1001, 1170, 1492, 1724, 2123, 2143, 2319, 4106 |
| BsaWI | WCCGGW | 1 cut(s) | 398 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2362, 2392 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 36, 2130, 2984, 3341, 3461, 4098, 4113 |
| Bse3DI | GCAATG | 1 cut(s) | 923 |
| Bse8I | GATNNNNATC | 4 cut(s) | 153, 453, 3171, 3420 |
| BseAI | TCCGGA | 1 cut(s) | 398 |
| BseBI | CCWGG | 8 cut(s) | 496, 1002, 1153, 1172, 1433, 1493, 3456, 4108 |
| BseDI | CCNNGG | 8 cut(s) | 1001, 1170, 1492, 1724, 2123, 2143, 2319, 4106 |
| BseJI | GATNNNNATC | 4 cut(s) | 153, 453, 3171, 3420 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 36, 2130, 2984, 3341, 3461, 4098, 4113 |
| BseMI | GCAATG | 1 cut(s) | 923 |
| BseRI | GAGGAG | 7 cut(s) | 128, 149, 764, 2424, 2943, 3716, 3950 |
| BseSI | GKGCMC | 3 cut(s) | 559, 1969, 2629 |
| BsgI | GTGCAG | 3 cut(s) | 1127, 2328, 3216 |
| Bsh1236I | CGCG | 2 cut(s) | 295, 3159 |
| BshFI | GGCC | 7 cut(s) | 557, 1077, 1416, 1745, 2078, 2354, 3120 |
| BshNI | GGYRCC | 3 cut(s) | 1399, 1966, 2762 |
| BsiHKAI | GWGCWC | 4 cut(s) | 206, 1585, 2629, 3816 |
| BsiSI | CCGG | 4 cut(s) | 182, 399, 2125, 2375 |
| BslFI | GGGAC | 6 cut(s) | 456, 915, 1706, 2061, 2081, 2301 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 36, 2130, 2984, 3341, 3461, 4098, 4113 |
| BsmAI | GTCTC | 6 cut(s) | 284, 1390, 2365, 2695, 3624, 4006 |
| BsmBI | CGTCTC | 1 cut(s) | 284 |
| BsmFI | GGGAC | 6 cut(s) | 456, 915, 1706, 2061, 2081, 2301 |
| BsmI | GAATGC | 1 cut(s) | 2782 |
| BsnI | GGCC | 7 cut(s) | 557, 1077, 1416, 1745, 2078, 2354, 3120 |
| Bso31I | GGTCTC | 1 cut(s) | 2365 |
| Bsp119I | TTCGAA | 1 cut(s) | 14 |
| Bsp120I | GGGCCC | 1 cut(s) | 555 |
| Bsp1286I | GDGCHC | 6 cut(s) | 206, 559, 1585, 1969, 2629, 3816 |
| Bsp13I | TCCGGA | 1 cut(s) | 398 |
| Bsp19I | CCATGG | 1 cut(s) | 2319 |
| BspANI | GGCC | 7 cut(s) | 557, 1077, 1416, 1745, 2078, 2354, 3120 |
| BspEI | TCCGGA | 1 cut(s) | 398 |
| BspFNI | CGCG | 2 cut(s) | 295, 3159 |
| BspHI | TCATGA | 1 cut(s) | 2347 |
| BspMAI | CTGCAG | 1 cut(s) | 3017 |
| BspMI | ACCTGC | 3 cut(s) | 2321, 2732, 3808 |
| BspOI | GCTAGC | 1 cut(s) | 2645 |
| BspPI | GGATC | 6 cut(s) | 2102, 2574, 2587, 3361, 3590, 3742 |
| BspQI | GCTCTTC | 2 cut(s) | 777, 2209 |
| BspT104I | TTCGAA | 1 cut(s) | 14 |
| BspT107I | GGYRCC | 3 cut(s) | 1399, 1966, 2762 |
| BspTNI | GGTCTC | 1 cut(s) | 2365 |
| BsrBI | CCGCTC | 1 cut(s) | 3452 |
| BsrDI | GCAATG | 1 cut(s) | 923 |
| BssECI | CCNNGG | 8 cut(s) | 1001, 1170, 1492, 1724, 2123, 2143, 2319, 4106 |
| BssNI | GRCGYC | 2 cut(s) | 240, 500 |
| BssSI | CACGAG | 1 cut(s) | 985 |
| BssT1I | CCWWGG | 3 cut(s) | 1724, 2143, 2319 |
| Bst2BI | CACGAG | 1 cut(s) | 985 |
| Bst2UI | CCWGG | 8 cut(s) | 496, 1002, 1153, 1172, 1433, 1493, 3456, 4108 |
| Bst4CI | ACNGT | 7 cut(s) | 832, 1595, 2273, 2408, 3041, 3214, 4150 |
| Bst6I | CTCTTC | 6 cut(s) | 114, 648, 777, 2019, 2209, 3642 |
| BstACI | GRCGYC | 2 cut(s) | 240, 500 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 2534, 2962 |
| BstBAI | YACGTR | 1 cut(s) | 2720 |
| BstBI | TTCGAA | 1 cut(s) | 14 |
| BstC8I | GCNNGC | 4 cut(s) | 297, 559, 1338, 2643 |
| BstDSI | CCRYGG | 1 cut(s) | 2319 |
| BstEII | GGTNACC | 1 cut(s) | 3611 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 3339 |
| BstFNI | CGCG | 2 cut(s) | 295, 3159 |
| BstHHI | GCGC | 4 cut(s) | 1336, 2136, 2883, 4060 |
| BstMAI | GTCTC | 6 cut(s) | 284, 1390, 2365, 2695, 3624, 4006 |
| BstNI | CCWGG | 8 cut(s) | 496, 1002, 1153, 1172, 1433, 1493, 3456, 4108 |
| BstNSI | RCATGY | 5 cut(s) | 413, 1106, 1340, 3225, 3744 |
| BstPI | GGTNACC | 1 cut(s) | 3611 |
| BstSFI | CTRYAG | 2 cut(s) | 1677, 3013 |
| BstSLI | GKGCMC | 3 cut(s) | 559, 1969, 2629 |
| BstUI | CGCG | 2 cut(s) | 295, 3159 |
| BstV2I | GAAGAC | 2 cut(s) | 1338, 2895 |
| BstX2I | RGATCY | 5 cut(s) | 511, 890, 2107, 2579, 3366 |
| BstYI | RGATCY | 5 cut(s) | 511, 890, 2107, 2579, 3366 |
| BsuI | GTATCC | 1 cut(s) | 3907 |
| BsuRI | GGCC | 7 cut(s) | 557, 1077, 1416, 1745, 2078, 2354, 3120 |
| BtgI | CCRYGG | 1 cut(s) | 2319 |
| BtsI | GCAGTG | 4 cut(s) | 672, 1059, 2135, 4171 |
| BveI | ACCTGC | 3 cut(s) | 2321, 2732, 3808 |
| Cac8I | GCNNGC | 4 cut(s) | 297, 559, 1338, 2643 |
| CaiI | CAGNNNCTG | 2 cut(s) | 2747, 3716 |
| CciI | TCATGA | 1 cut(s) | 2347 |
| CfoI | GCGC | 4 cut(s) | 1336, 2136, 2883, 4060 |
| Cfr13I | GGNCC | 9 cut(s) | 443, 555, 556, 956, 1415, 1744, 2315, 3797, 4076 |
| CseI | GACGC | 4 cut(s) | 301, 917, 1332, 3196 |
| Csp6I | GTAC | 9 cut(s) | 658, 828, 1139, 1217, 1235, 1695, 2379, 2717, 3459 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1434, 1469 |
| CviQI | GTAC | 9 cut(s) | 658, 828, 1139, 1217, 1235, 1695, 2379, 2717, 3459 |
| DraI | TTTAAA | 2 cut(s) | 2208, 2707 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 919 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 919 |
| EaeI | YGGCCR | 1 cut(s) | 2076 |
| Eam1104I | CTCTTC | 6 cut(s) | 114, 648, 777, 2019, 2209, 3642 |
| EarI | CTCTTC | 6 cut(s) | 114, 648, 777, 2019, 2209, 3642 |
| EciI | GGCGGA | 2 cut(s) | 261, 955 |
| Ecl136II | GAGCTC | 1 cut(s) | 204 |
| Eco130I | CCWWGG | 3 cut(s) | 1724, 2143, 2319 |
| Eco147I | AGGCCT | 1 cut(s) | 2354 |
| Eco24I | GRGCYC | 2 cut(s) | 206, 559 |
| Eco31I | GGTCTC | 1 cut(s) | 2365 |
| Eco32I | GATATC | 1 cut(s) | 2031 |
| Eco47I | GGWCC | 5 cut(s) | 443, 956, 2315, 3797, 4076 |
| Eco53kI | GAGCTC | 1 cut(s) | 204 |
| Eco57I | CTGAAG | 7 cut(s) | 324, 381, 486, 2933, 3000, 3687, 3738 |
| Eco91I | GGTNACC | 1 cut(s) | 3611 |
| EcoICRI | GAGCTC | 1 cut(s) | 204 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 3339 |
| EcoO109I | RGGNCCY | 3 cut(s) | 443, 3797, 4076 |
| EcoO65I | GGTNACC | 1 cut(s) | 3611 |
| EcoRII | CCWGG | 8 cut(s) | 494, 1000, 1151, 1170, 1431, 1491, 3454, 4106 |
| EcoRV | GATATC | 1 cut(s) | 2031 |
| EcoT14I | CCWWGG | 3 cut(s) | 1724, 2143, 2319 |
| EcoT22I | ATGCAT | 2 cut(s) | 2530, 2958 |
| EcoT38I | GRGCYC | 2 cut(s) | 206, 559 |
| ErhI | CCWWGG | 3 cut(s) | 1724, 2143, 2319 |
| Esp3I | CGTCTC | 1 cut(s) | 284 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 774, 806 |
| FaqI | GGGAC | 6 cut(s) | 456, 915, 1706, 2061, 2081, 2301 |
| FauI | CCCGC | 2 cut(s) | 566, 1807 |
| FauNDI | CATATG | 1 cut(s) | 2505 |
| FbaI | TGATCA | 1 cut(s) | 3265 |
| FriOI | GRGCYC | 2 cut(s) | 206, 559 |
| FspAI | RTGCGCAY | 1 cut(s) | 1335 |
| FspI | TGCGCA | 1 cut(s) | 1335 |
| GlaI | GCGC | 4 cut(s) | 1335, 2135, 2882, 4059 |
| GsuI | CTGGAG | 3 cut(s) | 1135, 2769, 4144 |
| HaeIII | GGCC | 7 cut(s) | 557, 1077, 1416, 1745, 2078, 2354, 3120 |
| HapII | CCGG | 4 cut(s) | 182, 399, 2125, 2375 |
| HgaI | GACGC | 4 cut(s) | 301, 917, 1332, 3196 |
| HhaI | GCGC | 4 cut(s) | 1336, 2136, 2883, 4060 |
| Hin1I | GRCGYC | 2 cut(s) | 240, 500 |
| Hin6I | GCGC | 4 cut(s) | 1334, 2134, 2881, 4058 |
| HinP1I | GCGC | 4 cut(s) | 1334, 2134, 2881, 4058 |
| HincII | GTYRAC | 3 cut(s) | 262, 711, 1655 |
| HindII | GTYRAC | 3 cut(s) | 262, 711, 1655 |
| HindIII | AAGCTT | 1 cut(s) | 2055 |
| HpaII | CCGG | 4 cut(s) | 182, 399, 2125, 2375 |
| Hpy166II | GTNNAC | 9 cut(s) | 262, 504, 545, 711, 1139, 1655, 2627, 3746, 4051 |
| Hpy8I | GTNNAC | 9 cut(s) | 262, 504, 545, 711, 1139, 1655, 2627, 3746, 4051 |
| Hpy99I | CGWCG | 2 cut(s) | 245, 1506 |
| HpyCH4III | ACNGT | 7 cut(s) | 832, 1595, 2273, 2408, 3041, 3214, 4150 |
| HpyCH4IV | ACGT | 6 cut(s) | 240, 500, 1900, 2343, 2719, 2989 |
| HpySE526I | ACGT | 6 cut(s) | 240, 500, 1900, 2343, 2719, 2989 |
| Hsp92I | GRCGYC | 2 cut(s) | 240, 500 |
| HspAI | GCGC | 4 cut(s) | 1334, 2134, 2881, 4058 |
| Kpn2I | TCCGGA | 1 cut(s) | 398 |
| Ksp22I | TGATCA | 1 cut(s) | 3265 |
| LguI | GCTCTTC | 2 cut(s) | 777, 2209 |
| LmnI | GCTCC | 8 cut(s) | 170, 209, 842, 1203, 1447, 2437, 3344, 3457 |
| MaeII | ACGT | 6 cut(s) | 240, 500, 1900, 2343, 2719, 2989 |
| MaeIII | GTNAC | 8 cut(s) | 1668, 1807, 2869, 3208, 3214, 3611, 3978, 4166 |
| MbiI | CCGCTC | 1 cut(s) | 3452 |
| MfeI | CAATTG | 1 cut(s) | 1683 |
| MflI | RGATCY | 5 cut(s) | 511, 890, 2107, 2579, 3366 |
| MhlI | GDGCHC | 6 cut(s) | 206, 559, 1585, 1969, 2629, 3816 |
| MluI | ACGCGT | 1 cut(s) | 293 |
| MlyI | GAGTC | 6 cut(s) | 257, 856, 2043, 2557, 2885, 3653 |
| MmeI | TCCRAC | 6 cut(s) | 351, 1918, 2497, 2621, 2782, 3298 |
| Mph1103I | ATGCAT | 2 cut(s) | 2530, 2958 |
| MroI | TCCGGA | 1 cut(s) | 398 |
| MroXI | GAANNNNTTC | 2 cut(s) | 600, 786 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1206, 1310 |
| MspA1I | CMGCKG | 3 cut(s) | 1111, 1625, 1805 |
| MspI | CCGG | 4 cut(s) | 182, 399, 2125, 2375 |
| MunI | CAATTG | 1 cut(s) | 1683 |
| Mva1269I | GAATGC | 1 cut(s) | 2782 |
| MvaI | CCWGG | 8 cut(s) | 496, 1002, 1153, 1172, 1433, 1493, 3456, 4108 |
| MvnI | CGCG | 2 cut(s) | 295, 3159 |
| NciI | CCSGG | 2 cut(s) | 2125, 2376 |
| NcoI | CCATGG | 1 cut(s) | 2319 |
| NdeI | CATATG | 1 cut(s) | 2505 |
| NheI | GCTAGC | 1 cut(s) | 2641 |
| NmuCI | GTSAC | 6 cut(s) | 1668, 1807, 3208, 3611, 3978, 4166 |
| NsbI | TGCGCA | 1 cut(s) | 1335 |
| NsiI | ATGCAT | 2 cut(s) | 2530, 2958 |
| NspI | RCATGY | 5 cut(s) | 413, 1106, 1340, 3225, 3744 |
| NspV | TTCGAA | 1 cut(s) | 14 |
| OliI | CACNNNNGTG | 2 cut(s) | 1206, 1310 |
| PaeI | GCATGC | 1 cut(s) | 1340 |
| PagI | TCATGA | 1 cut(s) | 2347 |
| PaqCI | CACCTGC | 2 cut(s) | 2321, 2732 |
| PceI | AGGCCT | 1 cut(s) | 2354 |
| PciI | ACATGT | 2 cut(s) | 409, 1102 |
| PciSI | GCTCTTC | 2 cut(s) | 777, 2209 |
| PctI | GAATGC | 1 cut(s) | 2782 |
| PdmI | GAANNNNTTC | 2 cut(s) | 600, 786 |
| PfoI | TCCNGGA | 1 cut(s) | 1431 |
| PleI | GAGTC | 6 cut(s) | 257, 856, 2043, 2556, 2885, 3653 |
| PpsI | GAGTC | 6 cut(s) | 257, 856, 2043, 2556, 2885, 3653 |
| Ppu21I | YACGTR | 1 cut(s) | 2720 |
| PpuMI | RGGWCCY | 3 cut(s) | 443, 3797, 4076 |
| PscI | ACATGT | 2 cut(s) | 409, 1102 |
| PshBI | ATTAAT | 1 cut(s) | 2415 |
| PsiI | TTATAA | 2 cut(s) | 2061, 3504 |
| Psp124BI | GAGCTC | 1 cut(s) | 206 |
| Psp1406I | AACGTT | 1 cut(s) | 1900 |
| Psp5II | RGGWCCY | 3 cut(s) | 443, 3797, 4076 |
| Psp6I | CCWGG | 8 cut(s) | 494, 1000, 1151, 1170, 1431, 1491, 3454, 4106 |
| PspEI | GGTNACC | 1 cut(s) | 3611 |
| PspGI | CCWGG | 8 cut(s) | 494, 1000, 1151, 1170, 1431, 1491, 3454, 4106 |
| PspOMI | GGGCCC | 1 cut(s) | 555 |
| PspPI | GGNCC | 9 cut(s) | 443, 555, 556, 956, 1415, 1744, 2315, 3797, 4076 |
| PspPPI | RGGWCCY | 3 cut(s) | 443, 3797, 4076 |
| PstI | CTGCAG | 1 cut(s) | 3017 |
| PstNI | CAGNNNCTG | 2 cut(s) | 2747, 3716 |
| PsuI | RGATCY | 5 cut(s) | 511, 890, 2107, 2579, 3366 |
| PvuII | CAGCTG | 3 cut(s) | 1111, 1625, 1805 |
| RsaI | GTAC | 9 cut(s) | 659, 829, 1140, 1218, 1236, 1696, 2380, 2718, 3460 |
| RsaNI | GTAC | 9 cut(s) | 658, 828, 1139, 1217, 1235, 1695, 2379, 2717, 3459 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1206, 1310 |
| SacI | GAGCTC | 1 cut(s) | 206 |
| SapI | GCTCTTC | 2 cut(s) | 777, 2209 |
| Sau96I | GGNCC | 9 cut(s) | 443, 555, 556, 956, 1415, 1744, 2315, 3797, 4076 |
| ScaI | AGTACT | 1 cut(s) | 1218 |
| SchI | GAGTC | 6 cut(s) | 257, 856, 2043, 2557, 2885, 3653 |
| SduI | GDGCHC | 6 cut(s) | 206, 559, 1585, 1969, 2629, 3816 |
| SfcI | CTRYAG | 2 cut(s) | 1677, 3013 |
| SfuI | TTCGAA | 1 cut(s) | 14 |
| SinI | GGWCC | 5 cut(s) | 443, 956, 2315, 3797, 4076 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1206, 1310 |
| SmlI | CTYRAG | 4 cut(s) | 433, 1584, 2566, 2796 |
| SmoI | CTYRAG | 4 cut(s) | 433, 1584, 2566, 2796 |
| SphI | GCATGC | 1 cut(s) | 1340 |
| SseBI | AGGCCT | 1 cut(s) | 2354 |
| SspI | AATATT | 2 cut(s) | 640, 1249 |
| SstI | GAGCTC | 1 cut(s) | 206 |
| StuI | AGGCCT | 1 cut(s) | 2354 |
| StyI | CCWWGG | 3 cut(s) | 1724, 2143, 2319 |
| TaaI | ACNGT | 7 cut(s) | 832, 1595, 2273, 2408, 3041, 3214, 4150 |
| TaiI | ACGT | 6 cut(s) | 243, 503, 1903, 2346, 2722, 2992 |
| TaqI | TCGA | 8 cut(s) | 14, 594, 865, 1501, 2477, 2546, 2561, 3687 |
| TatI | WGTACW | 4 cut(s) | 657, 827, 1138, 1216 |
| TauI | GCSGC | 2 cut(s) | 2194, 3162 |
| TseFI | GTSAC | 6 cut(s) | 1668, 1807, 3208, 3611, 3978, 4166 |
| Tsp45I | GTSAC | 6 cut(s) | 1668, 1807, 3208, 3611, 3978, 4166 |
| VneI | GTGCAC | 1 cut(s) | 2625 |
| VpaK11BI | GGWCC | 5 cut(s) | 443, 956, 2315, 3797, 4076 |
| VspI | ATTAAT | 1 cut(s) | 2415 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 3339 |
| XapI | RAATTY | 6 cut(s) | 901, 1975, 2087, 2898, 3517, 3750 |
| XbaI | TCTAGA | 1 cut(s) | 514 |
| XceI | RCATGY | 5 cut(s) | 413, 1106, 1340, 3225, 3744 |
| XmnI | GAANNNNTTC | 2 cut(s) | 600, 786 |
| ZraI | GACGTC | 2 cut(s) | 241, 501 |
| ZrmI | AGTACT | 1 cut(s) | 1218 |
| Zsp2I | ATGCAT | 2 cut(s) | 2530, 2958 |