Rh5DG025400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010604
Physical Location & Seq
Forward (+)
1771185 .. 1772285
1101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG025400.1

Sequence Viewer

Length: 546 bp
ATGAACAAGGGCAAGTATTACATCCCATGGTCTCGCCTTGTTTGGGTGAGAGAGGGGATTATGTGCCCGCTCACAGTCACTCTACTGCTCGGGCAGAGGAGACCCGCCGTCGAGCGCATGGTCACAGCACGGTGGATGTACGACCAACACGTCACTGAGGAAATTTTGAGAGACTATTTTGACAGGTCGGGCTTTAACTTGCAAGAGGTGGAGATCCTAGAGCCTGAGCTGGGATATCTGTTATTCGAGAGTAATTATGCAGTCCATAATCTGATGTATGGACAGGAGGAAGCAGAGTATTATGTGGGTGACTCAAATGGCACTATTCTGTTGGCCAAGCCCGAAAATTTGTATCTGACATTTGATTCAGCCAACGACTATCTGAAGTGTTCTTGCGCACAAACAATGATGGACTGGCCATCGTCTAATATAAGCTCTGCATGTGGCTGGCCGGATAAGATGGAAAGGACAGAGTATGGACTCTTGAAAATAATCCTGGCTTCCCCTTCTCCACATCGCTCAACCCTTTCGATTGGGTGTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.87

Weight (kDa)

4.98

Isoelectric Point (pI)

70.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
Acc16I TGCGCA 1 cut(s) 397
AccBSI CCGCTC 1 cut(s) 70
AciI CCGC 2 cut(s) 68, 105
AclWI GGATC 1 cut(s) 208
AcoI YGGCCR 3 cut(s) 333, 416, 449
AcsI RAATTY 2 cut(s) 162, 346
AcuI CTGAAG 1 cut(s) 404
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 2 cut(s) 43, 230
AflIII ACRYGT 1 cut(s) 148
AgsI TTSAA 1 cut(s) 487
AhdI GACNNNNNGTC 1 cut(s) 107
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 495
AluBI AGCT 2 cut(s) 229, 435
AluI AGCT 2 cut(s) 229, 435
Alw26I GTCTC 3 cut(s) 36, 94, 165
AlwI GGATC 1 cut(s) 208
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 3 cut(s) 333, 416, 449
ApoI RAATTY 2 cut(s) 162, 346
AspLEI GCGC 2 cut(s) 117, 398
AsuHPI GGTGA 2 cut(s) 58, 320
AvaI CYCGRG 1 cut(s) 89
BaeGI GKGCMC 1 cut(s) 68
BalI TGGCCA 2 cut(s) 335, 418
BccI CCATC 3 cut(s) 403, 427, 454
BceAI ACGGC 1 cut(s) 92
BciT130I CCWGG 1 cut(s) 497
BcoDI GTCTC 3 cut(s) 36, 94, 165
BfaI CTAG 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 497
BmeRI GACNNNNNGTC 1 cut(s) 107
BmeT110I CYCGRG 1 cut(s) 89
BmgBI CACGTC 1 cut(s) 151
BmrFI CCNGG 1 cut(s) 497
Bpu10I CCTNAGC 1 cut(s) 225
BsaI GGTCTC 2 cut(s) 36, 94
BsaJI CCNNGG 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 230
Bse1I ACTGG 1 cut(s) 419
BseBI CCWGG 1 cut(s) 497
BseDI CCNNGG 1 cut(s) 26
BseGI GGATG 2 cut(s) 21, 141
BseLI CCNNNNNNNGG 2 cut(s) 43, 230
BseMII CTCAG 2 cut(s) 147, 216
BseNI ACTGG 1 cut(s) 419
BseRI GAGGAG 1 cut(s) 112
BseSI GKGCMC 1 cut(s) 68
BseYI CCCAGC 1 cut(s) 229
BshFI GGCC 3 cut(s) 335, 418, 451
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 1 cut(s) 452
BslI CCNNNNNNNGG 2 cut(s) 43, 230
BsmAI GTCTC 3 cut(s) 36, 94, 165
BsnI GGCC 3 cut(s) 335, 418, 451
Bso31I GGTCTC 2 cut(s) 36, 94
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 68
Bsp143I GATC 1 cut(s) 213
Bsp19I CCATGG 1 cut(s) 26
BspACI CCGC 2 cut(s) 68, 105
BspANI GGCC 3 cut(s) 335, 418, 451
BspCNI CTCAG 2 cut(s) 148, 217
BspPI GGATC 1 cut(s) 208
BspTNI GGTCTC 2 cut(s) 36, 94
BsrBI CCGCTC 1 cut(s) 70
BsrI ACTGG 1 cut(s) 419
BssECI CCNNGG 1 cut(s) 26
BssMI GATC 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 497
Bst4CI ACNGT 2 cut(s) 76, 132
BstC8I GCNNGC 2 cut(s) 68, 449
BstDEI CTNAG 2 cut(s) 156, 225
BstDSI CCRYGG 1 cut(s) 26
BstF5I GGATG 2 cut(s) 21, 141
BstHHI GCGC 2 cut(s) 117, 398
BstKTI GATC 1 cut(s) 216
BstMAI GTCTC 3 cut(s) 36, 94, 165
BstMBI GATC 1 cut(s) 213
BstNI CCWGG 1 cut(s) 497
BstNSI RCATGY 1 cut(s) 444
BstSCI CCNGG 1 cut(s) 495
BstSLI GKGCMC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
BsuRI GGCC 3 cut(s) 335, 418, 451
BtgI CCRYGG 1 cut(s) 26
BtgZI GCGATG 1 cut(s) 500
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 2 cut(s) 21, 141
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 2 cut(s) 68, 449
CfoI GCGC 2 cut(s) 117, 398
Csp6I GTAC 1 cut(s) 139
CviAII CATG 3 cut(s) 27, 118, 441
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 2 cut(s) 156, 225
DpnI GATC 1 cut(s) 215
DpnII GATC 1 cut(s) 213
DrdI GACNNNNNNGTC 1 cut(s) 149
DriI GACNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 3 cut(s) 333, 416, 449
Eam1105I GACNNNNNGTC 1 cut(s) 107
Eco130I CCWWGG 1 cut(s) 26
Eco31I GGTCTC 2 cut(s) 36, 94
Eco32I GATATC 1 cut(s) 236
Eco57I CTGAAG 1 cut(s) 404
Eco88I CYCGRG 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 495
EcoRV GATATC 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 26
FaeI CATG 3 cut(s) 30, 121, 444
FatI CATG 3 cut(s) 26, 117, 440
FauI CCCGC 2 cut(s) 75, 112
FokI GGATG 2 cut(s) 8, 148
FspBI CTAG 1 cut(s) 218
FspI TGCGCA 1 cut(s) 397
GlaI GCGC 2 cut(s) 116, 397
GsaI CCCAGC 1 cut(s) 233
HaeIII GGCC 3 cut(s) 335, 418, 451
HapII CCGG 1 cut(s) 452
HhaI GCGC 2 cut(s) 117, 398
Hin1II CATG 3 cut(s) 30, 121, 444
Hin6I GCGC 2 cut(s) 115, 396
HinP1I GCGC 2 cut(s) 115, 396
HinfI GANTC 3 cut(s) 311, 365, 480
HpaII CCGG 1 cut(s) 452
HphI GGTGA 2 cut(s) 58, 320
Hpy188I TCNGA 3 cut(s) 273, 357, 384
Hpy188III TCNNGA 2 cut(s) 247, 484
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 1 cut(s) 516
HpyCH4III ACNGT 2 cut(s) 76, 132
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 3 cut(s) 202, 260, 440
HpyF3I CTNAG 2 cut(s) 156, 225
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 3 cut(s) 30, 121, 444
HspAI GCGC 2 cut(s) 115, 396
Kzo9I GATC 1 cut(s) 213
LpnPI CCDG 9 cut(s) 169, 215, 237, 269, 400, 433, 465, 482, 509
MaeI CTAG 1 cut(s) 218
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 4 cut(s) 76, 121, 151, 308
MalI GATC 1 cut(s) 215
MbiI CCGCTC 1 cut(s) 70
MboI GATC 1 cut(s) 213
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 1 cut(s) 68
MlsI TGGCCA 2 cut(s) 335, 418
MluCI AATT 3 cut(s) 162, 253, 346
MluNI TGGCCA 2 cut(s) 335, 418
MlyI GAGTC 2 cut(s) 305, 474
MnlI CCTC 5 cut(s) 46, 90, 151, 199, 280
Mox20I TGGCCA 2 cut(s) 335, 418
MscI TGGCCA 2 cut(s) 335, 418
MseI TTAA 1 cut(s) 195
Msp20I TGGCCA 2 cut(s) 335, 418
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 1 cut(s) 497
MvaI CCWGG 1 cut(s) 497
NcoI CCATGG 1 cut(s) 26
NdeII GATC 1 cut(s) 213
NlaIII CATG 3 cut(s) 30, 121, 444
NmuCI GTSAC 4 cut(s) 76, 121, 151, 308
NsbI TGCGCA 1 cut(s) 397
NspI RCATGY 1 cut(s) 444
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfeI GAWTC 1 cut(s) 365
PleI GAGTC 2 cut(s) 305, 474
PpsI GAGTC 2 cut(s) 305, 474
Psp6I CCWGG 1 cut(s) 495
PspFI CCCAGC 1 cut(s) 229
PspGI CCWGG 1 cut(s) 495
PsuI RGATCY 1 cut(s) 213
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
SaqAI TTAA 1 cut(s) 195
Sau3AI GATC 1 cut(s) 213
SchI GAGTC 2 cut(s) 305, 474
ScrFI CCNGG 1 cut(s) 497
SduI GDGCHC 1 cut(s) 68
SetI ASST 5 cut(s) 153, 188, 210, 231, 437
Sse9I AATT 3 cut(s) 162, 253, 346
SsiI CCGC 2 cut(s) 68, 105
SspMI CTAG 1 cut(s) 218
StyD4I CCNGG 1 cut(s) 495
StyI CCWWGG 1 cut(s) 26
TaaI ACNGT 2 cut(s) 76, 132
TaiI ACGT 1 cut(s) 153
TaqI TCGA 3 cut(s) 111, 246, 530
TasI AATT 3 cut(s) 162, 253, 346
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 4 cut(s) 76, 121, 151, 308
Tsp45I GTSAC 4 cut(s) 76, 121, 151, 308
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 160
XapI RAATTY 2 cut(s) 162, 346
XceI RCATGY 1 cut(s) 444
XspI CTAG 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.