Rh5DG159300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008177
Physical Location & Seq
Reverse (-)
16273754 .. 16274308
555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG159300.1

Sequence Viewer

Length: 555 bp
ATGAGGAGAGCGCTGCTAGGATTTCTTGGCGGCGTTCTAGCATTTTTGGGTGGAGGGGTTGGACATTCCAATCTCTCTGTTCACACTATGGGTGATGGCAACAGTGGAAGAGTTCTTCAAAGTCAAGAGGAAGGATTGATAGGATTTTGCTTCCTCTTTATCTCTCTAGTTGCTATTGATAGCAAAGGAAGGTTGTGTTCTTCTTGTGGTGGCGGCCCCGATCGTGTTGGAGGTCAGGCGTTAGCCTTTGGTGGTACGTGCGTTTTGGCTGTCGGTGGAGGATGGCTTCCAGATCGAGCAGCGATCAAGTTTGGTGATCAGGATTCGATCCTTGGATCCTCGGTGGCAGTGGTTTTATCAGCTACCAATGTTGATGGGTTTGAAAACCAGATTAAGATGGTGTCAGGTAGTGCTGATGTCGGTGGAGCTGCTAACATAGTTTGGTGGGCTGTGGCTATCGGACGACAGGTCTCTAATGTCAGTGATGTAGATCTGGGCCGGCTTGTTGGAAGGCATGGTGATGGTCTTGTCGTTGGTGGTGGCGCCCATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

18.54

Weight (kDa)

6.02

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 542
AciI CCGC 2 cut(s) 30, 213
AclWI GGATC 3 cut(s) 322, 330, 343
AcyI GRCGYC 1 cut(s) 543
AfaI GTAC 1 cut(s) 256
AfeI AGCGCT 1 cut(s) 12
AgsI TTSAA 2 cut(s) 119, 383
AhdI GACNNNNNGTC 1 cut(s) 467
AluBI AGCT 2 cut(s) 362, 428
AluI AGCT 2 cut(s) 362, 428
Alw26I GTCTC 1 cut(s) 475
AlwI GGATC 3 cut(s) 322, 330, 343
Aor51HI AGCGCT 1 cut(s) 12
AoxI GGCC 2 cut(s) 214, 496
ApeKI GCWGC 3 cut(s) 13, 299, 428
AspLEI GCGC 2 cut(s) 13, 545
AspS9I GGNCC 2 cut(s) 215, 496
AsuHPI GGTGA 3 cut(s) 104, 326, 530
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BamHI GGATCC 1 cut(s) 335
BanI GGYRCC 1 cut(s) 542
BbvI GCAGC 2 cut(s) 311, 415
BccI CCATC 5 cut(s) 89, 276, 368, 391, 515
BclI TGATCA 1 cut(s) 316
BcoDI GTCTC 1 cut(s) 475
BfaI CTAG 3 cut(s) 17, 38, 167
BfoI RGCGCY 2 cut(s) 14, 546
BglII AGATCT 1 cut(s) 490
BisI GCNGC 5 cut(s) 14, 31, 214, 300, 429
BlsI GCNGC 5 cut(s) 15, 32, 215, 301, 430
BmeRI GACNNNNNGTC 1 cut(s) 467
BmgT120I GGNCC 2 cut(s) 215, 496
BmiI GGNNCC 3 cut(s) 217, 337, 544
BsaAI YACGTR 1 cut(s) 258
BsaBI GATNNNNATC 1 cut(s) 489
BsaHI GRCGYC 1 cut(s) 543
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 2 cut(s) 331, 339
Bse118I RCCGGY 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 489
BseDI CCNNGG 2 cut(s) 331, 339
BseGI GGATG 1 cut(s) 287
BseJI GATNNNNATC 1 cut(s) 489
BseRI GAGGAG 1 cut(s) 19
BseXI GCAGC 2 cut(s) 311, 415
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 2 cut(s) 216, 498
BshNI GGYRCC 1 cut(s) 542
BsiEI CGRYCG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 499
BsmAI GTCTC 1 cut(s) 475
BsnI GGCC 2 cut(s) 216, 498
Bso31I GGTCTC 1 cut(s) 475
Bsp143I GATC 7 cut(s) 220, 292, 303, 316, 327, 335, 490
BspACI CCGC 2 cut(s) 30, 213
BspANI GGCC 2 cut(s) 216, 498
BspLI GGNNCC 3 cut(s) 217, 337, 544
BspPI GGATC 3 cut(s) 322, 330, 343
BspT107I GGYRCC 1 cut(s) 542
BspTNI GGTCTC 1 cut(s) 475
BsrFI RCCGGY 1 cut(s) 498
BssAI RCCGGY 1 cut(s) 498
BssECI CCNNGG 2 cut(s) 331, 339
BssMI GATC 7 cut(s) 220, 292, 303, 316, 327, 335, 490
BssNI GRCGYC 1 cut(s) 543
BssT1I CCWWGG 1 cut(s) 331
Bst4CI ACNGT 1 cut(s) 104
Bst6I CTCTTC 1 cut(s) 103
BstACI GRCGYC 1 cut(s) 543
BstBAI YACGTR 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 500
BstF5I GGATG 1 cut(s) 287
BstH2I RGCGCY 2 cut(s) 14, 546
BstHHI GCGC 2 cut(s) 13, 545
BstKTI GATC 7 cut(s) 223, 295, 306, 319, 330, 338, 493
BstMAI GTCTC 1 cut(s) 475
BstMBI GATC 7 cut(s) 220, 292, 303, 316, 327, 335, 490
BstMCI CGRYCG 1 cut(s) 223
BstV1I GCAGC 2 cut(s) 311, 415
BstX2I RGATCY 2 cut(s) 335, 490
BstYI RGATCY 2 cut(s) 335, 490
BsuRI GGCC 2 cut(s) 216, 498
BtsCI GGATG 1 cut(s) 287
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 3 cut(s) 109, 354, 487
Cac8I GCNNGC 1 cut(s) 500
CfoI GCGC 2 cut(s) 13, 545
Cfr10I RCCGGY 1 cut(s) 498
Cfr13I GGNCC 2 cut(s) 215, 496
Csp6I GTAC 1 cut(s) 255
CviAII CATG 1 cut(s) 515
CviQI GTAC 1 cut(s) 255
DinI GGCGCC 1 cut(s) 544
DpnI GATC 7 cut(s) 222, 294, 305, 318, 329, 337, 492
DpnII GATC 7 cut(s) 220, 292, 303, 316, 327, 335, 490
DriI GACNNNNNGTC 1 cut(s) 467
Eam1104I CTCTTC 1 cut(s) 103
Eam1105I GACNNNNNGTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 331
Eco31I GGTCTC 1 cut(s) 475
Eco47III AGCGCT 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 331
EgeI GGCGCC 1 cut(s) 544
EheI GGCGCC 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 331
FaeI CATG 1 cut(s) 518
FaiI YATR 3 cut(s) 89, 437, 516
FatI CATG 1 cut(s) 514
FbaI TGATCA 1 cut(s) 316
Fnu4HI GCNGC 5 cut(s) 14, 31, 214, 300, 429
FokI GGATG 1 cut(s) 294
Fsp4HI GCNGC 5 cut(s) 14, 31, 214, 300, 429
FspBI CTAG 3 cut(s) 17, 38, 167
GlaI GCGC 2 cut(s) 12, 544
GluI GCNGC 5 cut(s) 14, 31, 214, 300, 429
HaeII RGCGCY 2 cut(s) 14, 546
HaeIII GGCC 2 cut(s) 216, 498
HapII CCGG 1 cut(s) 499
HhaI GCGC 2 cut(s) 13, 545
Hin1I GRCGYC 1 cut(s) 543
Hin1II CATG 1 cut(s) 518
Hin6I GCGC 2 cut(s) 11, 543
HinP1I GCGC 2 cut(s) 11, 543
HinfI GANTC 1 cut(s) 323
HpaII CCGG 1 cut(s) 499
HphI GGTGA 3 cut(s) 104, 326, 530
Hpy166II GTNNAC 1 cut(s) 82
Hpy188I TCNGA 1 cut(s) 461
Hpy188III TCNNGA 3 cut(s) 125, 290, 320
Hpy8I GTNNAC 1 cut(s) 82
HpyAV CCTTC 3 cut(s) 125, 183, 504
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 1 cut(s) 257
HpySE526I ACGT 1 cut(s) 257
Hsp92I GRCGYC 1 cut(s) 543
Hsp92II CATG 1 cut(s) 518
HspAI GCGC 2 cut(s) 11, 543
KasI GGCGCC 1 cut(s) 542
KroI GCCGGC 1 cut(s) 498
KroNI GCCGGC 1 cut(s) 500
Ksp22I TGATCA 1 cut(s) 316
Kzo9I GATC 7 cut(s) 220, 292, 303, 316, 327, 335, 490
LmnI GCTCC 1 cut(s) 425
LpnPI CCDG 8 cut(s) 221, 303, 305, 390, 401, 452, 479, 512
Lsp1109I GCAGC 2 cut(s) 311, 415
MaeI CTAG 3 cut(s) 17, 38, 167
MaeII ACGT 1 cut(s) 257
MalI GATC 7 cut(s) 222, 294, 305, 318, 329, 337, 492
MboI GATC 7 cut(s) 220, 292, 303, 316, 327, 335, 490
MboII GAAGA 3 cut(s) 107, 120, 192
MflI RGATCY 2 cut(s) 335, 490
Mly113I GGCGCC 1 cut(s) 543
MmeI TCCRAC 3 cut(s) 40, 208, 487
MnlI CCTC 6 cut(s) 47, 121, 164, 224, 272, 349
MroNI GCCGGC 1 cut(s) 498
MseI TTAA 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 519
MspI CCGG 1 cut(s) 499
NaeI GCCGGC 1 cut(s) 500
NarI GGCGCC 1 cut(s) 543
NdeII GATC 7 cut(s) 220, 292, 303, 316, 327, 335, 490
NgoMIV GCCGGC 1 cut(s) 498
NlaIII CATG 1 cut(s) 518
NlaIV GGNNCC 3 cut(s) 217, 337, 544
PdiI GCCGGC 1 cut(s) 500
PfeI GAWTC 1 cut(s) 323
PkrI GCNGC 5 cut(s) 15, 32, 215, 301, 430
Ple19I CGATCG 1 cut(s) 223
PluTI GGCGCC 1 cut(s) 546
Ppu21I YACGTR 1 cut(s) 258
PspN4I GGNNCC 3 cut(s) 217, 337, 544
PspPI GGNCC 2 cut(s) 215, 496
PsuI RGATCY 2 cut(s) 335, 490
PvuI CGATCG 1 cut(s) 223
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
RseI CAYNNNNRTG 1 cut(s) 519
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 5 cut(s) 14, 31, 214, 300, 429
Sau3AI GATC 7 cut(s) 220, 292, 303, 316, 327, 335, 490
Sau96I GGNCC 2 cut(s) 215, 496
SetI ASST 7 cut(s) 194, 235, 260, 364, 409, 430, 471
SfoI GGCGCC 1 cut(s) 544
SmiMI CAYNNNNRTG 1 cut(s) 519
SsiI CCGC 2 cut(s) 30, 213
SspDI GGCGCC 1 cut(s) 542
SspMI CTAG 3 cut(s) 17, 38, 167
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 1 cut(s) 260
TaqI TCGA 2 cut(s) 295, 326
TauI GCSGC 2 cut(s) 33, 216
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 3 cut(s) 109, 354, 487
TseI GCWGC 3 cut(s) 13, 299, 428
TspRI CASTG 3 cut(s) 109, 354, 487
XspI CTAG 3 cut(s) 17, 38, 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.