Rh5DG174300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012700
Physical Location & Seq
Forward (+)
18695079 .. 18695676
598 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG174300.1

Sequence Viewer

Length: 441 bp
ATGGCTAACGCCATAGATGACTTCGGTGTTGCTTTCGTCTTCTCCGCTGCGTCTCTTGCTGGAGTAAGGTGCACCGGTAGTGCTGCGAGGCAGGCCGATTTGCTCTTCTTGATGAGGCGTCAGGTTCTGTCTTTTCTTCTTATGATGTTTGCGGTGCTCAACCTCAGCGAAGATGGGATAGTTCCGGGCTTGGATCTAGATCGGGTGATGGTTGCGTTGGAAACGATTTTGGTTCGTCTGCTGCGCAACGGTGGGCCGCATTACTTGGCTTTAGTATGGCTGCTCACGTTACAGATTTGGATAGTGCTGAAGGGGAGGTCAACAGCGGCTGGGCTGTTGACGACGGTGTTAGTACTGGCAGAGTTGGCGGCGGAGATGGAGGTGGTGGGGGTTGGTGTGGCGACATTGCTGCTTCTTCCCGTCCTCAATCCAAAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

15.63

Weight (kDa)

5.71

Isoelectric Point (pI)

30.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 245
AciI CCGC 6 cut(s) 45, 152, 257, 326, 368, 371
AclWI GGATC 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 1 cut(s) 354
AgeI ACCGGT 1 cut(s) 74
Alw21I GWGCWC 2 cut(s) 74, 159
Alw26I GTCTC 1 cut(s) 57
Alw44I GTGCAC 1 cut(s) 70
AlwI GGATC 1 cut(s) 201
AlwNI CAGNNNCTG 2 cut(s) 127, 329
AoxI GGCC 2 cut(s) 93, 254
ApaLI GTGCAC 1 cut(s) 70
ApeKI GCWGC 5 cut(s) 47, 83, 241, 280, 409
AsiGI ACCGGT 1 cut(s) 74
AspLEI GCGC 1 cut(s) 246
AspS9I GGNCC 1 cut(s) 254
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 217
BaeGI GKGCMC 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 2 cut(s) 74, 159
BbvCI CCTCAGC 1 cut(s) 164
BbvI GCAGC 5 cut(s) 34, 70, 228, 267, 396
BccI CCATC 3 cut(s) 167, 202, 370
BcgI CGANNNNNNTGC 2 cut(s) 391, 425
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 57
BfaI CTAG 1 cut(s) 197
BisI GCNGC 8 cut(s) 48, 84, 242, 257, 281, 327, 369, 410
BlsI GCNGC 8 cut(s) 49, 85, 243, 258, 282, 328, 370, 411
BmcAI AGTACT 1 cut(s) 354
Bme1390I CCNGG 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 254
BmrFI CCNGG 1 cut(s) 186
BpiI GAAGAC 1 cut(s) 31
BpmI CTGGAG 1 cut(s) 81
Bpu10I CCTNAGC 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 118
BsaWI WCCGGW 1 cut(s) 74
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 1 cut(s) 360
Bse3DI GCAATG 1 cut(s) 404
Bse8I GATNNNNATC 1 cut(s) 198
BseJI GATNNNNATC 1 cut(s) 198
BseMI GCAATG 1 cut(s) 404
BseMII CTCAG 1 cut(s) 178
BseNI ACTGG 1 cut(s) 360
BseSI GKGCMC 1 cut(s) 74
BseXI GCAGC 5 cut(s) 34, 70, 228, 267, 396
BseYI CCCAGC 1 cut(s) 329
BshFI GGCC 2 cut(s) 95, 256
BshTI ACCGGT 1 cut(s) 74
BsiHKAI GWGCWC 2 cut(s) 74, 159
BsiSI CCGG 2 cut(s) 75, 185
BsmAI GTCTC 1 cut(s) 57
BsmBI CGTCTC 1 cut(s) 57
BsnI GGCC 2 cut(s) 95, 256
Bsp1286I GDGCHC 2 cut(s) 74, 159
Bsp143I GATC 2 cut(s) 193, 199
BspACI CCGC 6 cut(s) 45, 152, 257, 326, 368, 371
BspANI GGCC 2 cut(s) 95, 256
BspCNI CTCAG 1 cut(s) 177
BspPI GGATC 1 cut(s) 201
BspQI GCTCTTC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 404
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 1 cut(s) 360
BssAI RCCGGY 1 cut(s) 74
BssMI GATC 2 cut(s) 193, 199
BssNI GRCGYC 1 cut(s) 118
Bst4CI ACNGT 2 cut(s) 251, 346
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 1 cut(s) 118
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 1 cut(s) 164
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 2 cut(s) 196, 202
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 2 cut(s) 193, 199
BstMWI GCNNNNNNNGC 3 cut(s) 56, 92, 365
BstSCI CCNGG 1 cut(s) 184
BstSLI GKGCMC 1 cut(s) 74
BstV1I GCAGC 5 cut(s) 34, 70, 228, 267, 396
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 2 cut(s) 95, 256
Cac8I GCNNGC 1 cut(s) 93
CaiI CAGNNNCTG 2 cut(s) 127, 329
CfoI GCGC 1 cut(s) 246
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 254
CseI GACGC 2 cut(s) 39, 107
Csp6I GTAC 1 cut(s) 353
CspAI ACCGGT 1 cut(s) 74
CviJI RGCY 8 cut(s) 5, 95, 189, 256, 269, 280, 329, 334
CviKI_1 RGCY 8 cut(s) 5, 95, 189, 256, 269, 280, 329, 334
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 1 cut(s) 164
DpnI GATC 2 cut(s) 195, 201
DpnII GATC 2 cut(s) 193, 199
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 329
Esp3I CGTCTC 1 cut(s) 57
FaiI YATR 3 cut(s) 14, 143, 277
Fnu4HI GCNGC 8 cut(s) 48, 84, 242, 257, 281, 327, 369, 410
Fsp4HI GCNGC 8 cut(s) 48, 84, 242, 257, 281, 327, 369, 410
FspBI CTAG 1 cut(s) 197
FspI TGCGCA 1 cut(s) 245
GlaI GCGC 1 cut(s) 245
GluI GCNGC 8 cut(s) 48, 84, 242, 257, 281, 327, 369, 410
GsaI CCCAGC 1 cut(s) 333
GsuI CTGGAG 1 cut(s) 81
HaeIII GGCC 2 cut(s) 95, 256
HapII CCGG 2 cut(s) 75, 185
HgaI GACGC 2 cut(s) 39, 107
HhaI GCGC 1 cut(s) 246
Hin1I GRCGYC 1 cut(s) 118
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HincII GTYRAC 2 cut(s) 321, 339
HindII GTYRAC 2 cut(s) 321, 339
HpaII CCGG 2 cut(s) 75, 185
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 3 cut(s) 72, 321, 339
Hpy188III TCNNGA 2 cut(s) 109, 197
Hpy8I GTNNAC 3 cut(s) 72, 321, 339
Hpy99I CGWCG 1 cut(s) 346
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 2 cut(s) 251, 346
HpyCH4IV ACGT 1 cut(s) 287
HpyCH4V TGCA 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 92, 365
HpyF3I CTNAG 1 cut(s) 164
HpySE526I ACGT 1 cut(s) 287
Hsp92I GRCGYC 1 cut(s) 118
HspAI GCGC 1 cut(s) 244
Kzo9I GATC 2 cut(s) 193, 199
LguI GCTCTTC 1 cut(s) 110
LpnPI CCDG 7 cut(s) 45, 77, 88, 107, 198, 315, 341
Lsp1109I GCAGC 5 cut(s) 34, 70, 228, 267, 396
MaeI CTAG 1 cut(s) 197
MaeII ACGT 1 cut(s) 287
MaeIII GTNAC 1 cut(s) 288
MalI GATC 2 cut(s) 195, 201
MboI GATC 2 cut(s) 193, 199
MboII GAAGA 5 cut(s) 31, 97, 128, 182, 407
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 2 cut(s) 74, 159
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 6 cut(s) 81, 108, 173, 309, 373, 434
MspA1I CMGCKG 2 cut(s) 47, 326
MspI CCGG 2 cut(s) 75, 185
MspR9I CCNGG 1 cut(s) 186
MwoI GCNNNNNNNGC 3 cut(s) 56, 92, 365
NciI CCSGG 1 cut(s) 186
NdeII GATC 2 cut(s) 193, 199
NsbI TGCGCA 1 cut(s) 245
PciSI GCTCTTC 1 cut(s) 110
PinAI ACCGGT 1 cut(s) 74
PkrI GCNGC 8 cut(s) 49, 85, 243, 258, 282, 328, 370, 411
PspFI CCCAGC 1 cut(s) 329
PspPI GGNCC 1 cut(s) 254
PstNI CAGNNNCTG 2 cut(s) 127, 329
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
SapI GCTCTTC 1 cut(s) 110
SatI GCNGC 8 cut(s) 48, 84, 242, 257, 281, 327, 369, 410
Sau3AI GATC 2 cut(s) 193, 199
Sau96I GGNCC 1 cut(s) 254
ScaI AGTACT 1 cut(s) 354
ScrFI CCNGG 1 cut(s) 186
SduI GDGCHC 2 cut(s) 74, 159
SetI ASST 7 cut(s) 71, 126, 165, 290, 320, 384, 439
SsiI CCGC 6 cut(s) 45, 152, 257, 326, 368, 371
SspMI CTAG 1 cut(s) 197
StyD4I CCNGG 1 cut(s) 184
TaaI ACNGT 2 cut(s) 251, 346
TaiI ACGT 1 cut(s) 290
TatI WGTACW 1 cut(s) 352
TauI GCSGC 3 cut(s) 259, 329, 371
TseI GCWGC 5 cut(s) 47, 83, 241, 280, 409
VneI GTGCAC 1 cut(s) 70
XbaI TCTAGA 1 cut(s) 196
XspI CTAG 1 cut(s) 197
ZrmI AGTACT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.