Rh5DG508100

Pyruvate phosphate dikinase, PEP/pyruvate binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011364
Physical Location & Seq
Reverse (-)
79349860 .. 79352001
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG508100.1

Sequence Viewer

Length: 594 bp
ATGAAACGTCAAAATTTTAAGGGGTCAATTTCTTTTGGTGTTGGGTTCATAATTCTAGTAGTGAGTGATAAAACTCTTATTGTGCTTCAAATAATAGGAGTACCTGAACTTTGTCAAGCACATCATGGTGATGAAGCTACTGTTCCACTAAAGGAGAAGTTACTTGAATCTCGTATTGAGCTTCGCCCTCTTCTGATCGCCTCCCACAGCAGACTAAAAGATATACTGTTCCTTGATCTTGCTTTGGCTTCAGCTGTCAAAACAACATGGAAAGGGGACTCACAGATCTACATTTTTCTAACCCACCGTTCGCAGGTTATCAACCCAGTTGAAGTGCAGGGTATTATAGCTTCGGTTAATGAGCTAATTAGTATTCAGAATAAAGTCTATAAAAAGCCAACCATTCTAATTGCAACTAGAGTAACCGGGGAAGAGGAGATTCCAGATGGAGTAGTTGCTGTCCTGACACCTGATAAGCCAGATGTTCTATCTCTTGTCTCTATAAGAGCAAGAAATAGCAAGATGATATTTTGCAAAACTACTTTATTATTCTGTCTAGGAAAGTATTTCAATGGAGCCACCTATCAGCCTTAA

Protein Analysis

197

Amino Acids

21.84

Weight (kDa)

9.13

Isoelectric Point (pI)

28.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWD1_pHisD PF22973 105 - 178 3e-32 Alpha-glucan water dikinase, phosphohistidine-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 304
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 1 cut(s) 102
AfiI CCNNNNNNNGG 1 cut(s) 313
AgsI TTSAA 4 cut(s) 89, 167, 332, 571
AluBI AGCT 5 cut(s) 137, 181, 254, 350, 364
AluI AGCT 5 cut(s) 137, 181, 254, 350, 364
Alw26I GTCTC 1 cut(s) 502
ApoI RAATTY 1 cut(s) 13
AsuC2I CCSGG 1 cut(s) 427
AsuHPI GGTGA 1 cut(s) 140
BarI GAAGNNNNNNTAC 2 cut(s) 334, 366
BccI CCATC 1 cut(s) 440
BcnI CCSGG 1 cut(s) 427
BcoDI GTCTC 1 cut(s) 502
BfaI CTAG 3 cut(s) 56, 417, 557
BfuAI ACCTGC 1 cut(s) 304
BglII AGATCT 1 cut(s) 285
Bme1390I CCNGG 1 cut(s) 427
BmiI GGNNCC 1 cut(s) 577
BmrFI CCNGG 1 cut(s) 427
BmrI ACTGGG 1 cut(s) 320
BmuI ACTGGG 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 427
BsaJI CCNNGG 1 cut(s) 426
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse1I ACTGG 1 cut(s) 326
BseDI CCNNGG 1 cut(s) 426
BseLI CCNNNNNNNGG 1 cut(s) 313
BseNI ACTGG 1 cut(s) 326
BseRI GAGGAG 1 cut(s) 449
BsgI GTGCAG 1 cut(s) 356
BsiSI CCGG 1 cut(s) 426
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 1 cut(s) 502
BsmFI GGGAC 1 cut(s) 290
Bsp143I GATC 3 cut(s) 195, 235, 285
BspLI GGNNCC 1 cut(s) 577
BspMI ACCTGC 1 cut(s) 304
BsrI ACTGG 1 cut(s) 326
BssECI CCNNGG 1 cut(s) 426
BssMI GATC 3 cut(s) 195, 235, 285
Bst4CI ACNGT 3 cut(s) 142, 228, 308
Bst6I CTCTTC 2 cut(s) 195, 426
BstKTI GATC 3 cut(s) 198, 238, 288
BstMAI GTCTC 1 cut(s) 502
BstMBI GATC 3 cut(s) 195, 235, 285
BstSCI CCNGG 1 cut(s) 425
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BveI ACCTGC 1 cut(s) 304
Csp6I GTAC 1 cut(s) 101
CviAII CATG 2 cut(s) 125, 267
CviQI GTAC 1 cut(s) 101
DpnI GATC 3 cut(s) 197, 237, 287
DpnII GATC 3 cut(s) 195, 235, 285
Eam1104I CTCTTC 2 cut(s) 195, 426
EarI CTCTTC 2 cut(s) 195, 426
Eco57I CTGAAG 1 cut(s) 234
FaeI CATG 2 cut(s) 128, 270
FaiI YATR 7 cut(s) 50, 126, 224, 268, 347, 390, 503
FaqI GGGAC 1 cut(s) 290
FatI CATG 2 cut(s) 124, 266
FspBI CTAG 3 cut(s) 56, 417, 557
HapII CCGG 1 cut(s) 426
Hin1II CATG 2 cut(s) 128, 270
HinfI GANTC 3 cut(s) 167, 278, 439
HpaII CCGG 1 cut(s) 426
HphI GGTGA 1 cut(s) 140
Hpy188I TCNGA 2 cut(s) 195, 378
Hpy188III TCNNGA 2 cut(s) 443, 463
HpyCH4III ACNGT 3 cut(s) 142, 228, 308
HpyCH4IV ACGT 1 cut(s) 7
HpyCH4V TGCA 3 cut(s) 337, 413, 534
HpySE526I ACGT 1 cut(s) 7
Hsp92II CATG 2 cut(s) 128, 270
Kzo9I GATC 3 cut(s) 195, 235, 285
LmnI GCTCC 1 cut(s) 575
LpnPI CCDG 9 cut(s) 117, 299, 323, 339, 439, 456, 476, 483, 492
MaeI CTAG 3 cut(s) 56, 417, 557
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 2 cut(s) 159, 421
MalI GATC 3 cut(s) 197, 237, 287
MboI GATC 3 cut(s) 195, 235, 285
MboII GAAGA 2 cut(s) 182, 443
MflI RGATCY 1 cut(s) 285
MluCI AATT 5 cut(s) 13, 27, 51, 366, 408
MlyI GAGTC 1 cut(s) 272
MnlI CCTC 3 cut(s) 198, 211, 427
MseI TTAA 3 cut(s) 18, 357, 592
MslI CAYNNNNRTG 2 cut(s) 126, 129
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 1 cut(s) 426
MspR9I CCNGG 1 cut(s) 427
NciI CCSGG 1 cut(s) 427
NdeII GATC 3 cut(s) 195, 235, 285
NlaIII CATG 2 cut(s) 128, 270
NlaIV GGNNCC 1 cut(s) 577
PfeI GAWTC 2 cut(s) 167, 439
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PspN4I GGNNCC 1 cut(s) 577
PsuI RGATCY 1 cut(s) 285
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 1 cut(s) 102
RsaNI GTAC 1 cut(s) 101
RseI CAYNNNNRTG 2 cut(s) 126, 129
SaqAI TTAA 3 cut(s) 18, 357, 592
Sau3AI GATC 3 cut(s) 195, 235, 285
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 1 cut(s) 427
SmiMI CAYNNNNRTG 2 cut(s) 126, 129
Sse9I AATT 5 cut(s) 13, 27, 51, 366, 408
SspMI CTAG 3 cut(s) 56, 417, 557
StyD4I CCNGG 1 cut(s) 425
TaaI ACNGT 3 cut(s) 142, 228, 308
TaiI ACGT 1 cut(s) 10
TasI AATT 5 cut(s) 13, 27, 51, 366, 408
TfiI GAWTC 2 cut(s) 167, 439
Tru1I TTAA 3 cut(s) 18, 357, 592
Tru9I TTAA 3 cut(s) 18, 357, 592
TspDTI ATGAA 3 cut(s) 17, 37, 147
XapI RAATTY 1 cut(s) 13
XspI CTAG 3 cut(s) 56, 417, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.