Rh7DG428900

water dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0011364
Physical Location & Seq
Forward (+)
60651054 .. 60654893
3840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG428900.1

Sequence Viewer

Length: 885 bp
ATGGTCTCATCTCTACTCTGTAGTATTACTAATCTCTTGAATCCGGTCGGTACCACCACCGCCGATGGTGGTGATGATGGAGTGGAAGAAGATAGAGACGGTGGTGCGGCCAATGAAGTGGACACCAACGAGGCTGGGGATGGACGGAAAGGAATGATGGAATCAAATGCAGAAAGATTTGGGTTTCCATTTGATCCAGAAAATATTTATATACTGGGATGGGATCATGATTTCGCAATCACTGTTTGTGATTTGTCCCCGAAACTGCAGGAGTACCTGAACTTTGTCAAGCACATCATGAAGTTACTTGAATCTTGTATTGAGCTTCGCCTTCTTCTGATCACCTCCCACAGCAGACTAAAAGATATACTGTTCCTTGATCTTGCTTTGGCTTCAGCTGTCAAAACAACATGGAAAGGAGACTCACAGATCTACATTTTTCTAACCCACCGTTGGCAGGTTATCAACCCAGTTGAAGTGCAGGGTATTATAGCTTCGGTTAATGAGCTAATTAGTATTCAGAATAAAGTCTATAAAAAGCCAACCATTCTAATTGCAACTAGAGTAACTGGGGAAGAGGAGATTCCAGATGCAGTTGTTGCTGTCCTGACACCTGGTAAGCCAGATGTTCTATCTCATGTCTCTATAAGAGCAAGAAATAGCAAGAGGCATCACAATTGTTGGGCAAAATCTTGCAACATAAGATTTTTGAGAGGAAGGGTGCCAGCATGGATTAAAATTCCCATGTCAGTTGCCATACCATTTGGCCTTTGGAAAGGTCCTATCAGAAGTATGTTAATAATTAAGCTTTTGAAGGGGAATCCCAGTTGGATTCTTCTCATTTGTATCTGTGTCCAACCCCCTAGAAGCACTGATCAGAAGTAA

Protein Analysis

294

Amino Acids

32.78

Weight (kDa)

7.62

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWD1_pHisD PF22973 152 - 224 8.8e-33 Alpha-glucan water dikinase, phosphohistidine-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 448
Acc65I GGTACC 1 cut(s) 50
AccB1I GGYRCC 2 cut(s) 50, 721
AciI CCGC 2 cut(s) 60, 107
AclWI GGATC 2 cut(s) 188, 231
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 1 cut(s) 738
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 2 cut(s) 52, 275
AfiI CCNNNNNNNGG 2 cut(s) 453, 457
AgsI TTSAA 4 cut(s) 40, 311, 476, 814
AjnI CCWGG 1 cut(s) 613
AluBI AGCT 5 cut(s) 325, 398, 494, 508, 808
AluI AGCT 5 cut(s) 325, 398, 494, 508, 808
Alw26I GTCTC 4 cut(s) 10, 90, 414, 646
AlwI GGATC 2 cut(s) 188, 231
AoxI GGCC 2 cut(s) 108, 766
ApoI RAATTY 1 cut(s) 738
Asp718I GGTACC 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 779
AsuHPI GGTGA 2 cut(s) 83, 334
AvaII GGWCC 1 cut(s) 779
BanI GGYRCC 2 cut(s) 50, 721
BarI GAAGNNNNNNTAC 2 cut(s) 478, 510
BccI CCATC 5 cut(s) 59, 71, 134, 151, 213
BciT130I CCWGG 1 cut(s) 615
BclI TGATCA 2 cut(s) 339, 874
BcoDI GTCTC 4 cut(s) 10, 90, 414, 646
BfaI CTAG 2 cut(s) 561, 864
BfmI CTRYAG 2 cut(s) 19, 266
BfuAI ACCTGC 1 cut(s) 448
BglII AGATCT 1 cut(s) 429
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 615
Bme18I GGWCC 1 cut(s) 779
BmgT120I GGNCC 1 cut(s) 779
BmiI GGNNCC 2 cut(s) 52, 723
BmrFI CCNGG 1 cut(s) 615
BmrI ACTGGG 4 cut(s) 224, 464, 579, 819
BmsI GCATC 2 cut(s) 580, 679
BmuI ACTGGG 4 cut(s) 224, 464, 579, 819
BsaI GGTCTC 1 cut(s) 10
BsaWI WCCGGW 1 cut(s) 43
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 457
Bse1I ACTGG 4 cut(s) 219, 470, 574, 825
BseBI CCWGG 1 cut(s) 615
BseGI GGATG 2 cut(s) 145, 224
BseLI CCNNNNNNNGG 2 cut(s) 453, 457
BseNI ACTGG 4 cut(s) 219, 470, 574, 825
BseRI GAGGAG 1 cut(s) 593
BseYI CCCAGC 1 cut(s) 134
BsgI GTGCAG 1 cut(s) 500
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 2 cut(s) 110, 768
BshNI GGYRCC 2 cut(s) 50, 721
BsiEI CGRYCG 1 cut(s) 48
BsiSI CCGG 1 cut(s) 44
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 2 cut(s) 453, 457
BsmAI GTCTC 4 cut(s) 10, 90, 414, 646
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 241
BsnI GGCC 2 cut(s) 110, 768
Bso31I GGTCTC 1 cut(s) 10
Bsp143I GATC 6 cut(s) 193, 223, 339, 379, 429, 874
BspACI CCGC 2 cut(s) 60, 107
BspANI GGCC 2 cut(s) 110, 768
BspHI TCATGA 2 cut(s) 226, 297
BspLI GGNNCC 2 cut(s) 52, 723
BspMAI CTGCAG 1 cut(s) 270
BspMI ACCTGC 1 cut(s) 448
BspPI GGATC 2 cut(s) 188, 231
BspT107I GGYRCC 2 cut(s) 50, 721
BspTNI GGTCTC 1 cut(s) 10
BsrI ACTGG 4 cut(s) 219, 470, 574, 825
BssMI GATC 6 cut(s) 193, 223, 339, 379, 429, 874
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 4 cut(s) 101, 244, 372, 452
Bst6I CTCTTC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 599
BstC8I GCNNGC 1 cut(s) 726
BstF5I GGATG 2 cut(s) 145, 224
BstKTI GATC 6 cut(s) 196, 226, 342, 382, 432, 877
BstMAI GTCTC 4 cut(s) 10, 90, 414, 646
BstMBI GATC 6 cut(s) 193, 223, 339, 379, 429, 874
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 1 cut(s) 599
BstNI CCWGG 1 cut(s) 615
BstSCI CCNGG 1 cut(s) 613
BstSFI CTRYAG 2 cut(s) 19, 266
BstX2I RGATCY 1 cut(s) 429
BstXI CCANNNNNNTGG 1 cut(s) 118
BstYI RGATCY 1 cut(s) 429
BsuRI GGCC 2 cut(s) 110, 768
BtsCI GGATG 2 cut(s) 145, 224
BtsIMutI CAGTG 2 cut(s) 240, 870
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 726
CciI TCATGA 2 cut(s) 226, 297
Cfr13I GGNCC 1 cut(s) 779
CsiI ACCWGGT 1 cut(s) 613
Csp6I GTAC 2 cut(s) 51, 274
CviAII CATG 6 cut(s) 227, 298, 411, 638, 729, 745
CviQI GTAC 2 cut(s) 51, 274
DpnI GATC 6 cut(s) 195, 225, 341, 381, 431, 876
DpnII GATC 6 cut(s) 193, 223, 339, 379, 429, 874
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 779
Eco57I CTGAAG 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 779
EcoRII CCWGG 1 cut(s) 613
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 6 cut(s) 230, 301, 414, 641, 732, 748
FaqI GGGAC 1 cut(s) 241
FatI CATG 6 cut(s) 226, 297, 410, 637, 728, 744
FbaI TGATCA 2 cut(s) 339, 874
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 2 cut(s) 152, 231
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 2 cut(s) 561, 864
GluI GCNGC 1 cut(s) 108
GsaI CCCAGC 1 cut(s) 138
HaeIII GGCC 2 cut(s) 110, 768
HapII CCGG 1 cut(s) 44
Hin1II CATG 6 cut(s) 230, 301, 414, 641, 732, 748
HindIII AAGCTT 1 cut(s) 806
HinfI GANTC 7 cut(s) 40, 161, 311, 422, 583, 820, 832
HpaII CCGG 1 cut(s) 44
HphI GGTGA 2 cut(s) 83, 334
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 4 cut(s) 339, 522, 788, 879
Hpy188III TCNNGA 6 cut(s) 37, 197, 227, 298, 587, 607
Hpy8I GTNNAC 1 cut(s) 121
HpyAV CCTTC 3 cut(s) 341, 711, 808
HpyCH4III ACNGT 4 cut(s) 101, 244, 372, 452
HpyCH4V TGCA 6 cut(s) 170, 268, 481, 557, 593, 696
HpyF10VI GCNNNNNNNGC 1 cut(s) 599
Hsp92II CATG 6 cut(s) 230, 301, 414, 641, 732, 748
KpnI GGTACC 1 cut(s) 54
Ksp22I TGATCA 2 cut(s) 339, 874
Kzo9I GATC 6 cut(s) 193, 223, 339, 379, 429, 874
LweI GCATC 2 cut(s) 580, 679
MabI ACCWGGT 1 cut(s) 613
MaeI CTAG 2 cut(s) 561, 864
MaeIII GTNAC 2 cut(s) 303, 565
MalI GATC 6 cut(s) 195, 225, 341, 381, 431, 876
MboI GATC 6 cut(s) 193, 223, 339, 379, 429, 874
MboII GAAGA 5 cut(s) 98, 101, 326, 587, 827
MfeI CAATTG 1 cut(s) 676
MflI RGATCY 1 cut(s) 429
MluCI AATT 5 cut(s) 510, 552, 676, 738, 801
MlyI GAGTC 1 cut(s) 416
MmeI TCCRAC 2 cut(s) 809, 880
MnlI CCTC 5 cut(s) 124, 355, 571, 660, 707
MseI TTAA 4 cut(s) 501, 735, 797, 804
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 615
MunI CAATTG 1 cut(s) 676
MvaI CCWGG 1 cut(s) 615
MwoI GCNNNNNNNGC 1 cut(s) 599
NdeII GATC 6 cut(s) 193, 223, 339, 379, 429, 874
NlaIII CATG 6 cut(s) 230, 301, 414, 641, 732, 748
NlaIV GGNNCC 2 cut(s) 52, 723
PagI TCATGA 2 cut(s) 226, 297
PfeI GAWTC 6 cut(s) 40, 161, 311, 583, 820, 832
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 1 cut(s) 416
PpsI GAGTC 1 cut(s) 416
PpuMI RGGWCCY 1 cut(s) 779
Psp5II RGGWCCY 1 cut(s) 779
Psp6I CCWGG 1 cut(s) 613
PspFI CCCAGC 1 cut(s) 134
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 2 cut(s) 52, 723
PspPI GGNCC 1 cut(s) 779
PspPPI RGGWCCY 1 cut(s) 779
PstI CTGCAG 1 cut(s) 270
PsuI RGATCY 1 cut(s) 429
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 2 cut(s) 52, 275
RsaNI GTAC 2 cut(s) 51, 274
SaqAI TTAA 4 cut(s) 501, 735, 797, 804
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 6 cut(s) 193, 223, 339, 379, 429, 874
Sau96I GGNCC 1 cut(s) 779
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 1 cut(s) 615
SexAI ACCWGGT 1 cut(s) 613
SfaNI GCATC 2 cut(s) 580, 679
SfcI CTRYAG 2 cut(s) 19, 266
SinI GGWCC 1 cut(s) 779
Sse9I AATT 5 cut(s) 510, 552, 676, 738, 801
SsiI CCGC 2 cut(s) 60, 107
SspI AATATT 1 cut(s) 205
SspMI CTAG 2 cut(s) 561, 864
StyD4I CCNGG 1 cut(s) 613
TaaI ACNGT 4 cut(s) 101, 244, 372, 452
TasI AATT 5 cut(s) 510, 552, 676, 738, 801
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 6 cut(s) 40, 161, 311, 583, 820, 832
Tru1I TTAA 4 cut(s) 501, 735, 797, 804
Tru9I TTAA 4 cut(s) 501, 735, 797, 804
TscAI CASTG 2 cut(s) 247, 877
TspDTI ATGAA 2 cut(s) 129, 314
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 2 cut(s) 247, 877
VpaK11BI GGWCC 1 cut(s) 779
XapI RAATTY 1 cut(s) 738
XcmI CCANNNNNNNNNTGG 1 cut(s) 768
XspI CTAG 2 cut(s) 561, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.