Rh5DG541800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0015772
Physical Location & Seq
Forward (+)
83373117 .. 83375537
2421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG541800.1

Sequence Viewer

Length: 510 bp
ATGACGATTCCTGATGCAAAGAGGCTTAAGGTTTATTTGGCGAAAAGAGAGAACAAGTCGCCCCTGTGGCATGGAGTGAAGTCAGGAGTATTTGTTAAGGATGTACTAGCCATCTTGAATGAGGAAGCTGTCGGTGTGCAGGCACCCAATGAGAATGTACAAGCCCAACTTGGTGAGAATGCAGAAGCCCAGCCTCAGCCTATGCATATGCCTCCCCAGCCAGAGTCTATGCCTTCCCAAACTGCTGACCTTGTGCAGCCCCAATCTCAGTGTGACCAAGGGCCCCAAGTGGATTATATGGCTACCCAGCAGTCCCAGACTGCATCATCTGCCCCAGAAGGAGGATCCCAGCCCCAGTTCAGAGTTAGGAGGTTCAAATCCCTTGCTCATAGAAGACCCAAGCCTCAAAATGCACCCCAAGCCCACCAAGTTTATATGAATGAGTACATGATGTTGTTTTGCAGGAACCAGCTTATGTTGTTAATGCTAGACTTTTATGACTGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

19.18

Weight (kDa)

6.9

Isoelectric Point (pI)

77.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 142
AclWI GGATC 2 cut(s) 339, 352
AfaI GTAC 3 cut(s) 105, 159, 446
AfiI CCNNNNNNNGG 1 cut(s) 341
AflII CTTAAG 1 cut(s) 26
AgsI TTSAA 2 cut(s) 118, 376
AluBI AGCT 2 cut(s) 128, 472
AluI AGCT 2 cut(s) 128, 472
AlwI GGATC 2 cut(s) 339, 352
AoxI GGCC 1 cut(s) 281
ApaI GGGCCC 1 cut(s) 285
ApeKI GCWGC 1 cut(s) 256
AspS9I GGNCC 2 cut(s) 281, 282
AsuHPI GGTGA 1 cut(s) 185
BaeGI GKGCMC 1 cut(s) 285
BamHI GGATCC 1 cut(s) 344
BanI GGYRCC 1 cut(s) 142
BanII GRGCYC 1 cut(s) 285
BbsI GAAGAC 1 cut(s) 400
BbvCI CCTCAGC 1 cut(s) 195
BbvI GCAGC 1 cut(s) 268
BccI CCATC 1 cut(s) 119
BfaI CTAG 2 cut(s) 107, 488
BfrI CTTAAG 1 cut(s) 26
BglI GCCNNNNNGGC 1 cut(s) 67
BisI GCNGC 1 cut(s) 257
BlsI GCNGC 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 281, 282
BmiI GGNNCC 5 cut(s) 144, 283, 284, 346, 467
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 2 cut(s) 4, 332
BmuI ACTGGG 1 cut(s) 349
BpiI GAAGAC 1 cut(s) 400
Bpu10I CCTNAGC 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 1 cut(s) 341
Bse1I ACTGG 2 cut(s) 355, 506
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 341
BseMII CTCAG 2 cut(s) 209, 281
BseNI ACTGG 2 cut(s) 355, 506
BseSI GKGCMC 1 cut(s) 285
BseXI GCAGC 1 cut(s) 268
BseYI CCCAGC 4 cut(s) 189, 216, 306, 348
BsgI GTGCAG 2 cut(s) 158, 275
BshFI GGCC 1 cut(s) 283
BshNI GGYRCC 1 cut(s) 142
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 1 cut(s) 184
BsnI GGCC 1 cut(s) 283
Bsp120I GGGCCC 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 285
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 1 cut(s) 344
BspANI GGCC 1 cut(s) 283
BspCNI CTCAG 2 cut(s) 208, 280
BspLI GGNNCC 5 cut(s) 144, 283, 284, 346, 467
BspPI GGATC 2 cut(s) 339, 352
BspT107I GGYRCC 1 cut(s) 142
BspTI CTTAAG 1 cut(s) 26
BsrGI TGTACA 1 cut(s) 157
BsrI ACTGG 2 cut(s) 355, 506
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 277
BstAFI CTTAAG 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 329
BstAUI TGTACA 1 cut(s) 157
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 2 cut(s) 195, 267
BstF5I GGATG 1 cut(s) 106
BstKTI GATC 1 cut(s) 347
BstMBI GATC 1 cut(s) 344
BstMWI GCNNNNNNNGC 4 cut(s) 67, 217, 329, 419
BstSLI GKGCMC 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 268
BstV2I GAAGAC 1 cut(s) 400
BstX2I RGATCY 1 cut(s) 344
BstYI RGATCY 1 cut(s) 344
BsuRI GGCC 1 cut(s) 283
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 1 cut(s) 141
Cfr13I GGNCC 2 cut(s) 281, 282
Csp6I GTAC 3 cut(s) 104, 158, 445
CviAII CATG 2 cut(s) 71, 448
CviQI GTAC 3 cut(s) 104, 158, 445
DdeI CTNAG 2 cut(s) 195, 267
DpnI GATC 1 cut(s) 346
DpnII GATC 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 285
EcoO109I RGGNCCY 2 cut(s) 281, 282
EcoT14I CCWWGG 1 cut(s) 277
EcoT22I ATGCAT 1 cut(s) 207
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 2 cut(s) 74, 451
FalI AAGNNNNNCTT 2 cut(s) 153, 185
FaqI GGGAC 1 cut(s) 298
FatI CATG 2 cut(s) 70, 447
FauNDI CATATG 1 cut(s) 207
Fnu4HI GCNGC 1 cut(s) 257
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 1 cut(s) 257
FspBI CTAG 2 cut(s) 107, 488
GluI GCNGC 1 cut(s) 257
GsaI CCCAGC 4 cut(s) 193, 220, 310, 352
HaeIII GGCC 1 cut(s) 283
Hin1II CATG 2 cut(s) 74, 451
HinfI GANTC 2 cut(s) 7, 224
HphI GGTGA 1 cut(s) 185
Hpy188I TCNGA 2 cut(s) 362, 509
Hpy188III TCNNGA 3 cut(s) 11, 84, 115
HpyAV CCTTC 2 cut(s) 243, 332
HpyCH4V TGCA 8 cut(s) 17, 139, 182, 205, 256, 323, 413, 462
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 217, 329, 419
HpyF3I CTNAG 2 cut(s) 195, 267
Hsp92II CATG 2 cut(s) 74, 451
Kzo9I GATC 1 cut(s) 344
Lsp1109I GCAGC 1 cut(s) 268
LweI GCATC 2 cut(s) 4, 332
MaeI CTAG 2 cut(s) 107, 488
MaeIII GTNAC 1 cut(s) 272
MalI GATC 1 cut(s) 346
MboI GATC 1 cut(s) 344
MboII GAAGA 1 cut(s) 405
MflI RGATCY 1 cut(s) 344
MhlI GDGCHC 1 cut(s) 285
MlyI GAGTC 1 cut(s) 233
MnlI CCTC 7 cut(s) 15, 115, 204, 222, 335, 363, 414
Mph1103I ATGCAT 1 cut(s) 207
MseI TTAA 3 cut(s) 27, 96, 482
MspCI CTTAAG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 184
MwoI GCNNNNNNNGC 4 cut(s) 67, 217, 329, 419
NdeI CATATG 1 cut(s) 207
NdeII GATC 1 cut(s) 344
NlaIII CATG 2 cut(s) 74, 451
NlaIV GGNNCC 5 cut(s) 144, 283, 284, 346, 467
NmuCI GTSAC 1 cut(s) 272
NsiI ATGCAT 1 cut(s) 207
PctI GAATGC 1 cut(s) 184
PfeI GAWTC 1 cut(s) 7
PkrI GCNGC 1 cut(s) 258
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
PspFI CCCAGC 4 cut(s) 189, 216, 306, 348
PspN4I GGNNCC 5 cut(s) 144, 283, 284, 346, 467
PspOMI GGGCCC 1 cut(s) 281
PspPI GGNCC 2 cut(s) 281, 282
PsuI RGATCY 1 cut(s) 344
RsaI GTAC 3 cut(s) 105, 159, 446
RsaNI GTAC 3 cut(s) 104, 158, 445
SaqAI TTAA 3 cut(s) 27, 96, 482
SatI GCNGC 1 cut(s) 257
Sau3AI GATC 1 cut(s) 344
Sau96I GGNCC 2 cut(s) 281, 282
SchI GAGTC 1 cut(s) 233
SduI GDGCHC 1 cut(s) 285
SetI ASST 5 cut(s) 33, 130, 252, 374, 474
SfaNI GCATC 2 cut(s) 4, 332
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
SspMI CTAG 2 cut(s) 107, 488
StyI CCWWGG 1 cut(s) 277
TatI WGTACW 3 cut(s) 103, 157, 444
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 3 cut(s) 27, 96, 482
Tru9I TTAA 3 cut(s) 27, 96, 482
TscAI CASTG 1 cut(s) 275
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 1 cut(s) 256
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 1 cut(s) 452
TspRI CASTG 1 cut(s) 275
Vha464I CTTAAG 1 cut(s) 26
XspI CTAG 2 cut(s) 107, 488
Zsp2I ATGCAT 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.