Rh6CG305000

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0010626
Physical Location & Seq
Forward (+)
48588873 .. 48594788
5916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG305000.1

Sequence Viewer

Length: 561 bp
ATGTCATCATCTTCAAGAATCCTTCTTTGCTTGTTCTTAGTGTTGCTCACTACTAGTCTACAGCACACAGAAGCAACCTTTGAGTCTTCACTGAGTGTTGACGAAGGACCTTGGGGTAATGGAATTGATGAGCTTGTTGTTCATAATCTAGTAGAGAGACATTTCATACGGTCACATGTATTCTCTTCAAAGCCAGGAGGTTGTGCAGCATTTCTCTCAAACTACAATGCAAACTCAGCTGCAACGGTAACTTTCAATGACCGAAACTATGAGCTCCTTGCTTGGTCCATCAGCGTCCTTCCAGACTGTGAAAATGTTGTATTCGACACTACCAAAGTGGGAGTTCAGACATCCCAAAAACAATTTTTTCCCACTAACTCTGAGTTGTGGTCATGGGAAACCTTCAGTGAAGACATTGCTTCATGGACTGGTGATACAACTCTCACAGTCATTGGTTTCTTAGATCAGTTGAACATCACTAGAGACTCTAGTGATTATCTATGGTACACCATCAGATCATTAATAGTTGGAGACAGCGTCAGGAAACCCTTGAAGGTGTAA

Protein Analysis

186

Amino Acids

20.83

Weight (kDa)

4.75

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GHD PF17834 58 - 113 9e-23 Beta-sandwich domain in beta galactosidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 388
AdeI CACNNNGTG 1 cut(s) 95
AfaI GTAC 1 cut(s) 506
AflIII ACRYGT 1 cut(s) 175
AgsI TTSAA 5 cut(s) 15, 189, 256, 472, 553
AhlI ACTAGT 1 cut(s) 53
AjnI CCWGG 1 cut(s) 193
AluBI AGCT 3 cut(s) 133, 239, 274
AluI AGCT 3 cut(s) 133, 239, 274
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 3 cut(s) 151, 477, 525
ApeKI GCWGC 2 cut(s) 206, 239
AseI ATTAAT 1 cut(s) 521
AspS9I GGNCC 2 cut(s) 107, 285
AsuHPI GGTGA 1 cut(s) 443
AvaII GGWCC 2 cut(s) 107, 285
BanII GRGCYC 1 cut(s) 276
BbsI GAAGAC 2 cut(s) 78, 417
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 2 cut(s) 218, 226
BccI CCATC 2 cut(s) 296, 518
BciT130I CCWGG 1 cut(s) 195
BcoDI GTCTC 3 cut(s) 151, 477, 525
BcuI ACTAGT 1 cut(s) 53
BfaI CTAG 4 cut(s) 54, 149, 480, 489
BfmI CTRYAG 1 cut(s) 59
BisI GCNGC 2 cut(s) 207, 240
BlsI GCNGC 2 cut(s) 208, 241
Bme1390I CCNGG 1 cut(s) 195
Bme18I GGWCC 2 cut(s) 107, 285
BmgT120I GGNCC 2 cut(s) 107, 285
BmrFI CCNGG 1 cut(s) 195
BpiI GAAGAC 2 cut(s) 78, 417
BsaJI CCNNGG 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 522, 552
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 110
BseGI GGATG 1 cut(s) 350
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 3 cut(s) 83, 249, 372
BseNI ACTGG 1 cut(s) 433
BseXI GCAGC 2 cut(s) 218, 226
BsgI GTGCAG 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 276
BsmAI GTCTC 3 cut(s) 151, 477, 525
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 2 cut(s) 463, 515
BspCNI CTCAG 3 cut(s) 84, 248, 373
BsrDI GCAATG 1 cut(s) 414
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 2 cut(s) 463, 515
BssT1I CCWWGG 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 4 cut(s) 171, 247, 308, 448
Bst6I CTCTTC 1 cut(s) 190
BstDEI CTNAG 5 cut(s) 37, 92, 235, 381, 460
BstF5I GGATG 1 cut(s) 350
BstKTI GATC 2 cut(s) 466, 518
BstMAI GTCTC 3 cut(s) 151, 477, 525
BstMBI GATC 2 cut(s) 463, 515
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 193
BstSFI CTRYAG 1 cut(s) 59
BstV1I GCAGC 2 cut(s) 218, 226
BstV2I GAAGAC 2 cut(s) 78, 417
BtsCI GGATG 1 cut(s) 350
BtsIMutI CAGTG 2 cut(s) 89, 412
Cfr13I GGNCC 2 cut(s) 107, 285
CseI GACGC 2 cut(s) 283, 526
Csp6I GTAC 1 cut(s) 505
CviAII CATG 3 cut(s) 176, 393, 423
CviJI RGCY 4 cut(s) 133, 193, 239, 274
CviKI_1 RGCY 4 cut(s) 133, 193, 239, 274
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 5 cut(s) 37, 92, 235, 381, 460
DpnI GATC 2 cut(s) 465, 517
DpnII GATC 2 cut(s) 463, 515
DraIII CACNNNGTG 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 110
Eco24I GRGCYC 1 cut(s) 276
Eco47I GGWCC 2 cut(s) 107, 285
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 388
EcoICRI GAGCTC 1 cut(s) 274
EcoO109I RGGNCCY 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 110
EcoT38I GRGCYC 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 110
FaeI CATG 3 cut(s) 179, 396, 426
FaiI YATR 7 cut(s) 144, 167, 177, 270, 394, 424, 502
FatI CATG 3 cut(s) 175, 392, 422
FblI GTMKAC 1 cut(s) 58
Fnu4HI GCNGC 2 cut(s) 207, 240
FokI GGATG 1 cut(s) 337
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 2 cut(s) 207, 240
FspBI CTAG 4 cut(s) 54, 149, 480, 489
GluI GCNGC 2 cut(s) 207, 240
HgaI GACGC 2 cut(s) 283, 526
Hin1II CATG 3 cut(s) 179, 396, 426
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 3 cut(s) 18, 83, 485
HphI GGTGA 1 cut(s) 443
Hpy166II GTNNAC 3 cut(s) 59, 100, 507
Hpy188I TCNGA 3 cut(s) 348, 382, 515
Hpy188III TCNNGA 3 cut(s) 15, 302, 541
Hpy8I GTNNAC 3 cut(s) 59, 100, 507
HpyAV CCTTC 5 cut(s) 32, 98, 308, 412, 547
HpyCH4III ACNGT 4 cut(s) 171, 247, 308, 448
HpyCH4V TGCA 3 cut(s) 206, 230, 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 5 cut(s) 37, 92, 235, 381, 460
Hsp92II CATG 3 cut(s) 179, 396, 426
Kzo9I GATC 2 cut(s) 463, 515
LmnI GCTCC 1 cut(s) 279
LpnPI CCDG 5 cut(s) 180, 207, 315, 414, 526
Lsp1109I GCAGC 2 cut(s) 218, 226
MaeI CTAG 4 cut(s) 54, 149, 480, 489
MaeIII GTNAC 2 cut(s) 171, 247
MalI GATC 2 cut(s) 465, 517
MboI GATC 2 cut(s) 463, 515
MboII GAAGA 4 cut(s) 3, 78, 177, 422
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 2 cut(s) 123, 362
MlyI GAGTC 2 cut(s) 92, 479
MmeI TCCRAC 1 cut(s) 508
MnlI CCTC 1 cut(s) 191
MseI TTAA 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 195
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 1 cut(s) 236
NdeII GATC 2 cut(s) 463, 515
NlaIII CATG 3 cut(s) 179, 396, 426
NmuCI GTSAC 1 cut(s) 171
NspI RCATGY 1 cut(s) 179
PciI ACATGT 1 cut(s) 175
PfeI GAWTC 1 cut(s) 18
PflFI GACNNNGTC 1 cut(s) 536
PkrI GCNGC 2 cut(s) 208, 241
PleI GAGTC 2 cut(s) 91, 479
PpsI GAGTC 2 cut(s) 91, 479
PpuMI RGGWCCY 1 cut(s) 107
PscI ACATGT 1 cut(s) 175
PshBI ATTAAT 1 cut(s) 521
Psp124BI GAGCTC 1 cut(s) 276
Psp5II RGGWCCY 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspPI GGNCC 2 cut(s) 107, 285
PspPPI RGGWCCY 1 cut(s) 107
PsyI GACNNNGTC 1 cut(s) 536
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 1 cut(s) 521
SatI GCNGC 2 cut(s) 207, 240
Sau3AI GATC 2 cut(s) 463, 515
Sau96I GGNCC 2 cut(s) 107, 285
SchI GAGTC 2 cut(s) 92, 479
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 1 cut(s) 276
SetI ASST 8 cut(s) 80, 112, 135, 202, 241, 276, 404, 558
SfcI CTRYAG 1 cut(s) 59
SinI GGWCC 2 cut(s) 107, 285
SpeI ACTAGT 1 cut(s) 53
Sse9I AATT 2 cut(s) 123, 362
SspMI CTAG 4 cut(s) 54, 149, 480, 489
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 1 cut(s) 193
StyI CCWWGG 1 cut(s) 110
TaaI ACNGT 4 cut(s) 171, 247, 308, 448
TaqI TCGA 1 cut(s) 324
TaqII GACCGA 1 cut(s) 276
TasI AATT 2 cut(s) 123, 362
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 1 cut(s) 521
Tru9I TTAA 1 cut(s) 521
TscAI CASTG 2 cut(s) 96, 412
TseFI GTSAC 1 cut(s) 171
TseI GCWGC 2 cut(s) 206, 239
Tsp45I GTSAC 1 cut(s) 171
TspDTI ATGAA 3 cut(s) 131, 154, 411
TspRI CASTG 2 cut(s) 96, 412
Tth111I GACNNNGTC 1 cut(s) 536
VpaK11BI GGWCC 2 cut(s) 107, 285
VspI ATTAAT 1 cut(s) 521
XceI RCATGY 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 58
XspI CTAG 4 cut(s) 54, 149, 480, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.