Rh6DG518000

TAF RNA Polymerase I subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002593
Physical Location & Seq
Forward (+)
66918007 .. 66924922
6916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG518000.1

Sequence Viewer

Length: 1440 bp
ATGAAGGTTTTTGCTTTTGTGCAGTACAGACATGCTGTTGAACTTGAGCGCATCTCGCTCTCCCTTGCAAAAGGGAATCTTATGGAGGCTGAAGCCGGTGCTTTCGGTCTCATGCAAGCACATGGTTTTCGAAATGACCCTCTTCGAAATATGATTGTCGGATTGACATTCCAGCAATTGTGGTATTCCAAGCTCCCAAAAGAGATGCAGTGGAGGGAGGCTGACCAGTTTTACACTAGGGAATCAAATGAACGAGTTGCAGGCTCAGAGGGGCATGGCTCTATTAACAGTAACGAGGCTGGTAGCGCCATTCATTGTGATTCAGACACCTCTGTCATCAATGATAAAGTAGAGAATGTACCACCTATTGTTGCTGTTGATGCTGACAGTGGCTTACATAGAGAGATCTCTGTGGAGGTTGTTGACATGGAAGTAGAAATTTCTCCACCGAAATTTGAGACCCAAAATTTCAATGCGGACTCTGCTGAAAATTCAGAAGATGAAGCTGCTTTATCTGATCATGGTGGTCAAATGCAGTATGCTCCCATTTTCTCTGAACTTGAGGGGTTGGAGTCATTGTTACTGCCCATACGATTGCCAGAGTCTCTCGATAATCATGAAGATAAGAATATTTTTAATGACTATTACAAGGATGCAGTGAAGTACTTACGACATGCTCTTCACTCTTCACCCCCGGTATTGGTAGCCTTACATCCTTTGATACAGCTGTTGCTGATTGGAGGTCAAGTTAAAGAGGCCCTAGATGAGATTGAATATTGTTGTAATTTTTCAAACACAGCGCTGCCTACTCGATTAAGGTCTAGTCTTCTGCAGCAATTTGATTCTAATAACAAACCTGTGCTTTCCACTTGTCTGGAGGAAAACTTAAGGAAGGATCCTACATGTTGCGATTCATTGGAAAAGCTTGTTCTGTTGCATCAAAATGATAATTATAGTCCTGAGTCCCTGCTTGAAATGATAGCTTTGCATTTAGATGCCACCAATGCTGAATACAACATATGGAGAGAGTATGCTATGTGTTTCCTGAAGCTTTCTCAGTATGAAGAGGACAGGATGTCGGTGTGTCTGAATGGAAATGAAGGTGGACATAAACCACGGTACTCTGTTTCTTTCAATAAGACCCCGAAAATATTTATAAAGGGACAATCTGGAAAGAATTGGAAATTGCGCTGTAGATGGTGGTCTACGCGGCACTTCAGCCATGATATTCTTGCATCAGAGATTGCAGCAGGTGATTTGGAGCTCTTAACTTACAAAGCAGCGAGTGCAGTGCATATGTATGGATCAGAGTTCTATTACGTTGTGGATGCTCTTTGCTGCTTAGAGAAAGAAAGTGAGAAGGACTTGCTCTGTTTTTTGCAAATGCACATTCGAAATTCTGTTCGAATTTATTCAAATTCTCGACAGAGAACTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAF1_subA

Protein Analysis

479

Amino Acids

54.45

Weight (kDa)

5.16

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAF1_subA PF14929 1 - 443 2e-63 TAF RNA Polymerase I subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1157
AarI CACCTGC 1 cut(s) 1241
AasI GACNNNNNNGTC 1 cut(s) 332
Acc36I ACCTGC 1 cut(s) 1241
AccI GTMKAC 1 cut(s) 1205
AccII CGCG 1 cut(s) 1210
AciI CCGC 2 cut(s) 476, 1210
AclWI GGATC 3 cut(s) 890, 903, 1312
AcsI RAATTY 7 cut(s) 438, 452, 466, 490, 1396, 1407, 1417
AcuI CTGAAG 3 cut(s) 111, 1067, 1201
AfaI GTAC 4 cut(s) 26, 360, 665, 1121
AfeI AGCGCT 1 cut(s) 801
AfiI CCNNNNNNNGG 1 cut(s) 700
AflII CTTAAG 1 cut(s) 886
AflIII ACRYGT 1 cut(s) 902
AgsI TTSAA 7 cut(s) 41, 472, 773, 792, 974, 1135, 1416
AhdI GACNNNNNGTC 1 cut(s) 1075
AluBI AGCT 7 cut(s) 193, 506, 727, 925, 983, 1051, 1264
AluI AGCT 7 cut(s) 193, 506, 727, 925, 983, 1051, 1264
Alw21I GWGCWC 1 cut(s) 1266
Alw26I GTCTC 3 cut(s) 113, 452, 609
AlwI GGATC 3 cut(s) 890, 903, 1312
Aor51HI AGCGCT 1 cut(s) 801
AoxI GGCC 1 cut(s) 756
ApeKI GCWGC 6 cut(s) 506, 802, 832, 1247, 1280, 1338
ApoI RAATTY 7 cut(s) 438, 452, 466, 490, 1396, 1407, 1417
Asp700I GAANNNNTTC 1 cut(s) 1411
AspLEI GCGC 4 cut(s) 51, 308, 802, 1191
AspS9I GGNCC 1 cut(s) 757
AsuC2I CCSGG 1 cut(s) 695
AsuHPI GGTGA 2 cut(s) 681, 1265
AsuII TTCGAA 4 cut(s) 130, 145, 1393, 1405
BaeI ACNNNNGTAYC 2 cut(s) 713, 746
BamHI GGATCC 1 cut(s) 895
BanII GRGCYC 1 cut(s) 1266
BbsI GAAGAC 1 cut(s) 818
Bbv12I GWGCWC 1 cut(s) 1266
BbvI GCAGC 6 cut(s) 493, 789, 844, 1259, 1292, 1325
BccI CCATC 1 cut(s) 1191
BcgI CGANNNNNNTGC 2 cut(s) 1273, 1307
BclI TGATCA 1 cut(s) 517
BcnI CCSGG 1 cut(s) 695
BcoDI GTCTC 3 cut(s) 113, 452, 609
BfaI CTAG 3 cut(s) 237, 761, 822
BfmI CTRYAG 2 cut(s) 830, 1192
BfoI RGCGCY 2 cut(s) 309, 803
BfrI CTTAAG 1 cut(s) 886
BfuAI ACCTGC 1 cut(s) 1241
BglII AGATCT 1 cut(s) 405
BisI GCNGC 7 cut(s) 507, 803, 833, 1211, 1248, 1281, 1339
BlsI GCNGC 7 cut(s) 508, 804, 834, 1212, 1249, 1282, 1340
BmcAI AGTACT 1 cut(s) 665
Bme1390I CCNGG 1 cut(s) 695
BmeRI GACNNNNNGTC 1 cut(s) 1075
BmgT120I GGNCC 1 cut(s) 757
BmiI GGNNCC 1 cut(s) 897
BmrFI CCNGG 1 cut(s) 695
BmsI GCATC 8 cut(s) 60, 195, 370, 643, 946, 985, 1244, 1318
BpiI GAAGAC 1 cut(s) 818
BplI GAGNNNNNCTC 2 cut(s) 38, 70
BpmI CTGGAG 1 cut(s) 896
Bpu14I TTCGAA 4 cut(s) 130, 145, 1393, 1405
BpuEI CTTGAG 2 cut(s) 65, 581
BpuMI CCSGG 1 cut(s) 695
BsaI GGTCTC 2 cut(s) 113, 452
BsaJI CCNNGG 2 cut(s) 693, 1115
BsaXI ACNNNNNCTCC 4 cut(s) 563, 593, 732, 762
Bsc4I CCNNNNNNNGG 1 cut(s) 700
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 1 cut(s) 226
BseDI CCNNGG 2 cut(s) 693, 1115
BseGI GGATG 4 cut(s) 658, 712, 1080, 1333
BseLI CCNNNNNNNGG 1 cut(s) 700
BseMII CTCAG 3 cut(s) 279, 951, 1070
BseNI ACTGG 1 cut(s) 226
BseXI GCAGC 6 cut(s) 493, 789, 844, 1259, 1292, 1325
BsgI GTGCAG 2 cut(s) 41, 1308
Bsh1236I CGCG 1 cut(s) 1210
BshFI GGCC 1 cut(s) 758
BsiHKAI GWGCWC 1 cut(s) 1266
BsiSI CCGG 2 cut(s) 96, 695
BslFI GGGAC 2 cut(s) 949, 1176
BslI CCNNNNNNNGG 1 cut(s) 700
BsmAI GTCTC 3 cut(s) 113, 452, 609
BsmFI GGGAC 2 cut(s) 949, 1176
BsnI GGCC 1 cut(s) 758
Bso31I GGTCTC 2 cut(s) 113, 452
Bsp119I TTCGAA 4 cut(s) 130, 145, 1393, 1405
Bsp1286I GDGCHC 1 cut(s) 1266
Bsp143I GATC 4 cut(s) 405, 517, 895, 1304
BspACI CCGC 2 cut(s) 476, 1210
BspANI GGCC 1 cut(s) 758
BspCNI CTCAG 3 cut(s) 278, 952, 1069
BspFNI CGCG 1 cut(s) 1210
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 1 cut(s) 897
BspMAI CTGCAG 1 cut(s) 834
BspMI ACCTGC 1 cut(s) 1241
BspPI GGATC 3 cut(s) 890, 903, 1312
BspQI GCTCTTC 1 cut(s) 684
BspT104I TTCGAA 4 cut(s) 130, 145, 1393, 1405
BspTI CTTAAG 1 cut(s) 886
BspTNI GGTCTC 2 cut(s) 113, 452
BsrFI RCCGGY 1 cut(s) 95
BsrI ACTGG 1 cut(s) 226
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 2 cut(s) 693, 1115
BssMI GATC 4 cut(s) 405, 517, 895, 1304
Bst4CI ACNGT 3 cut(s) 290, 389, 1119
Bst6I CTCTTC 4 cut(s) 147, 684, 691, 1059
BstAFI CTTAAG 1 cut(s) 886
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstBI TTCGAA 4 cut(s) 130, 145, 1393, 1405
BstC8I GCNNGC 2 cut(s) 117, 262
BstDEI CTNAG 4 cut(s) 265, 960, 1056, 1342
BstDSI CCRYGG 1 cut(s) 1115
BstF5I GGATG 4 cut(s) 658, 712, 1080, 1333
BstFNI CGCG 1 cut(s) 1210
BstH2I RGCGCY 2 cut(s) 309, 803
BstHHI GCGC 4 cut(s) 51, 308, 802, 1191
BstKTI GATC 4 cut(s) 408, 520, 898, 1307
BstMAI GTCTC 3 cut(s) 113, 452, 609
BstMBI GATC 4 cut(s) 405, 517, 895, 1304
BstMWI GCNNNNNNNGC 6 cut(s) 55, 305, 380, 482, 1004, 1286
BstNSI RCATGY 3 cut(s) 35, 677, 906
BstSCI CCNGG 1 cut(s) 693
BstSFI CTRYAG 2 cut(s) 830, 1192
BstUI CGCG 1 cut(s) 1210
BstV1I GCAGC 6 cut(s) 493, 789, 844, 1259, 1292, 1325
BstV2I GAAGAC 1 cut(s) 818
BstX2I RGATCY 2 cut(s) 405, 895
BstXI CCANNNNNNTGG 1 cut(s) 874
BstYI RGATCY 2 cut(s) 405, 895
BsuRI GGCC 1 cut(s) 758
BtgI CCRYGG 1 cut(s) 1115
BtsCI GGATG 4 cut(s) 658, 712, 1080, 1333
BtsI GCAGTG 3 cut(s) 215, 663, 1296
BtsIMutI CAGTG 4 cut(s) 215, 394, 663, 1296
BveI ACCTGC 1 cut(s) 1241
Cac8I GCNNGC 2 cut(s) 117, 262
CciI TCATGA 1 cut(s) 616
CfoI GCGC 4 cut(s) 51, 308, 802, 1191
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 757
Csp6I GTAC 4 cut(s) 25, 359, 664, 1120
CviQI GTAC 4 cut(s) 25, 359, 664, 1120
DdeI CTNAG 4 cut(s) 265, 960, 1056, 1342
DpnI GATC 4 cut(s) 407, 519, 897, 1306
DpnII GATC 4 cut(s) 405, 517, 895, 1304
DrdI GACNNNNNNGTC 1 cut(s) 332
DriI GACNNNNNGTC 1 cut(s) 1075
DseDI GACNNNNNNGTC 1 cut(s) 332
Eam1104I CTCTTC 4 cut(s) 147, 684, 691, 1059
Eam1105I GACNNNNNGTC 1 cut(s) 1075
EarI CTCTTC 4 cut(s) 147, 684, 691, 1059
Ecl136II GAGCTC 1 cut(s) 1264
Eco24I GRGCYC 1 cut(s) 1266
Eco31I GGTCTC 2 cut(s) 113, 452
Eco47III AGCGCT 1 cut(s) 801
Eco53kI GAGCTC 1 cut(s) 1264
Eco57I CTGAAG 3 cut(s) 111, 1067, 1201
EcoICRI GAGCTC 1 cut(s) 1264
EcoO109I RGGNCCY 1 cut(s) 757
EcoT38I GRGCYC 1 cut(s) 1266
FalI AAGNNNNNCTT 2 cut(s) 63, 95
FaqI GGGAC 2 cut(s) 949, 1176
FauNDI CATATG 2 cut(s) 1019, 1296
FbaI TGATCA 1 cut(s) 517
FblI GTMKAC 1 cut(s) 1205
Fnu4HI GCNGC 7 cut(s) 507, 803, 833, 1211, 1248, 1281, 1339
FokI GGATG 4 cut(s) 665, 699, 1087, 1340
FriOI GRGCYC 1 cut(s) 1266
Fsp4HI GCNGC 7 cut(s) 507, 803, 833, 1211, 1248, 1281, 1339
FspBI CTAG 3 cut(s) 237, 761, 822
GlaI GCGC 4 cut(s) 50, 307, 801, 1190
GluI GCNGC 7 cut(s) 507, 803, 833, 1211, 1248, 1281, 1339
GsuI CTGGAG 1 cut(s) 896
HaeII RGCGCY 2 cut(s) 309, 803
HaeIII GGCC 1 cut(s) 758
HapII CCGG 2 cut(s) 96, 695
HhaI GCGC 4 cut(s) 51, 308, 802, 1191
Hin6I GCGC 4 cut(s) 49, 306, 800, 1189
HinP1I GCGC 4 cut(s) 49, 306, 800, 1189
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 2 cut(s) 923, 1049
HinfI GANTC 9 cut(s) 76, 242, 320, 479, 572, 602, 842, 911, 962
HpaII CCGG 2 cut(s) 96, 695
HphI GGTGA 2 cut(s) 681, 1265
Hpy166II GTNNAC 3 cut(s) 424, 1106, 1206
Hpy188I TCNGA 9 cut(s) 161, 268, 325, 496, 517, 556, 1089, 1240, 1309
Hpy188III TCNNGA 7 cut(s) 608, 617, 875, 959, 1045, 1170, 1422
Hpy8I GTNNAC 3 cut(s) 424, 1106, 1206
HpyAV CCTTC 3 cut(s) 886, 1094, 1354
HpyCH4III ACNGT 3 cut(s) 290, 389, 1119
HpyCH4IV ACGT 1 cut(s) 1320
HpyF10VI GCNNNNNNNGC 6 cut(s) 55, 305, 380, 482, 1004, 1286
HpyF3I CTNAG 4 cut(s) 265, 960, 1056, 1342
HpySE526I ACGT 1 cut(s) 1320
HspAI GCGC 4 cut(s) 49, 306, 800, 1189
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 4 cut(s) 405, 517, 895, 1304
LguI GCTCTTC 1 cut(s) 684
LmnI GCTCC 3 cut(s) 198, 547, 1261
Lsp1109I GCAGC 6 cut(s) 493, 789, 844, 1259, 1292, 1325
LweI GCATC 8 cut(s) 60, 195, 370, 643, 946, 985, 1244, 1318
MaeI CTAG 3 cut(s) 237, 761, 822
MaeII ACGT 1 cut(s) 1320
MaeIII GTNAC 2 cut(s) 290, 579
MalI GATC 4 cut(s) 407, 519, 897, 1306
MboI GATC 4 cut(s) 405, 517, 895, 1304
MboII GAAGA 7 cut(s) 134, 509, 632, 671, 678, 818, 1076
MfeI CAATTG 1 cut(s) 176
MflI RGATCY 2 cut(s) 405, 895
MhlI GDGCHC 1 cut(s) 1266
MlyI GAGTC 4 cut(s) 473, 581, 611, 971
MmeI TCCRAC 2 cut(s) 139, 549
MroXI GAANNNNTTC 1 cut(s) 1411
MseI TTAA 6 cut(s) 285, 636, 750, 815, 887, 1268
MslI CAYNNNNRTG 3 cut(s) 942, 993, 1299
MspA1I CMGCKG 1 cut(s) 727
MspCI CTTAAG 1 cut(s) 886
MspI CCGG 2 cut(s) 96, 695
MspR9I CCNGG 1 cut(s) 695
MunI CAATTG 1 cut(s) 176
MvnI CGCG 1 cut(s) 1210
MwoI GCNNNNNNNGC 6 cut(s) 55, 305, 380, 482, 1004, 1286
NciI CCSGG 1 cut(s) 695
NdeI CATATG 2 cut(s) 1019, 1296
NdeII GATC 4 cut(s) 405, 517, 895, 1304
NlaIV GGNNCC 1 cut(s) 897
NspI RCATGY 3 cut(s) 35, 677, 906
NspV TTCGAA 4 cut(s) 130, 145, 1393, 1405
PagI TCATGA 1 cut(s) 616
PaqCI CACCTGC 1 cut(s) 1241
PciI ACATGT 1 cut(s) 902
PciSI GCTCTTC 1 cut(s) 684
PdmI GAANNNNTTC 1 cut(s) 1411
PfeI GAWTC 5 cut(s) 76, 242, 320, 842, 911
PkrI GCNGC 7 cut(s) 508, 804, 834, 1212, 1249, 1282, 1340
PleI GAGTC 4 cut(s) 473, 580, 610, 970
PpsI GAGTC 4 cut(s) 473, 580, 610, 970
PscI ACATGT 1 cut(s) 902
PsiI TTATAA 1 cut(s) 1157
Psp124BI GAGCTC 1 cut(s) 1266
PspN4I GGNNCC 1 cut(s) 897
PspPI GGNCC 1 cut(s) 757
PstI CTGCAG 1 cut(s) 834
PsuI RGATCY 2 cut(s) 405, 895
PvuII CAGCTG 1 cut(s) 727
RsaI GTAC 4 cut(s) 26, 360, 665, 1121
RsaNI GTAC 4 cut(s) 25, 359, 664, 1120
RseI CAYNNNNRTG 3 cut(s) 942, 993, 1299
SacI GAGCTC 1 cut(s) 1266
SapI GCTCTTC 1 cut(s) 684
SaqAI TTAA 6 cut(s) 285, 636, 750, 815, 887, 1268
SatI GCNGC 7 cut(s) 507, 803, 833, 1211, 1248, 1281, 1339
Sau3AI GATC 4 cut(s) 405, 517, 895, 1304
Sau96I GGNCC 1 cut(s) 757
ScaI AGTACT 1 cut(s) 665
SchI GAGTC 4 cut(s) 473, 581, 611, 971
ScrFI CCNGG 1 cut(s) 695
SduI GDGCHC 1 cut(s) 1266
SfaNI GCATC 8 cut(s) 60, 195, 370, 643, 946, 985, 1244, 1318
SfcI CTRYAG 2 cut(s) 830, 1192
SfuI TTCGAA 4 cut(s) 130, 145, 1393, 1405
SmiMI CAYNNNNRTG 3 cut(s) 942, 993, 1299
SmlI CTYRAG 3 cut(s) 44, 560, 886
SmoI CTYRAG 3 cut(s) 44, 560, 886
SsiI CCGC 2 cut(s) 476, 1210
SspI AATATT 3 cut(s) 631, 776, 1152
SspMI CTAG 3 cut(s) 237, 761, 822
SstI GAGCTC 1 cut(s) 1266
StyD4I CCNGG 1 cut(s) 693
TaaI ACNGT 3 cut(s) 290, 389, 1119
TaiI ACGT 1 cut(s) 1323
TaqI TCGA 7 cut(s) 130, 145, 609, 811, 1393, 1405, 1423
TaqII GACCGA 1 cut(s) 95
TatI WGTACW 2 cut(s) 24, 663
TauI GCSGC 1 cut(s) 1213
TfiI GAWTC 5 cut(s) 76, 242, 320, 842, 911
Tru1I TTAA 6 cut(s) 285, 636, 750, 815, 887, 1268
Tru9I TTAA 6 cut(s) 285, 636, 750, 815, 887, 1268
TscAI CASTG 4 cut(s) 215, 394, 663, 1296
TseI GCWGC 6 cut(s) 506, 802, 832, 1247, 1280, 1338
TspDTI ATGAA 8 cut(s) 17, 264, 302, 516, 633, 903, 1077, 1113
TspRI CASTG 4 cut(s) 215, 394, 663, 1296
Vha464I CTTAAG 1 cut(s) 886
XapI RAATTY 7 cut(s) 438, 452, 466, 490, 1396, 1407, 1417
XceI RCATGY 3 cut(s) 35, 677, 906
XmiI GTMKAC 1 cut(s) 1205
XmnI GAANNNNTTC 1 cut(s) 1411
XspI CTAG 3 cut(s) 237, 761, 822
ZrmI AGTACT 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.