Rw0G022010

Erwinia induced protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01021
OG0005096
Physical Location & Seq
Reverse (-)
3752 .. 4093
342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G022010.1

Sequence Viewer

Length: 342 bp
ATGGCTCTAGAGTGGATGGTGCTAGGCTACGCCATCGGCGTAGAGGCCATGATGGTCCTCCTCCTCACACTCATTGGCCTCGACTGCCCCCCCAAGGGCCTCATGGCCGTCACGCAAAACCTCCTCAAGCCCTTCCTCTCGGTGGTGCCCTTCTGCCTCTTCTTTCTCATGGACATCTACTGGAAGTATGAGAATCGGCCGAACTGCGACAACGACTCTTGCACGCCGTTCGAGCACCTCTGCCACCAGAAGTCCATCATGAAGAGCCAGTGCAACATCCTTCTCATAGACGGGCCACTGAGTCGAATCGGTTTGTGTGGTTTTCTGATCTTGAAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

113

Amino Acids

12.77

Weight (kDa)

6.03

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 145
AcoI YGGCCR 2 cut(s) 105, 197
AfiI CCNNNNNNNGG 3 cut(s) 94, 95, 142
AgsI TTSAA 1 cut(s) 334
Alw21I GWGCWC 1 cut(s) 237
AoxI GGCC 6 cut(s) 45, 76, 97, 105, 197, 293
AspS9I GGNCC 3 cut(s) 55, 97, 293
AvaII GGWCC 1 cut(s) 55
BaeGI GKGCMC 1 cut(s) 150
BanI GGYRCC 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 237
BccI CCATC 4 cut(s) 10, 41, 46, 263
BceAI ACGGC 2 cut(s) 92, 211
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BfaI CTAG 2 cut(s) 8, 23
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 3 cut(s) 55, 97, 293
BmiI GGNNCC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 93
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 95, 142
Bse1I ACTGG 2 cut(s) 185, 268
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 2 cut(s) 21, 276
BseLI CCNNNNNNNGG 3 cut(s) 94, 95, 142
BseMII CTCAG 1 cut(s) 290
BseNI ACTGG 2 cut(s) 185, 268
BseRI GAGGAG 3 cut(s) 50, 53, 113
BseSI GKGCMC 1 cut(s) 150
BseX3I CGGCCG 1 cut(s) 197
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 6 cut(s) 47, 78, 99, 107, 199, 295
BshNI GGYRCC 1 cut(s) 145
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 237
BslI CCNNNNNNNGG 3 cut(s) 94, 95, 142
BsnI GGCC 6 cut(s) 47, 78, 99, 107, 199, 295
Bsp1286I GDGCHC 2 cut(s) 150, 237
Bsp143I GATC 1 cut(s) 327
BspANI GGCC 6 cut(s) 47, 78, 99, 107, 199, 295
BspCNI CTCAG 1 cut(s) 291
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 147
BspQI GCTCTTC 1 cut(s) 257
BspT107I GGYRCC 1 cut(s) 145
BsrI ACTGG 2 cut(s) 185, 268
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 1 cut(s) 327
BssT1I CCWWGG 1 cut(s) 93
Bst6I CTCTTC 2 cut(s) 164, 257
BstC8I GCNNGC 1 cut(s) 224
BstDEI CTNAG 1 cut(s) 299
BstF5I GGATG 2 cut(s) 21, 276
BstKTI GATC 1 cut(s) 330
BstMBI GATC 1 cut(s) 327
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 2 cut(s) 84, 232
BstSLI GKGCMC 1 cut(s) 150
BstZI CGGCCG 1 cut(s) 197
BsuRI GGCC 6 cut(s) 47, 78, 99, 107, 199, 295
BtsCI GGATG 2 cut(s) 21, 276
BtsIMutI CAGTG 2 cut(s) 275, 296
Cac8I GCNNGC 1 cut(s) 224
CciI TCATGA 1 cut(s) 258
Cfr13I GGNCC 3 cut(s) 55, 97, 293
CviAII CATG 4 cut(s) 49, 103, 169, 259
DdeI CTNAG 1 cut(s) 299
DpnI GATC 1 cut(s) 329
DpnII GATC 1 cut(s) 327
EaeI YGGCCR 2 cut(s) 105, 197
EagI CGGCCG 1 cut(s) 197
Eam1104I CTCTTC 2 cut(s) 164, 257
EarI CTCTTC 2 cut(s) 164, 257
EclXI CGGCCG 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 55
Eco52I CGGCCG 1 cut(s) 197
EcoO109I RGGNCCY 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 4 cut(s) 52, 106, 172, 262
FaiI YATR 6 cut(s) 50, 104, 170, 189, 260, 287
FatI CATG 4 cut(s) 48, 102, 168, 258
FokI GGATG 2 cut(s) 28, 263
FspBI CTAG 2 cut(s) 8, 23
HaeIII GGCC 6 cut(s) 47, 78, 99, 107, 199, 295
Hin1II CATG 4 cut(s) 52, 106, 172, 262
HinfI GANTC 4 cut(s) 193, 215, 301, 306
Hpy188I TCNGA 1 cut(s) 327
Hpy188III TCNNGA 3 cut(s) 8, 259, 331
HpyAV CCTTC 3 cut(s) 142, 160, 290
HpyCH4V TGCA 2 cut(s) 222, 273
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 232
HpyF3I CTNAG 1 cut(s) 299
Hsp92II CATG 4 cut(s) 52, 106, 172, 262
Kzo9I GATC 1 cut(s) 327
LguI GCTCTTC 1 cut(s) 257
LpnPI CCDG 3 cut(s) 166, 260, 281
MaeI CTAG 2 cut(s) 8, 23
MaeIII GTNAC 1 cut(s) 109
MalI GATC 1 cut(s) 329
MboI GATC 1 cut(s) 327
MboII GAAGA 2 cut(s) 151, 274
MhlI GDGCHC 2 cut(s) 150, 237
MlyI GAGTC 2 cut(s) 209, 310
MwoI GCNNNNNNNGC 2 cut(s) 84, 232
NdeII GATC 1 cut(s) 327
NlaIII CATG 4 cut(s) 52, 106, 172, 262
NlaIV GGNNCC 1 cut(s) 147
NmuCI GTSAC 1 cut(s) 109
PagI TCATGA 1 cut(s) 258
PciSI GCTCTTC 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PfeI GAWTC 2 cut(s) 193, 306
PleI GAGTC 2 cut(s) 209, 309
PpsI GAGTC 2 cut(s) 209, 309
PspN4I GGNNCC 1 cut(s) 147
PspPI GGNCC 3 cut(s) 55, 97, 293
SapI GCTCTTC 1 cut(s) 257
Sau3AI GATC 1 cut(s) 327
Sau96I GGNCC 3 cut(s) 55, 97, 293
SchI GAGTC 2 cut(s) 209, 310
SduI GDGCHC 2 cut(s) 150, 237
SetI ASST 2 cut(s) 123, 240
SinI GGWCC 1 cut(s) 55
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SspMI CTAG 2 cut(s) 8, 23
StyI CCWWGG 1 cut(s) 93
TaqI TCGA 3 cut(s) 81, 231, 304
TfiI GAWTC 2 cut(s) 193, 306
TscAI CASTG 2 cut(s) 275, 303
TseFI GTSAC 1 cut(s) 109
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 275
TspRI CASTG 2 cut(s) 275, 303
VpaK11BI GGWCC 1 cut(s) 55
XbaI TCTAGA 1 cut(s) 7
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XspI CTAG 2 cut(s) 8, 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.