Rw1G026010

GRF1-interacting factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006105
Physical Location & Seq
Reverse (-)
52930468 .. 52933543
3076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G026010.1

Sequence Viewer

Length: 597 bp
ATGCAGCAGCAGCAGCAGCAGCCACAGCAAATGATGACCGGGATGCCTTCATTTCCACCCACTAACATCACCACCGAGCAAATTCAGAAGTGCCTTGATGAGAACAAAAAGTTGATTCTAGCAATATTGGACAATCAAAACCTTGGAAAACTTGCTGAGTGTGCCCAGTACCAAACTCAGCTTCAAAAGAATCTTATGTATTTAGCAGCAATTGCTGATGCTCAACCACAACCACAAGCACCAACAATGCCAGCCCAGATGGCCCCGCATCCTGCGATGCAACAAGCGGGATATTACATGCAGCATCCTCAGGCTGCAGCGGCAATGGCTCAGCAACAGGGTCTTTTCCCTCAAAAGATGCAAATGCAGTTCAATAGCCCACATCAAATGCACGAATCACACCAGCAGTTACACCAACAAGCCATGCAAGGCCAGATGGGAATGAGACCTGGAGGGGCCAATGGCATGCCTTCAATGCATCATACTGAGAGCACCCATGGAGGAAGCAAGCAAGACAACTCAGAGGCTGGGACAGGTGGCGATGGCCAGGGGAGCTCGGCTGGTGGGCACGGCAATGGTGACGGAGAAGATAAGTGA

Protein Analysis

198

Amino Acids

21.49

Weight (kDa)

6.01

Isoelectric Point (pI)

68.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSXT PF05030 22 - 79 7.4e-23 SSXT protein (N-terminal region)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 266, 287, 320
AcoI YGGCCR 1 cut(s) 544
AcsI RAATTY 1 cut(s) 81
AfaI GTAC 1 cut(s) 170
AgsI TTSAA 3 cut(s) 185, 373, 474
AjnI CCWGG 2 cut(s) 448, 546
AjuI GAANNNNNNNTTGG 2 cut(s) 165, 197
AluBI AGCT 2 cut(s) 181, 555
AluI AGCT 2 cut(s) 181, 555
Alw21I GWGCWC 2 cut(s) 494, 557
Alw26I GTCTC 1 cut(s) 439
AlwNI CAGNNNCTG 1 cut(s) 527
AoxI GGCC 4 cut(s) 261, 430, 456, 544
ApoI RAATTY 1 cut(s) 81
AspS9I GGNCC 2 cut(s) 262, 456
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 2 cut(s) 61, 590
AxyI CCTNAGG 1 cut(s) 309
BaeGI GKGCMC 2 cut(s) 166, 570
BalI TGGCCA 1 cut(s) 546
BanII GRGCYC 1 cut(s) 557
Bbv12I GWGCWC 2 cut(s) 494, 557
BccI CCATC 3 cut(s) 253, 430, 536
BceAI ACGGC 1 cut(s) 586
BciT130I CCWGG 2 cut(s) 450, 548
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 439
BfaI CTAG 1 cut(s) 119
BfmI CTRYAG 1 cut(s) 315
BglI GCCNNNNNGGC 1 cut(s) 260
BlpI GCTNAGC 1 cut(s) 330
Bme1390I CCNGG 3 cut(s) 40, 450, 548
BmgT120I GGNCC 2 cut(s) 262, 456
BmiI GGNNCC 2 cut(s) 264, 457
BmrFI CCNGG 3 cut(s) 40, 450, 548
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 7 cut(s) 33, 208, 267, 277, 313, 348, 487
BmuI ACTGGG 1 cut(s) 160
BpmI CTGGAG 1 cut(s) 471
Bpu1102I GCTNAGC 1 cut(s) 330
BpuMI CCSGG 1 cut(s) 40
BsaI GGTCTC 1 cut(s) 439
BsaJI CCNNGG 3 cut(s) 142, 496, 547
Bse1I ACTGG 1 cut(s) 166
Bse21I CCTNAGG 1 cut(s) 309
Bse3DI GCAATG 2 cut(s) 330, 580
BseBI CCWGG 2 cut(s) 450, 548
BseDI CCNNGG 3 cut(s) 142, 496, 547
BseGI GGATG 3 cut(s) 48, 268, 304
BseMI GCAATG 2 cut(s) 330, 580
BseMII CTCAG 6 cut(s) 147, 191, 323, 344, 477, 534
BseNI ACTGG 1 cut(s) 166
BseSI GKGCMC 2 cut(s) 166, 570
BseYI CCCAGC 1 cut(s) 527
BshFI GGCC 4 cut(s) 263, 432, 458, 546
BsiHKAI GWGCWC 2 cut(s) 494, 557
BsiSI CCGG 1 cut(s) 39
BslFI GGGAC 1 cut(s) 544
BsmAI GTCTC 1 cut(s) 439
BsmFI GGGAC 1 cut(s) 544
BsnI GGCC 4 cut(s) 263, 432, 458, 546
Bso31I GGTCTC 1 cut(s) 439
Bsp1286I GDGCHC 4 cut(s) 166, 494, 557, 570
Bsp1720I GCTNAGC 1 cut(s) 330
Bsp19I CCATGG 1 cut(s) 496
BspACI CCGC 3 cut(s) 266, 287, 320
BspANI GGCC 4 cut(s) 263, 432, 458, 546
BspCNI CTCAG 6 cut(s) 148, 190, 322, 343, 478, 533
BspLI GGNNCC 2 cut(s) 264, 457
BspMAI CTGCAG 1 cut(s) 319
BspTNI GGTCTC 1 cut(s) 439
BsrDI GCAATG 2 cut(s) 330, 580
BsrI ACTGG 1 cut(s) 166
BssECI CCNNGG 3 cut(s) 142, 496, 547
BssT1I CCWWGG 2 cut(s) 142, 496
Bst2UI CCWGG 2 cut(s) 450, 548
BstAPI GCANNNNNTGC 1 cut(s) 212
BstC8I GCNNGC 3 cut(s) 252, 467, 509
BstDEI CTNAG 6 cut(s) 156, 177, 309, 330, 486, 520
BstDSI CCRYGG 1 cut(s) 496
BstF5I GGATG 3 cut(s) 48, 268, 304
BstMAI GTCTC 1 cut(s) 439
BstNI CCWGG 2 cut(s) 450, 548
BstNSI RCATGY 2 cut(s) 301, 469
BstSCI CCNGG 3 cut(s) 38, 448, 546
BstSFI CTRYAG 1 cut(s) 315
BstSLI GKGCMC 2 cut(s) 166, 570
Bsu36I CCTNAGG 1 cut(s) 309
BsuRI GGCC 4 cut(s) 263, 432, 458, 546
BtgI CCRYGG 1 cut(s) 496
BtgZI GCGATG 2 cut(s) 290, 555
BtsCI GGATG 3 cut(s) 48, 268, 304
Cac8I GCNNGC 3 cut(s) 252, 467, 509
CaiI CAGNNNCTG 1 cut(s) 527
Cfr13I GGNCC 2 cut(s) 262, 456
Csp6I GTAC 1 cut(s) 169
CviAII CATG 4 cut(s) 298, 424, 466, 497
CviQI GTAC 1 cut(s) 169
DdeI CTNAG 6 cut(s) 156, 177, 309, 330, 486, 520
EaeI YGGCCR 1 cut(s) 544
Ecl136II GAGCTC 1 cut(s) 555
Eco130I CCWWGG 2 cut(s) 142, 496
Eco24I GRGCYC 1 cut(s) 557
Eco31I GGTCTC 1 cut(s) 439
Eco53kI GAGCTC 1 cut(s) 555
Eco81I CCTNAGG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 555
EcoRII CCWGG 2 cut(s) 448, 546
EcoT14I CCWWGG 2 cut(s) 142, 496
EcoT22I ATGCAT 1 cut(s) 480
EcoT38I GRGCYC 1 cut(s) 557
ErhI CCWWGG 2 cut(s) 142, 496
FaeI CATG 4 cut(s) 301, 427, 469, 500
FaiI YATR 6 cut(s) 197, 299, 425, 467, 483, 498
FaqI GGGAC 1 cut(s) 544
FatI CATG 4 cut(s) 297, 423, 465, 496
FauI CCCGC 2 cut(s) 273, 280
FokI GGATG 3 cut(s) 55, 255, 291
FriOI GRGCYC 1 cut(s) 557
FspBI CTAG 1 cut(s) 119
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 471
HaeIII GGCC 4 cut(s) 263, 432, 458, 546
HapII CCGG 1 cut(s) 39
Hin1II CATG 4 cut(s) 301, 427, 469, 500
HinfI GANTC 3 cut(s) 115, 190, 395
HpaII CCGG 1 cut(s) 39
HphI GGTGA 2 cut(s) 61, 590
Hpy188I TCNGA 2 cut(s) 87, 523
HpyAV CCTTC 2 cut(s) 57, 480
HpyCH4V TGCA 9 cut(s) 4, 280, 301, 317, 361, 367, 391, 427, 478
HpyF3I CTNAG 6 cut(s) 156, 177, 309, 330, 486, 520
Hsp92II CATG 4 cut(s) 301, 427, 469, 500
LmnI GCTCC 1 cut(s) 552
LweI GCATC 7 cut(s) 33, 208, 267, 277, 313, 348, 487
MaeI CTAG 1 cut(s) 119
MaeIII GTNAC 2 cut(s) 408, 578
MfeI CAATTG 1 cut(s) 210
MhlI GDGCHC 4 cut(s) 166, 494, 557, 570
MlsI TGGCCA 1 cut(s) 546
MluCI AATT 2 cut(s) 81, 210
MluNI TGGCCA 1 cut(s) 546
MnlI CCTC 5 cut(s) 318, 360, 446, 494, 517
Mox20I TGGCCA 1 cut(s) 546
Mph1103I ATGCAT 1 cut(s) 480
MscI TGGCCA 1 cut(s) 546
MslI CAYNNNNRTG 1 cut(s) 573
Msp20I TGGCCA 1 cut(s) 546
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 3 cut(s) 40, 450, 548
MunI CAATTG 1 cut(s) 210
MvaI CCWGG 2 cut(s) 450, 548
NciI CCSGG 1 cut(s) 40
NcoI CCATGG 1 cut(s) 496
NlaIII CATG 4 cut(s) 301, 427, 469, 500
NlaIV GGNNCC 2 cut(s) 264, 457
NmeAIII GCCGAG 1 cut(s) 536
NmuCI GTSAC 1 cut(s) 578
NsiI ATGCAT 1 cut(s) 480
NspI RCATGY 2 cut(s) 301, 469
PaeI GCATGC 1 cut(s) 469
PfeI GAWTC 3 cut(s) 115, 190, 395
Psp124BI GAGCTC 1 cut(s) 557
Psp6I CCWGG 2 cut(s) 448, 546
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 2 cut(s) 448, 546
PspN4I GGNNCC 2 cut(s) 264, 457
PspPI GGNCC 2 cut(s) 262, 456
PstI CTGCAG 1 cut(s) 319
PstNI CAGNNNCTG 1 cut(s) 527
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
RseI CAYNNNNRTG 1 cut(s) 573
SacI GAGCTC 1 cut(s) 557
Sau96I GGNCC 2 cut(s) 262, 456
ScrFI CCNGG 3 cut(s) 40, 450, 548
SduI GDGCHC 4 cut(s) 166, 494, 557, 570
SetI ASST 5 cut(s) 144, 183, 451, 538, 557
SfaNI GCATC 7 cut(s) 33, 208, 267, 277, 313, 348, 487
SfcI CTRYAG 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 573
SphI GCATGC 1 cut(s) 469
Sse9I AATT 2 cut(s) 81, 210
SsiI CCGC 3 cut(s) 266, 287, 320
SspI AATATT 1 cut(s) 126
SspMI CTAG 1 cut(s) 119
SstI GAGCTC 1 cut(s) 557
StyD4I CCNGG 3 cut(s) 38, 448, 546
StyI CCWWGG 2 cut(s) 142, 496
TasI AATT 2 cut(s) 81, 210
TauI GCSGC 1 cut(s) 323
TfiI GAWTC 3 cut(s) 115, 190, 395
TseFI GTSAC 1 cut(s) 578
Tsp45I GTSAC 1 cut(s) 578
TspDTI ATGAA 1 cut(s) 39
TspGWI ACGGA 1 cut(s) 597
XapI RAATTY 1 cut(s) 81
XceI RCATGY 2 cut(s) 301, 469
XspI CTAG 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.