Rw2G053450

Zinc finger protein CONSTANS-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009406
Physical Location & Seq
Forward (+)
81197739 .. 81199224
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G053450.1

Sequence Viewer

Length: 1074 bp
ATGTGCCACCACAACAAGAACAGTCCACCTCCGAATCATCATCACGTGAAATTTCAAACCAGGTCGATTTCTATGATGAAGATGATCAAGGAGGAGGAAGAAGAAGAAATCAAGCCGCTAAAGAGAGCTTCACTGAAACCGAAGCGATCGGCGGCGGCGGCGCGGAAATCGAGGAGCCGGAGAAGAAAGCCGAAGTACTTGAGCCTCCGCTCGCCGCGGCTCTCCGCCCGAAACAACGTCGTCGCCGCCTCCTCCGCCGATACGGATTCCAAGATGACTGCTGCTACTACTACTACTACGGCGGTGACGCGCCGCGGCCACCAGCTTAATTTGTTTCCGCTGCATCCGGAGCTGAACGCGGCGGAGGCGGATCACCAGTCCGACGACAACGTGGCTCTGCTGTTCGAATCCGACGGCGGAGCCACTCTCCACGGGCTGCTCACCTCCGCCGACACGGCCACCACGACGACGACGTCGACAATGTCGTCCGATCACGACCAGGCCGAGTCCAGTTACGGCTACCGCCGCGGAATGGAACGCGGCGACGATATCGTACGGTCGGCGATGAGGGGGAGCGGAGGAGATCGGAGCGTCGAGAAGTGGGTGTGTTACTCGGAGCTGGTGAAGGAGGAGGTCGAGTTAACGGCGACGACGACCACCAGCTCGGCGAGCACGTGTTGCAGCGTCGGCGATGATCGGAAAGGTCATCGGGATCGACGGCTGGCGTTGAAGCTTGACTATGAGGAGGTCTTGAATGCTTGGTCCGATAAGGGGTCGCTGTACATTAAAACCGAAGACGGCGAGCCGCCGCCGCAGACGGTGCCGGACTTGCTTCAGGATCAGCTCTTTCACCACCTCCATGATTCTACTGCCAATGATTTATGGAGGGTGCCTGAAGAGAGTGGTAGCGGTAGCGGTAGCTTGGAGAGGAAGATTCAAAGACAGGCGAGTTTATTGAGATACAAGCGGAAAAGGGAGAACAGACTGTTTTCTAAGCGGATTCGGTATGAAGTTCGGAAGCTAAATGCCGAGAAACGACCCCGTATGAAGGGTCGGTTTGTGAAGAGTAGTTGA

Protein Analysis

357

Amino Acids

40.22

Weight (kDa)

9.6

Isoelectric Point (pI)

55.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCT PF06203 314 - 356 3.6e-17 CCT motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 472, 484
AatII GACGTC 1 cut(s) 476
AccB1I GGYRCC 2 cut(s) 820, 889
AccBSI CCGCTC 2 cut(s) 210, 576
AccI GTMKAC 1 cut(s) 476
AccII CGCG 7 cut(s) 163, 217, 310, 315, 359, 528, 540
AccIII TCCGGA 1 cut(s) 346
AclWI GGATC 3 cut(s) 378, 720, 846
AcoI YGGCCR 2 cut(s) 316, 456
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 2 cut(s) 818, 915
AcvI CACGTG 2 cut(s) 46, 675
AcyI GRCGYC 1 cut(s) 473
AfaI GTAC 3 cut(s) 197, 555, 782
AfiI CCNNNNNNNGG 3 cut(s) 532, 771, 1048
AflIII ACRYGT 1 cut(s) 674
AgsI TTSAA 4 cut(s) 56, 730, 754, 938
AjnI CCWGG 2 cut(s) 59, 498
AluBI AGCT 9 cut(s) 128, 325, 352, 619, 663, 733, 844, 921, 1021
AluI AGCT 9 cut(s) 128, 325, 352, 619, 663, 733, 844, 921, 1021
Alw21I GWGCWC 1 cut(s) 674
AlwI GGATC 3 cut(s) 378, 720, 846
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 3 cut(s) 316, 456, 501
ApeKI GCWGC 4 cut(s) 281, 340, 436, 681
ApoI RAATTY 1 cut(s) 50
AspLEI GCGC 2 cut(s) 163, 312
AspS9I GGNCC 1 cut(s) 762
AsuHPI GGTGA 5 cut(s) 316, 365, 433, 634, 842
AsuII TTCGAA 1 cut(s) 405
AvaII GGWCC 1 cut(s) 762
BanI GGYRCC 2 cut(s) 820, 889
BbrPI CACGTG 2 cut(s) 46, 675
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 1 cut(s) 674
BbvI GCAGC 4 cut(s) 268, 327, 423, 693
BceAI ACGGC 7 cut(s) 315, 430, 471, 532, 660, 734, 814
BciT130I CCWGG 2 cut(s) 61, 500
BclI TGATCA 1 cut(s) 84
BglI GCCNNNNNGGC 1 cut(s) 455
BmcAI AGTACT 1 cut(s) 197
Bme1390I CCNGG 2 cut(s) 61, 500
Bme18I GGWCC 1 cut(s) 762
BmgT120I GGNCC 1 cut(s) 762
BmiI GGNNCC 4 cut(s) 176, 421, 822, 891
BmrFI CCNGG 2 cut(s) 61, 500
BmsI GCATC 1 cut(s) 352
BpiI GAAGAC 1 cut(s) 801
BplI GAGNNNNNCTC 2 cut(s) 411, 443
Bpu14I TTCGAA 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 220
BsaAI YACGTR 2 cut(s) 46, 675
BsaHI GRCGYC 1 cut(s) 473
BsaJI CCNNGG 4 cut(s) 215, 313, 430, 526
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 3 cut(s) 532, 771, 1048
Bse1I ACTGG 2 cut(s) 376, 510
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 2 cut(s) 61, 500
BseDI CCNNGG 4 cut(s) 215, 313, 430, 526
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 3 cut(s) 532, 771, 1048
BseNI ACTGG 2 cut(s) 376, 510
BseRI GAGGAG 6 cut(s) 107, 187, 241, 594, 644, 758
BseXI GCAGC 4 cut(s) 268, 327, 423, 693
Bsh1236I CGCG 7 cut(s) 163, 217, 310, 315, 359, 528, 540
Bsh1285I CGRYCG 2 cut(s) 149, 560
BshFI GGCC 3 cut(s) 318, 458, 503
BshNI GGYRCC 2 cut(s) 820, 889
BsiEI CGRYCG 2 cut(s) 149, 560
BsiHKAI GWGCWC 1 cut(s) 674
BsiSI CCGG 3 cut(s) 178, 347, 824
BsiWI CGTACG 1 cut(s) 553
BslI CCNNNNNNNGG 3 cut(s) 532, 771, 1048
BsmI GAATGC 1 cut(s) 760
BsnI GGCC 3 cut(s) 318, 458, 503
Bsp119I TTCGAA 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 674
Bsp13I TCCGGA 1 cut(s) 346
Bsp1407I TGTACA 1 cut(s) 780
Bsp143I GATC 8 cut(s) 84, 146, 370, 490, 583, 694, 712, 838
BspANI GGCC 3 cut(s) 318, 458, 503
BspEI TCCGGA 1 cut(s) 346
BspFNI CGCG 7 cut(s) 163, 217, 310, 315, 359, 528, 540
BspLI GGNNCC 4 cut(s) 176, 421, 822, 891
BspPI GGATC 3 cut(s) 378, 720, 846
BspT104I TTCGAA 1 cut(s) 405
BspT107I GGYRCC 2 cut(s) 820, 889
BsrBI CCGCTC 2 cut(s) 210, 576
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 2 cut(s) 376, 510
BssECI CCNNGG 4 cut(s) 215, 313, 430, 526
BssMI GATC 8 cut(s) 84, 146, 370, 490, 583, 694, 712, 838
BssNI GRCGYC 1 cut(s) 473
Bst2UI CCWGG 2 cut(s) 61, 500
Bst4CI ACNGT 4 cut(s) 23, 558, 820, 987
Bst6I CTCTTC 2 cut(s) 891, 1058
BstACI GRCGYC 1 cut(s) 473
BstAPI GCANNNNNTGC 2 cut(s) 678, 820
BstAUI TGTACA 1 cut(s) 780
BstBAI YACGTR 2 cut(s) 46, 675
BstBI TTCGAA 1 cut(s) 405
BstC8I GCNNGC 4 cut(s) 212, 670, 723, 803
BstDEI CTNAG 1 cut(s) 993
BstDSI CCRYGG 4 cut(s) 215, 313, 430, 526
BstF5I GGATG 1 cut(s) 343
BstFNI CGCG 7 cut(s) 163, 217, 310, 315, 359, 528, 540
BstHHI GCGC 2 cut(s) 163, 312
BstKTI GATC 8 cut(s) 87, 149, 373, 493, 586, 697, 715, 841
BstMBI GATC 8 cut(s) 84, 146, 370, 490, 583, 694, 712, 838
BstMCI CGRYCG 2 cut(s) 149, 560
BstNI CCWGG 2 cut(s) 61, 500
BstSCI CCNGG 2 cut(s) 59, 498
BstUI CGCG 7 cut(s) 163, 217, 310, 315, 359, 528, 540
BstV1I GCAGC 4 cut(s) 268, 327, 423, 693
BstV2I GAAGAC 1 cut(s) 801
BsuRI GGCC 3 cut(s) 318, 458, 503
BtgI CCRYGG 4 cut(s) 215, 313, 430, 526
BtgZI GCGATG 2 cut(s) 578, 705
BtsCI GGATG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 131
Cac8I GCNNGC 4 cut(s) 212, 670, 723, 803
CfoI GCGC 2 cut(s) 163, 312
Cfr13I GGNCC 1 cut(s) 762
Cfr42I CCGCGG 3 cut(s) 218, 316, 529
CseI GACGC 3 cut(s) 316, 580, 673
CsiI ACCWGGT 1 cut(s) 59
Csp6I GTAC 3 cut(s) 196, 554, 781
CviAII CATG 1 cut(s) 860
CviQI GTAC 3 cut(s) 196, 554, 781
DdeI CTNAG 1 cut(s) 993
DpnI GATC 8 cut(s) 86, 148, 372, 492, 585, 696, 714, 840
DpnII GATC 8 cut(s) 84, 146, 370, 490, 583, 694, 712, 838
DrdI GACNNNNNNGTC 2 cut(s) 472, 484
DseDI GACNNNNNNGTC 2 cut(s) 472, 484
EaeI YGGCCR 2 cut(s) 316, 456
Eam1104I CTCTTC 2 cut(s) 891, 1058
EarI CTCTTC 2 cut(s) 891, 1058
EciI GGCGGA 6 cut(s) 214, 244, 377, 383, 432, 436
Eco32I GATATC 1 cut(s) 550
Eco47I GGWCC 1 cut(s) 762
Eco57I CTGAAG 2 cut(s) 818, 915
Eco72I CACGTG 2 cut(s) 46, 675
EcoRII CCWGG 2 cut(s) 59, 498
EcoRV GATATC 1 cut(s) 550
FaeI CATG 1 cut(s) 863
FaiI YATR 6 cut(s) 74, 741, 861, 883, 1008, 1046
FatI CATG 1 cut(s) 859
FbaI TGATCA 1 cut(s) 84
FblI GTMKAC 1 cut(s) 476
FokI GGATG 1 cut(s) 330
GlaI GCGC 2 cut(s) 162, 311
HaeIII GGCC 3 cut(s) 318, 458, 503
HapII CCGG 3 cut(s) 178, 347, 824
HgaI GACGC 3 cut(s) 316, 580, 673
HhaI GCGC 2 cut(s) 163, 312
Hin1I GRCGYC 1 cut(s) 473
Hin1II CATG 1 cut(s) 863
Hin6I GCGC 2 cut(s) 161, 310
HinP1I GCGC 2 cut(s) 161, 310
HincII GTYRAC 2 cut(s) 477, 642
HindII GTYRAC 2 cut(s) 477, 642
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 7 cut(s) 34, 266, 407, 506, 863, 934, 1000
HpaI GTTAAC 1 cut(s) 642
HpaII CCGG 3 cut(s) 178, 347, 824
HphI GGTGA 5 cut(s) 316, 365, 433, 634, 842
Hpy166II GTNNAC 3 cut(s) 26, 477, 642
Hpy188I TCNGA 9 cut(s) 33, 382, 412, 490, 588, 616, 699, 766, 1017
Hpy188III TCNNGA 6 cut(s) 347, 494, 595, 710, 751, 836
Hpy8I GTNNAC 3 cut(s) 26, 477, 642
HpyAV CCTTC 2 cut(s) 619, 1042
HpyCH4III ACNGT 4 cut(s) 23, 558, 820, 987
HpyCH4IV ACGT 5 cut(s) 45, 237, 390, 473, 674
HpyCH4V TGCA 2 cut(s) 343, 681
HpyF3I CTNAG 1 cut(s) 993
HpySE526I ACGT 5 cut(s) 45, 237, 390, 473, 674
Hsp92I GRCGYC 1 cut(s) 473
Hsp92II CATG 1 cut(s) 863
HspAI GCGC 2 cut(s) 161, 310
Kpn2I TCCGGA 1 cut(s) 346
Ksp22I TGATCA 1 cut(s) 84
KspAI GTTAAC 1 cut(s) 642
KspI CCGCGG 3 cut(s) 218, 316, 529
Kzo9I GATC 8 cut(s) 84, 146, 370, 490, 583, 694, 712, 838
LmnI GCTCC 6 cut(s) 174, 349, 419, 573, 588, 616
Lsp1109I GCAGC 4 cut(s) 268, 327, 423, 693
LweI GCATC 1 cut(s) 352
MabI ACCWGGT 1 cut(s) 59
MaeII ACGT 5 cut(s) 45, 237, 390, 473, 674
MaeIII GTNAC 3 cut(s) 304, 512, 608
MalI GATC 8 cut(s) 86, 148, 372, 492, 585, 696, 714, 840
MbiI CCGCTC 2 cut(s) 210, 576
MboI GATC 8 cut(s) 84, 146, 370, 490, 583, 694, 712, 838
MboII GAAGA 8 cut(s) 91, 110, 113, 116, 195, 806, 908, 943
MhlI GDGCHC 1 cut(s) 674
MluCI AATT 2 cut(s) 50, 328
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 2 cut(s) 405, 435
MroI TCCGGA 1 cut(s) 346
MseI TTAA 3 cut(s) 327, 641, 786
MslI CAYNNNNRTG 1 cut(s) 858
MspA1I CMGCKG 4 cut(s) 217, 315, 340, 528
MspI CCGG 3 cut(s) 178, 347, 824
MspR9I CCNGG 2 cut(s) 61, 500
Mva1269I GAATGC 1 cut(s) 760
MvaI CCWGG 2 cut(s) 61, 500
MvnI CGCG 7 cut(s) 163, 217, 310, 315, 359, 528, 540
NdeII GATC 8 cut(s) 84, 146, 370, 490, 583, 694, 712, 838
NlaIII CATG 1 cut(s) 863
NlaIV GGNNCC 4 cut(s) 176, 421, 822, 891
NmeAIII GCCGAG 3 cut(s) 529, 644, 1054
NmuCI GTSAC 1 cut(s) 304
NspV TTCGAA 1 cut(s) 405
PcsI WCGNNNNNNNCGW 7 cut(s) 461, 470, 473, 482, 501, 650, 671
PctI GAATGC 1 cut(s) 760
PfeI GAWTC 6 cut(s) 34, 266, 407, 863, 934, 1000
Pfl23II CGTACG 1 cut(s) 553
PflFI GACNNNGTC 2 cut(s) 472, 481
Ple19I CGATCG 1 cut(s) 149
PleI GAGTC 1 cut(s) 514
PmaCI CACGTG 2 cut(s) 46, 675
PmlI CACGTG 2 cut(s) 46, 675
PpsI GAGTC 1 cut(s) 514
Ppu21I YACGTR 2 cut(s) 46, 675
Psp6I CCWGG 2 cut(s) 59, 498
PspCI CACGTG 2 cut(s) 46, 675
PspGI CCWGG 2 cut(s) 59, 498
PspLI CGTACG 1 cut(s) 553
PspN4I GGNNCC 4 cut(s) 176, 421, 822, 891
PspPI GGNCC 1 cut(s) 762
PsyI GACNNNGTC 2 cut(s) 472, 481
PvuI CGATCG 1 cut(s) 149
RsaI GTAC 3 cut(s) 197, 555, 782
RsaNI GTAC 3 cut(s) 196, 554, 781
RseI CAYNNNNRTG 1 cut(s) 858
SacII CCGCGG 3 cut(s) 218, 316, 529
SalI GTCGAC 1 cut(s) 475
SaqAI TTAA 3 cut(s) 327, 641, 786
Sau3AI GATC 8 cut(s) 84, 146, 370, 490, 583, 694, 712, 838
Sau96I GGNCC 1 cut(s) 762
ScaI AGTACT 1 cut(s) 197
SchI GAGTC 1 cut(s) 515
ScrFI CCNGG 2 cut(s) 61, 500
SduI GDGCHC 1 cut(s) 674
SexAI ACCWGGT 1 cut(s) 59
SfaNI GCATC 1 cut(s) 352
Sfr303I CCGCGG 3 cut(s) 218, 316, 529
SfuI TTCGAA 1 cut(s) 405
SgrBI CCGCGG 3 cut(s) 218, 316, 529
SinI GGWCC 1 cut(s) 762
SmiMI CAYNNNNRTG 1 cut(s) 858
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 2 cut(s) 50, 328
StyD4I CCNGG 2 cut(s) 59, 498
TaaI ACNGT 4 cut(s) 23, 558, 820, 987
TaiI ACGT 5 cut(s) 48, 240, 393, 476, 677
TaqI TCGA 7 cut(s) 65, 170, 405, 476, 594, 636, 715
TasI AATT 2 cut(s) 50, 328
TatI WGTACW 2 cut(s) 195, 780
TfiI GAWTC 6 cut(s) 34, 266, 407, 863, 934, 1000
Tru1I TTAA 3 cut(s) 327, 641, 786
Tru9I TTAA 3 cut(s) 327, 641, 786
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 4 cut(s) 281, 340, 436, 681
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 3 cut(s) 92, 1023, 1061
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 1 cut(s) 138
Tth111I GACNNNGTC 2 cut(s) 472, 481
VpaK11BI GGWCC 1 cut(s) 762
XapI RAATTY 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 476
ZraI GACGTC 1 cut(s) 474
ZrmI AGTACT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.