AT1G05040

nuclear envelope reassembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004014
Physical Location & Seq
Reverse (-)
1447369 .. 1448463
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05040.2

Sequence Viewer

Length: 501 bp
ATGAAGGAAGAAGATACTAACGTCACTAGGTTCTGCAAGGCAACGTCTGCGTGTAAAGATGCAGCATTCTACTACCTTGAAGGATTCGACTGGAATCTCGAAGACGCCATCTCTGGTTTCCTCGGTGATCAACTTCCTCCGCTCAAAATAAGAGCGACTCCACGGAGGGTGAATGAATGGCGAGAGCGATCTCGATCTCCTTTAAGAAGAAGATACTCTACTACCTCTGTCTCGGAATTTACGCTGAAGATGCGCCAAGATGAGATAACCATAACGAAAAGCTCTGATACGGGTGCTTTGGCCACCAGTTTGGATGATAGTGGAAGAGAGCTTCATGATTCTGATGATATATCTCATGGAGAAAAAATTGTGTCTATAGCTAATCCGGTGAATCAAGAGTTTAAAGAAGAGGGATCATCAGTTCAAGCTACGAAGATTTGTGAATCCACCTCCATTGAGATTCACTTGCCTAGACCGGATCCTTATTCGGACTCTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
SEP UBA_4 UBX

Protein Analysis

166

Amino Acids

18.75

Weight (kDa)

4.98

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_4 PF14555 6 - 44 8.7e-07 UBA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 142
AciI CCGC 1 cut(s) 140
AclWI GGATC 3 cut(s) 421, 473, 486
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 1 cut(s) 236
AcuI CTGAAG 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 105
AgsI TTSAA 2 cut(s) 80, 425
AloI GAACNNNNNNTCC 2 cut(s) 405, 437
AluBI AGCT 4 cut(s) 282, 331, 380, 428
AluI AGCT 4 cut(s) 282, 331, 380, 428
Alw26I GTCTC 1 cut(s) 235
AlwI GGATC 3 cut(s) 421, 473, 486
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 1 cut(s) 62
ApoI RAATTY 1 cut(s) 236
AspLEI GCGC 1 cut(s) 255
AsuHPI GGTGA 3 cut(s) 137, 181, 400
BaeI ACNNNNGTAYC 1 cut(s) 39
BalI TGGCCA 1 cut(s) 302
BamHI GGATCC 1 cut(s) 478
BarI GAAGNNNNNNTAC 2 cut(s) 202, 234
BbsI GAAGAC 1 cut(s) 108
BbvI GCAGC 1 cut(s) 74
BccI CCATC 1 cut(s) 116
BclI TGATCA 1 cut(s) 127
BcoDI GTCTC 1 cut(s) 235
BfaI CTAG 2 cut(s) 27, 471
BfmI CTRYAG 1 cut(s) 375
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
BmiI GGNNCC 1 cut(s) 480
BmsI GCATC 2 cut(s) 49, 240
BpiI GAAGAC 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 2 cut(s) 121, 161
BsaWI WCCGGW 2 cut(s) 385, 475
Bse1I ACTGG 2 cut(s) 95, 306
Bse8I GATNNNNATC 1 cut(s) 193
BseDI CCNNGG 2 cut(s) 121, 161
BseGI GGATG 1 cut(s) 319
BseJI GATNNNNATC 1 cut(s) 193
BseNI ACTGG 2 cut(s) 95, 306
BseXI GCAGC 1 cut(s) 74
BshFI GGCC 1 cut(s) 302
BsiSI CCGG 2 cut(s) 386, 476
BsmAI GTCTC 1 cut(s) 235
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 1 cut(s) 302
Bsp143I GATC 5 cut(s) 127, 188, 194, 413, 478
BspACI CCGC 1 cut(s) 140
BspANI GGCC 1 cut(s) 302
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 1 cut(s) 480
BspPI GGATC 3 cut(s) 421, 473, 486
BsrBI CCGCTC 1 cut(s) 142
BsrI ACTGG 2 cut(s) 95, 306
BssECI CCNNGG 2 cut(s) 121, 161
BssMI GATC 5 cut(s) 127, 188, 194, 413, 478
BssNI GRCGYC 1 cut(s) 105
Bst6I CTCTTC 2 cut(s) 319, 402
BstACI GRCGYC 1 cut(s) 105
BstAPI GCANNNNNTGC 1 cut(s) 47
BstDSI CCRYGG 1 cut(s) 161
BstF5I GGATG 1 cut(s) 319
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 5 cut(s) 130, 191, 197, 416, 481
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 5 cut(s) 127, 188, 194, 413, 478
BstMWI GCNNNNNNNGC 2 cut(s) 47, 250
BstSFI CTRYAG 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 74
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 478
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 1 cut(s) 302
BtgI CCRYGG 1 cut(s) 161
BtsCI GGATG 1 cut(s) 319
CciI TCATGA 1 cut(s) 334
CfoI GCGC 1 cut(s) 255
CseI GACGC 1 cut(s) 113
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 2 cut(s) 335, 356
CviJI RGCY 5 cut(s) 282, 302, 331, 380, 428
CviKI_1 RGCY 5 cut(s) 282, 302, 331, 380, 428
DpnI GATC 5 cut(s) 129, 190, 196, 415, 480
DpnII GATC 5 cut(s) 127, 188, 194, 413, 478
DraI TTTAAA 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 2 cut(s) 319, 402
EarI CTCTTC 2 cut(s) 319, 402
Eco57I CTGAAG 1 cut(s) 266
FaeI CATG 2 cut(s) 338, 359
FaiI YATR 5 cut(s) 272, 336, 350, 357, 377
FatI CATG 2 cut(s) 334, 355
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 1 cut(s) 63
FokI GGATG 1 cut(s) 326
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 2 cut(s) 27, 471
GlaI GCGC 1 cut(s) 254
GluI GCNGC 1 cut(s) 63
HaeIII GGCC 1 cut(s) 302
HapII CCGG 2 cut(s) 386, 476
HgaI GACGC 1 cut(s) 113
HhaI GCGC 1 cut(s) 255
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 2 cut(s) 338, 359
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HinfI GANTC 8 cut(s) 84, 94, 157, 338, 391, 443, 460, 491
HpaII CCGG 2 cut(s) 386, 476
HphI GGTGA 3 cut(s) 137, 181, 400
Hpy188I TCNGA 5 cut(s) 235, 286, 343, 490, 496
Hpy188III TCNNGA 4 cut(s) 98, 192, 335, 395
HpyAV CCTTC 1 cut(s) 74
HpyCH4IV ACGT 2 cut(s) 21, 44
HpyCH4V TGCA 2 cut(s) 36, 62
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 250
HpySE526I ACGT 2 cut(s) 21, 44
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 2 cut(s) 338, 359
HspAI GCGC 1 cut(s) 253
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 5 cut(s) 127, 188, 194, 413, 478
LpnPI CCDG 5 cut(s) 76, 99, 319, 399, 489
Lsp1109I GCAGC 1 cut(s) 74
LweI GCATC 2 cut(s) 49, 240
MaeI CTAG 2 cut(s) 27, 471
MaeII ACGT 2 cut(s) 21, 44
MaeIII GTNAC 1 cut(s) 22
MalI GATC 5 cut(s) 129, 190, 196, 415, 480
MbiI CCGCTC 1 cut(s) 142
MboI GATC 5 cut(s) 127, 188, 194, 413, 478
MboII GAAGA 9 cut(s) 20, 23, 113, 219, 222, 259, 336, 419, 445
MflI RGATCY 1 cut(s) 478
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 2 cut(s) 236, 366
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 2 cut(s) 151, 485
MnlI CCTC 6 cut(s) 131, 147, 159, 235, 403, 460
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MseI TTAA 2 cut(s) 203, 402
Msp20I TGGCCA 1 cut(s) 302
MspI CCGG 2 cut(s) 386, 476
Mva1269I GAATGC 1 cut(s) 65
MwoI GCNNNNNNNGC 2 cut(s) 47, 250
NdeII GATC 5 cut(s) 127, 188, 194, 413, 478
NlaIII CATG 2 cut(s) 338, 359
NlaIV GGNNCC 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 22
PagI TCATGA 1 cut(s) 334
PctI GAATGC 1 cut(s) 65
PfeI GAWTC 6 cut(s) 84, 94, 338, 391, 443, 460
PkrI GCNGC 1 cut(s) 64
PleI GAGTC 2 cut(s) 151, 485
PpsI GAGTC 2 cut(s) 151, 485
PspN4I GGNNCC 1 cut(s) 480
PsuI RGATCY 1 cut(s) 478
SaqAI TTAA 2 cut(s) 203, 402
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 5 cut(s) 127, 188, 194, 413, 478
SchI GAGTC 2 cut(s) 151, 485
SfaNI GCATC 2 cut(s) 49, 240
SfcI CTRYAG 1 cut(s) 375
Sse9I AATT 2 cut(s) 236, 366
SsiI CCGC 1 cut(s) 140
SspMI CTAG 2 cut(s) 27, 471
TaiI ACGT 2 cut(s) 24, 47
TaqI TCGA 3 cut(s) 87, 99, 193
TasI AATT 2 cut(s) 236, 366
TfiI GAWTC 6 cut(s) 84, 94, 338, 391, 443, 460
Tru1I TTAA 2 cut(s) 203, 402
Tru9I TTAA 2 cut(s) 203, 402
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 3 cut(s) 17, 189, 323
TspGWI ACGGA 1 cut(s) 178
XapI RAATTY 1 cut(s) 236
XspI CTAG 2 cut(s) 27, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.