AT1G25682

Family of unknown function (DUF572)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006850
Physical Location & Seq
Reverse (-)
9002285 .. 9004762
2478 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G25682.2

Sequence Viewer

Length: 933 bp
ATGTCGACTCTATCAGCTGCCAGGGCAGATAACTTTTACTATCCTCCAGAATGGACTCCGGATCAAGGTTCCTTGAACAAGTTTCAAGGACAGCATCCTCTCAGAGAGAGGGCAAAGAAAATTGGCGAGGGAATTTTGGTCATAAGGTTCGAGATGCCCTATAATATATGGTGTGGTGGCTGTAGTTCTATGATCGCCAAGGGTGTTCGATTTAATGCAGAAAAGAAGCAAGTTGGCAACTATTATTCTACAAAGATATGGAGCTTTGCAATGAAATCACCATGCTGTAAACATGAGATAGTCATTCAGACAGATCCTCAGAACTGCGAGTATGTAATCACTAGTGGTGCCCAGAAGAAGGTTGAGGAATATGAAGCAGAAGATGCAGAAACCATGGAGCTCACTGCTGAGCAAGAGAAGGGAAAGCTTGCAGACCCCTTTTATCGTCTAGAGCACCAGGAAGTAGATTTACAGAAGAAGAAAGCAGCTGAACCGCTTTTGGTCCGTCTTCAGCGAGTCTCAGATGCAAGACATGCTGATGATTACTCCCTAAACAAAGCTCTACGTGCACAACTTAGGCGACACAGAAAACGTGTAGCAGAGGAAGAGACTGCTTCAAGGAAGCTAGGCTTGGGGATAAGACTGCTTCCAAAGAGTGAAGAAGATATTAAAGCTGCCTCAAACGTGAAATTCAAGAGCAAGTTTGATAAGAATCGAAAGGACAAACGAGCACTGATCCATGCATCTTCCATTTTTCCCGAGTCGTCTTACTCATCGAGCAAGAAAAGAATGGAGCTGGAAGCAAAAAGAAGGAAAATAAGCGCAGCATCCGCATCAAGCCTATTGAGAGGAGGATTCAAAGCTTCATCTTTATCTACAAACCCATCAGCAAGCAAGCCCAAGGTTTCATCAGTTTCTGTAAGGAAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

310

Amino Acids

35.05

Weight (kDa)

9.69

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 13 - 279 4.6e-63 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 347
AccI GTMKAC 1 cut(s) 5
AccIII TCCGGA 1 cut(s) 58
AciI CCGC 2 cut(s) 494, 831
AclWI GGATC 3 cut(s) 69, 308, 730
AcsI RAATTY 2 cut(s) 132, 689
AcuI CTGAAG 1 cut(s) 494
AfiI CCNNNNNNNGG 2 cut(s) 65, 358
AflIII ACRYGT 1 cut(s) 592
AgsI TTSAA 5 cut(s) 76, 86, 618, 694, 859
AhlI ACTAGT 1 cut(s) 341
AjnI CCWGG 2 cut(s) 20, 456
AjuI GAANNNNNNNTTGG 2 cut(s) 614, 646
Alw21I GWGCWC 4 cut(s) 402, 456, 571, 733
Alw26I GTCTC 2 cut(s) 523, 602
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 3 cut(s) 69, 308, 730
AlwNI CAGNNNCTG 1 cut(s) 917
Ama87I CYCGRG 1 cut(s) 758
Aor13HI TCCGGA 1 cut(s) 58
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 4 cut(s) 17, 485, 674, 824
ApoI RAATTY 2 cut(s) 132, 689
AspLEI GCGC 1 cut(s) 824
AspS9I GGNCC 1 cut(s) 502
AsuHPI GGTGA 1 cut(s) 270
AvaI CYCGRG 1 cut(s) 758
AvaII GGWCC 1 cut(s) 502
BaeGI GKGCMC 2 cut(s) 352, 571
BanI GGYRCC 1 cut(s) 347
BanII GRGCYC 1 cut(s) 402
BarI GAAGNNNNNNTAC 2 cut(s) 453, 485
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 4 cut(s) 402, 456, 571, 733
BbvI GCAGC 4 cut(s) 4, 497, 661, 836
BccI CCATC 1 cut(s) 892
BciT130I CCWGG 2 cut(s) 22, 458
BcoDI GTCTC 2 cut(s) 523, 602
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 4 cut(s) 342, 449, 626, 931
BfmI CTRYAG 1 cut(s) 181
BisI GCNGC 4 cut(s) 18, 486, 675, 825
BlpI GCTNAGC 1 cut(s) 408
BlsI GCNGC 4 cut(s) 19, 487, 676, 826
Bme1390I CCNGG 2 cut(s) 22, 458
Bme18I GGWCC 1 cut(s) 502
BmeT110I CYCGRG 1 cut(s) 758
BmgT120I GGNCC 1 cut(s) 502
BmiI GGNNCC 2 cut(s) 70, 349
BmrFI CCNGG 2 cut(s) 22, 458
BmsI GCATC 7 cut(s) 103, 144, 373, 514, 752, 836, 842
BpiI GAAGAC 1 cut(s) 500
BpmI CTGGAG 1 cut(s) 30
Bpu1102I GCTNAGC 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 566
BsaBI GATNNNNATC 1 cut(s) 711
BsaJI CCNNGG 4 cut(s) 21, 198, 393, 900
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 358
Bse3DI GCAATG 1 cut(s) 276
Bse8I GATNNNNATC 1 cut(s) 711
BseAI TCCGGA 1 cut(s) 58
BseBI CCWGG 2 cut(s) 22, 458
BseDI CCNNGG 4 cut(s) 21, 198, 393, 900
BseGI GGATG 2 cut(s) 94, 827
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 2 cut(s) 65, 358
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 4 cut(s) 115, 332, 399, 534
BseRI GAGGAG 1 cut(s) 864
BseSI GKGCMC 2 cut(s) 352, 571
BseXI GCAGC 4 cut(s) 4, 497, 661, 836
BshNI GGYRCC 1 cut(s) 347
BsiHKAI GWGCWC 4 cut(s) 402, 456, 571, 733
BsiHKCI CYCGRG 1 cut(s) 758
BsiSI CCGG 1 cut(s) 59
BslI CCNNNNNNNGG 2 cut(s) 65, 358
BsmAI GTCTC 2 cut(s) 523, 602
BsoBI CYCGRG 1 cut(s) 758
Bsp1286I GDGCHC 5 cut(s) 352, 402, 456, 571, 733
Bsp13I TCCGGA 1 cut(s) 58
Bsp143I GATC 4 cut(s) 61, 192, 313, 735
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 1 cut(s) 393
BspACI CCGC 2 cut(s) 494, 831
BspCNI CTCAG 4 cut(s) 114, 331, 400, 533
BspEI TCCGGA 1 cut(s) 58
BspLI GGNNCC 2 cut(s) 70, 349
BspPI GGATC 3 cut(s) 69, 308, 730
BspT107I GGYRCC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 276
BssECI CCNNGG 4 cut(s) 21, 198, 393, 900
BssMI GATC 4 cut(s) 61, 192, 313, 735
BssT1I CCWWGG 3 cut(s) 198, 393, 900
Bst2UI CCWGG 2 cut(s) 22, 458
Bst6I CTCTTC 1 cut(s) 600
BstAPI GCANNNNNTGC 2 cut(s) 383, 533
BstBAI YACGTR 1 cut(s) 566
BstC8I GCNNGC 3 cut(s) 429, 892, 896
BstDEI CTNAG 5 cut(s) 101, 318, 408, 520, 575
BstDSI CCRYGG 1 cut(s) 393
BstF5I GGATG 2 cut(s) 94, 827
BstHHI GCGC 1 cut(s) 824
BstKTI GATC 4 cut(s) 64, 195, 316, 738
BstMAI GTCTC 2 cut(s) 523, 602
BstMBI GATC 4 cut(s) 61, 192, 313, 735
BstMWI GCNNNNNNNGC 5 cut(s) 23, 383, 533, 566, 830
BstNI CCWGG 2 cut(s) 22, 458
BstNSI RCATGY 1 cut(s) 536
BstSCI CCNGG 2 cut(s) 20, 456
BstSFI CTRYAG 1 cut(s) 181
BstSLI GKGCMC 2 cut(s) 352, 571
BstV1I GCAGC 4 cut(s) 4, 497, 661, 836
BstV2I GAAGAC 1 cut(s) 500
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BtgI CCRYGG 1 cut(s) 393
BtsCI GGATG 2 cut(s) 94, 827
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 2 cut(s) 402, 731
Cac8I GCNNGC 3 cut(s) 429, 892, 896
CaiI CAGNNNCTG 1 cut(s) 917
CfoI GCGC 1 cut(s) 824
Cfr13I GGNCC 1 cut(s) 502
CviAII CATG 5 cut(s) 282, 293, 394, 533, 740
DdeI CTNAG 5 cut(s) 101, 318, 408, 520, 575
DpnI GATC 4 cut(s) 63, 194, 315, 737
DpnII GATC 4 cut(s) 61, 192, 313, 735
Eam1104I CTCTTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 600
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 3 cut(s) 198, 393, 900
Eco24I GRGCYC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 502
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 758
EcoICRI GAGCTC 1 cut(s) 400
EcoRII CCWGG 2 cut(s) 20, 456
EcoT14I CCWWGG 3 cut(s) 198, 393, 900
EcoT22I ATGCAT 1 cut(s) 745
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 3 cut(s) 198, 393, 900
FaeI CATG 5 cut(s) 285, 296, 397, 536, 743
FalI AAGNNNNNCTT 2 cut(s) 614, 646
FatI CATG 5 cut(s) 281, 292, 393, 532, 739
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 18, 486, 675, 825
FokI GGATG 2 cut(s) 81, 814
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 4 cut(s) 18, 486, 675, 825
FspBI CTAG 4 cut(s) 342, 449, 626, 931
GlaI GCGC 1 cut(s) 823
GluI GCNGC 4 cut(s) 18, 486, 675, 825
GsuI CTGGAG 1 cut(s) 30
HapII CCGG 1 cut(s) 59
HhaI GCGC 1 cut(s) 824
Hin1II CATG 5 cut(s) 285, 296, 397, 536, 743
Hin6I GCGC 1 cut(s) 822
HinP1I GCGC 1 cut(s) 822
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 425, 861
HinfI GANTC 6 cut(s) 7, 55, 516, 712, 761, 855
HpaII CCGG 1 cut(s) 59
HphI GGTGA 1 cut(s) 270
Hpy166II GTNNAC 3 cut(s) 6, 290, 569
Hpy188I TCNGA 4 cut(s) 104, 309, 321, 523
Hpy188III TCNNGA 6 cut(s) 47, 59, 151, 449, 694, 758
Hpy8I GTNNAC 3 cut(s) 6, 290, 569
HpyAV CCTTC 3 cut(s) 352, 412, 804
HpyCH4IV ACGT 3 cut(s) 565, 592, 684
HpyCH4V TGCA 7 cut(s) 218, 269, 386, 431, 527, 569, 743
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 383, 533, 566, 830
HpyF3I CTNAG 5 cut(s) 101, 318, 408, 520, 575
HpySE526I ACGT 3 cut(s) 565, 592, 684
Hsp92II CATG 5 cut(s) 285, 296, 397, 536, 743
HspAI GCGC 1 cut(s) 822
Kpn2I TCCGGA 1 cut(s) 58
Kzo9I GATC 4 cut(s) 61, 192, 313, 735
LmnI GCTCC 3 cut(s) 261, 397, 793
LpnPI CCDG 8 cut(s) 7, 34, 60, 72, 365, 443, 470, 782
Lsp1109I GCAGC 4 cut(s) 4, 497, 661, 836
LweI GCATC 7 cut(s) 103, 144, 373, 514, 752, 836, 842
MaeI CTAG 4 cut(s) 342, 449, 626, 931
MaeII ACGT 3 cut(s) 565, 592, 684
MalI GATC 4 cut(s) 63, 194, 315, 737
MboI GATC 4 cut(s) 61, 192, 313, 735
MboII GAAGA 9 cut(s) 367, 392, 487, 490, 500, 617, 671, 674, 738
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 5 cut(s) 352, 402, 456, 571, 733
MluCI AATT 3 cut(s) 120, 132, 689
MlyI GAGTC 3 cut(s) 49, 525, 770
Mph1103I ATGCAT 1 cut(s) 745
MroI TCCGGA 1 cut(s) 58
MseI TTAA 2 cut(s) 213, 669
MslI CAYNNNNRTG 1 cut(s) 537
MspA1I CMGCKG 2 cut(s) 17, 488
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 2 cut(s) 22, 458
MvaI CCWGG 2 cut(s) 22, 458
MwoI GCNNNNNNNGC 5 cut(s) 23, 383, 533, 566, 830
NcoI CCATGG 1 cut(s) 393
NdeII GATC 4 cut(s) 61, 192, 313, 735
NlaIII CATG 5 cut(s) 285, 296, 397, 536, 743
NlaIV GGNNCC 2 cut(s) 70, 349
NsiI ATGCAT 1 cut(s) 745
NspI RCATGY 1 cut(s) 536
PfeI GAWTC 2 cut(s) 712, 855
PkrI GCNGC 4 cut(s) 19, 487, 676, 826
PleI GAGTC 3 cut(s) 49, 524, 769
PpsI GAGTC 3 cut(s) 49, 524, 769
Ppu21I YACGTR 1 cut(s) 566
Psp124BI GAGCTC 1 cut(s) 402
Psp6I CCWGG 2 cut(s) 20, 456
PspGI CCWGG 2 cut(s) 20, 456
PspN4I GGNNCC 2 cut(s) 70, 349
PspPI GGNCC 1 cut(s) 502
PstNI CAGNNNCTG 1 cut(s) 917
PsuI RGATCY 1 cut(s) 313
PvuII CAGCTG 2 cut(s) 17, 488
RseI CAYNNNNRTG 1 cut(s) 537
SacI GAGCTC 1 cut(s) 402
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 213, 669
SatI GCNGC 4 cut(s) 18, 486, 675, 825
Sau3AI GATC 4 cut(s) 61, 192, 313, 735
Sau96I GGNCC 1 cut(s) 502
SchI GAGTC 3 cut(s) 49, 525, 770
ScrFI CCNGG 2 cut(s) 22, 458
SduI GDGCHC 5 cut(s) 352, 402, 456, 571, 733
SfaNI GCATC 7 cut(s) 103, 144, 373, 514, 752, 836, 842
SfcI CTRYAG 1 cut(s) 181
SinI GGWCC 1 cut(s) 502
SmiMI CAYNNNNRTG 1 cut(s) 537
SpeI ACTAGT 1 cut(s) 341
Sse9I AATT 3 cut(s) 120, 132, 689
SsiI CCGC 2 cut(s) 494, 831
SspMI CTAG 4 cut(s) 342, 449, 626, 931
SstI GAGCTC 1 cut(s) 402
StyD4I CCNGG 2 cut(s) 20, 456
StyI CCWWGG 3 cut(s) 198, 393, 900
TaiI ACGT 3 cut(s) 568, 595, 687
TaqI TCGA 5 cut(s) 5, 150, 208, 715, 776
TasI AATT 3 cut(s) 120, 132, 689
TfiI GAWTC 2 cut(s) 712, 855
Tru1I TTAA 2 cut(s) 213, 669
Tru9I TTAA 2 cut(s) 213, 669
TscAI CASTG 2 cut(s) 409, 738
TseI GCWGC 4 cut(s) 17, 485, 674, 824
TspDTI ATGAA 4 cut(s) 287, 387, 855, 897
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 2 cut(s) 409, 738
VneI GTGCAC 1 cut(s) 567
VpaK11BI GGWCC 1 cut(s) 502
XapI RAATTY 2 cut(s) 132, 689
XbaI TCTAGA 1 cut(s) 448
XceI RCATGY 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 4 cut(s) 342, 449, 626, 931
Zsp2I ATGCAT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.