FvH4_1g20000

Coiled-coil domain-containing protein 130-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006850
Physical Location & Seq
Forward (+)
12114065 .. 12114837
773 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20000.t1

Sequence Viewer

Length: 696 bp
ATGCCATACAACATATGGTGCAGTGGGTGCAATTCTATGATCGCAAAGGGTGTTCGGTTCAACGCTGAAAAAAAGCATGTAGGAAACTACTATTCAACAAAGATATGGAGCTTCACCATGAAGGCTGCATGCTGCAAAGAGGAGATTGTTATGCAGACAGATCCGAAGAATTGTCGGTATGTGATTGTTAGTGGTGCTCAACAAAAGACTGAGGATTTTGACATTGAGGATGCAGAAACCCTTGCACTTCCTACAGATGATGAAAGAGGTATGCTGGCAGATCCATTTTATCGTCTTGAACATCAAGAAAAGGATCTGCAAAAGAAGAAGGAAGCCGAGCCAATGCTAGTCCGCTTACGGAGAATTTCTGACCAGAGGCATTCAGACGAGTATTCCCTTAACAAGTCCCTGAGGGCCAAACTTAGATCTCAAAAGAAGAAGAGGCTGCTTCAAGGAGAATGGGCATCAATATACGACTGCTTCCAGCGGCAGAAGAGGACGCTGCTGCTGCGTCCCAGGTCATCTGGGTTTCCCGTGGCCAATAAGAAAATTACGGGACTGGAAACTAAGAGAAGGAAAATAAGTGCTGCTGCTGCATATATACTTTCCGGGAGATTTAAGCCTTCATCATGGTCGCAGAATTCTGTTTCTTCAAGCAGGCCCAAGTTCACTCCAGTCTTAGCGAGACGATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

232

Amino Acids

26.76

Weight (kDa)

9.87

Isoelectric Point (pI)

56.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 1 - 151 1e-44 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 352, 487
AclWI GGATC 3 cut(s) 155, 275, 321
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 2 cut(s) 363, 640
AgsI TTSAA 5 cut(s) 61, 96, 299, 452, 654
AjnI CCWGG 1 cut(s) 515
AluBI AGCT 1 cut(s) 111
AluI AGCT 1 cut(s) 111
Alw21I GWGCWC 1 cut(s) 199
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 3 cut(s) 155, 275, 321
AoxI GGCC 3 cut(s) 414, 537, 659
ApeKI GCWGC 9 cut(s) 125, 132, 445, 502, 505, 508, 587, 590, 593
ApoI RAATTY 2 cut(s) 363, 640
AspS9I GGNCC 2 cut(s) 414, 660
AsuC2I CCSGG 1 cut(s) 610
AsuHPI GGTGA 1 cut(s) 106
AxyI CCTNAGG 1 cut(s) 410
BalI TGGCCA 1 cut(s) 539
Bbv12I GWGCWC 1 cut(s) 199
BbvI GCAGC 9 cut(s) 112, 119, 432, 489, 492, 495, 574, 577, 580
BciT130I CCWGG 1 cut(s) 517
BcnI CCSGG 1 cut(s) 610
BcoDI GTCTC 1 cut(s) 679
BfaI CTAG 1 cut(s) 347
BfmI CTRYAG 1 cut(s) 252
BglII AGATCT 1 cut(s) 425
Bme1390I CCNGG 2 cut(s) 517, 610
BmgT120I GGNCC 2 cut(s) 414, 660
BmrFI CCNGG 2 cut(s) 517, 610
BmsI GCATC 2 cut(s) 220, 473
BpmI CTGGAG 1 cut(s) 657
BpuMI CCSGG 1 cut(s) 610
BsaJI CCNNGG 2 cut(s) 515, 534
Bse1I ACTGG 2 cut(s) 564, 674
Bse21I CCTNAGG 1 cut(s) 410
BseBI CCWGG 1 cut(s) 517
BseDI CCNNGG 2 cut(s) 515, 534
BseGI GGATG 1 cut(s) 235
BseMII CTCAG 2 cut(s) 201, 401
BseNI ACTGG 2 cut(s) 564, 674
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 9 cut(s) 112, 119, 432, 489, 492, 495, 574, 577, 580
BsgI GTGCAG 1 cut(s) 40
BshFI GGCC 3 cut(s) 416, 539, 661
BsiHKAI GWGCWC 1 cut(s) 199
BsiSI CCGG 1 cut(s) 609
BslFI GGGAC 3 cut(s) 391, 498, 570
BsmAI GTCTC 1 cut(s) 679
BsmBI CGTCTC 1 cut(s) 679
BsmFI GGGAC 3 cut(s) 391, 498, 570
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 3 cut(s) 416, 539, 661
Bsp1286I GDGCHC 1 cut(s) 199
Bsp143I GATC 5 cut(s) 39, 160, 280, 313, 425
BspACI CCGC 2 cut(s) 352, 487
BspANI GGCC 3 cut(s) 416, 539, 661
BspCNI CTCAG 2 cut(s) 202, 402
BspPI GGATC 3 cut(s) 155, 275, 321
BsrI ACTGG 2 cut(s) 564, 674
BssECI CCNNGG 2 cut(s) 515, 534
BssMI GATC 5 cut(s) 39, 160, 280, 313, 425
Bst2UI CCWGG 1 cut(s) 517
Bst6I CTCTTC 2 cut(s) 434, 488
BstAPI GCANNNNNTGC 1 cut(s) 27
BstC8I GCNNGC 3 cut(s) 130, 276, 659
BstDEI CTNAG 5 cut(s) 210, 410, 422, 567, 679
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 5 cut(s) 42, 163, 283, 316, 428
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 5 cut(s) 39, 160, 280, 313, 425
BstMWI GCNNNNNNNGC 3 cut(s) 27, 508, 593
BstNI CCWGG 1 cut(s) 517
BstNSI RCATGY 2 cut(s) 80, 132
BstSCI CCNGG 2 cut(s) 515, 608
BstSFI CTRYAG 1 cut(s) 252
BstV1I GCAGC 9 cut(s) 112, 119, 432, 489, 492, 495, 574, 577, 580
BstX2I RGATCY 4 cut(s) 160, 280, 313, 425
BstYI RGATCY 4 cut(s) 160, 280, 313, 425
Bsu36I CCTNAGG 1 cut(s) 410
BsuRI GGCC 3 cut(s) 416, 539, 661
BtgI CCRYGG 1 cut(s) 534
BtsCI GGATG 1 cut(s) 235
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 1 cut(s) 28
Cac8I GCNNGC 3 cut(s) 130, 276, 659
Cfr13I GGNCC 2 cut(s) 414, 660
CseI GACGC 2 cut(s) 500, 508
CviAII CATG 4 cut(s) 77, 118, 129, 630
CviJI RGCY 9 cut(s) 111, 125, 335, 340, 416, 445, 539, 622, 661
CviKI_1 RGCY 9 cut(s) 111, 125, 335, 340, 416, 445, 539, 622, 661
DdeI CTNAG 5 cut(s) 210, 410, 422, 567, 679
DpnI GATC 5 cut(s) 41, 162, 282, 315, 427
DpnII GATC 5 cut(s) 39, 160, 280, 313, 425
EaeI YGGCCR 1 cut(s) 537
Eam1104I CTCTTC 2 cut(s) 434, 488
EarI CTCTTC 2 cut(s) 434, 488
Eco81I CCTNAGG 1 cut(s) 410
EcoRI GAATTC 1 cut(s) 640
EcoRII CCWGG 1 cut(s) 515
Esp3I CGTCTC 1 cut(s) 679
FaeI CATG 4 cut(s) 80, 121, 132, 633
FaqI GGGAC 3 cut(s) 391, 498, 570
FatI CATG 4 cut(s) 76, 117, 128, 629
FauNDI CATATG 1 cut(s) 14
FokI GGATG 1 cut(s) 242
FspBI CTAG 1 cut(s) 347
GsuI CTGGAG 1 cut(s) 657
HaeIII GGCC 3 cut(s) 416, 539, 661
HapII CCGG 1 cut(s) 609
HgaI GACGC 2 cut(s) 500, 508
Hin1II CATG 4 cut(s) 80, 121, 132, 633
HpaII CCGG 1 cut(s) 609
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 669
Hpy188I TCNGA 3 cut(s) 165, 370, 385
Hpy188III TCNNGA 2 cut(s) 296, 305
Hpy8I GTNNAC 1 cut(s) 669
HpyAV CCTTC 4 cut(s) 115, 322, 567, 633
HpyCH4V TGCA 9 cut(s) 21, 30, 128, 135, 154, 233, 245, 319, 596
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 508, 593
HpyF3I CTNAG 5 cut(s) 210, 410, 422, 567, 679
Hsp92II CATG 4 cut(s) 80, 121, 132, 633
Kzo9I GATC 5 cut(s) 39, 160, 280, 313, 425
LmnI GCTCC 1 cut(s) 108
Lsp1109I GCAGC 9 cut(s) 112, 119, 432, 489, 492, 495, 574, 577, 580
LweI GCATC 2 cut(s) 220, 473
MaeI CTAG 1 cut(s) 347
MalI GATC 5 cut(s) 41, 162, 282, 315, 427
MboI GATC 5 cut(s) 39, 160, 280, 313, 425
MboII GAAGA 6 cut(s) 178, 337, 448, 451, 505, 642
MflI RGATCY 4 cut(s) 160, 280, 313, 425
MhlI GDGCHC 1 cut(s) 199
MlsI TGGCCA 1 cut(s) 539
MluCI AATT 5 cut(s) 31, 169, 363, 549, 640
MluNI TGGCCA 1 cut(s) 539
MnlI CCTC 8 cut(s) 133, 205, 220, 260, 369, 405, 435, 489
Mox20I TGGCCA 1 cut(s) 539
MscI TGGCCA 1 cut(s) 539
MseI TTAA 2 cut(s) 399, 618
Msp20I TGGCCA 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 487
MspI CCGG 1 cut(s) 609
MspR9I CCNGG 2 cut(s) 517, 610
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 1 cut(s) 517
MwoI GCNNNNNNNGC 3 cut(s) 27, 508, 593
NciI CCSGG 1 cut(s) 610
NdeI CATATG 1 cut(s) 14
NdeII GATC 5 cut(s) 39, 160, 280, 313, 425
NlaIII CATG 4 cut(s) 80, 121, 132, 633
NmeAIII GCCGAG 1 cut(s) 361
NspI RCATGY 2 cut(s) 80, 132
PaeI GCATGC 1 cut(s) 132
PctI GAATGC 1 cut(s) 379
PfoI TCCNGGA 1 cut(s) 608
Psp6I CCWGG 1 cut(s) 515
PspGI CCWGG 1 cut(s) 515
PspPI GGNCC 2 cut(s) 414, 660
PsuI RGATCY 4 cut(s) 160, 280, 313, 425
SaqAI TTAA 2 cut(s) 399, 618
Sau3AI GATC 5 cut(s) 39, 160, 280, 313, 425
Sau96I GGNCC 2 cut(s) 414, 660
ScrFI CCNGG 2 cut(s) 517, 610
SduI GDGCHC 1 cut(s) 199
SetI ASST 3 cut(s) 113, 271, 521
SfaNI GCATC 2 cut(s) 220, 473
SfcI CTRYAG 1 cut(s) 252
SphI GCATGC 1 cut(s) 132
Sse9I AATT 5 cut(s) 31, 169, 363, 549, 640
SsiI CCGC 2 cut(s) 352, 487
SspMI CTAG 1 cut(s) 347
StyD4I CCNGG 2 cut(s) 515, 608
TasI AATT 5 cut(s) 31, 169, 363, 549, 640
TauI GCSGC 1 cut(s) 490
Tru1I TTAA 2 cut(s) 399, 618
Tru9I TTAA 2 cut(s) 399, 618
TscAI CASTG 1 cut(s) 28
TseI GCWGC 9 cut(s) 125, 132, 445, 502, 505, 508, 587, 590, 593
TspDTI ATGAA 3 cut(s) 134, 276, 615
TspGWI ACGGA 1 cut(s) 373
TspRI CASTG 1 cut(s) 28
XapI RAATTY 2 cut(s) 363, 640
XceI RCATGY 2 cut(s) 80, 132
XcmI CCANNNNNNNNNTGG 1 cut(s) 12
XspI CTAG 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.