pycom13g04140

Coiled-coil domain-containing protein 130-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006850
Physical Location & Seq
Reverse (-)
2787071 .. 2790221
3151 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g04140.1

Sequence Viewer

Length: 933 bp
ATGTCTACACTTGCAGCTGCTAGGGCAGACAACTTTTATTATCCCCCAGAATGGTCGCCAAAGAAGGGCTCCTTGAACAAGTTCAACGGTCAACATGCTCTGAGAGAGAGAGCAAGGAAATTGGACCAGGGAATTCTGATTATAAGGTTCGAGATGCCTTACCATATATGGTGCGGTGGTTGCAATTCTATGATTGCGAAGGGTGTTCGTTTTAATGCAGAAAAAAAGCAAGTTGGAAATTACTACTCTACAAAGATATGGAGCTTTACCATGAAGTCTCCATGCTGCAAACAAGAGATTGTTATTCAGACAGATCCGAAGAATTGCCAGTATGTGATTATTAGTGGGGCCACGCAAAAGAATGAGGAATTTGACGTTGAGGATGCGGAAACCTTTGAACTTCCTGCTGATGAAGAAAGAGGAAAGCTTGCGGATCCATTTTATCGTCTTGAACACCAAGAAGAGGATTTGCAAAAGAAGAAAGAAGCCGAGCCATTACTTGTTCGCCTACAGAGAATTTCTGATGACAGGCATTTAGATGACTATTCCCTCAACAAGGCCCTCAGAGCCAAACTTAGATCTCAAAAGAAAAGAGTTGCCGAAGAAGAGGCTGCTTCCAGGAAGATGGGTCTCGGTTTACGACTGCTTCCAGTGGCAGAGGAGGATGCTGCTGCTGCGTCGCGTGTGAAGTTCGTTTCCAAGTTTGAAAAAAATAGGAAGGATAAGCGAGCACTAATCAATGCGGCTTCAATTTTTCCTGGCGTATCTGGGACTTCTAAGAAGAGTTTGGAACTACAATCCAAGAGAAGAAGGATAAGTGCTGCTGCTGCATCCAACTTACTTACAGGGGGATTCAAGCCATCATCATGGTCCCAGAATGCGGTTTCTTCAGGCAGGCAAAATGGAATGTCAGTCACAGTGAGACGCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

311

Amino Acids

35.05

Weight (kDa)

9.65

Isoelectric Point (pI)

56.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 13 - 269 2e-61 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 143
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 682
AciI CCGC 5 cut(s) 174, 386, 431, 743, 881
AclWI GGATC 3 cut(s) 308, 428, 441
AcsI RAATTY 3 cut(s) 132, 368, 516
AcuI CTGAAG 1 cut(s) 873
AcyI GRCGYC 1 cut(s) 925
AfiI CCNNNNNNNGG 5 cut(s) 51, 65, 463, 556, 880
AgsI TTSAA 7 cut(s) 76, 85, 398, 452, 707, 750, 856
AjnI CCWGG 3 cut(s) 126, 617, 757
AluBI AGCT 3 cut(s) 17, 264, 427
AluI AGCT 3 cut(s) 17, 264, 427
Alw21I GWGCWC 1 cut(s) 733
Alw26I GTCTC 3 cut(s) 282, 635, 916
AlwI GGATC 3 cut(s) 308, 428, 441
AoxI GGCC 2 cut(s) 348, 558
ApoI RAATTY 3 cut(s) 132, 368, 516
Asp700I GAANNNNTTC 1 cut(s) 80
AspS9I GGNCC 4 cut(s) 124, 348, 559, 870
AvaII GGWCC 2 cut(s) 124, 870
BamHI GGATCC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 733
BccI CCATC 2 cut(s) 619, 868
BciT130I CCWGG 3 cut(s) 128, 619, 759
BcoDI GTCTC 3 cut(s) 282, 635, 916
BfaI CTAG 2 cut(s) 21, 931
BfmI CTRYAG 1 cut(s) 509
BglII AGATCT 1 cut(s) 578
Bme1390I CCNGG 3 cut(s) 128, 619, 759
Bme18I GGWCC 2 cut(s) 124, 870
BmgT120I GGNCC 4 cut(s) 124, 348, 559, 870
BmiI GGNNCC 4 cut(s) 70, 349, 435, 872
BmrFI CCNGG 3 cut(s) 128, 619, 759
BmsI GCATC 4 cut(s) 144, 373, 655, 839
BsaHI GRCGYC 1 cut(s) 925
BsaI GGTCTC 1 cut(s) 635
BsaJI CCNNGG 1 cut(s) 127
Bsc4I CCNNNNNNNGG 5 cut(s) 51, 65, 463, 556, 880
Bse1I ACTGG 2 cut(s) 328, 650
BseBI CCWGG 3 cut(s) 128, 619, 759
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 3 cut(s) 388, 670, 830
BseLI CCNNNNNNNGG 5 cut(s) 51, 65, 463, 556, 880
BseMII CTCAG 2 cut(s) 92, 577
BseNI ACTGG 2 cut(s) 328, 650
BseRI GAGGAG 1 cut(s) 674
Bsh1236I CGCG 1 cut(s) 682
BshFI GGCC 2 cut(s) 350, 560
BsiHKAI GWGCWC 1 cut(s) 733
BslFI GGGAC 2 cut(s) 784, 856
BslI CCNNNNNNNGG 5 cut(s) 51, 65, 463, 556, 880
BsmAI GTCTC 3 cut(s) 282, 635, 916
BsmBI CGTCTC 1 cut(s) 916
BsmFI GGGAC 2 cut(s) 784, 856
BsmI GAATGC 1 cut(s) 883
BsnI GGCC 2 cut(s) 350, 560
Bso31I GGTCTC 1 cut(s) 635
Bsp1286I GDGCHC 2 cut(s) 71, 733
Bsp143I GATC 3 cut(s) 313, 433, 578
BspACI CCGC 5 cut(s) 174, 386, 431, 743, 881
BspANI GGCC 2 cut(s) 350, 560
BspCNI CTCAG 2 cut(s) 93, 576
BspFNI CGCG 1 cut(s) 682
BspLI GGNNCC 4 cut(s) 70, 349, 435, 872
BspPI GGATC 3 cut(s) 308, 428, 441
BspTNI GGTCTC 1 cut(s) 635
BsrI ACTGG 2 cut(s) 328, 650
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 3 cut(s) 313, 433, 578
BssNI GRCGYC 1 cut(s) 925
Bst2UI CCWGG 3 cut(s) 128, 619, 759
Bst4CI ACNGT 2 cut(s) 89, 919
Bst6I CTCTTC 3 cut(s) 456, 600, 776
BstACI GRCGYC 1 cut(s) 925
BstC8I GCNNGC 3 cut(s) 429, 729, 896
BstDEI CTNAG 4 cut(s) 101, 563, 575, 777
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 3 cut(s) 388, 670, 830
BstFNI CGCG 1 cut(s) 682
BstKTI GATC 3 cut(s) 316, 436, 581
BstMAI GTCTC 3 cut(s) 282, 635, 916
BstMBI GATC 3 cut(s) 313, 433, 578
BstMWI GCNNNNNNNGC 5 cut(s) 23, 180, 566, 674, 827
BstNI CCWGG 3 cut(s) 128, 619, 759
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 3 cut(s) 126, 617, 757
BstSFI CTRYAG 1 cut(s) 509
BstUI CGCG 1 cut(s) 682
BstX2I RGATCY 3 cut(s) 313, 433, 578
BstXI CCANNNNNNTGG 2 cut(s) 625, 867
BstYI RGATCY 3 cut(s) 313, 433, 578
BsuRI GGCC 2 cut(s) 350, 560
BtsCI GGATG 3 cut(s) 388, 670, 830
BtsIMutI CAGTG 2 cut(s) 657, 924
Cac8I GCNNGC 3 cut(s) 429, 729, 896
Cfr13I GGNCC 4 cut(s) 124, 348, 559, 870
CseI GACGC 1 cut(s) 666
CviAII CATG 4 cut(s) 95, 271, 282, 867
DdeI CTNAG 4 cut(s) 101, 563, 575, 777
DpnI GATC 3 cut(s) 315, 435, 580
DpnII GATC 3 cut(s) 313, 433, 578
Eam1104I CTCTTC 3 cut(s) 456, 600, 776
EarI CTCTTC 3 cut(s) 456, 600, 776
Eco24I GRGCYC 1 cut(s) 71
Eco31I GGTCTC 1 cut(s) 635
Eco47I GGWCC 2 cut(s) 124, 870
Eco57I CTGAAG 1 cut(s) 873
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 559
EcoRI GAATTC 1 cut(s) 132
EcoRII CCWGG 3 cut(s) 126, 617, 757
EcoT38I GRGCYC 1 cut(s) 71
Esp3I CGTCTC 1 cut(s) 916
FaeI CATG 4 cut(s) 98, 274, 285, 870
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FaqI GGGAC 2 cut(s) 784, 856
FatI CATG 4 cut(s) 94, 270, 281, 866
FblI GTMKAC 1 cut(s) 5
FokI GGATG 3 cut(s) 395, 677, 817
FriOI GRGCYC 1 cut(s) 71
FspBI CTAG 2 cut(s) 21, 931
HaeIII GGCC 2 cut(s) 350, 560
HgaI GACGC 1 cut(s) 666
Hin1I GRCGYC 1 cut(s) 925
Hin1II CATG 4 cut(s) 98, 274, 285, 870
HincII GTYRAC 1 cut(s) 92
HindII GTYRAC 1 cut(s) 92
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 1 cut(s) 852
Hpy166II GTNNAC 3 cut(s) 6, 92, 638
Hpy188I TCNGA 6 cut(s) 102, 138, 309, 318, 523, 566
Hpy188III TCNNGA 2 cut(s) 151, 449
Hpy8I GTNNAC 3 cut(s) 6, 92, 638
Hpy99I CGWCG 1 cut(s) 682
HpyAV CCTTC 4 cut(s) 58, 193, 712, 804
HpyCH4III ACNGT 2 cut(s) 89, 919
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 6 cut(s) 14, 183, 218, 288, 472, 830
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 180, 566, 674, 827
HpyF3I CTNAG 4 cut(s) 101, 563, 575, 777
HpySE526I ACGT 1 cut(s) 375
Hsp92I GRCGYC 1 cut(s) 925
Hsp92II CATG 4 cut(s) 98, 274, 285, 870
Kzo9I GATC 3 cut(s) 313, 433, 578
LmnI GCTCC 2 cut(s) 74, 261
LweI GCATC 4 cut(s) 144, 373, 655, 839
MaeI CTAG 2 cut(s) 21, 931
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 1 cut(s) 913
MalI GATC 3 cut(s) 315, 435, 580
MboI GATC 3 cut(s) 313, 433, 578
MflI RGATCY 3 cut(s) 313, 433, 578
MhlI GDGCHC 2 cut(s) 71, 733
MluCI AATT 8 cut(s) 119, 132, 184, 238, 322, 368, 516, 750
MmeI TCCRAC 2 cut(s) 214, 858
MnlI CCTC 9 cut(s) 358, 373, 413, 457, 560, 572, 601, 652, 655
MroXI GAANNNNTTC 1 cut(s) 80
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 2 cut(s) 537, 865
MspA1I CMGCKG 1 cut(s) 17
MspR9I CCNGG 3 cut(s) 128, 619, 759
Mva1269I GAATGC 1 cut(s) 883
MvaI CCWGG 3 cut(s) 128, 619, 759
MvnI CGCG 1 cut(s) 682
MwoI GCNNNNNNNGC 5 cut(s) 23, 180, 566, 674, 827
NdeII GATC 3 cut(s) 313, 433, 578
NlaIII CATG 4 cut(s) 98, 274, 285, 870
NlaIV GGNNCC 4 cut(s) 70, 349, 435, 872
NmeAIII GCCGAG 1 cut(s) 514
NmuCI GTSAC 1 cut(s) 913
NspI RCATGY 1 cut(s) 98
PctI GAATGC 1 cut(s) 883
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 1 cut(s) 852
PfoI TCCNGGA 1 cut(s) 617
PsiI TTATAA 1 cut(s) 143
Psp6I CCWGG 3 cut(s) 126, 617, 757
PspGI CCWGG 3 cut(s) 126, 617, 757
PspN4I GGNNCC 4 cut(s) 70, 349, 435, 872
PspPI GGNCC 4 cut(s) 124, 348, 559, 870
PsuI RGATCY 3 cut(s) 313, 433, 578
PvuII CAGCTG 1 cut(s) 17
RseI CAYNNNNRTG 2 cut(s) 537, 865
SaqAI TTAA 1 cut(s) 213
Sau3AI GATC 3 cut(s) 313, 433, 578
Sau96I GGNCC 4 cut(s) 124, 348, 559, 870
ScrFI CCNGG 3 cut(s) 128, 619, 759
SduI GDGCHC 2 cut(s) 71, 733
SetI ASST 6 cut(s) 19, 149, 266, 378, 395, 429
SfaNI GCATC 4 cut(s) 144, 373, 655, 839
SfcI CTRYAG 1 cut(s) 509
SinI GGWCC 2 cut(s) 124, 870
SmiMI CAYNNNNRTG 2 cut(s) 537, 865
Sse9I AATT 8 cut(s) 119, 132, 184, 238, 322, 368, 516, 750
SsiI CCGC 5 cut(s) 174, 386, 431, 743, 881
SspMI CTAG 2 cut(s) 21, 931
StyD4I CCNGG 3 cut(s) 126, 617, 757
TaaI ACNGT 2 cut(s) 89, 919
TaiI ACGT 1 cut(s) 378
TaqI TCGA 1 cut(s) 150
TasI AATT 8 cut(s) 119, 132, 184, 238, 322, 368, 516, 750
TauI GCSGC 1 cut(s) 746
TfiI GAWTC 1 cut(s) 852
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 2 cut(s) 657, 924
TseFI GTSAC 1 cut(s) 913
Tsp45I GTSAC 1 cut(s) 913
TspDTI ATGAA 2 cut(s) 287, 426
TspRI CASTG 2 cut(s) 657, 924
VpaK11BI GGWCC 2 cut(s) 124, 870
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 3 cut(s) 132, 368, 516
XceI RCATGY 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 80
XspI CTAG 2 cut(s) 21, 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.