MD13G1047300.v1.1

Coiled-coil domain-containing protein 130-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006850
Physical Location & Seq
Reverse (-)
3323127 .. 3326696
3570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1047300.v1.1.491

Sequence Viewer

Length: 933 bp
ATGTCTACACTTGCAGCTGCTAGGGCAGACAACTTTTATTATCCCCCAGAATGGTCGCCAAAGAAGGGCTCCTTGAACAAGTTCAACGGTCAACATGCTCTGAGGGAGAGAGCGAGGAAATTGGACCAGGGGATTCTGATTATAAGGTTCGAGATGCCTTACCATATATGGTGCGGTGGTTGCAATTCTATGATTGCGAAGGGTGTTCGTTTTAATGCAGAAAAAAAGCAAGTTGGAAATTACTACTCTACAAAGATATGGAGCTTTACCATGAAGTCTCCATGCTGCAAACAAGAGATTGTTATTCAGACAGATCCGAAGAATTGCCAGTATGTGATTATTAGTGGGGCCACGCAAAAGAATGAGGAATTTGACGTTGAGGATGCAGAAACCTTTGAACTTCCTGCTGATGAAGAAAGAGGAAAGCTTGCGGATCCATTTTATCGTCTTGAACACCAAGAAGAGGATTTGCAAAAGAAGAAAGAAGCCGAGCCATTACTTGTTCGCCTACAGAGAATTTCTGATGACAGGCATTTAGATGACTATTCCCTCAACAAGGCCCTCAGAGCCAAACTTAGATCTCAAAAGAAAAGAGTTGCCGAAGAAGAGGCTGCTTCAAGGAAGATGGGTCTCGGTTTACGACTGCTTCCAGTGGCAGAGGAGGATGCTGCTGCTGCGTCGCGTGTGAAGTTCGTTTCCAAGTTAGAAAAAAATAGGAAGGATAAGCGAGCACTAATCAATGGGGCTTCAATTTTTCCTGGCGCATCTGGGACTTCTAAGAAGAGTTTGGAACTGCAATCTAAGAGAAGAAGGATAAGTGCTGCTGCTGCATCCAACTTACTTACAGGGGGATTCAAGCCATCATCATGGTCTCAGAATGCGGTTTCTTCAGGCAGGCAAAATGGAATGTCAGTCACAGTGAGACGCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

311

Amino Acids

34.95

Weight (kDa)

9.65

Isoelectric Point (pI)

56.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 13 - 271 2e-61 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 143
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 682
AciI CCGC 3 cut(s) 174, 431, 881
AclWI GGATC 3 cut(s) 308, 428, 441
AcsI RAATTY 2 cut(s) 368, 516
AcuI CTGAAG 1 cut(s) 873
AfiI CCNNNNNNNGG 4 cut(s) 51, 65, 463, 556
AgsI TTSAA 7 cut(s) 76, 85, 398, 452, 618, 750, 856
AjnI CCWGG 2 cut(s) 126, 757
AluBI AGCT 3 cut(s) 17, 264, 427
AluI AGCT 3 cut(s) 17, 264, 427
Alw21I GWGCWC 1 cut(s) 733
Alw26I GTCTC 4 cut(s) 282, 635, 876, 916
AlwI GGATC 3 cut(s) 308, 428, 441
AoxI GGCC 2 cut(s) 348, 558
ApoI RAATTY 2 cut(s) 368, 516
Asp700I GAANNNNTTC 1 cut(s) 80
AspLEI GCGC 1 cut(s) 764
AspS9I GGNCC 3 cut(s) 124, 348, 559
AvaII GGWCC 1 cut(s) 124
BamHI GGATCC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 733
BccI CCATC 2 cut(s) 619, 868
BciT130I CCWGG 2 cut(s) 128, 759
BcoDI GTCTC 4 cut(s) 282, 635, 876, 916
BfaI CTAG 2 cut(s) 21, 931
BfmI CTRYAG 1 cut(s) 509
BglII AGATCT 1 cut(s) 578
Bme1390I CCNGG 2 cut(s) 128, 759
Bme18I GGWCC 1 cut(s) 124
BmgT120I GGNCC 3 cut(s) 124, 348, 559
BmiI GGNNCC 3 cut(s) 70, 349, 435
BmrFI CCNGG 2 cut(s) 128, 759
BmsI GCATC 5 cut(s) 144, 373, 655, 773, 839
BsaI GGTCTC 2 cut(s) 635, 876
BsaJI CCNNGG 1 cut(s) 127
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 65, 463, 556
Bse1I ACTGG 2 cut(s) 328, 650
BseBI CCWGG 2 cut(s) 128, 759
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 3 cut(s) 388, 670, 830
BseLI CCNNNNNNNGG 4 cut(s) 51, 65, 463, 556
BseMII CTCAG 3 cut(s) 92, 577, 887
BseNI ACTGG 2 cut(s) 328, 650
BseRI GAGGAG 1 cut(s) 674
Bsh1236I CGCG 1 cut(s) 682
BshFI GGCC 2 cut(s) 350, 560
BsiHKAI GWGCWC 1 cut(s) 733
BslFI GGGAC 1 cut(s) 784
BslI CCNNNNNNNGG 4 cut(s) 51, 65, 463, 556
BsmAI GTCTC 4 cut(s) 282, 635, 876, 916
BsmBI CGTCTC 1 cut(s) 916
BsmFI GGGAC 1 cut(s) 784
BsmI GAATGC 1 cut(s) 883
BsnI GGCC 2 cut(s) 350, 560
Bso31I GGTCTC 2 cut(s) 635, 876
Bsp1286I GDGCHC 2 cut(s) 71, 733
Bsp143I GATC 3 cut(s) 313, 433, 578
BspACI CCGC 3 cut(s) 174, 431, 881
BspANI GGCC 2 cut(s) 350, 560
BspCNI CTCAG 3 cut(s) 93, 576, 886
BspFNI CGCG 1 cut(s) 682
BspLI GGNNCC 3 cut(s) 70, 349, 435
BspPI GGATC 3 cut(s) 308, 428, 441
BspTNI GGTCTC 2 cut(s) 635, 876
BsrI ACTGG 2 cut(s) 328, 650
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 3 cut(s) 313, 433, 578
Bst2UI CCWGG 2 cut(s) 128, 759
Bst4CI ACNGT 2 cut(s) 89, 919
Bst6I CTCTTC 3 cut(s) 456, 600, 776
BstC8I GCNNGC 3 cut(s) 429, 729, 896
BstDEI CTNAG 6 cut(s) 101, 563, 575, 777, 801, 873
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 3 cut(s) 388, 670, 830
BstFNI CGCG 1 cut(s) 682
BstHHI GCGC 1 cut(s) 764
BstKTI GATC 3 cut(s) 316, 436, 581
BstMAI GTCTC 4 cut(s) 282, 635, 876, 916
BstMBI GATC 3 cut(s) 313, 433, 578
BstMWI GCNNNNNNNGC 5 cut(s) 23, 180, 566, 674, 827
BstNI CCWGG 2 cut(s) 128, 759
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 126, 757
BstSFI CTRYAG 1 cut(s) 509
BstUI CGCG 1 cut(s) 682
BstX2I RGATCY 3 cut(s) 313, 433, 578
BstXI CCANNNNNNTGG 1 cut(s) 867
BstYI RGATCY 3 cut(s) 313, 433, 578
BsuRI GGCC 2 cut(s) 350, 560
BtsCI GGATG 3 cut(s) 388, 670, 830
BtsIMutI CAGTG 2 cut(s) 657, 924
Cac8I GCNNGC 3 cut(s) 429, 729, 896
CfoI GCGC 1 cut(s) 764
Cfr13I GGNCC 3 cut(s) 124, 348, 559
CseI GACGC 1 cut(s) 666
CviAII CATG 4 cut(s) 95, 271, 282, 867
DdeI CTNAG 6 cut(s) 101, 563, 575, 777, 801, 873
DpnI GATC 3 cut(s) 315, 435, 580
DpnII GATC 3 cut(s) 313, 433, 578
Eam1104I CTCTTC 3 cut(s) 456, 600, 776
EarI CTCTTC 3 cut(s) 456, 600, 776
Eco24I GRGCYC 1 cut(s) 71
Eco31I GGTCTC 2 cut(s) 635, 876
Eco47I GGWCC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 873
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 559
EcoRII CCWGG 2 cut(s) 126, 757
EcoT38I GRGCYC 1 cut(s) 71
Esp3I CGTCTC 1 cut(s) 916
FaeI CATG 4 cut(s) 98, 274, 285, 870
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FaqI GGGAC 1 cut(s) 784
FatI CATG 4 cut(s) 94, 270, 281, 866
FblI GTMKAC 1 cut(s) 5
FokI GGATG 3 cut(s) 395, 677, 817
FriOI GRGCYC 1 cut(s) 71
FspBI CTAG 2 cut(s) 21, 931
GlaI GCGC 1 cut(s) 763
HaeIII GGCC 2 cut(s) 350, 560
HgaI GACGC 1 cut(s) 666
HhaI GCGC 1 cut(s) 764
Hin1II CATG 4 cut(s) 98, 274, 285, 870
Hin6I GCGC 1 cut(s) 762
HinP1I GCGC 1 cut(s) 762
HincII GTYRAC 1 cut(s) 92
HindII GTYRAC 1 cut(s) 92
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 2 cut(s) 133, 852
Hpy166II GTNNAC 3 cut(s) 6, 92, 638
Hpy188I TCNGA 7 cut(s) 102, 138, 309, 318, 523, 566, 876
Hpy188III TCNNGA 2 cut(s) 151, 449
Hpy8I GTNNAC 3 cut(s) 6, 92, 638
Hpy99I CGWCG 1 cut(s) 682
HpyAV CCTTC 4 cut(s) 58, 193, 712, 804
HpyCH4III ACNGT 2 cut(s) 89, 919
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 8 cut(s) 14, 183, 218, 288, 386, 472, 796, 830
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 180, 566, 674, 827
HpyF3I CTNAG 6 cut(s) 101, 563, 575, 777, 801, 873
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 4 cut(s) 98, 274, 285, 870
HspAI GCGC 1 cut(s) 762
Kzo9I GATC 3 cut(s) 313, 433, 578
LmnI GCTCC 3 cut(s) 74, 261, 932
LweI GCATC 5 cut(s) 144, 373, 655, 773, 839
MaeI CTAG 2 cut(s) 21, 931
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 1 cut(s) 913
MalI GATC 3 cut(s) 315, 435, 580
MboI GATC 3 cut(s) 313, 433, 578
MflI RGATCY 3 cut(s) 313, 433, 578
MhlI GDGCHC 2 cut(s) 71, 733
MluCI AATT 7 cut(s) 119, 184, 238, 322, 368, 516, 750
MmeI TCCRAC 2 cut(s) 214, 858
MroXI GAANNNNTTC 1 cut(s) 80
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 2 cut(s) 537, 865
MspA1I CMGCKG 1 cut(s) 17
MspR9I CCNGG 2 cut(s) 128, 759
Mva1269I GAATGC 1 cut(s) 883
MvaI CCWGG 2 cut(s) 128, 759
MvnI CGCG 1 cut(s) 682
MwoI GCNNNNNNNGC 5 cut(s) 23, 180, 566, 674, 827
NdeII GATC 3 cut(s) 313, 433, 578
NlaIII CATG 4 cut(s) 98, 274, 285, 870
NlaIV GGNNCC 3 cut(s) 70, 349, 435
NmeAIII GCCGAG 1 cut(s) 514
NmuCI GTSAC 1 cut(s) 913
NspI RCATGY 1 cut(s) 98
PctI GAATGC 1 cut(s) 883
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 2 cut(s) 133, 852
PsiI TTATAA 1 cut(s) 143
Psp6I CCWGG 2 cut(s) 126, 757
PspGI CCWGG 2 cut(s) 126, 757
PspN4I GGNNCC 3 cut(s) 70, 349, 435
PspPI GGNCC 3 cut(s) 124, 348, 559
PsuI RGATCY 3 cut(s) 313, 433, 578
PvuII CAGCTG 1 cut(s) 17
RseI CAYNNNNRTG 2 cut(s) 537, 865
SaqAI TTAA 1 cut(s) 213
Sau3AI GATC 3 cut(s) 313, 433, 578
Sau96I GGNCC 3 cut(s) 124, 348, 559
ScrFI CCNGG 2 cut(s) 128, 759
SduI GDGCHC 2 cut(s) 71, 733
SetI ASST 6 cut(s) 19, 149, 266, 378, 395, 429
SfaNI GCATC 5 cut(s) 144, 373, 655, 773, 839
SfcI CTRYAG 1 cut(s) 509
SinI GGWCC 1 cut(s) 124
SmiMI CAYNNNNRTG 2 cut(s) 537, 865
Sse9I AATT 7 cut(s) 119, 184, 238, 322, 368, 516, 750
SsiI CCGC 3 cut(s) 174, 431, 881
SspMI CTAG 2 cut(s) 21, 931
StyD4I CCNGG 2 cut(s) 126, 757
TaaI ACNGT 2 cut(s) 89, 919
TaiI ACGT 1 cut(s) 378
TaqI TCGA 1 cut(s) 150
TasI AATT 7 cut(s) 119, 184, 238, 322, 368, 516, 750
TfiI GAWTC 2 cut(s) 133, 852
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 2 cut(s) 657, 924
TseFI GTSAC 1 cut(s) 913
Tsp45I GTSAC 1 cut(s) 913
TspDTI ATGAA 2 cut(s) 287, 426
TspRI CASTG 2 cut(s) 657, 924
VpaK11BI GGWCC 1 cut(s) 124
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 2 cut(s) 368, 516
XceI RCATGY 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 80
XspI CTAG 2 cut(s) 21, 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.