RLG00000017940

Coiled-coil domain-containing protein 130-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006850
Physical Location & Seq
Forward (+)
23278282 .. 23279031
750 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017940

Sequence Viewer

Length: 552 bp
ATGGAGGCCAAATCAGGGCTCCTTGGACAAGTTCCTTGGTCAACATGCTTTGAGAGAGAGAGCACGAAAATTGGACCAAGGTACTCTGATTATAAGTGTGGTGCTCAACAAAAGACCGAGGATTTTGACAATGAGGATGCGGAAACCCTTGCGCTTCCTGCAGATGAAGAAAGAGGTATGCTGGCGGATCCATTTTATCGTCTTGAACATCAAGAAAAGGATTCGCAAAAGAAGAAGCAAGCCGAGCCAATGCTACTCCTCTTAAAGAGAGATCGAATTTCCGACCAGAGGCATTCAGATGACTATTCCCTTAACAAGTCCCTGAGGCCCAAACTTAGATCTCAAAAGAAAAGAGTCGCTGAAGAAGAGGCTGCTTCAAGGAGAGTGGGCCTCAATATACGACTGCTTCCAGTAGCAGAAGAGGACACTGCCCCTGCGTCGCGGGTGAAGTTCTCGACCAAATTTGACAAAAGTAGAAGGGATAAGCGAGCTTTGATTAATGCTTCTTCAATCTTTCCTGGGTCATTGATCTACGTTTCCCATCGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

184

Amino Acids

21.02

Weight (kDa)

9.16

Isoelectric Point (pI)

60.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 16 - 161 5.4e-10 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 93
AccII CGCG 1 cut(s) 442
AciI CCGC 3 cut(s) 140, 185, 442
AclWI GGATC 2 cut(s) 182, 195
AcsI RAATTY 2 cut(s) 276, 461
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 2 cut(s) 15, 288
AgsI TTSAA 3 cut(s) 206, 378, 510
AjnI CCWGG 1 cut(s) 517
AluBI AGCT 1 cut(s) 491
AluI AGCT 1 cut(s) 491
Alw21I GWGCWC 2 cut(s) 65, 106
AlwI GGATC 2 cut(s) 182, 195
AoxI GGCC 3 cut(s) 6, 326, 388
ApeKI GCWGC 1 cut(s) 371
ApoI RAATTY 2 cut(s) 276, 461
AseI ATTAAT 1 cut(s) 498
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 3 cut(s) 74, 327, 388
AsuHPI GGTGA 1 cut(s) 457
AvaII GGWCC 1 cut(s) 74
AxyI CCTNAGG 1 cut(s) 323
BamHI GGATCC 1 cut(s) 187
BanII GRGCYC 1 cut(s) 21
Bbv12I GWGCWC 2 cut(s) 65, 106
BbvI GCAGC 1 cut(s) 358
BccI CCATC 1 cut(s) 549
BciT130I CCWGG 1 cut(s) 519
BfmI CTRYAG 1 cut(s) 159
BglII AGATCT 1 cut(s) 338
BisI GCNGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 373
Bme1390I CCNGG 1 cut(s) 519
Bme18I GGWCC 1 cut(s) 74
BmgT120I GGNCC 3 cut(s) 74, 327, 388
BmiI GGNNCC 2 cut(s) 20, 189
BmrFI CCNGG 1 cut(s) 519
BmsI GCATC 1 cut(s) 127
BplI GAGNNNNNCTC 2 cut(s) 375, 407
BsaJI CCNNGG 5 cut(s) 22, 35, 77, 117, 518
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 288
Bse1I ACTGG 1 cut(s) 410
Bse21I CCTNAGG 1 cut(s) 323
BseBI CCWGG 1 cut(s) 519
BseDI CCNNGG 5 cut(s) 22, 35, 77, 117, 518
BseGI GGATG 1 cut(s) 142
BseLI CCNNNNNNNGG 2 cut(s) 15, 288
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 410
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 1 cut(s) 358
Bsh1236I CGCG 1 cut(s) 442
BshFI GGCC 3 cut(s) 8, 328, 390
BsiHKAI GWGCWC 2 cut(s) 65, 106
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 2 cut(s) 15, 288
BsmFI GGGAC 1 cut(s) 304
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 3 cut(s) 8, 328, 390
Bsp1286I GDGCHC 3 cut(s) 21, 65, 106
Bsp143I GATC 4 cut(s) 187, 271, 338, 528
BspACI CCGC 3 cut(s) 140, 185, 442
BspANI GGCC 3 cut(s) 8, 328, 390
BspCNI CTCAG 1 cut(s) 315
BspFNI CGCG 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 20, 189
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 2 cut(s) 182, 195
BsrI ACTGG 1 cut(s) 410
BssECI CCNNGG 5 cut(s) 22, 35, 77, 117, 518
BssMI GATC 4 cut(s) 187, 271, 338, 528
BssT1I CCWWGG 3 cut(s) 22, 35, 77
Bst2UI CCWGG 1 cut(s) 519
Bst6I CTCTTC 2 cut(s) 360, 414
BstC8I GCNNGC 3 cut(s) 183, 240, 489
BstDEI CTNAG 2 cut(s) 323, 335
BstF5I GGATG 1 cut(s) 142
BstFNI CGCG 1 cut(s) 442
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 4 cut(s) 190, 274, 341, 531
BstMBI GATC 4 cut(s) 187, 271, 338, 528
BstMWI GCNNNNNNNGC 2 cut(s) 158, 244
BstNI CCWGG 1 cut(s) 519
BstNSI RCATGY 1 cut(s) 48
BstSCI CCNGG 1 cut(s) 517
BstSFI CTRYAG 1 cut(s) 159
BstUI CGCG 1 cut(s) 442
BstV1I GCAGC 1 cut(s) 358
BstX2I RGATCY 2 cut(s) 187, 338
BstYI RGATCY 2 cut(s) 187, 338
Bsu36I CCTNAGG 1 cut(s) 323
BsuRI GGCC 3 cut(s) 8, 328, 390
BtsCI GGATG 1 cut(s) 142
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 426
Cac8I GCNNGC 3 cut(s) 183, 240, 489
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 74, 327, 388
CseI GACGC 1 cut(s) 426
Csp6I GTAC 1 cut(s) 82
CspCI CAANNNNNGTGG 2 cut(s) 366, 401
CviAII CATG 1 cut(s) 45
CviJI RGCY 8 cut(s) 8, 19, 242, 247, 328, 371, 390, 491
CviKI_1 RGCY 8 cut(s) 8, 19, 242, 247, 328, 371, 390, 491
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 2 cut(s) 323, 335
DpnI GATC 4 cut(s) 189, 273, 340, 530
DpnII GATC 4 cut(s) 187, 271, 338, 528
Eam1104I CTCTTC 2 cut(s) 360, 414
EarI CTCTTC 2 cut(s) 360, 414
EciI GGCGGA 1 cut(s) 200
Eco130I CCWWGG 3 cut(s) 22, 35, 77
Eco24I GRGCYC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 381
Eco81I CCTNAGG 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 517
EcoT14I CCWWGG 3 cut(s) 22, 35, 77
EcoT38I GRGCYC 1 cut(s) 21
ErhI CCWWGG 3 cut(s) 22, 35, 77
FaeI CATG 1 cut(s) 48
FaiI YATR 4 cut(s) 46, 93, 179, 398
FaqI GGGAC 1 cut(s) 304
FatI CATG 1 cut(s) 44
FauI CCCGC 1 cut(s) 435
Fnu4HI GCNGC 1 cut(s) 372
FokI GGATG 1 cut(s) 149
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 1 cut(s) 372
GlaI GCGC 1 cut(s) 153
GluI GCNGC 1 cut(s) 372
HaeIII GGCC 3 cut(s) 8, 328, 390
HgaI GACGC 1 cut(s) 426
HhaI GCGC 1 cut(s) 154
Hin1II CATG 1 cut(s) 48
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HinfI GANTC 2 cut(s) 221, 354
HphI GGTGA 1 cut(s) 457
Hpy166II GTNNAC 1 cut(s) 42
Hpy188I TCNGA 3 cut(s) 88, 283, 298
Hpy188III TCNNGA 3 cut(s) 203, 212, 454
Hpy8I GTNNAC 1 cut(s) 42
Hpy99I CGWCG 1 cut(s) 442
HpyAV CCTTC 1 cut(s) 471
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 1 cut(s) 161
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 244
HpyF3I CTNAG 2 cut(s) 323, 335
HpySE526I ACGT 1 cut(s) 534
Hsp92II CATG 1 cut(s) 48
HspAI GCGC 1 cut(s) 152
Kzo9I GATC 4 cut(s) 187, 271, 338, 528
LmnI GCTCC 1 cut(s) 24
LpnPI CCDG 8 cut(s) 167, 171, 299, 335, 423, 447, 504, 531
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 1 cut(s) 127
MaeII ACGT 1 cut(s) 534
MalI GATC 4 cut(s) 189, 273, 340, 530
MboI GATC 4 cut(s) 187, 271, 338, 528
MboII GAAGA 6 cut(s) 179, 244, 374, 377, 431, 498
MflI RGATCY 2 cut(s) 187, 338
MhlI GDGCHC 3 cut(s) 21, 65, 106
MluCI AATT 3 cut(s) 69, 276, 461
MlyI GAGTC 1 cut(s) 363
MmeI TCCRAC 1 cut(s) 306
MnlI CCTC 9 cut(s) 112, 127, 167, 269, 282, 318, 361, 401, 415
MseI TTAA 3 cut(s) 263, 312, 498
MslI CAYNNNNRTG 1 cut(s) 297
MspR9I CCNGG 1 cut(s) 519
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 1 cut(s) 519
MvnI CGCG 1 cut(s) 442
MwoI GCNNNNNNNGC 2 cut(s) 158, 244
NdeII GATC 4 cut(s) 187, 271, 338, 528
NlaIII CATG 1 cut(s) 48
NlaIV GGNNCC 2 cut(s) 20, 189
NmeAIII GCCGAG 1 cut(s) 268
NspI RCATGY 1 cut(s) 48
PctI GAATGC 1 cut(s) 292
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 1 cut(s) 373
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
PshBI ATTAAT 1 cut(s) 498
PsiI TTATAA 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 517
PspGI CCWGG 1 cut(s) 517
PspN4I GGNNCC 2 cut(s) 20, 189
PspPI GGNCC 3 cut(s) 74, 327, 388
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 2 cut(s) 187, 338
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
RseI CAYNNNNRTG 1 cut(s) 297
SaqAI TTAA 3 cut(s) 263, 312, 498
SatI GCNGC 1 cut(s) 372
Sau3AI GATC 4 cut(s) 187, 271, 338, 528
Sau96I GGNCC 3 cut(s) 74, 327, 388
SchI GAGTC 1 cut(s) 363
ScrFI CCNGG 1 cut(s) 519
SduI GDGCHC 3 cut(s) 21, 65, 106
SetI ASST 4 cut(s) 83, 178, 493, 537
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 159
SinI GGWCC 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 297
Sse9I AATT 3 cut(s) 69, 276, 461
SsiI CCGC 3 cut(s) 140, 185, 442
StyD4I CCNGG 1 cut(s) 517
StyI CCWWGG 3 cut(s) 22, 35, 77
TaiI ACGT 1 cut(s) 537
TaqI TCGA 2 cut(s) 274, 455
TaqII GACCGA 1 cut(s) 131
TasI AATT 3 cut(s) 69, 276, 461
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 3 cut(s) 263, 312, 498
Tru9I TTAA 3 cut(s) 263, 312, 498
TscAI CASTG 1 cut(s) 433
TseI GCWGC 1 cut(s) 371
TspDTI ATGAA 1 cut(s) 180
TspRI CASTG 1 cut(s) 433
VpaK11BI GGWCC 1 cut(s) 74
VspI ATTAAT 1 cut(s) 498
XapI RAATTY 2 cut(s) 276, 461
XceI RCATGY 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.