RLG00000006547

Coiled-coil domain-containing protein 130-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0006850
Physical Location & Seq
Forward (+)
8305522 .. 8308046
2525 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006547

Sequence Viewer

Length: 933 bp
ATGTCTTCACTTGCAGCTGCTAGAGCCGACAACTTCTATTATCCTCCGGAATGGACCCCGAATCAGGGTTCTTTGAACAAGTTTCATGGTCAGCATGCTCTGAGGGAGCGAGCCCGGAAACTGGATCAGGGAATTTTGATCATAAGGTTTGAGATGCCTTACAATATATGGTGCGGTGGGTGCAATTCTATGATTGCAAAGGGTGTTCGGTTCAATGCTGAAAAAAAGCAAGTGGGAAACTATTATTCAACAAAAATATGGAGCTTTTCCATGAAGGCTGCATGCTGCAAACAGGAGATTGTTATTCAGACAGATCCGAAGAATTGTCAGTATGTGATTATTAGCGGGGCTCAACAAAAGACTGAGGATTTTGACATTGAGGATGCAGAAACCTTTGCACTTCCTGCAGAAGAAGAAAAAGGTATGCTCGCAGATCCGTTTTACCGTCTTGAACATCAAGAAGAGGATTTGCAAAAGAAGAAAGAAGCTGAGCCAGTGCTTGTCCGCTTGCAGAGAATTTCTGACGACAGGCATTCAGATGATTATGCCCTTAACAAGGCTCTCAGGGCCAAACTTAGAGCTCAAAAGAAAAGAGTTGCTGAAGAAGAGGCTGCTTCAAGGAGAATGGGCCTCCATATACGACTGCTTCCAGCAGCAGAAGAGGATACTGCCACTGCATCACGGATGAAATTCTCTTCCAAGTTTGACAGAAATAGGAAGGATAAGCGAGCTTTGATTAATGCTTCTTCAATCTTTCCTGGGTCATCTGGGACTTCTAAGAAAGCCTTGGAACTTGAATCTAAGAGAAGGAAAATAAGTGCTGCAGCAGCATCTAGCTTACTTGCTGGGCGAGTCAAGCCTTTGTCGTGGTCTCAGGATTCTGTTTCTGCAAGCAGGCACAATGGCTCTGCAGTCAAAGTGAGACGCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

311

Amino Acids

35.02

Weight (kDa)

9.58

Isoelectric Point (pI)

58.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 13 - 278 6.3e-62 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 3 cut(s) 174, 345, 505
AclWI GGATC 3 cut(s) 132, 308, 428
AcsI RAATTY 3 cut(s) 132, 516, 689
AcuI CTGAAG 1 cut(s) 621
AfiI CCNNNNNNNGG 4 cut(s) 64, 65, 121, 556
AgsI TTSAA 7 cut(s) 76, 214, 249, 452, 618, 750, 797
AjnI CCWGG 1 cut(s) 757
AluBI AGCT 6 cut(s) 17, 264, 488, 581, 731, 837
AluI AGCT 6 cut(s) 17, 264, 488, 581, 731, 837
Alw21I GWGCWC 1 cut(s) 583
Alw26I GTCTC 2 cut(s) 876, 916
AlwI GGATC 3 cut(s) 132, 308, 428
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 2 cut(s) 567, 628
ApeKI GCWGC 9 cut(s) 14, 17, 278, 285, 611, 653, 821, 824, 827
ApoI RAATTY 3 cut(s) 132, 516, 689
AseI ATTAAT 1 cut(s) 738
AspS9I GGNCC 3 cut(s) 54, 567, 628
AsuC2I CCSGG 1 cut(s) 115
AvaII GGWCC 1 cut(s) 54
BanII GRGCYC 3 cut(s) 115, 352, 583
Bbv12I GWGCWC 1 cut(s) 583
BbvI GCAGC 9 cut(s) 4, 26, 265, 272, 598, 665, 808, 836, 839
BciT130I CCWGG 1 cut(s) 759
BciVI GTATCC 1 cut(s) 658
BclI TGATCA 1 cut(s) 138
BcnI CCSGG 1 cut(s) 115
BcoDI GTCTC 2 cut(s) 876, 916
BfaI CTAG 3 cut(s) 21, 834, 931
BfmI CTRYAG 3 cut(s) 405, 822, 909
BfuI GTATCC 1 cut(s) 658
BisI GCNGC 9 cut(s) 15, 18, 279, 286, 612, 654, 822, 825, 828
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 9 cut(s) 16, 19, 280, 287, 613, 655, 823, 826, 829
Bme1390I CCNGG 2 cut(s) 115, 759
Bme18I GGWCC 1 cut(s) 54
BmgT120I GGNCC 3 cut(s) 54, 567, 628
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 2 cut(s) 115, 759
BmsI GCATC 4 cut(s) 144, 373, 686, 839
Bpu1102I GCTNAGC 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 115
BsaI GGTCTC 1 cut(s) 876
BsaJI CCNNGG 2 cut(s) 758, 786
BsaWI WCCGGW 1 cut(s) 46
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 65, 121, 556
Bse1I ACTGG 2 cut(s) 126, 494
BseAI TCCGGA 1 cut(s) 46
BseBI CCWGG 1 cut(s) 759
BseDI CCNNGG 2 cut(s) 758, 786
BseGI GGATG 2 cut(s) 388, 690
BseLI CCNNNNNNNGG 4 cut(s) 64, 65, 121, 556
BseMII CTCAG 5 cut(s) 92, 354, 480, 577, 887
BseNI ACTGG 2 cut(s) 126, 494
BseXI GCAGC 9 cut(s) 4, 26, 265, 272, 598, 665, 808, 836, 839
BseYI CCCAGC 1 cut(s) 845
BshFI GGCC 2 cut(s) 569, 630
BsiHKAI GWGCWC 1 cut(s) 583
BsiSI CCGG 2 cut(s) 47, 115
BslFI GGGAC 1 cut(s) 784
BslI CCNNNNNNNGG 4 cut(s) 64, 65, 121, 556
BsmAI GTCTC 2 cut(s) 876, 916
BsmBI CGTCTC 1 cut(s) 916
BsmFI GGGAC 1 cut(s) 784
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 2 cut(s) 569, 630
Bso31I GGTCTC 1 cut(s) 876
Bsp1286I GDGCHC 3 cut(s) 115, 352, 583
Bsp13I TCCGGA 1 cut(s) 46
Bsp143I GATC 4 cut(s) 124, 138, 313, 433
Bsp1720I GCTNAGC 1 cut(s) 489
BspACI CCGC 3 cut(s) 174, 345, 505
BspANI GGCC 2 cut(s) 569, 630
BspCNI CTCAG 5 cut(s) 93, 355, 481, 576, 886
BspEI TCCGGA 1 cut(s) 46
BspLI GGNNCC 1 cut(s) 56
BspMAI CTGCAG 3 cut(s) 409, 826, 913
BspPI GGATC 3 cut(s) 132, 308, 428
BspTNI GGTCTC 1 cut(s) 876
BsrI ACTGG 2 cut(s) 126, 494
BssECI CCNNGG 2 cut(s) 758, 786
BssMI GATC 4 cut(s) 124, 138, 313, 433
BssT1I CCWWGG 1 cut(s) 786
Bst2UI CCWGG 1 cut(s) 759
Bst4CI ACNGT 1 cut(s) 446
Bst6I CTCTTC 4 cut(s) 456, 600, 654, 700
BstAPI GCANNNNNTGC 1 cut(s) 404
BstC8I GCNNGC 8 cut(s) 96, 111, 283, 429, 509, 729, 892, 896
BstDEI CTNAG 8 cut(s) 101, 363, 489, 563, 575, 777, 801, 873
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 2 cut(s) 388, 690
BstKTI GATC 4 cut(s) 127, 141, 316, 436
BstMAI GTCTC 2 cut(s) 876, 916
BstMBI GATC 4 cut(s) 124, 138, 313, 433
BstMWI GCNNNNNNNGC 6 cut(s) 23, 180, 404, 566, 827, 856
BstNI CCWGG 1 cut(s) 759
BstNSI RCATGY 2 cut(s) 98, 285
BstSCI CCNGG 2 cut(s) 113, 757
BstSFI CTRYAG 3 cut(s) 405, 822, 909
BstV1I GCAGC 9 cut(s) 4, 26, 265, 272, 598, 665, 808, 836, 839
BstX2I RGATCY 2 cut(s) 313, 433
BstYI RGATCY 2 cut(s) 313, 433
BsuI GTATCC 1 cut(s) 658
BsuRI GGCC 2 cut(s) 569, 630
BtsCI GGATG 2 cut(s) 388, 690
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 2 cut(s) 501, 672
Cac8I GCNNGC 8 cut(s) 96, 111, 283, 429, 509, 729, 892, 896
Cfr13I GGNCC 3 cut(s) 54, 567, 628
CviAII CATG 4 cut(s) 86, 95, 271, 282
DdeI CTNAG 8 cut(s) 101, 363, 489, 563, 575, 777, 801, 873
DpnI GATC 4 cut(s) 126, 140, 315, 435
DpnII GATC 4 cut(s) 124, 138, 313, 433
Eam1104I CTCTTC 4 cut(s) 456, 600, 654, 700
EarI CTCTTC 4 cut(s) 456, 600, 654, 700
Ecl136II GAGCTC 1 cut(s) 581
Eco130I CCWWGG 1 cut(s) 786
Eco24I GRGCYC 3 cut(s) 115, 352, 583
Eco31I GGTCTC 1 cut(s) 876
Eco47I GGWCC 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 581
Eco57I CTGAAG 1 cut(s) 621
EcoICRI GAGCTC 1 cut(s) 581
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoRII CCWGG 1 cut(s) 757
EcoT14I CCWWGG 1 cut(s) 786
EcoT38I GRGCYC 3 cut(s) 115, 352, 583
ErhI CCWWGG 1 cut(s) 786
Esp3I CGTCTC 1 cut(s) 916
FaeI CATG 4 cut(s) 89, 98, 274, 285
FalI AAGNNNNNCTT 2 cut(s) 770, 802
FaqI GGGAC 1 cut(s) 784
FatI CATG 4 cut(s) 85, 94, 270, 281
FauI CCCGC 1 cut(s) 338
FbaI TGATCA 1 cut(s) 138
Fnu4HI GCNGC 9 cut(s) 15, 18, 279, 286, 612, 654, 822, 825, 828
FokI GGATG 2 cut(s) 395, 697
FriOI GRGCYC 3 cut(s) 115, 352, 583
Fsp4HI GCNGC 9 cut(s) 15, 18, 279, 286, 612, 654, 822, 825, 828
FspBI CTAG 3 cut(s) 21, 834, 931
GluI GCNGC 9 cut(s) 15, 18, 279, 286, 612, 654, 822, 825, 828
GsaI CCCAGC 1 cut(s) 849
HaeIII GGCC 2 cut(s) 569, 630
HapII CCGG 2 cut(s) 47, 115
Hin1II CATG 4 cut(s) 89, 98, 274, 285
HinfI GANTC 4 cut(s) 61, 797, 852, 878
HpaII CCGG 2 cut(s) 47, 115
Hpy188I TCNGA 5 cut(s) 102, 309, 318, 523, 538
Hpy188III TCNNGA 4 cut(s) 47, 449, 458, 875
HpyAV CCTTC 3 cut(s) 268, 712, 801
HpyCH4III ACNGT 1 cut(s) 446
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 180, 404, 566, 827, 856
HpyF3I CTNAG 8 cut(s) 101, 363, 489, 563, 575, 777, 801, 873
Hsp92II CATG 4 cut(s) 89, 98, 274, 285
Kpn2I TCCGGA 1 cut(s) 46
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 4 cut(s) 124, 138, 313, 433
LmnI GCTCC 2 cut(s) 106, 261
Lsp1109I GCAGC 9 cut(s) 4, 26, 265, 272, 598, 665, 808, 836, 839
LweI GCATC 4 cut(s) 144, 373, 686, 839
MaeI CTAG 3 cut(s) 21, 834, 931
MalI GATC 4 cut(s) 126, 140, 315, 435
MboI GATC 4 cut(s) 124, 138, 313, 433
MflI RGATCY 2 cut(s) 313, 433
MhlI GDGCHC 3 cut(s) 115, 352, 583
MluCI AATT 5 cut(s) 132, 184, 322, 516, 689
MlyI GAGTC 1 cut(s) 861
MnlI CCTC 8 cut(s) 54, 96, 358, 373, 457, 601, 641, 655
MroI TCCGGA 1 cut(s) 46
MseI TTAA 2 cut(s) 552, 738
MslI CAYNNNNRTG 1 cut(s) 537
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 2 cut(s) 47, 115
MspR9I CCNGG 2 cut(s) 115, 759
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 1 cut(s) 759
MwoI GCNNNNNNNGC 6 cut(s) 23, 180, 404, 566, 827, 856
NciI CCSGG 1 cut(s) 115
NdeII GATC 4 cut(s) 124, 138, 313, 433
NlaIII CATG 4 cut(s) 89, 98, 274, 285
NlaIV GGNNCC 1 cut(s) 56
NspI RCATGY 2 cut(s) 98, 285
PaeI GCATGC 2 cut(s) 98, 285
PctI GAATGC 1 cut(s) 532
PfeI GAWTC 3 cut(s) 61, 797, 878
PkrI GCNGC 9 cut(s) 16, 19, 280, 287, 613, 655, 823, 826, 829
PleI GAGTC 1 cut(s) 860
PpsI GAGTC 1 cut(s) 860
PshBI ATTAAT 1 cut(s) 738
Psp124BI GAGCTC 1 cut(s) 583
Psp6I CCWGG 1 cut(s) 757
PspFI CCCAGC 1 cut(s) 845
PspGI CCWGG 1 cut(s) 757
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 3 cut(s) 54, 567, 628
PstI CTGCAG 3 cut(s) 409, 826, 913
PsuI RGATCY 2 cut(s) 313, 433
PvuII CAGCTG 1 cut(s) 17
RseI CAYNNNNRTG 1 cut(s) 537
SacI GAGCTC 1 cut(s) 583
SaqAI TTAA 2 cut(s) 552, 738
SatI GCNGC 9 cut(s) 15, 18, 279, 286, 612, 654, 822, 825, 828
Sau3AI GATC 4 cut(s) 124, 138, 313, 433
Sau96I GGNCC 3 cut(s) 54, 567, 628
SchI GAGTC 1 cut(s) 861
ScrFI CCNGG 2 cut(s) 115, 759
SduI GDGCHC 3 cut(s) 115, 352, 583
SetI ASST 9 cut(s) 19, 149, 266, 395, 424, 490, 583, 733, 839
SfaNI GCATC 4 cut(s) 144, 373, 686, 839
SfcI CTRYAG 3 cut(s) 405, 822, 909
SinI GGWCC 1 cut(s) 54
SmiMI CAYNNNNRTG 1 cut(s) 537
SphI GCATGC 2 cut(s) 98, 285
Sse9I AATT 5 cut(s) 132, 184, 322, 516, 689
SsiI CCGC 3 cut(s) 174, 345, 505
SspMI CTAG 3 cut(s) 21, 834, 931
SstI GAGCTC 1 cut(s) 583
StyD4I CCNGG 2 cut(s) 113, 757
StyI CCWWGG 1 cut(s) 786
TaaI ACNGT 1 cut(s) 446
TasI AATT 5 cut(s) 132, 184, 322, 516, 689
TfiI GAWTC 3 cut(s) 61, 797, 878
Tru1I TTAA 2 cut(s) 552, 738
Tru9I TTAA 2 cut(s) 552, 738
TscAI CASTG 2 cut(s) 501, 679
TseI GCWGC 9 cut(s) 14, 17, 278, 285, 611, 653, 821, 824, 827
TspDTI ATGAA 3 cut(s) 74, 287, 701
TspGWI ACGGA 2 cut(s) 426, 697
TspRI CASTG 2 cut(s) 501, 679
VpaK11BI GGWCC 1 cut(s) 54
VspI ATTAAT 1 cut(s) 738
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 3 cut(s) 132, 516, 689
XceI RCATGY 2 cut(s) 98, 285
XspI CTAG 3 cut(s) 21, 834, 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.