MD16G1049100.v1.1

Coiled-coil domain-containing protein 130-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006850
Physical Location & Seq
Reverse (-)
3458358 .. 3462099
3742 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1049100.v1.1.491

Sequence Viewer

Length: 942 bp
ATGTCTACACTTGCAGCTGCTAGGGCAGACAACTTTTATTATCCCCCAGAATGGTCGCCAAAGAAGGGTTCCTTGAACAAGTTCAATGGTCAACATGCTCTGAGGGAGAGAGCAAGGAAATTGGATCAGGGGATTTTGATTATAAGGTTCGAGATGCCTTATCATATATGGTGCGGTGGTTGCAATTCTATGATTGCAAAGGGTGTTCGTTTTAATGCAGAAAAAAAGCAAGTTGGAAATTACTATTCTACAAAGATATGGAGCTTTACCATGAAGTCTCCATGCTGCAAACAAGAGATTGTTATTCAGACAGATCCAAAGAATTGCAAATATGTGATTATTAGTGGGGCCACGCAGAAGAATGAGGAATTCGACATTGAGGATGCGGAAACCTTTGAACTTCCTGCTGATGAAGAAAGAGGAAAGCTTGCAGATCCATTTTATCGACTTGAACACCAAGAAGAGGATTTGCAAAAGAAGAAAGAAGCTGAGCCAGTACTTGTTCGCCTACAGCGAATTTCTGATGACAGGCATTTAGATGACTACTCCCTCAACAAGGCCCTCAGAGCCAAACTTAGATCTCAAAAGAAAAGAGTTGCGGAAGAAGAGGCTACTTCAAGGAAGATGGGTCTCGGTATACGACTGCTTCCAGTGGCAGAGGAGGATGCTGCTGCTGCGTCACTTGTGAAGTTCGTTTCCAAGTTTGAAAAGAATCGGAAGAATAAGCAAGCACTAATCAACGCTGCTTCAATTTTTCCTGGCTCATCTGGGAGTTCTATGTCGAATAAGAAGAGTTTGGAACTACAATCTAAGAGAAGAAGAATAAGTGCTGGTGCTGCGTCCAACTTACTTACAGGGGGGTTCAAGCCATCATCGTGGTCTCAGAATTCGGTTTCTTCAGGCAAGCAAAATGGAACGTCAGTCAAAGTGAGACGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

314

Amino Acids

35.32

Weight (kDa)

9.66

Isoelectric Point (pI)

59.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 13 - 272 9.8e-63 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 143
AccI GTMKAC 2 cut(s) 5, 637
AciI CCGC 3 cut(s) 174, 386, 599
AclWI GGATC 3 cut(s) 132, 308, 428
AcsI RAATTY 3 cut(s) 368, 516, 886
AcuI CTGAAG 1 cut(s) 882
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 4 cut(s) 51, 65, 463, 556
AgsI TTSAA 8 cut(s) 76, 85, 398, 452, 618, 707, 750, 865
AjnI CCWGG 1 cut(s) 757
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
AluBI AGCT 4 cut(s) 17, 264, 427, 488
AluI AGCT 4 cut(s) 17, 264, 427, 488
Alw26I GTCTC 4 cut(s) 282, 635, 885, 925
AlwI GGATC 3 cut(s) 132, 308, 428
AoxI GGCC 2 cut(s) 348, 558
ApeKI GCWGC 8 cut(s) 14, 17, 285, 668, 671, 674, 743, 836
ApoI RAATTY 3 cut(s) 368, 516, 886
Asp700I GAANNNNTTC 1 cut(s) 80
AspS9I GGNCC 2 cut(s) 348, 559
BbvI GCAGC 8 cut(s) 4, 26, 272, 655, 658, 661, 730, 823
BccI CCATC 2 cut(s) 619, 877
BciT130I CCWGG 1 cut(s) 759
BcoDI GTCTC 4 cut(s) 282, 635, 885, 925
BfaI CTAG 2 cut(s) 21, 940
BfmI CTRYAG 1 cut(s) 509
BglII AGATCT 1 cut(s) 578
BisI GCNGC 8 cut(s) 15, 18, 286, 669, 672, 675, 744, 837
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 8 cut(s) 16, 19, 287, 670, 673, 676, 745, 838
BmcAI AGTACT 1 cut(s) 498
Bme1390I CCNGG 1 cut(s) 759
BmgT120I GGNCC 2 cut(s) 348, 559
BmiI GGNNCC 2 cut(s) 70, 349
BmrFI CCNGG 1 cut(s) 759
BmsI GCATC 3 cut(s) 144, 373, 655
Bpu1102I GCTNAGC 1 cut(s) 489
BsaI GGTCTC 2 cut(s) 635, 885
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 65, 463, 556
Bse1I ACTGG 2 cut(s) 494, 650
BseBI CCWGG 1 cut(s) 759
BseGI GGATG 2 cut(s) 388, 670
BseLI CCNNNNNNNGG 4 cut(s) 51, 65, 463, 556
BseMII CTCAG 4 cut(s) 92, 480, 577, 896
BseNI ACTGG 2 cut(s) 494, 650
BseRI GAGGAG 1 cut(s) 674
BseXI GCAGC 8 cut(s) 4, 26, 272, 655, 658, 661, 730, 823
BshFI GGCC 2 cut(s) 350, 560
BslI CCNNNNNNNGG 4 cut(s) 51, 65, 463, 556
BsmAI GTCTC 4 cut(s) 282, 635, 885, 925
BsmBI CGTCTC 1 cut(s) 925
BsnI GGCC 2 cut(s) 350, 560
Bso31I GGTCTC 2 cut(s) 635, 885
Bsp143I GATC 4 cut(s) 124, 313, 433, 578
Bsp1720I GCTNAGC 1 cut(s) 489
BspACI CCGC 3 cut(s) 174, 386, 599
BspANI GGCC 2 cut(s) 350, 560
BspCNI CTCAG 4 cut(s) 93, 481, 576, 895
BspLI GGNNCC 2 cut(s) 70, 349
BspPI GGATC 3 cut(s) 132, 308, 428
BspTNI GGTCTC 2 cut(s) 635, 885
BsrI ACTGG 2 cut(s) 494, 650
BssMI GATC 4 cut(s) 124, 313, 433, 578
BssNAI GTATAC 1 cut(s) 638
Bst1107I GTATAC 1 cut(s) 638
Bst2UI CCWGG 1 cut(s) 759
Bst6I CTCTTC 3 cut(s) 456, 600, 785
BstC8I GCNNGC 3 cut(s) 429, 729, 905
BstDEI CTNAG 6 cut(s) 101, 489, 563, 575, 810, 882
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 2 cut(s) 388, 670
BstKTI GATC 4 cut(s) 127, 316, 436, 581
BstMAI GTCTC 4 cut(s) 282, 635, 885, 925
BstMBI GATC 4 cut(s) 124, 313, 433, 578
BstMWI GCNNNNNNNGC 5 cut(s) 23, 180, 566, 674, 836
BstNI CCWGG 1 cut(s) 759
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 757
BstSFI CTRYAG 1 cut(s) 509
BstV1I GCAGC 8 cut(s) 4, 26, 272, 655, 658, 661, 730, 823
BstX2I RGATCY 3 cut(s) 313, 433, 578
BstXI CCANNNNNNTGG 1 cut(s) 876
BstYI RGATCY 3 cut(s) 313, 433, 578
BstZ17I GTATAC 1 cut(s) 638
BsuRI GGCC 2 cut(s) 350, 560
BtsCI GGATG 2 cut(s) 388, 670
BtsIMutI CAGTG 1 cut(s) 657
Cac8I GCNNGC 3 cut(s) 429, 729, 905
Cfr13I GGNCC 2 cut(s) 348, 559
CseI GACGC 2 cut(s) 666, 828
Csp6I GTAC 1 cut(s) 497
CviAII CATG 3 cut(s) 95, 271, 282
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 6 cut(s) 101, 489, 563, 575, 810, 882
DpnI GATC 4 cut(s) 126, 315, 435, 580
DpnII GATC 4 cut(s) 124, 313, 433, 578
Eam1104I CTCTTC 3 cut(s) 456, 600, 785
EarI CTCTTC 3 cut(s) 456, 600, 785
Eco31I GGTCTC 2 cut(s) 635, 885
Eco57I CTGAAG 1 cut(s) 882
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 559
EcoRI GAATTC 2 cut(s) 368, 886
EcoRII CCWGG 1 cut(s) 757
Esp3I CGTCTC 1 cut(s) 925
FaeI CATG 3 cut(s) 98, 274, 285
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FatI CATG 3 cut(s) 94, 270, 281
FblI GTMKAC 2 cut(s) 5, 637
Fnu4HI GCNGC 8 cut(s) 15, 18, 286, 669, 672, 675, 744, 837
FokI GGATG 2 cut(s) 395, 677
Fsp4HI GCNGC 8 cut(s) 15, 18, 286, 669, 672, 675, 744, 837
FspBI CTAG 2 cut(s) 21, 940
GluI GCNGC 8 cut(s) 15, 18, 286, 669, 672, 675, 744, 837
HaeIII GGCC 2 cut(s) 350, 560
HgaI GACGC 2 cut(s) 666, 828
Hin1II CATG 3 cut(s) 98, 274, 285
HincII GTYRAC 1 cut(s) 92
HindII GTYRAC 1 cut(s) 92
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 1 cut(s) 712
Hpy166II GTNNAC 3 cut(s) 6, 92, 638
Hpy188I TCNGA 6 cut(s) 102, 309, 523, 566, 717, 885
Hpy188III TCNNGA 1 cut(s) 151
Hpy8I GTNNAC 3 cut(s) 6, 92, 638
HpyAV CCTTC 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 917
HpyCH4V TGCA 8 cut(s) 14, 183, 197, 218, 288, 327, 431, 472
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 180, 566, 674, 836
HpyF3I CTNAG 6 cut(s) 101, 489, 563, 575, 810, 882
HpySE526I ACGT 1 cut(s) 917
Hsp92II CATG 3 cut(s) 98, 274, 285
Kzo9I GATC 4 cut(s) 124, 313, 433, 578
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 8 cut(s) 4, 26, 272, 655, 658, 661, 730, 823
LweI GCATC 3 cut(s) 144, 373, 655
MaeI CTAG 2 cut(s) 21, 940
MaeII ACGT 1 cut(s) 917
MaeIII GTNAC 1 cut(s) 678
MalI GATC 4 cut(s) 126, 315, 435, 580
MboI GATC 4 cut(s) 124, 313, 433, 578
MflI RGATCY 3 cut(s) 313, 433, 578
MluCI AATT 8 cut(s) 119, 184, 238, 322, 368, 516, 750, 886
MmeI TCCRAC 2 cut(s) 214, 867
MroXI GAANNNNTTC 1 cut(s) 80
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 2 cut(s) 537, 874
MspA1I CMGCKG 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 759
MvaI CCWGG 1 cut(s) 759
MwoI GCNNNNNNNGC 5 cut(s) 23, 180, 566, 674, 836
NdeII GATC 4 cut(s) 124, 313, 433, 578
NlaIII CATG 3 cut(s) 98, 274, 285
NlaIV GGNNCC 2 cut(s) 70, 349
NmuCI GTSAC 1 cut(s) 678
NspI RCATGY 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 1 cut(s) 712
PkrI GCNGC 8 cut(s) 16, 19, 287, 670, 673, 676, 745, 838
PsiI TTATAA 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 757
PspGI CCWGG 1 cut(s) 757
PspN4I GGNNCC 2 cut(s) 70, 349
PspPI GGNCC 2 cut(s) 348, 559
PsuI RGATCY 3 cut(s) 313, 433, 578
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
RseI CAYNNNNRTG 2 cut(s) 537, 874
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 8 cut(s) 15, 18, 286, 669, 672, 675, 744, 837
Sau3AI GATC 4 cut(s) 124, 313, 433, 578
Sau96I GGNCC 2 cut(s) 348, 559
ScaI AGTACT 1 cut(s) 498
ScrFI CCNGG 1 cut(s) 759
SetI ASST 7 cut(s) 19, 149, 266, 395, 429, 490, 920
SfaNI GCATC 3 cut(s) 144, 373, 655
SfcI CTRYAG 1 cut(s) 509
SmiMI CAYNNNNRTG 2 cut(s) 537, 874
Sse9I AATT 8 cut(s) 119, 184, 238, 322, 368, 516, 750, 886
SsiI CCGC 3 cut(s) 174, 386, 599
SspMI CTAG 2 cut(s) 21, 940
StyD4I CCNGG 1 cut(s) 757
TaiI ACGT 1 cut(s) 920
TaqI TCGA 4 cut(s) 150, 372, 445, 782
TasI AATT 8 cut(s) 119, 184, 238, 322, 368, 516, 750, 886
TatI WGTACW 1 cut(s) 496
TfiI GAWTC 1 cut(s) 712
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 1 cut(s) 657
TseFI GTSAC 1 cut(s) 678
TseI GCWGC 8 cut(s) 14, 17, 285, 668, 671, 674, 743, 836
Tsp45I GTSAC 1 cut(s) 678
TspDTI ATGAA 2 cut(s) 287, 426
TspRI CASTG 1 cut(s) 657
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 3 cut(s) 368, 516, 886
XceI RCATGY 1 cut(s) 98
XmiI GTMKAC 2 cut(s) 5, 637
XmnI GAANNNNTTC 1 cut(s) 80
XspI CTAG 2 cut(s) 21, 940
ZrmI AGTACT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.