Rh4BG354000

Coiled-coil domain-containing protein 130-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0006850
Physical Location & Seq
Reverse (-)
51429853 .. 51432982
3130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG354000.1

Sequence Viewer

Length: 780 bp
ATGCCTTACAATATATGGTGCGGTGGGTGCAATTCTATGATTGCAAAGGGTGTTCGGTTCAATGCTGAAAAAAAGCAAGTGGGAAACTATTATTCAACAAAGATATGGAGCTTTTCCATGAAGGCTGCATGCTGCAAACAGGAGATTGTTATTCAGACAGATCCGAAGAATTGTCAGTATGTGATTATTAGCGGGGCTCAACAAAAGACTGAGGATTTTGACATTGAGGATGCAGAAACCTTTGCACTTCCTGCAGAAGAAGAAAAAGGTATGCTCGCAGATCCGTTTTACCGTCTTGAACATCAAGAAGAGGATTTGCAAAAGAAGAAAGAAGCTGAGCCAGTGCTCGTCCGCTTGCAGAGAATTTCTGACGACAGGCATTCAGATGATTATGCCCTTAACAAGGCTCTCAGGGCCAAACTTAGATCTCAAAAGAAAAGAGTTGCTGAAGAAGAGGCTGCTTCAAGGAGAATGGGCCTCCATATACGACTGCTTCCAGCGGCAGAAGAGGATACTGCCACTGCATCACGGATGAAATTCTCTTCCAAGTTTGACAGAAATAGGAAGGATAAGCGAGCTTTGATTAATGCTTCTTCAATCTTTCCTGGGTCATCTGGGACTTCTAAGAAAGCCTTGGAACTTGAATCTAAGAGAAGGAAAATAAGTGCTGCAGCAGCATCTAGCTTACTTGCTGGGCGAGTCAAGCCTTCATCGTGGTCTCAGGATTCTGTTTCTTCAAGCAGGCACAATGGCTCTGCAGTCAAAGTGAGACGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

259

Amino Acids

29.16

Weight (kDa)

9.53

Isoelectric Point (pI)

68.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 1 - 238 2.3e-51 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 21, 192, 352, 500
AclWI GGATC 2 cut(s) 155, 275
AcsI RAATTY 2 cut(s) 363, 536
AcuI CTGAAG 1 cut(s) 468
AfiI CCNNNNNNNGG 1 cut(s) 403
AgsI TTSAA 7 cut(s) 61, 96, 299, 465, 597, 644, 738
AjnI CCWGG 1 cut(s) 604
AluBI AGCT 4 cut(s) 111, 335, 578, 684
AluI AGCT 4 cut(s) 111, 335, 578, 684
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 2 cut(s) 723, 763
AlwI GGATC 2 cut(s) 155, 275
AoxI GGCC 2 cut(s) 414, 475
ApeKI GCWGC 6 cut(s) 125, 132, 458, 668, 671, 674
ApoI RAATTY 2 cut(s) 363, 536
AseI ATTAAT 1 cut(s) 585
AspS9I GGNCC 2 cut(s) 414, 475
BanII GRGCYC 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 6 cut(s) 112, 119, 445, 655, 683, 686
BciT130I CCWGG 1 cut(s) 606
BciVI GTATCC 1 cut(s) 505
BcoDI GTCTC 2 cut(s) 723, 763
BfaI CTAG 2 cut(s) 681, 778
BfmI CTRYAG 3 cut(s) 252, 669, 756
BfuI GTATCC 1 cut(s) 505
BglII AGATCT 1 cut(s) 425
BisI GCNGC 7 cut(s) 126, 133, 459, 501, 669, 672, 675
BlpI GCTNAGC 1 cut(s) 336
BlsI GCNGC 7 cut(s) 127, 134, 460, 502, 670, 673, 676
Bme1390I CCNGG 1 cut(s) 606
BmgT120I GGNCC 2 cut(s) 414, 475
BmrFI CCNGG 1 cut(s) 606
BmsI GCATC 3 cut(s) 220, 533, 686
Bpu1102I GCTNAGC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 723
BsaJI CCNNGG 2 cut(s) 605, 633
Bsc4I CCNNNNNNNGG 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 341
BseBI CCWGG 1 cut(s) 606
BseDI CCNNGG 2 cut(s) 605, 633
BseGI GGATG 2 cut(s) 235, 537
BseLI CCNNNNNNNGG 1 cut(s) 403
BseMII CTCAG 4 cut(s) 201, 327, 424, 734
BseNI ACTGG 1 cut(s) 341
BseXI GCAGC 6 cut(s) 112, 119, 445, 655, 683, 686
BseYI CCCAGC 1 cut(s) 692
BshFI GGCC 2 cut(s) 416, 477
BsiHKAI GWGCWC 1 cut(s) 348
BslFI GGGAC 1 cut(s) 631
BslI CCNNNNNNNGG 1 cut(s) 403
BsmAI GTCTC 2 cut(s) 723, 763
BsmBI CGTCTC 1 cut(s) 763
BsmFI GGGAC 1 cut(s) 631
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 2 cut(s) 416, 477
Bso31I GGTCTC 1 cut(s) 723
Bsp1286I GDGCHC 2 cut(s) 199, 348
Bsp143I GATC 3 cut(s) 160, 280, 425
Bsp1720I GCTNAGC 1 cut(s) 336
BspACI CCGC 4 cut(s) 21, 192, 352, 500
BspANI GGCC 2 cut(s) 416, 477
BspCNI CTCAG 4 cut(s) 202, 328, 423, 733
BspMAI CTGCAG 3 cut(s) 256, 673, 760
BspPI GGATC 2 cut(s) 155, 275
BspTNI GGTCTC 1 cut(s) 723
BsrI ACTGG 1 cut(s) 341
BssECI CCNNGG 2 cut(s) 605, 633
BssMI GATC 3 cut(s) 160, 280, 425
BssT1I CCWWGG 1 cut(s) 633
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 1 cut(s) 293
Bst6I CTCTTC 4 cut(s) 303, 447, 501, 547
BstAPI GCANNNNNTGC 1 cut(s) 251
BstC8I GCNNGC 5 cut(s) 130, 276, 356, 576, 743
BstDEI CTNAG 7 cut(s) 210, 336, 410, 422, 624, 648, 720
BstENI CCTNNNNNAGG 1 cut(s) 401
BstF5I GGATG 2 cut(s) 235, 537
BstKTI GATC 3 cut(s) 163, 283, 428
BstMAI GTCTC 2 cut(s) 723, 763
BstMBI GATC 3 cut(s) 160, 280, 425
BstMWI GCNNNNNNNGC 5 cut(s) 27, 251, 413, 674, 703
BstNI CCWGG 1 cut(s) 606
BstNSI RCATGY 1 cut(s) 132
BstSCI CCNGG 1 cut(s) 604
BstSFI CTRYAG 3 cut(s) 252, 669, 756
BstV1I GCAGC 6 cut(s) 112, 119, 445, 655, 683, 686
BstX2I RGATCY 3 cut(s) 160, 280, 425
BstYI RGATCY 3 cut(s) 160, 280, 425
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 2 cut(s) 416, 477
BtsCI GGATG 2 cut(s) 235, 537
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 2 cut(s) 348, 519
Cac8I GCNNGC 5 cut(s) 130, 276, 356, 576, 743
Cfr13I GGNCC 2 cut(s) 414, 475
CviAII CATG 2 cut(s) 118, 129
DdeI CTNAG 7 cut(s) 210, 336, 410, 422, 624, 648, 720
DpnI GATC 3 cut(s) 162, 282, 427
DpnII GATC 3 cut(s) 160, 280, 425
Eam1104I CTCTTC 4 cut(s) 303, 447, 501, 547
EarI CTCTTC 4 cut(s) 303, 447, 501, 547
Eco130I CCWWGG 1 cut(s) 633
Eco24I GRGCYC 1 cut(s) 199
Eco31I GGTCTC 1 cut(s) 723
Eco57I CTGAAG 1 cut(s) 468
EcoNI CCTNNNNNAGG 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 604
EcoT14I CCWWGG 1 cut(s) 633
EcoT38I GRGCYC 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 633
Esp3I CGTCTC 1 cut(s) 763
FaeI CATG 2 cut(s) 121, 132
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FaqI GGGAC 1 cut(s) 631
FatI CATG 2 cut(s) 117, 128
FauI CCCGC 1 cut(s) 185
Fnu4HI GCNGC 7 cut(s) 126, 133, 459, 501, 669, 672, 675
FokI GGATG 2 cut(s) 242, 544
FriOI GRGCYC 1 cut(s) 199
Fsp4HI GCNGC 7 cut(s) 126, 133, 459, 501, 669, 672, 675
FspBI CTAG 2 cut(s) 681, 778
GluI GCNGC 7 cut(s) 126, 133, 459, 501, 669, 672, 675
GsaI CCCAGC 1 cut(s) 696
HaeIII GGCC 2 cut(s) 416, 477
Hin1II CATG 2 cut(s) 121, 132
HinfI GANTC 3 cut(s) 644, 699, 725
Hpy188I TCNGA 4 cut(s) 156, 165, 370, 385
Hpy188III TCNNGA 3 cut(s) 296, 305, 722
HpyAV CCTTC 4 cut(s) 115, 559, 648, 717
HpyCH4III ACNGT 1 cut(s) 293
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 251, 413, 674, 703
HpyF3I CTNAG 7 cut(s) 210, 336, 410, 422, 624, 648, 720
Hsp92II CATG 2 cut(s) 121, 132
Kzo9I GATC 3 cut(s) 160, 280, 425
LmnI GCTCC 1 cut(s) 108
Lsp1109I GCAGC 6 cut(s) 112, 119, 445, 655, 683, 686
LweI GCATC 3 cut(s) 220, 533, 686
MaeI CTAG 2 cut(s) 681, 778
MalI GATC 3 cut(s) 162, 282, 427
MboI GATC 3 cut(s) 160, 280, 425
MflI RGATCY 3 cut(s) 160, 280, 425
MhlI GDGCHC 2 cut(s) 199, 348
MluCI AATT 4 cut(s) 31, 169, 363, 536
MlyI GAGTC 1 cut(s) 708
MnlI CCTC 6 cut(s) 205, 220, 304, 448, 488, 502
MseI TTAA 2 cut(s) 399, 585
MslI CAYNNNNRTG 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 500
MspR9I CCNGG 1 cut(s) 606
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 1 cut(s) 606
MwoI GCNNNNNNNGC 5 cut(s) 27, 251, 413, 674, 703
NdeII GATC 3 cut(s) 160, 280, 425
NlaIII CATG 2 cut(s) 121, 132
NspI RCATGY 1 cut(s) 132
PaeI GCATGC 1 cut(s) 132
PctI GAATGC 1 cut(s) 379
PfeI GAWTC 2 cut(s) 644, 725
PkrI GCNGC 7 cut(s) 127, 134, 460, 502, 670, 673, 676
PleI GAGTC 1 cut(s) 707
PpsI GAGTC 1 cut(s) 707
PshBI ATTAAT 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 604
PspFI CCCAGC 1 cut(s) 692
PspGI CCWGG 1 cut(s) 604
PspPI GGNCC 2 cut(s) 414, 475
PstI CTGCAG 3 cut(s) 256, 673, 760
PsuI RGATCY 3 cut(s) 160, 280, 425
RseI CAYNNNNRTG 1 cut(s) 384
SaqAI TTAA 2 cut(s) 399, 585
SatI GCNGC 7 cut(s) 126, 133, 459, 501, 669, 672, 675
Sau3AI GATC 3 cut(s) 160, 280, 425
Sau96I GGNCC 2 cut(s) 414, 475
SchI GAGTC 1 cut(s) 708
ScrFI CCNGG 1 cut(s) 606
SduI GDGCHC 2 cut(s) 199, 348
SetI ASST 6 cut(s) 113, 242, 271, 337, 580, 686
SfaNI GCATC 3 cut(s) 220, 533, 686
SfcI CTRYAG 3 cut(s) 252, 669, 756
SmiMI CAYNNNNRTG 1 cut(s) 384
SphI GCATGC 1 cut(s) 132
Sse9I AATT 4 cut(s) 31, 169, 363, 536
SsiI CCGC 4 cut(s) 21, 192, 352, 500
SspMI CTAG 2 cut(s) 681, 778
StyD4I CCNGG 1 cut(s) 604
StyI CCWWGG 1 cut(s) 633
TaaI ACNGT 1 cut(s) 293
TasI AATT 4 cut(s) 31, 169, 363, 536
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 2 cut(s) 644, 725
Tru1I TTAA 2 cut(s) 399, 585
Tru9I TTAA 2 cut(s) 399, 585
TscAI CASTG 2 cut(s) 348, 526
TseI GCWGC 6 cut(s) 125, 132, 458, 668, 671, 674
TspDTI ATGAA 3 cut(s) 134, 548, 699
TspGWI ACGGA 2 cut(s) 273, 544
TspRI CASTG 2 cut(s) 348, 526
VspI ATTAAT 1 cut(s) 585
XagI CCTNNNNNAGG 1 cut(s) 401
XapI RAATTY 2 cut(s) 363, 536
XceI RCATGY 1 cut(s) 132
XspI CTAG 2 cut(s) 681, 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.