AT1G32740

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004388
Physical Location & Seq
Forward (+)
11844660 .. 11846347
1688 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32740.2

Sequence Viewer

Length: 939 bp
ATGGCAGTTCAAGCTCAACACCATTCCTCCAATCTCCTCTTCCTCAATAAAAGAAACGGGAAAGAGAAAGAACATAGTAATTTTACATTACAATCACAAGCAGCTGGAGATTTTCTTGATCAAACCAATATGTTATTCAACAATGGAAGTTCTAATCAGAGAAAAAGAAGAAGAGAAACGAACAATCATCAGTTATTACCTATGCAGTCTCATCAGTTTCCTCAAGTTATAGACTTATCTCTATTACACAACTACAATCATCCACCGTCGAATATGGTTCATACAGGACTCCGATTATTTTCCGGCGAAGATCAGGCACAAAAGATTAGTCACCTGTCTGAAGATGTTTTTGCTGCACATATCAATAGGCAAAGCGAAGAACTTGATGAGTTTCTTCATGCCCAGGCGGAGGAGCTACGGCGTACATTAGCGGAGAAGAGGAAGATGCACTATAAAGCGCTTCTTGGTGCCGTGGAAGAGTCGTTGGTTCGTAAGCTGAGGGAGAAAGAGGTAGAGATAGAGAGAGCCACGCGCCGTCACAATGAGCTGGTGGCACGTGACTCGCAGCTGAGAGCGGAGGTGCAAGTATGGCAAGAGAGAGCTAAAGCGCACGAAGACGCCGCCGCGTCGCTGCAGTCTCAGCTCCAGCAAGCCGTTAACCAATGCGCCGGTGGATGTGTATCGGCGCAGGATAGTAGAGCGGCGGAGGAAGGGCTATTATGCACCACAATCAGCGGAGTGGATGACGCCGAGTCGGTGTACGTGGATCCGGAGAGAGTAAAGCGGCCGAATTGCAAAGCTTGCCGGGAAAGAGAGGCAACGGTGGTTGTGTTGCCGTGTCGGCATCTGAGCATCTGCCCGGGATGTGACCGGACAGCTTTAGCTTGCCCGTTGTGTCTCACGTTGCGGAATTCAAGTGTTGAAGCTATCTTTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

312

Amino Acids

35.23

Weight (kDa)

6.73

Isoelectric Point (pI)

64.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 219 - 256 1.8e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 467
AccBSI CCGCTC 2 cut(s) 575, 701
AccII CGCG 2 cut(s) 532, 626
AccIII TCCGGA 1 cut(s) 769
AclWI GGATC 2 cut(s) 761, 774
AcoI YGGCCR 1 cut(s) 785
AcsI RAATTY 1 cut(s) 910
AcuI CTGAAG 1 cut(s) 360
AcvI CACGTG 1 cut(s) 557
AcyI GRCGYC 2 cut(s) 618, 747
AfaI GTAC 2 cut(s) 424, 761
AfeI AGCGCT 1 cut(s) 459
AfiI CCNNNNNNNGG 1 cut(s) 409
AgsI TTSAA 4 cut(s) 11, 139, 915, 923
AhdI GACNNNNNGTC 1 cut(s) 751
AjnI CCWGG 1 cut(s) 402
AjuI GAANNNNNNNTTGG 4 cut(s) 23, 55, 119, 151
Alw26I GTCTC 3 cut(s) 213, 642, 902
AlwI GGATC 2 cut(s) 761, 774
Ama87I CYCGRG 1 cut(s) 859
Aor13HI TCCGGA 1 cut(s) 769
Aor51HI AGCGCT 1 cut(s) 459
AoxI GGCC 1 cut(s) 785
ApeKI GCWGC 4 cut(s) 101, 353, 565, 631
ApoI RAATTY 1 cut(s) 910
ArsI GACNNNNNNTTYG 2 cut(s) 313, 345
AspLEI GCGC 5 cut(s) 460, 534, 610, 668, 688
AsuC2I CCSGG 3 cut(s) 806, 860, 861
AsuHPI GGTGA 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 859
BamHI GGATCC 1 cut(s) 766
BanI GGYRCC 1 cut(s) 467
BbrPI CACGTG 1 cut(s) 557
BbsI GAAGAC 1 cut(s) 621
BbvCI CCTCAGC 1 cut(s) 497
BbvI GCAGC 4 cut(s) 113, 340, 577, 618
BceAI ACGGC 5 cut(s) 434, 455, 519, 638, 820
BcgI CGANNNNNNTGC 2 cut(s) 543, 577
BciT130I CCWGG 1 cut(s) 404
BclI TGATCA 1 cut(s) 118
BcnI CCSGG 3 cut(s) 806, 860, 861
BcoDI GTCTC 3 cut(s) 213, 642, 902
BfmI CTRYAG 1 cut(s) 632
BfoI RGCGCY 1 cut(s) 461
BglI GCCNNNNNGGC 1 cut(s) 841
BisI GCNGC 8 cut(s) 102, 354, 566, 621, 624, 632, 702, 785
BlsI GCNGC 8 cut(s) 103, 355, 567, 622, 625, 633, 703, 786
Bme1390I CCNGG 4 cut(s) 404, 806, 860, 861
BmeRI GACNNNNNGTC 1 cut(s) 751
BmeT110I CYCGRG 1 cut(s) 859
BmiI GGNNCC 2 cut(s) 469, 768
BmrFI CCNGG 4 cut(s) 404, 806, 860, 861
BmsI GCATC 3 cut(s) 435, 853, 861
BpiI GAAGAC 1 cut(s) 621
BpmI CTGGAG 2 cut(s) 126, 629
Bpu10I CCTNAGC 1 cut(s) 497
BpuEI CTTGAG 1 cut(s) 207
BpuMI CCSGG 3 cut(s) 806, 860, 861
BsaAI YACGTR 2 cut(s) 557, 763
BsaBI GATNNNNATC 1 cut(s) 679
BsaHI GRCGYC 2 cut(s) 618, 747
BsaJI CCNNGG 3 cut(s) 402, 471, 859
BsaWI WCCGGW 2 cut(s) 769, 870
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 1 cut(s) 409
Bse118I RCCGGY 1 cut(s) 668
Bse8I GATNNNNATC 1 cut(s) 679
BseAI TCCGGA 1 cut(s) 769
BseBI CCWGG 1 cut(s) 404
BseDI CCNNGG 3 cut(s) 402, 471, 859
BseGI GGATG 4 cut(s) 259, 680, 748, 869
BseJI GATNNNNATC 1 cut(s) 679
BseLI CCNNNNNNNGG 1 cut(s) 409
BseMII CTCAG 4 cut(s) 488, 560, 653, 839
BseRI GAGGAG 2 cut(s) 26, 425
BseX3I CGGCCG 1 cut(s) 785
BseXI GCAGC 4 cut(s) 113, 340, 577, 618
BsgI GTGCAG 1 cut(s) 339
Bsh1236I CGCG 2 cut(s) 532, 626
Bsh1285I CGRYCG 1 cut(s) 788
BshFI GGCC 1 cut(s) 787
BshNI GGYRCC 1 cut(s) 467
BsiEI CGRYCG 1 cut(s) 788
BsiHKCI CYCGRG 1 cut(s) 859
BsiSI CCGG 6 cut(s) 303, 669, 770, 805, 860, 871
BslI CCNNNNNNNGG 1 cut(s) 409
BsmAI GTCTC 3 cut(s) 213, 642, 902
BsnI GGCC 1 cut(s) 787
BsoBI CYCGRG 1 cut(s) 859
Bsp13I TCCGGA 1 cut(s) 769
Bsp143I GATC 3 cut(s) 118, 310, 766
BspANI GGCC 1 cut(s) 787
BspCNI CTCAG 4 cut(s) 489, 561, 652, 840
BspEI TCCGGA 1 cut(s) 769
BspFNI CGCG 2 cut(s) 532, 626
BspLI GGNNCC 2 cut(s) 469, 768
BspMAI CTGCAG 1 cut(s) 636
BspPI GGATC 2 cut(s) 761, 774
BspT107I GGYRCC 1 cut(s) 467
BsrBI CCGCTC 2 cut(s) 575, 701
BsrFI RCCGGY 1 cut(s) 668
BssAI RCCGGY 1 cut(s) 668
BssECI CCNNGG 3 cut(s) 402, 471, 859
BssMI GATC 3 cut(s) 118, 310, 766
BssNI GRCGYC 2 cut(s) 618, 747
Bst2UI CCWGG 1 cut(s) 404
Bst4CI ACNGT 2 cut(s) 267, 823
Bst6I CTCTTC 4 cut(s) 44, 166, 431, 471
BstACI GRCGYC 2 cut(s) 618, 747
BstAPI GCANNNNNTGC 1 cut(s) 801
BstBAI YACGTR 2 cut(s) 557, 763
BstC8I GCNNGC 3 cut(s) 651, 802, 886
BstDEI CTNAG 4 cut(s) 497, 569, 639, 848
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 4 cut(s) 259, 680, 748, 869
BstFNI CGCG 2 cut(s) 532, 626
BstH2I RGCGCY 1 cut(s) 461
BstHHI GCGC 5 cut(s) 460, 534, 610, 668, 688
BstKTI GATC 3 cut(s) 121, 313, 769
BstMAI GTCTC 3 cut(s) 213, 642, 902
BstMBI GATC 3 cut(s) 118, 310, 766
BstMCI CGRYCG 1 cut(s) 788
BstMWI GCNNNNNNNGC 5 cut(s) 11, 589, 640, 801, 841
BstNI CCWGG 1 cut(s) 404
BstSCI CCNGG 4 cut(s) 402, 804, 858, 859
BstSFI CTRYAG 1 cut(s) 632
BstUI CGCG 2 cut(s) 532, 626
BstV1I GCAGC 4 cut(s) 113, 340, 577, 618
BstV2I GAAGAC 1 cut(s) 621
BstX2I RGATCY 1 cut(s) 766
BstYI RGATCY 1 cut(s) 766
BstZI CGGCCG 1 cut(s) 785
BsuRI GGCC 1 cut(s) 787
BtgI CCRYGG 1 cut(s) 471
BtsCI GGATG 4 cut(s) 259, 680, 748, 869
Cac8I GCNNGC 3 cut(s) 651, 802, 886
CfoI GCGC 5 cut(s) 460, 534, 610, 668, 688
Cfr10I RCCGGY 1 cut(s) 668
Cfr9I CCCGGG 1 cut(s) 859
CseI GACGC 3 cut(s) 615, 626, 755
Csp6I GTAC 2 cut(s) 423, 760
CviAII CATG 1 cut(s) 398
CviQI GTAC 2 cut(s) 423, 760
DdeI CTNAG 4 cut(s) 497, 569, 639, 848
DpnI GATC 3 cut(s) 120, 312, 768
DpnII GATC 3 cut(s) 118, 310, 766
DriI GACNNNNNGTC 1 cut(s) 751
EaeI YGGCCR 1 cut(s) 785
EagI CGGCCG 1 cut(s) 785
Eam1104I CTCTTC 4 cut(s) 44, 166, 431, 471
Eam1105I GACNNNNNGTC 1 cut(s) 751
EarI CTCTTC 4 cut(s) 44, 166, 431, 471
EciI GGCGGA 2 cut(s) 422, 719
EclXI CGGCCG 1 cut(s) 785
Eco47III AGCGCT 1 cut(s) 459
Eco52I CGGCCG 1 cut(s) 785
Eco57I CTGAAG 1 cut(s) 360
Eco72I CACGTG 1 cut(s) 557
Eco88I CYCGRG 1 cut(s) 859
EcoRI GAATTC 1 cut(s) 910
EcoRII CCWGG 1 cut(s) 402
FaeI CATG 1 cut(s) 401
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FatI CATG 1 cut(s) 397
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 8 cut(s) 102, 354, 566, 621, 624, 632, 702, 785
FokI GGATG 4 cut(s) 246, 687, 755, 876
Fsp4HI GCNGC 8 cut(s) 102, 354, 566, 621, 624, 632, 702, 785
GlaI GCGC 5 cut(s) 459, 533, 609, 667, 687
GluI GCNGC 8 cut(s) 102, 354, 566, 621, 624, 632, 702, 785
GsuI CTGGAG 2 cut(s) 126, 629
HaeII RGCGCY 1 cut(s) 461
HaeIII GGCC 1 cut(s) 787
HapII CCGG 6 cut(s) 303, 669, 770, 805, 860, 871
HgaI GACGC 3 cut(s) 615, 626, 755
HhaI GCGC 5 cut(s) 460, 534, 610, 668, 688
Hin1I GRCGYC 2 cut(s) 618, 747
Hin1II CATG 1 cut(s) 401
Hin6I GCGC 5 cut(s) 458, 532, 608, 666, 686
HinP1I GCGC 5 cut(s) 458, 532, 608, 666, 686
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HindIII AAGCTT 1 cut(s) 798
HinfI GANTC 4 cut(s) 288, 479, 560, 752
HpaI GTTAAC 1 cut(s) 658
HpaII CCGG 6 cut(s) 303, 669, 770, 805, 860, 871
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 2 cut(s) 658, 760
Hpy188I TCNGA 4 cut(s) 159, 293, 340, 849
Hpy188III TCNNGA 2 cut(s) 116, 770
Hpy8I GTNNAC 2 cut(s) 658, 760
Hpy99I CGWCG 2 cut(s) 271, 631
HpyAV CCTTC 1 cut(s) 704
HpyCH4III ACNGT 2 cut(s) 267, 823
HpyCH4IV ACGT 3 cut(s) 556, 762, 902
HpyCH4V TGCA 7 cut(s) 205, 356, 448, 583, 634, 723, 795
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 589, 640, 801, 841
HpyF3I CTNAG 4 cut(s) 497, 569, 639, 848
HpySE526I ACGT 3 cut(s) 556, 762, 902
Hsp92I GRCGYC 2 cut(s) 618, 747
Hsp92II CATG 1 cut(s) 401
HspAI GCGC 5 cut(s) 458, 532, 608, 666, 686
Kpn2I TCCGGA 1 cut(s) 769
Ksp22I TGATCA 1 cut(s) 118
KspAI GTTAAC 1 cut(s) 658
Kzo9I GATC 3 cut(s) 118, 310, 766
LmnI GCTCC 2 cut(s) 412, 648
Lsp1109I GCAGC 4 cut(s) 113, 340, 577, 618
LweI GCATC 3 cut(s) 435, 853, 861
MaeII ACGT 3 cut(s) 556, 762, 902
MaeIII GTNAC 4 cut(s) 329, 536, 557, 866
MalI GATC 3 cut(s) 120, 312, 768
MbiI CCGCTC 2 cut(s) 575, 701
MboI GATC 3 cut(s) 118, 310, 766
MflI RGATCY 1 cut(s) 766
MluCI AATT 3 cut(s) 79, 790, 910
MlyI GAGTC 4 cut(s) 282, 488, 554, 761
MroI TCCGGA 1 cut(s) 769
MseI TTAA 1 cut(s) 657
MspA1I CMGCKG 3 cut(s) 104, 568, 735
MspI CCGG 6 cut(s) 303, 669, 770, 805, 860, 871
MspR9I CCNGG 4 cut(s) 404, 806, 860, 861
MvaI CCWGG 1 cut(s) 404
MvnI CGCG 2 cut(s) 532, 626
MwoI GCNNNNNNNGC 5 cut(s) 11, 589, 640, 801, 841
NciI CCSGG 3 cut(s) 806, 860, 861
NdeII GATC 3 cut(s) 118, 310, 766
NlaIII CATG 1 cut(s) 401
NlaIV GGNNCC 2 cut(s) 469, 768
NmeAIII GCCGAG 1 cut(s) 775
NmuCI GTSAC 4 cut(s) 329, 536, 557, 866
PkrI GCNGC 8 cut(s) 103, 355, 567, 622, 625, 633, 703, 786
PleI GAGTC 4 cut(s) 282, 487, 554, 760
PmaCI CACGTG 1 cut(s) 557
PmlI CACGTG 1 cut(s) 557
PpsI GAGTC 4 cut(s) 282, 487, 554, 760
Ppu21I YACGTR 2 cut(s) 557, 763
Psp6I CCWGG 1 cut(s) 402
PspCI CACGTG 1 cut(s) 557
PspGI CCWGG 1 cut(s) 402
PspN4I GGNNCC 2 cut(s) 469, 768
PstI CTGCAG 1 cut(s) 636
PsuI RGATCY 1 cut(s) 766
PvuII CAGCTG 2 cut(s) 104, 568
RsaI GTAC 2 cut(s) 424, 761
RsaNI GTAC 2 cut(s) 423, 760
SaqAI TTAA 1 cut(s) 657
SatI GCNGC 8 cut(s) 102, 354, 566, 621, 624, 632, 702, 785
Sau3AI GATC 3 cut(s) 118, 310, 766
SchI GAGTC 4 cut(s) 282, 488, 554, 761
ScrFI CCNGG 4 cut(s) 404, 806, 860, 861
SfaNI GCATC 3 cut(s) 435, 853, 861
SfcI CTRYAG 1 cut(s) 632
SgrAI CRCCGGYG 1 cut(s) 668
SmaI CCCGGG 1 cut(s) 861
SmlI CTYRAG 1 cut(s) 222
SmoI CTYRAG 1 cut(s) 222
Sse9I AATT 3 cut(s) 79, 790, 910
StyD4I CCNGG 4 cut(s) 402, 804, 858, 859
TaaI ACNGT 2 cut(s) 267, 823
TaiI ACGT 3 cut(s) 559, 765, 905
TaqI TCGA 1 cut(s) 269
TasI AATT 3 cut(s) 79, 790, 910
TauI GCSGC 4 cut(s) 623, 626, 704, 787
Tru1I TTAA 1 cut(s) 657
Tru9I TTAA 1 cut(s) 657
TseFI GTSAC 4 cut(s) 329, 536, 557, 866
TseI GCWGC 4 cut(s) 101, 353, 565, 631
Tsp45I GTSAC 4 cut(s) 329, 536, 557, 866
TspDTI ATGAA 2 cut(s) 269, 386
TspMI CCCGGG 1 cut(s) 859
XapI RAATTY 1 cut(s) 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XmaI CCCGGG 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.