Rh7CG248500

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0004388
Physical Location & Seq
Reverse (-)
23395121 .. 23396507
1387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG248500.1

Sequence Viewer

Length: 1047 bp
ATGGCAGTTCAAGCCCAGTACCCTTCCAATATTCTATTCCTAAACAGAAACGCACAAGAACCGCATGATTATTCATTACAAGCGCAACAGGGATTGTACCTTGATCAATCCCACATGCTATTCAACAATGGTGTAGGAGGGAATAGTACTAATCAGCGCAAGAGAGGAAGAGAAACTTCAGTTGGCACTGATATCACACCTTCAATGATTCCCTTCTCTTTGCAACAGTCCCAACTCATCGACCTCTCTCAGCTCCACAATCACAATCACAACCACAACCACCATCCCAATGTCGTCTCCACCGGCCTCCGATTATCTTTTGGAGACCAACAACAACAATTTCAACAGCATCATCACAATCAACAACAACAACAACAACAACAACAGCAGCAACAACAACAGGATTGTCACTCTTCTTCATCTCTGTTGTCTGTTTTGGCAGAGGACTTTGCCTCCCAAATCAAACAACAGAGGGACGAATTAGACCAGTTCCTTCAAGCCCAGGGGGAACAACTACAGCGCACATTAGCAGAGAAGCGGCAGACGCACTACCGTGCGCTACTGGGCGCAGCTGAGGAGTCTATTTCCCGTAAATTGCGAGAGAAGGAGGCAGAGGTCCAAAAGGCCACGCGCAGGAACGCCGAGTTAGAGGCACGCGCCGCCCAGCTCAGCGTTGAGGCGCAGATATGGCAGGCCAAGGCGCGTGCGCAGGAGGCCACCGCCGCGTCCCTCCAGGTCCAAGTCCAACAGGCCAAGGCCATGATGAGCGCGGGGTTCCAAGTGGCGGGTGCGCAGGATAGCAGGAGAGTCGACGATGGGCTAACATGCGCCGAAAACCAGGCGGAGGATGCCGAGTCAGGGTACATTGACCCGGAACGCGCAACCGGGTCGGGTCCGAGTTGTAAAGCGTGTCGGAAAGGAGTGGCGTCGGTGGTGCTGTTGCCGTGTCGGCACCTATGCCTCTGTACAGAGTGTGACCAAATGGTTCAGTCCTGCCCATTGTGTCTCACCTTGAGAAATTCGAGTGTGGAAGTCTTCCTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

348

Amino Acids

38.85

Weight (kDa)

6.27

Isoelectric Point (pI)

65.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 300 - 336 2.9e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 708, 792
AccB1I GGYRCC 1 cut(s) 951
AccI GTMKAC 1 cut(s) 810
AccII CGCG 6 cut(s) 631, 657, 703, 725, 770, 879
AciI CCGC 8 cut(s) 62, 538, 660, 720, 723, 770, 785, 842
AcsI RAATTY 1 cut(s) 1018
AcuI CTGAAG 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 926
AfaI GTAC 5 cut(s) 20, 98, 148, 863, 967
AfiI CCNNNNNNNGG 4 cut(s) 633, 784, 844, 858
AgsI TTSAA 5 cut(s) 11, 124, 204, 344, 497
AjnI CCWGG 3 cut(s) 501, 732, 837
AjuI GAANNNNNNNTTGG 4 cut(s) 20, 52, 165, 197
AleI CACNNNNGTG 1 cut(s) 552
AluBI AGCT 3 cut(s) 253, 572, 667
AluI AGCT 3 cut(s) 253, 572, 667
Alw26I GTCTC 3 cut(s) 301, 318, 1010
AoxI GGCC 6 cut(s) 304, 624, 693, 714, 750, 756
ApeKI GCWGC 2 cut(s) 388, 569
ApoI RAATTY 1 cut(s) 1018
AspS9I GGNCC 3 cut(s) 616, 736, 893
AsuC2I CCSGG 2 cut(s) 872, 886
AsuHPI GGTGA 1 cut(s) 1000
AvaII GGWCC 3 cut(s) 616, 736, 893
BanI GGYRCC 1 cut(s) 951
BbsI GAAGAC 1 cut(s) 1027
BbvCI CCTCAGC 1 cut(s) 573
BbvI GCAGC 2 cut(s) 400, 581
BccI CCATC 2 cut(s) 291, 809
BceAI ACGGC 1 cut(s) 928
BcgI CGANNNNNNTGC 4 cut(s) 790, 824, 870, 904
BciT130I CCWGG 3 cut(s) 503, 734, 839
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 2 cut(s) 872, 886
BcoDI GTCTC 3 cut(s) 301, 318, 1010
BfaI CTAG 1 cut(s) 1045
BfmI CTRYAG 1 cut(s) 515
BglI GCCNNNNNGGC 1 cut(s) 949
BisI GCNGC 5 cut(s) 389, 539, 570, 660, 723
BlpI GCTNAGC 1 cut(s) 668
BlsI GCNGC 5 cut(s) 390, 540, 571, 661, 724
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 5 cut(s) 503, 734, 839, 872, 886
Bme18I GGWCC 3 cut(s) 616, 736, 893
BmgT120I GGNCC 3 cut(s) 616, 736, 893
BmiI GGNNCC 3 cut(s) 776, 894, 953
BmrFI CCNGG 5 cut(s) 503, 734, 839, 872, 886
BmrI ACTGGG 2 cut(s) 10, 572
BmsI GCATC 2 cut(s) 358, 838
BmuI ACTGGG 2 cut(s) 10, 572
BpiI GAAGAC 1 cut(s) 1027
BpmI CTGGAG 1 cut(s) 716
Bpu10I CCTNAGC 1 cut(s) 573
Bpu1102I GCTNAGC 1 cut(s) 668
BpuEI CTTGAG 1 cut(s) 1033
BpuMI CCSGG 2 cut(s) 872, 886
BsaHI GRCGYC 1 cut(s) 926
BsaI GGTCTC 1 cut(s) 318
BsaJI CCNNGG 4 cut(s) 501, 502, 696, 753
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 4 cut(s) 633, 784, 844, 858
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 3 cut(s) 16, 487, 567
BseBI CCWGG 3 cut(s) 503, 734, 839
BseDI CCNNGG 4 cut(s) 501, 502, 696, 753
BseGI GGATG 2 cut(s) 283, 853
BseLI CCNNNNNNNGG 4 cut(s) 633, 784, 844, 858
BseMII CTCAG 3 cut(s) 263, 564, 682
BseNI ACTGG 3 cut(s) 16, 487, 567
BseRI GAGGAG 1 cut(s) 590
BseXI GCAGC 2 cut(s) 400, 581
BseYI CCCAGC 1 cut(s) 663
Bsh1236I CGCG 6 cut(s) 631, 657, 703, 725, 770, 879
BshFI GGCC 6 cut(s) 306, 626, 695, 716, 752, 758
BshNI GGYRCC 1 cut(s) 951
BsiSI CCGG 3 cut(s) 303, 872, 885
BslFI GGGAC 3 cut(s) 214, 488, 712
BslI CCNNNNNNNGG 4 cut(s) 633, 784, 844, 858
BsmAI GTCTC 3 cut(s) 301, 318, 1010
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 3 cut(s) 214, 488, 712
BsnI GGCC 6 cut(s) 306, 626, 695, 716, 752, 758
Bso31I GGTCTC 1 cut(s) 318
Bsp1407I TGTACA 1 cut(s) 965
Bsp143I GATC 1 cut(s) 103
Bsp1720I GCTNAGC 1 cut(s) 668
BspACI CCGC 8 cut(s) 62, 538, 660, 720, 723, 770, 785, 842
BspANI GGCC 6 cut(s) 306, 626, 695, 716, 752, 758
BspCNI CTCAG 3 cut(s) 262, 565, 681
BspFNI CGCG 6 cut(s) 631, 657, 703, 725, 770, 879
BspLI GGNNCC 3 cut(s) 776, 894, 953
BspT107I GGYRCC 1 cut(s) 951
BspTNI GGTCTC 1 cut(s) 318
BsrFI RCCGGY 1 cut(s) 302
BsrGI TGTACA 1 cut(s) 965
BsrI ACTGG 3 cut(s) 16, 487, 567
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 4 cut(s) 501, 502, 696, 753
BssMI GATC 1 cut(s) 103
BssNI GRCGYC 1 cut(s) 926
BssT1I CCWWGG 2 cut(s) 696, 753
Bst2UI CCWGG 3 cut(s) 503, 734, 839
Bst4CI ACNGT 2 cut(s) 228, 554
Bst6I CTCTTC 2 cut(s) 163, 418
BstACI GRCGYC 1 cut(s) 926
BstAUI TGTACA 1 cut(s) 965
BstC8I GCNNGC 3 cut(s) 655, 693, 705
BstDEI CTNAG 3 cut(s) 249, 573, 668
BstF5I GGATG 2 cut(s) 283, 853
BstFNI CGCG 6 cut(s) 631, 657, 703, 725, 770, 879
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 3 cut(s) 301, 318, 1010
BstMBI GATC 1 cut(s) 103
BstMWI GCNNNNNNNGC 8 cut(s) 11, 544, 659, 688, 713, 722, 848, 949
BstNI CCWGG 3 cut(s) 503, 734, 839
BstNSI RCATGY 2 cut(s) 118, 828
BstSCI CCNGG 5 cut(s) 501, 732, 837, 870, 884
BstSFI CTRYAG 1 cut(s) 515
BstUI CGCG 6 cut(s) 631, 657, 703, 725, 770, 879
BstV1I GCAGC 2 cut(s) 400, 581
BstV2I GAAGAC 1 cut(s) 1027
BsuRI GGCC 6 cut(s) 306, 626, 695, 716, 752, 758
BtsCI GGATG 2 cut(s) 283, 853
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 3 cut(s) 655, 693, 705
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 3 cut(s) 616, 736, 893
CseI GACGC 3 cut(s) 553, 714, 915
Csp6I GTAC 5 cut(s) 19, 97, 147, 862, 966
CviAII CATG 4 cut(s) 65, 115, 760, 825
CviQI GTAC 5 cut(s) 19, 97, 147, 862, 966
DdeI CTNAG 3 cut(s) 249, 573, 668
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 163, 418
EarI CTCTTC 2 cut(s) 163, 418
EciI GGCGGA 1 cut(s) 857
Eco130I CCWWGG 2 cut(s) 696, 753
Eco31I GGTCTC 1 cut(s) 318
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 3 cut(s) 616, 736, 893
Eco57I CTGAAG 1 cut(s) 162
EcoRII CCWGG 3 cut(s) 501, 732, 837
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 696, 753
ErhI CCWWGG 2 cut(s) 696, 753
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 4 cut(s) 68, 118, 763, 828
FaiI YATR 6 cut(s) 66, 116, 688, 761, 826, 958
FalI AAGNNNNNCTT 3 cut(s) 160, 192, 1023
FaqI GGGAC 3 cut(s) 214, 488, 712
FatI CATG 4 cut(s) 64, 114, 759, 824
FauI CCCGC 2 cut(s) 763, 778
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 1 cut(s) 810
Fnu4HI GCNGC 5 cut(s) 389, 539, 570, 660, 723
FokI GGATG 2 cut(s) 270, 860
Fsp4HI GCNGC 5 cut(s) 389, 539, 570, 660, 723
FspBI CTAG 1 cut(s) 1045
FspI TGCGCA 2 cut(s) 708, 792
GluI GCNGC 5 cut(s) 389, 539, 570, 660, 723
GsaI CCCAGC 1 cut(s) 667
GsuI CTGGAG 1 cut(s) 716
HaeIII GGCC 6 cut(s) 306, 626, 695, 716, 752, 758
HapII CCGG 3 cut(s) 303, 872, 885
HgaI GACGC 3 cut(s) 553, 714, 915
Hin1I GRCGYC 1 cut(s) 926
Hin1II CATG 4 cut(s) 68, 118, 763, 828
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HinfI GANTC 4 cut(s) 208, 578, 807, 854
HpaII CCGG 3 cut(s) 303, 872, 885
HphI GGTGA 1 cut(s) 1000
Hpy166II GTNNAC 1 cut(s) 811
Hpy188I TCNGA 3 cut(s) 311, 897, 915
Hpy8I GTNNAC 1 cut(s) 811
Hpy99I CGWCG 2 cut(s) 815, 931
HpyAV CCTTC 5 cut(s) 33, 210, 223, 503, 598
HpyCH4III ACNGT 2 cut(s) 228, 554
HpyCH4V TGCA 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 544, 659, 688, 713, 722, 848, 949
HpyF3I CTNAG 3 cut(s) 249, 573, 668
Hsp92I GRCGYC 1 cut(s) 926
Hsp92II CATG 4 cut(s) 68, 118, 763, 828
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 258
Lsp1109I GCAGC 2 cut(s) 400, 581
LweI GCATC 2 cut(s) 358, 838
MaeI CTAG 1 cut(s) 1045
MaeIII GTNAC 2 cut(s) 407, 974
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 4 cut(s) 180, 405, 408, 1027
MluCI AATT 4 cut(s) 338, 479, 593, 1018
MlyI GAGTC 3 cut(s) 587, 816, 863
MmeI TCCRAC 2 cut(s) 769, 893
MslI CAYNNNNRTG 2 cut(s) 288, 552
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 3 cut(s) 303, 872, 885
MspR9I CCNGG 5 cut(s) 503, 734, 839, 872, 886
MvaI CCWGG 3 cut(s) 503, 734, 839
MvnI CGCG 6 cut(s) 631, 657, 703, 725, 770, 879
MwoI GCNNNNNNNGC 8 cut(s) 11, 544, 659, 688, 713, 722, 848, 949
NciI CCSGG 2 cut(s) 872, 886
NdeII GATC 1 cut(s) 103
NlaIII CATG 4 cut(s) 68, 118, 763, 828
NlaIV GGNNCC 3 cut(s) 776, 894, 953
NmeAIII GCCGAG 2 cut(s) 667, 877
NmuCI GTSAC 2 cut(s) 407, 974
NsbI TGCGCA 2 cut(s) 708, 792
NspI RCATGY 2 cut(s) 118, 828
OliI CACNNNNGTG 1 cut(s) 552
PasI CCCWGGG 1 cut(s) 502
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 5 cut(s) 390, 540, 571, 661, 724
PleI GAGTC 3 cut(s) 586, 815, 862
PpsI GAGTC 3 cut(s) 586, 815, 862
Psp6I CCWGG 3 cut(s) 501, 732, 837
PspFI CCCAGC 1 cut(s) 663
PspGI CCWGG 3 cut(s) 501, 732, 837
PspN4I GGNNCC 3 cut(s) 776, 894, 953
PspPI GGNCC 3 cut(s) 616, 736, 893
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 5 cut(s) 20, 98, 148, 863, 967
RsaNI GTAC 5 cut(s) 19, 97, 147, 862, 966
RseI CAYNNNNRTG 2 cut(s) 288, 552
SalI GTCGAC 1 cut(s) 809
SatI GCNGC 5 cut(s) 389, 539, 570, 660, 723
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 3 cut(s) 616, 736, 893
ScaI AGTACT 1 cut(s) 148
SchI GAGTC 3 cut(s) 587, 816, 863
ScrFI CCNGG 5 cut(s) 503, 734, 839, 872, 886
SfaNI GCATC 2 cut(s) 358, 838
SfcI CTRYAG 1 cut(s) 515
SinI GGWCC 3 cut(s) 616, 736, 893
SmiMI CAYNNNNRTG 2 cut(s) 288, 552
SmlI CTYRAG 1 cut(s) 1012
SmoI CTYRAG 1 cut(s) 1012
Sse9I AATT 4 cut(s) 338, 479, 593, 1018
SsiI CCGC 8 cut(s) 62, 538, 660, 720, 723, 770, 785, 842
SspI AATATT 1 cut(s) 31
SspMI CTAG 1 cut(s) 1045
StyD4I CCNGG 5 cut(s) 501, 732, 837, 870, 884
StyI CCWWGG 2 cut(s) 696, 753
TaaI ACNGT 2 cut(s) 228, 554
TaqI TCGA 3 cut(s) 240, 810, 1022
TasI AATT 4 cut(s) 338, 479, 593, 1018
TatI WGTACW 2 cut(s) 146, 965
TauI GCSGC 3 cut(s) 541, 662, 725
TfiI GAWTC 1 cut(s) 208
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 2 cut(s) 407, 974
TseI GCWGC 2 cut(s) 388, 569
Tsp45I GTSAC 2 cut(s) 407, 974
TspDTI ATGAA 2 cut(s) 63, 408
TspRI CASTG 1 cut(s) 193
VpaK11BI GGWCC 3 cut(s) 616, 736, 893
XapI RAATTY 1 cut(s) 1018
XceI RCATGY 2 cut(s) 118, 828
XmiI GTMKAC 1 cut(s) 810
XspI CTAG 1 cut(s) 1045
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.