RLG00000003280

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004388
Physical Location & Seq
Forward (+)
46536732 .. 46538136
1405 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003280

Sequence Viewer

Length: 1062 bp
ATGGCAGTTCAAGCTCAGTACCCTTCCAATATTCTATTCCTAAACAGAAACGCACAAGAACCGCATGATTATTCATTACAAGCGCAACAAGGATTGTACCTTGATCAATCCCACATGCTATTCAACAATGGTGTAGGAGGGAATAGTACTAATCAGCGCAAGAGAGGAAGAGAAACTTCAGTTGGCACTGATATCACACCTTCAATGATTCCCTTCTCTTTGCAACAGTCCCAACCTCCTCAACTCATCGACCTCTCCCAGCTCCACAATCACAATCACAACCACAACCACCATCCCAATGTCGTCTCCACTGGCCTCCGATTATCTTTTGGAGACCAACAACAACAACAACAACAACAACATCAGCAACAATTGCAACAGCATCATCACAATCAACAACAACAGCAACAACAACAGGATTGCCACTCTTCTTCATCTCTGTTGTCTGTTTTGGCAGAGGACTTTGCCTCCCAAATCAAACAACAGAGGGACGAATTAGACCAGTTCCTTCAAGCCCAGGGGGAACAACTACAGCGCACATTAGCAGAGAAGCGGCAGAGACACTACCGTGCGCTACTCGGCGCAGCTGAGGAGTCTATTTCCCGTAAATTGCGAGAGAAGGAGGCAGAGGTCCAAAAGGCCACGCGCAGGTACGCCGAGTTAGAGGCACGCGCCTCCCAGCTCAGCATTGAGGCGCAGATATGGCAGGCCAAGGCGCGTGCGCAGGAGGCCACCGCCGCGTCCCTCCAGGTCCAAGTCCAACAGGCCAAGGCCATGATGAGCGCCGGGTTCCAAGTAGCGGGTGCGCAGGATAGCAGGAGAGCCGACGATGGGCTAACATGCGCCGAAAACCAGGCGGAGGATGCCGAGTCAGGGTACATTGATCCGGAACGCGCCACCGGGTCGGGTCCGAGTTGTAAAGCGTGTCGGAAAGGAGTGGCGTCCGTGGTGCTGTTGCCGTGTCGGCACCTATGCCTCTGTACGGAGTGTGACCAAATGGTTCAGTCCTGCCCATTGTGCCTCACCTTGAGAAATTCGAGTGTGGAAGTCTTCCTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

354

Amino Acids

39.51

Weight (kDa)

6.43

Isoelectric Point (pI)

69.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 305 - 341 2.9e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 723, 807
Acc36I ACCTGC 1 cut(s) 639
AccB1I GGYRCC 1 cut(s) 966
AccII CGCG 5 cut(s) 646, 672, 718, 740, 894
AccIII TCCGGA 1 cut(s) 886
AciI CCGC 6 cut(s) 62, 553, 735, 738, 800, 857
AclWI GGATC 1 cut(s) 878
AcsI RAATTY 1 cut(s) 1033
AcuI CTGAAG 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 941
AfaI GTAC 6 cut(s) 20, 98, 148, 653, 878, 982
AfiI CCNNNNNNNGG 6 cut(s) 648, 799, 831, 859, 873, 982
AgsI TTSAA 4 cut(s) 11, 124, 204, 512
AjnI CCWGG 3 cut(s) 516, 747, 852
AjuI GAANNNNNNNTTGG 4 cut(s) 20, 52, 165, 197
AleI CACNNNNGTG 1 cut(s) 567
AluBI AGCT 4 cut(s) 14, 262, 587, 682
AluI AGCT 4 cut(s) 14, 262, 587, 682
Alw26I GTCTC 3 cut(s) 310, 327, 553
AlwI GGATC 1 cut(s) 878
Aor13HI TCCGGA 1 cut(s) 886
AoxI GGCC 6 cut(s) 313, 639, 708, 729, 765, 771
ApeKI GCWGC 1 cut(s) 584
ApoI RAATTY 1 cut(s) 1033
AspS9I GGNCC 3 cut(s) 631, 751, 908
AsuC2I CCSGG 2 cut(s) 787, 901
AsuHPI GGTGA 1 cut(s) 1015
AvaII GGWCC 3 cut(s) 631, 751, 908
BanI GGYRCC 1 cut(s) 966
BbsI GAAGAC 1 cut(s) 1042
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 1 cut(s) 596
BccI CCATC 2 cut(s) 300, 824
BceAI ACGGC 1 cut(s) 943
BciT130I CCWGG 3 cut(s) 518, 749, 854
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 2 cut(s) 787, 901
BcoDI GTCTC 3 cut(s) 310, 327, 553
BfaI CTAG 1 cut(s) 1060
BfmI CTRYAG 1 cut(s) 530
BfoI RGCGCY 1 cut(s) 786
BfuAI ACCTGC 1 cut(s) 639
BglI GCCNNNNNGGC 1 cut(s) 964
BisI GCNGC 3 cut(s) 554, 585, 738
BlpI GCTNAGC 1 cut(s) 683
BlsI GCNGC 3 cut(s) 555, 586, 739
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 5 cut(s) 518, 749, 787, 854, 901
Bme18I GGWCC 3 cut(s) 631, 751, 908
BmgT120I GGNCC 3 cut(s) 631, 751, 908
BmiI GGNNCC 3 cut(s) 791, 909, 968
BmrFI CCNGG 5 cut(s) 518, 749, 787, 854, 901
BmsI GCATC 2 cut(s) 391, 853
BpiI GAAGAC 1 cut(s) 1042
BpmI CTGGAG 1 cut(s) 731
Bpu10I CCTNAGC 1 cut(s) 588
Bpu1102I GCTNAGC 1 cut(s) 683
BpuEI CTTGAG 1 cut(s) 1048
BpuMI CCSGG 2 cut(s) 787, 901
BsaHI GRCGYC 1 cut(s) 941
BsaI GGTCTC 1 cut(s) 327
BsaJI CCNNGG 5 cut(s) 516, 517, 711, 768, 945
BsaWI WCCGGW 1 cut(s) 886
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 6 cut(s) 648, 799, 831, 859, 873, 982
Bse1I ACTGG 2 cut(s) 316, 502
BseAI TCCGGA 1 cut(s) 886
BseBI CCWGG 3 cut(s) 518, 749, 854
BseDI CCNNGG 5 cut(s) 516, 517, 711, 768, 945
BseGI GGATG 2 cut(s) 292, 868
BseLI CCNNNNNNNGG 6 cut(s) 648, 799, 831, 859, 873, 982
BseMII CTCAG 3 cut(s) 29, 579, 697
BseNI ACTGG 2 cut(s) 316, 502
BseRI GAGGAG 2 cut(s) 228, 605
BseXI GCAGC 1 cut(s) 596
BseYI CCCAGC 2 cut(s) 258, 678
Bsh1236I CGCG 5 cut(s) 646, 672, 718, 740, 894
BshFI GGCC 6 cut(s) 315, 641, 710, 731, 767, 773
BshNI GGYRCC 1 cut(s) 966
BsiSI CCGG 3 cut(s) 786, 887, 900
BslFI GGGAC 3 cut(s) 214, 503, 727
BslI CCNNNNNNNGG 6 cut(s) 648, 799, 831, 859, 873, 982
BsmAI GTCTC 3 cut(s) 310, 327, 553
BsmBI CGTCTC 1 cut(s) 310
BsmFI GGGAC 3 cut(s) 214, 503, 727
BsnI GGCC 6 cut(s) 315, 641, 710, 731, 767, 773
Bso31I GGTCTC 1 cut(s) 327
Bsp13I TCCGGA 1 cut(s) 886
Bsp143I GATC 2 cut(s) 103, 883
Bsp1720I GCTNAGC 1 cut(s) 683
BspACI CCGC 6 cut(s) 62, 553, 735, 738, 800, 857
BspANI GGCC 6 cut(s) 315, 641, 710, 731, 767, 773
BspCNI CTCAG 3 cut(s) 28, 580, 696
BspEI TCCGGA 1 cut(s) 886
BspFNI CGCG 5 cut(s) 646, 672, 718, 740, 894
BspLI GGNNCC 3 cut(s) 791, 909, 968
BspMI ACCTGC 1 cut(s) 639
BspPI GGATC 1 cut(s) 878
BspT107I GGYRCC 1 cut(s) 966
BspTNI GGTCTC 1 cut(s) 327
BsrI ACTGG 2 cut(s) 316, 502
BssECI CCNNGG 5 cut(s) 516, 517, 711, 768, 945
BssMI GATC 2 cut(s) 103, 883
BssNI GRCGYC 1 cut(s) 941
BssT1I CCWWGG 2 cut(s) 711, 768
Bst2UI CCWGG 3 cut(s) 518, 749, 854
Bst4CI ACNGT 2 cut(s) 228, 569
Bst6I CTCTTC 2 cut(s) 163, 433
BstACI GRCGYC 1 cut(s) 941
BstAPI GCANNNNNTGC 1 cut(s) 373
BstC8I GCNNGC 3 cut(s) 670, 708, 720
BstDEI CTNAG 3 cut(s) 15, 588, 683
BstDSI CCRYGG 1 cut(s) 945
BstF5I GGATG 2 cut(s) 292, 868
BstFNI CGCG 5 cut(s) 646, 672, 718, 740, 894
BstH2I RGCGCY 1 cut(s) 786
BstKTI GATC 2 cut(s) 106, 886
BstMAI GTCTC 3 cut(s) 310, 327, 553
BstMBI GATC 2 cut(s) 103, 883
BstMWI GCNNNNNNNGC 8 cut(s) 11, 373, 703, 728, 737, 863, 964, 1017
BstNI CCWGG 3 cut(s) 518, 749, 854
BstNSI RCATGY 2 cut(s) 118, 843
BstSCI CCNGG 5 cut(s) 516, 747, 785, 852, 899
BstSFI CTRYAG 1 cut(s) 530
BstUI CGCG 5 cut(s) 646, 672, 718, 740, 894
BstV1I GCAGC 1 cut(s) 596
BstV2I GAAGAC 1 cut(s) 1042
BsuRI GGCC 6 cut(s) 315, 641, 710, 731, 767, 773
BtgI CCRYGG 1 cut(s) 945
BtsCI GGATG 2 cut(s) 292, 868
BtsIMutI CAGTG 2 cut(s) 186, 309
BveI ACCTGC 1 cut(s) 639
Cac8I GCNNGC 3 cut(s) 670, 708, 720
Cfr13I GGNCC 3 cut(s) 631, 751, 908
CseI GACGC 2 cut(s) 729, 930
Csp6I GTAC 6 cut(s) 19, 97, 147, 652, 877, 981
CviAII CATG 4 cut(s) 65, 115, 775, 840
CviQI GTAC 6 cut(s) 19, 97, 147, 652, 877, 981
DdeI CTNAG 3 cut(s) 15, 588, 683
DpnI GATC 2 cut(s) 105, 885
DpnII GATC 2 cut(s) 103, 883
Eam1104I CTCTTC 2 cut(s) 163, 433
EarI CTCTTC 2 cut(s) 163, 433
EciI GGCGGA 1 cut(s) 872
Eco130I CCWWGG 2 cut(s) 711, 768
Eco31I GGTCTC 1 cut(s) 327
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 3 cut(s) 631, 751, 908
Eco57I CTGAAG 1 cut(s) 162
EcoRII CCWGG 3 cut(s) 516, 747, 852
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 711, 768
ErhI CCWWGG 2 cut(s) 711, 768
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 4 cut(s) 68, 118, 778, 843
FaiI YATR 6 cut(s) 66, 116, 703, 776, 841, 973
FalI AAGNNNNNCTT 3 cut(s) 160, 192, 1038
FaqI GGGAC 3 cut(s) 214, 503, 727
FatI CATG 4 cut(s) 64, 114, 774, 839
FauI CCCGC 1 cut(s) 793
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 554, 585, 738
FokI GGATG 2 cut(s) 279, 875
Fsp4HI GCNGC 3 cut(s) 554, 585, 738
FspBI CTAG 1 cut(s) 1060
FspI TGCGCA 2 cut(s) 723, 807
GluI GCNGC 3 cut(s) 554, 585, 738
GsaI CCCAGC 2 cut(s) 262, 682
GsuI CTGGAG 1 cut(s) 731
HaeII RGCGCY 1 cut(s) 786
HaeIII GGCC 6 cut(s) 315, 641, 710, 731, 767, 773
HapII CCGG 3 cut(s) 786, 887, 900
HgaI GACGC 2 cut(s) 729, 930
Hin1I GRCGYC 1 cut(s) 941
Hin1II CATG 4 cut(s) 68, 118, 778, 843
HinfI GANTC 3 cut(s) 208, 593, 869
HpaII CCGG 3 cut(s) 786, 887, 900
HphI GGTGA 1 cut(s) 1015
Hpy188I TCNGA 3 cut(s) 320, 912, 930
Hpy188III TCNNGA 1 cut(s) 887
Hpy99I CGWCG 1 cut(s) 830
HpyAV CCTTC 5 cut(s) 33, 210, 223, 518, 613
HpyCH4III ACNGT 2 cut(s) 228, 569
HpyCH4V TGCA 2 cut(s) 223, 376
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 373, 703, 728, 737, 863, 964, 1017
HpyF3I CTNAG 3 cut(s) 15, 588, 683
Hsp92I GRCGYC 1 cut(s) 941
Hsp92II CATG 4 cut(s) 68, 118, 778, 843
Kpn2I TCCGGA 1 cut(s) 886
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 2 cut(s) 103, 883
LmnI GCTCC 1 cut(s) 267
Lsp1109I GCAGC 1 cut(s) 596
LweI GCATC 2 cut(s) 391, 853
MaeI CTAG 1 cut(s) 1060
MaeIII GTNAC 1 cut(s) 989
MalI GATC 2 cut(s) 105, 885
MboI GATC 2 cut(s) 103, 883
MboII GAAGA 4 cut(s) 180, 420, 423, 1042
MfeI CAATTG 1 cut(s) 371
MluCI AATT 4 cut(s) 371, 494, 608, 1033
MlyI GAGTC 2 cut(s) 602, 878
MmeI TCCRAC 2 cut(s) 784, 908
MroI TCCGGA 1 cut(s) 886
MslI CAYNNNNRTG 2 cut(s) 297, 567
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 3 cut(s) 786, 887, 900
MspR9I CCNGG 5 cut(s) 518, 749, 787, 854, 901
MunI CAATTG 1 cut(s) 371
MvaI CCWGG 3 cut(s) 518, 749, 854
MvnI CGCG 5 cut(s) 646, 672, 718, 740, 894
MwoI GCNNNNNNNGC 8 cut(s) 11, 373, 703, 728, 737, 863, 964, 1017
NciI CCSGG 2 cut(s) 787, 901
NdeII GATC 2 cut(s) 103, 883
NlaIII CATG 4 cut(s) 68, 118, 778, 843
NlaIV GGNNCC 3 cut(s) 791, 909, 968
NmeAIII GCCGAG 3 cut(s) 558, 682, 892
NmuCI GTSAC 1 cut(s) 989
NsbI TGCGCA 2 cut(s) 723, 807
NspI RCATGY 2 cut(s) 118, 843
OliI CACNNNNGTG 1 cut(s) 567
PasI CCCWGGG 1 cut(s) 517
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 3 cut(s) 555, 586, 739
PleI GAGTC 2 cut(s) 601, 877
PpsI GAGTC 2 cut(s) 601, 877
Psp6I CCWGG 3 cut(s) 516, 747, 852
PspFI CCCAGC 2 cut(s) 258, 678
PspGI CCWGG 3 cut(s) 516, 747, 852
PspN4I GGNNCC 3 cut(s) 791, 909, 968
PspPI GGNCC 3 cut(s) 631, 751, 908
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 6 cut(s) 20, 98, 148, 653, 878, 982
RsaNI GTAC 6 cut(s) 19, 97, 147, 652, 877, 981
RseI CAYNNNNRTG 2 cut(s) 297, 567
SatI GCNGC 3 cut(s) 554, 585, 738
Sau3AI GATC 2 cut(s) 103, 883
Sau96I GGNCC 3 cut(s) 631, 751, 908
ScaI AGTACT 1 cut(s) 148
SchI GAGTC 2 cut(s) 602, 878
ScrFI CCNGG 5 cut(s) 518, 749, 787, 854, 901
SfaNI GCATC 2 cut(s) 391, 853
SfcI CTRYAG 1 cut(s) 530
SinI GGWCC 3 cut(s) 631, 751, 908
SmiMI CAYNNNNRTG 2 cut(s) 297, 567
SmlI CTYRAG 1 cut(s) 1027
SmoI CTYRAG 1 cut(s) 1027
Sse9I AATT 4 cut(s) 371, 494, 608, 1033
SsiI CCGC 6 cut(s) 62, 553, 735, 738, 800, 857
SspI AATATT 1 cut(s) 31
SspMI CTAG 1 cut(s) 1060
StyD4I CCNGG 5 cut(s) 516, 747, 785, 852, 899
StyI CCWWGG 2 cut(s) 711, 768
TaaI ACNGT 2 cut(s) 228, 569
TaqI TCGA 2 cut(s) 249, 1037
TasI AATT 4 cut(s) 371, 494, 608, 1033
TatI WGTACW 1 cut(s) 146
TauI GCSGC 2 cut(s) 556, 740
TfiI GAWTC 1 cut(s) 208
TscAI CASTG 2 cut(s) 193, 316
TseFI GTSAC 1 cut(s) 989
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 1 cut(s) 989
TspDTI ATGAA 2 cut(s) 63, 423
TspGWI ACGGA 2 cut(s) 934, 998
TspRI CASTG 2 cut(s) 193, 316
VpaK11BI GGWCC 3 cut(s) 631, 751, 908
XapI RAATTY 1 cut(s) 1033
XceI RCATGY 2 cut(s) 118, 843
XspI CTAG 1 cut(s) 1060
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.