Rroxscaffold_3G00251090

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004388
Physical Location & Seq
Forward (+)
44955004 .. 44957463
2460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00251090.1

Sequence Viewer

Length: 1038 bp
ATGGCAGTTCAAGCTCAGTACCCTTCCAATATTTTATTCCTAAACAGAAACGCACAAGAACCGCATGATTATTCATTACAAGCGCAACAAGGATTGTACCTTGATCAATCCCACATGCTATTCAACAATGGTGTAGGAGGGAATACTACTAATCAGCGCAAGAGAGGAAGAGAAACTTCAGTTGGCACTGATATCACACCTTCAATGATTCCCTTCTCTTTGCAACAGTCCCAACCTTCTCAACTCATCGACCTCTCTCAGCTCCACAATCACAACCACAACCACCATCCCAATGTCGTCTCCACCGGCCTCCGATTATCTTTTGGAGACCAGCAACAACAACAACATCAGCAGCAATTGCAACAGCATCATCACAATCAACAACAACAACAGGATTGTCACTCTTCTTCGTCTCTGTTGTCTGTTTTGGCAGAGGACTTTGCCTCCCAAATCAAACAACAGAGGGACGAATTAGACCAGTTCCTTCAAGCCCAGGGGGAACAACTACAGCGCACATTAGCAGAGAAGCGGCAGACACACTACCGTGCGCTACTGGGCGCAGCTGAGGAGTCTATTTCCCGTAAATTGCGAGAGAAGGAGGCAGAGGTCCAAAAGGCCACGCGCAGGAACGCCGAGTTAGAGGCACGCGCCGCCCAGCTCAGAGTTGAGGCCCAGATATGGCAGGCCAAGGCGCGTGCGCAGGAGGCTACGGCCGCGTCCCTCCAGGTCCAAGTCCAACAGGCCAAGGCCATGATGAGCGCCGGGTTCCAAGTGGCGGGTGCGCAGGATAGCAGGAGAGTCGACGATGGGCTAACATGCGCTGAAAACCAGGCTGAGGATGCCGAGTCAGGGTACATTGACCCGGAACGCGCCACAGGGTCGGGTCCGAGTTGTAAAGCGTGTCGGAAAGGAGTGGCGTCGGTGGTGCTGTTGCCGTGTCGGCACCTATGCCTCTGTACAGAGTGTGACCAAATGGTTCAGTCCTGCCCATTGTGCCTCACCTTGAGAAATTCGAGTGTGGAAGTCTTCCTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

345

Amino Acids

38.46

Weight (kDa)

6.39

Isoelectric Point (pI)

64.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 297 - 333 2.9e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 699, 783
AccB1I GGYRCC 1 cut(s) 942
AccI GTMKAC 1 cut(s) 801
AccII CGCG 5 cut(s) 622, 648, 694, 716, 870
AciI CCGC 5 cut(s) 62, 529, 651, 714, 776
AcoI YGGCCR 1 cut(s) 711
AcsI RAATTY 1 cut(s) 1009
AcuI CTGAAG 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 917
AfaI GTAC 4 cut(s) 20, 98, 854, 958
AfiI CCNNNNNNNGG 5 cut(s) 624, 678, 775, 835, 849
AgsI TTSAA 4 cut(s) 11, 124, 204, 488
AjnI CCWGG 3 cut(s) 492, 723, 828
AjuI GAANNNNNNNTTGG 4 cut(s) 20, 52, 165, 197
AleI CACNNNNGTG 1 cut(s) 543
AluBI AGCT 4 cut(s) 14, 262, 563, 658
AluI AGCT 4 cut(s) 14, 262, 563, 658
Alw26I GTCTC 3 cut(s) 304, 321, 417
AoxI GGCC 7 cut(s) 307, 615, 669, 684, 711, 741, 747
ApeKI GCWGC 2 cut(s) 352, 560
ApoI RAATTY 1 cut(s) 1009
AspS9I GGNCC 4 cut(s) 607, 670, 727, 884
AsuC2I CCSGG 2 cut(s) 763, 863
AsuHPI GGTGA 1 cut(s) 991
AvaII GGWCC 3 cut(s) 607, 727, 884
BanI GGYRCC 1 cut(s) 942
BbsI GAAGAC 1 cut(s) 1018
BbvCI CCTCAGC 2 cut(s) 564, 834
BbvI GCAGC 2 cut(s) 364, 572
BccI CCATC 2 cut(s) 294, 800
BceAI ACGGC 2 cut(s) 726, 919
BcgI CGANNNNNNTGC 2 cut(s) 781, 815
BciT130I CCWGG 3 cut(s) 494, 725, 830
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 2 cut(s) 763, 863
BcoDI GTCTC 3 cut(s) 304, 321, 417
BfaI CTAG 1 cut(s) 1036
BfmI CTRYAG 1 cut(s) 506
BfoI RGCGCY 1 cut(s) 762
BglI GCCNNNNNGGC 1 cut(s) 940
BisI GCNGC 5 cut(s) 353, 530, 561, 651, 714
BlsI GCNGC 5 cut(s) 354, 531, 562, 652, 715
Bme1390I CCNGG 5 cut(s) 494, 725, 763, 830, 863
Bme18I GGWCC 3 cut(s) 607, 727, 884
BmgT120I GGNCC 4 cut(s) 607, 670, 727, 884
BmiI GGNNCC 3 cut(s) 767, 885, 944
BmrFI CCNGG 5 cut(s) 494, 725, 763, 830, 863
BmrI ACTGGG 1 cut(s) 563
BmsI GCATC 2 cut(s) 376, 829
BmuI ACTGGG 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 1018
BpmI CTGGAG 1 cut(s) 707
Bpu10I CCTNAGC 2 cut(s) 564, 834
BpuEI CTTGAG 1 cut(s) 1024
BpuMI CCSGG 2 cut(s) 763, 863
BsaHI GRCGYC 1 cut(s) 917
BsaI GGTCTC 1 cut(s) 321
BsaJI CCNNGG 4 cut(s) 492, 493, 687, 744
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 5 cut(s) 624, 678, 775, 835, 849
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 2 cut(s) 478, 558
BseBI CCWGG 3 cut(s) 494, 725, 830
BseDI CCNNGG 4 cut(s) 492, 493, 687, 744
BseGI GGATG 2 cut(s) 286, 844
BseLI CCNNNNNNNGG 5 cut(s) 624, 678, 775, 835, 849
BseMII CTCAG 5 cut(s) 29, 272, 555, 673, 825
BseNI ACTGG 2 cut(s) 478, 558
BseRI GAGGAG 1 cut(s) 581
BseX3I CGGCCG 1 cut(s) 711
BseXI GCAGC 2 cut(s) 364, 572
BseYI CCCAGC 1 cut(s) 654
Bsh1236I CGCG 5 cut(s) 622, 648, 694, 716, 870
Bsh1285I CGRYCG 1 cut(s) 714
BshFI GGCC 7 cut(s) 309, 617, 671, 686, 713, 743, 749
BshNI GGYRCC 1 cut(s) 942
BsiEI CGRYCG 1 cut(s) 714
BsiSI CCGG 3 cut(s) 306, 762, 863
BslFI GGGAC 3 cut(s) 214, 479, 703
BslI CCNNNNNNNGG 5 cut(s) 624, 678, 775, 835, 849
BsmAI GTCTC 3 cut(s) 304, 321, 417
BsmBI CGTCTC 2 cut(s) 304, 417
BsmFI GGGAC 3 cut(s) 214, 479, 703
BsnI GGCC 7 cut(s) 309, 617, 671, 686, 713, 743, 749
Bso31I GGTCTC 1 cut(s) 321
Bsp1407I TGTACA 1 cut(s) 956
Bsp143I GATC 1 cut(s) 103
BspACI CCGC 5 cut(s) 62, 529, 651, 714, 776
BspANI GGCC 7 cut(s) 309, 617, 671, 686, 713, 743, 749
BspCNI CTCAG 5 cut(s) 28, 271, 556, 672, 826
BspFNI CGCG 5 cut(s) 622, 648, 694, 716, 870
BspLI GGNNCC 3 cut(s) 767, 885, 944
BspT107I GGYRCC 1 cut(s) 942
BspTNI GGTCTC 1 cut(s) 321
BsrFI RCCGGY 1 cut(s) 305
BsrGI TGTACA 1 cut(s) 956
BsrI ACTGG 2 cut(s) 478, 558
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 4 cut(s) 492, 493, 687, 744
BssMI GATC 1 cut(s) 103
BssNI GRCGYC 1 cut(s) 917
BssT1I CCWWGG 2 cut(s) 687, 744
Bst2UI CCWGG 3 cut(s) 494, 725, 830
Bst4CI ACNGT 2 cut(s) 228, 545
Bst6I CTCTTC 2 cut(s) 163, 409
BstACI GRCGYC 1 cut(s) 917
BstAPI GCANNNNNTGC 1 cut(s) 358
BstAUI TGTACA 1 cut(s) 956
BstC8I GCNNGC 3 cut(s) 646, 684, 696
BstDEI CTNAG 5 cut(s) 15, 258, 564, 659, 834
BstF5I GGATG 2 cut(s) 286, 844
BstFNI CGCG 5 cut(s) 622, 648, 694, 716, 870
BstH2I RGCGCY 1 cut(s) 762
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 3 cut(s) 304, 321, 417
BstMBI GATC 1 cut(s) 103
BstMCI CGRYCG 1 cut(s) 714
BstMWI GCNNNNNNNGC 8 cut(s) 11, 358, 650, 704, 713, 839, 940, 993
BstNI CCWGG 3 cut(s) 494, 725, 830
BstNSI RCATGY 2 cut(s) 118, 819
BstSCI CCNGG 5 cut(s) 492, 723, 761, 828, 861
BstSFI CTRYAG 1 cut(s) 506
BstUI CGCG 5 cut(s) 622, 648, 694, 716, 870
BstV1I GCAGC 2 cut(s) 364, 572
BstV2I GAAGAC 1 cut(s) 1018
BstZI CGGCCG 1 cut(s) 711
BsuRI GGCC 7 cut(s) 309, 617, 671, 686, 713, 743, 749
BtsCI GGATG 2 cut(s) 286, 844
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 3 cut(s) 646, 684, 696
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 4 cut(s) 607, 670, 727, 884
CseI GACGC 2 cut(s) 705, 906
Csp6I GTAC 4 cut(s) 19, 97, 853, 957
CviAII CATG 4 cut(s) 65, 115, 751, 816
CviQI GTAC 4 cut(s) 19, 97, 853, 957
DdeI CTNAG 5 cut(s) 15, 258, 564, 659, 834
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 711
EagI CGGCCG 1 cut(s) 711
Eam1104I CTCTTC 2 cut(s) 163, 409
EarI CTCTTC 2 cut(s) 163, 409
EclXI CGGCCG 1 cut(s) 711
Eco130I CCWWGG 2 cut(s) 687, 744
Eco31I GGTCTC 1 cut(s) 321
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 3 cut(s) 607, 727, 884
Eco52I CGGCCG 1 cut(s) 711
Eco57I CTGAAG 1 cut(s) 162
EcoRII CCWGG 3 cut(s) 492, 723, 828
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 687, 744
ErhI CCWWGG 2 cut(s) 687, 744
Esp3I CGTCTC 2 cut(s) 304, 417
FaeI CATG 4 cut(s) 68, 118, 754, 819
FaiI YATR 6 cut(s) 66, 116, 679, 752, 817, 949
FalI AAGNNNNNCTT 3 cut(s) 160, 192, 1014
FaqI GGGAC 3 cut(s) 214, 479, 703
FatI CATG 4 cut(s) 64, 114, 750, 815
FauI CCCGC 1 cut(s) 769
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 1 cut(s) 801
Fnu4HI GCNGC 5 cut(s) 353, 530, 561, 651, 714
FokI GGATG 2 cut(s) 273, 851
Fsp4HI GCNGC 5 cut(s) 353, 530, 561, 651, 714
FspBI CTAG 1 cut(s) 1036
FspI TGCGCA 2 cut(s) 699, 783
GluI GCNGC 5 cut(s) 353, 530, 561, 651, 714
GsaI CCCAGC 1 cut(s) 658
GsuI CTGGAG 1 cut(s) 707
HaeII RGCGCY 1 cut(s) 762
HaeIII GGCC 7 cut(s) 309, 617, 671, 686, 713, 743, 749
HapII CCGG 3 cut(s) 306, 762, 863
HgaI GACGC 2 cut(s) 705, 906
Hin1I GRCGYC 1 cut(s) 917
Hin1II CATG 4 cut(s) 68, 118, 754, 819
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HinfI GANTC 4 cut(s) 208, 569, 798, 845
HpaII CCGG 3 cut(s) 306, 762, 863
HphI GGTGA 1 cut(s) 991
Hpy166II GTNNAC 1 cut(s) 802
Hpy188I TCNGA 4 cut(s) 314, 662, 888, 906
Hpy8I GTNNAC 1 cut(s) 802
Hpy99I CGWCG 2 cut(s) 806, 922
HpyAV CCTTC 6 cut(s) 33, 210, 223, 246, 494, 589
HpyCH4III ACNGT 2 cut(s) 228, 545
HpyCH4V TGCA 2 cut(s) 223, 361
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 358, 650, 704, 713, 839, 940, 993
HpyF3I CTNAG 5 cut(s) 15, 258, 564, 659, 834
Hsp92I GRCGYC 1 cut(s) 917
Hsp92II CATG 4 cut(s) 68, 118, 754, 819
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 267
Lsp1109I GCAGC 2 cut(s) 364, 572
LweI GCATC 2 cut(s) 376, 829
MaeI CTAG 1 cut(s) 1036
MaeIII GTNAC 2 cut(s) 398, 965
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 4 cut(s) 180, 396, 399, 1018
MfeI CAATTG 1 cut(s) 356
MluCI AATT 4 cut(s) 356, 470, 584, 1009
MlyI GAGTC 3 cut(s) 578, 807, 854
MmeI TCCRAC 2 cut(s) 760, 884
MslI CAYNNNNRTG 2 cut(s) 291, 543
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 3 cut(s) 306, 762, 863
MspR9I CCNGG 5 cut(s) 494, 725, 763, 830, 863
MunI CAATTG 1 cut(s) 356
MvaI CCWGG 3 cut(s) 494, 725, 830
MvnI CGCG 5 cut(s) 622, 648, 694, 716, 870
MwoI GCNNNNNNNGC 8 cut(s) 11, 358, 650, 704, 713, 839, 940, 993
NciI CCSGG 2 cut(s) 763, 863
NdeII GATC 1 cut(s) 103
NlaIII CATG 4 cut(s) 68, 118, 754, 819
NlaIV GGNNCC 3 cut(s) 767, 885, 944
NmeAIII GCCGAG 2 cut(s) 658, 868
NmuCI GTSAC 2 cut(s) 398, 965
NsbI TGCGCA 2 cut(s) 699, 783
NspI RCATGY 2 cut(s) 118, 819
OliI CACNNNNGTG 1 cut(s) 543
PasI CCCWGGG 1 cut(s) 493
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 5 cut(s) 354, 531, 562, 652, 715
PleI GAGTC 3 cut(s) 577, 806, 853
PpsI GAGTC 3 cut(s) 577, 806, 853
Psp6I CCWGG 3 cut(s) 492, 723, 828
PspFI CCCAGC 1 cut(s) 654
PspGI CCWGG 3 cut(s) 492, 723, 828
PspN4I GGNNCC 3 cut(s) 767, 885, 944
PspPI GGNCC 4 cut(s) 607, 670, 727, 884
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 4 cut(s) 20, 98, 854, 958
RsaNI GTAC 4 cut(s) 19, 97, 853, 957
RseI CAYNNNNRTG 2 cut(s) 291, 543
SalI GTCGAC 1 cut(s) 800
SatI GCNGC 5 cut(s) 353, 530, 561, 651, 714
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 4 cut(s) 607, 670, 727, 884
SchI GAGTC 3 cut(s) 578, 807, 854
ScrFI CCNGG 5 cut(s) 494, 725, 763, 830, 863
SfaNI GCATC 2 cut(s) 376, 829
SfcI CTRYAG 1 cut(s) 506
SinI GGWCC 3 cut(s) 607, 727, 884
SmiMI CAYNNNNRTG 2 cut(s) 291, 543
SmlI CTYRAG 1 cut(s) 1003
SmoI CTYRAG 1 cut(s) 1003
Sse9I AATT 4 cut(s) 356, 470, 584, 1009
SsiI CCGC 5 cut(s) 62, 529, 651, 714, 776
SspI AATATT 1 cut(s) 31
SspMI CTAG 1 cut(s) 1036
StyD4I CCNGG 5 cut(s) 492, 723, 761, 828, 861
StyI CCWWGG 2 cut(s) 687, 744
TaaI ACNGT 2 cut(s) 228, 545
TaqI TCGA 3 cut(s) 249, 801, 1013
TasI AATT 4 cut(s) 356, 470, 584, 1009
TatI WGTACW 1 cut(s) 956
TauI GCSGC 3 cut(s) 532, 653, 716
TfiI GAWTC 1 cut(s) 208
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 2 cut(s) 398, 965
TseI GCWGC 2 cut(s) 352, 560
Tsp45I GTSAC 2 cut(s) 398, 965
TspDTI ATGAA 1 cut(s) 63
TspRI CASTG 1 cut(s) 193
VpaK11BI GGWCC 3 cut(s) 607, 727, 884
XapI RAATTY 1 cut(s) 1009
XceI RCATGY 2 cut(s) 118, 819
XmiI GTMKAC 1 cut(s) 801
XspI CTAG 1 cut(s) 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.