FvH4_5g20850

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004388
Physical Location & Seq
Reverse (-)
12444472 .. 12446656
2185 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20850.t2

Sequence Viewer

Length: 915 bp
ATGCTGTTCAACAATGGTCTAGGAGGGAATAGTACTAATCAGCGCAAGAGAGGAAGAGAAGCTTCAGTCGCCACTGAAATCACACCTTCAATGATCCCCTTCTCTTTGCAACAGTCCCAACCTCCTCAACTCATCGACCTCTCTCAGCTCCACAATCACAATCACCCCAATGTCGTCTCCACCGGCCTCCGATTATCCTTTGGAGACCAACAACATCAACAAAATCAGCAACAATTGCAACATCACAATCAACAACAGCAACAACAACAGGATTCTCACTCTTCTTTATCTTTGTTCTCTGTTTTGACAGAGGACTTTGCCTCCCAAATCAAACAACAGAGGGACGAATTAGACCAGTTCCTTCAAGCCCAGGGGGAACAACTACAGCGCACATTAGCAGAGAAGCGGCAGAGGCACTACCGTGCGCTACTGGGCGCAGCTGAGGAGTCTGTTTCCCGTAAATTGCGAGAGAAAGAGGCAGAGTTCCAAAAGGCCACGCGCAGAAACGCCGAGTTAGAGGCACGCGCCGCCCACCTCAGCGTTGAGGCGCAGATATGGCAGGCCAAGGCGCGTGCGCAAGAGGCCACCGCCGCGTCCCTCCAGGTCCAAGTGCAACAGGCCAAGGCAATGTTGAGTGCGGGGTTCCAAGTGGCAGGTGCGCAGGATAGCAGGAGGGTCGATGATGGGCTAACATGCGCCGAAAACCAGGCGGAGGACGCTGAGTCGGGGTACATTGACCCGGAACGCGCCACCGTGTCAGGACCGAGTTGTAAAGCGTGTCGGAAGCGAGTGGCTTCGGTGGTGCTGTTGCCGTGTCGGCACCTATGCCTTTGTACAGAGTGTGACCAAACGGTGCAGTCCTGCCCATTGTGCCTCTCGATGAGAAATTCGAGTGTGGAGGTCTTTCTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

305

Amino Acids

33.89

Weight (kDa)

6.79

Isoelectric Point (pI)

62.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 226 - 262 3.9e-10 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 644
Acc16I TGCGCA 2 cut(s) 576, 660
Acc36I ACCTGC 1 cut(s) 644
AccB1I GGYRCC 1 cut(s) 819
AccII CGCG 5 cut(s) 499, 525, 571, 593, 747
AciI CCGC 6 cut(s) 406, 528, 588, 591, 638, 710
AclWI GGATC 1 cut(s) 88
AcsI RAATTY 1 cut(s) 886
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 3 cut(s) 34, 731, 835
AfiI CCNNNNNNNGG 1 cut(s) 712
AgsI TTSAA 3 cut(s) 10, 90, 365
AhdI GACNNNNNGTC 1 cut(s) 721
AjnI CCWGG 3 cut(s) 369, 600, 705
AleI CACNNNNGTG 1 cut(s) 420
AluBI AGCT 3 cut(s) 62, 148, 440
AluI AGCT 3 cut(s) 62, 148, 440
Alw26I GTCTC 2 cut(s) 181, 198
AlwI GGATC 1 cut(s) 88
AoxI GGCC 5 cut(s) 184, 492, 561, 582, 618
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 1 cut(s) 886
AspS9I GGNCC 2 cut(s) 604, 761
AsuC2I CCSGG 1 cut(s) 740
AsuHPI GGTGA 1 cut(s) 155
AvaII GGWCC 2 cut(s) 604, 761
BanI GGYRCC 1 cut(s) 819
BbvCI CCTCAGC 2 cut(s) 441, 536
BbvI GCAGC 1 cut(s) 449
BccI CCATC 1 cut(s) 677
BceAI ACGGC 1 cut(s) 796
BcgI CGANNNNNNTGC 2 cut(s) 658, 692
BciT130I CCWGG 3 cut(s) 371, 602, 707
BcnI CCSGG 1 cut(s) 740
BcoDI GTCTC 2 cut(s) 181, 198
BfaI CTAG 2 cut(s) 20, 913
BfmI CTRYAG 1 cut(s) 383
BfuAI ACCTGC 1 cut(s) 644
BglI GCCNNNNNGGC 1 cut(s) 817
BisI GCNGC 4 cut(s) 407, 438, 528, 591
BlsI GCNGC 4 cut(s) 408, 439, 529, 592
BmcAI AGTACT 1 cut(s) 34
Bme1390I CCNGG 4 cut(s) 371, 602, 707, 740
Bme18I GGWCC 2 cut(s) 604, 761
BmeRI GACNNNNNGTC 1 cut(s) 721
BmgT120I GGNCC 2 cut(s) 604, 761
BmiI GGNNCC 2 cut(s) 644, 821
BmrFI CCNGG 4 cut(s) 371, 602, 707, 740
BmrI ACTGGG 1 cut(s) 440
BmuI ACTGGG 1 cut(s) 440
BpmI CTGGAG 1 cut(s) 584
Bpu10I CCTNAGC 2 cut(s) 441, 536
BpuMI CCSGG 1 cut(s) 740
BsaI GGTCTC 1 cut(s) 198
BsaJI CCNNGG 4 cut(s) 369, 370, 564, 621
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 712
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 2 cut(s) 355, 435
Bse3DI GCAATG 1 cut(s) 633
BseBI CCWGG 3 cut(s) 371, 602, 707
BseDI CCNNGG 4 cut(s) 369, 370, 564, 621
BseLI CCNNNNNNNGG 1 cut(s) 712
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 4 cut(s) 158, 432, 550, 711
BseNI ACTGG 2 cut(s) 355, 435
BseRI GAGGAG 2 cut(s) 114, 458
BseXI GCAGC 1 cut(s) 449
BsgI GTGCAG 1 cut(s) 875
Bsh1236I CGCG 5 cut(s) 499, 525, 571, 593, 747
BshFI GGCC 5 cut(s) 186, 494, 563, 584, 620
BshNI GGYRCC 1 cut(s) 819
BsiSI CCGG 2 cut(s) 183, 740
BslFI GGGAC 3 cut(s) 100, 356, 580
BslI CCNNNNNNNGG 1 cut(s) 712
BsmAI GTCTC 2 cut(s) 181, 198
BsmBI CGTCTC 1 cut(s) 181
BsmFI GGGAC 3 cut(s) 100, 356, 580
BsnI GGCC 5 cut(s) 186, 494, 563, 584, 620
Bso31I GGTCTC 1 cut(s) 198
Bsp1407I TGTACA 1 cut(s) 833
Bsp143I GATC 1 cut(s) 93
BspACI CCGC 6 cut(s) 406, 528, 588, 591, 638, 710
BspANI GGCC 5 cut(s) 186, 494, 563, 584, 620
BspCNI CTCAG 4 cut(s) 157, 433, 549, 712
BspFNI CGCG 5 cut(s) 499, 525, 571, 593, 747
BspLI GGNNCC 2 cut(s) 644, 821
BspMI ACCTGC 1 cut(s) 644
BspPI GGATC 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 819
BspTNI GGTCTC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 633
BsrFI RCCGGY 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 833
BsrI ACTGG 2 cut(s) 355, 435
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 4 cut(s) 369, 370, 564, 621
BssMI GATC 1 cut(s) 93
BssT1I CCWWGG 2 cut(s) 564, 621
Bst2UI CCWGG 3 cut(s) 371, 602, 707
Bst4CI ACNGT 4 cut(s) 114, 422, 754, 853
Bst6I CTCTTC 2 cut(s) 49, 286
BstAPI GCANNNNNTGC 1 cut(s) 235
BstAUI TGTACA 1 cut(s) 833
BstC8I GCNNGC 3 cut(s) 523, 561, 573
BstDEI CTNAG 4 cut(s) 144, 441, 536, 720
BstFNI CGCG 5 cut(s) 499, 525, 571, 593, 747
BstKTI GATC 1 cut(s) 96
BstMAI GTCTC 2 cut(s) 181, 198
BstMBI GATC 1 cut(s) 93
BstNI CCWGG 3 cut(s) 371, 602, 707
BstNSI RCATGY 1 cut(s) 696
BstSCI CCNGG 4 cut(s) 369, 600, 705, 738
BstSFI CTRYAG 1 cut(s) 383
BstUI CGCG 5 cut(s) 499, 525, 571, 593, 747
BstV1I GCAGC 1 cut(s) 449
BsuRI GGCC 5 cut(s) 186, 494, 563, 584, 620
BtsIMutI CAGTG 1 cut(s) 72
BveI ACCTGC 1 cut(s) 644
Cac8I GCNNGC 3 cut(s) 523, 561, 573
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 2 cut(s) 604, 761
CseI GACGC 2 cut(s) 582, 725
Csp6I GTAC 3 cut(s) 33, 730, 834
CviAII CATG 1 cut(s) 693
CviQI GTAC 3 cut(s) 33, 730, 834
DdeI CTNAG 4 cut(s) 144, 441, 536, 720
DpnI GATC 1 cut(s) 95
DpnII GATC 1 cut(s) 93
DriI GACNNNNNGTC 1 cut(s) 721
Eam1104I CTCTTC 2 cut(s) 49, 286
Eam1105I GACNNNNNGTC 1 cut(s) 721
EarI CTCTTC 2 cut(s) 49, 286
EciI GGCGGA 1 cut(s) 725
Eco130I CCWWGG 2 cut(s) 564, 621
Eco31I GGTCTC 1 cut(s) 198
Eco47I GGWCC 2 cut(s) 604, 761
Eco57I CTGAAG 1 cut(s) 48
EcoRII CCWGG 3 cut(s) 369, 600, 705
EcoT14I CCWWGG 2 cut(s) 564, 621
ErhI CCWWGG 2 cut(s) 564, 621
Esp3I CGTCTC 1 cut(s) 181
FaeI CATG 1 cut(s) 696
FaiI YATR 3 cut(s) 556, 694, 826
FalI AAGNNNNNCTT 2 cut(s) 46, 78
FaqI GGGAC 3 cut(s) 100, 356, 580
FatI CATG 1 cut(s) 692
FauI CCCGC 1 cut(s) 631
Fnu4HI GCNGC 4 cut(s) 407, 438, 528, 591
Fsp4HI GCNGC 4 cut(s) 407, 438, 528, 591
FspBI CTAG 2 cut(s) 20, 913
FspI TGCGCA 2 cut(s) 576, 660
GluI GCNGC 4 cut(s) 407, 438, 528, 591
GsuI CTGGAG 1 cut(s) 584
HaeIII GGCC 5 cut(s) 186, 494, 563, 584, 620
HapII CCGG 2 cut(s) 183, 740
HgaI GACGC 2 cut(s) 582, 725
Hin1II CATG 1 cut(s) 696
HindIII AAGCTT 1 cut(s) 60
HinfI GANTC 3 cut(s) 272, 446, 722
HpaII CCGG 2 cut(s) 183, 740
HphI GGTGA 1 cut(s) 155
Hpy188I TCNGA 2 cut(s) 191, 783
Hpy188III TCNNGA 2 cut(s) 759, 877
HpyAV CCTTC 3 cut(s) 96, 109, 371
HpyCH4III ACNGT 4 cut(s) 114, 422, 754, 853
HpyCH4V TGCA 4 cut(s) 109, 238, 613, 856
HpyF3I CTNAG 4 cut(s) 144, 441, 536, 720
Hsp92II CATG 1 cut(s) 696
Kzo9I GATC 1 cut(s) 93
LmnI GCTCC 1 cut(s) 153
Lsp1109I GCAGC 1 cut(s) 449
MaeI CTAG 2 cut(s) 20, 913
MaeIII GTNAC 1 cut(s) 842
MalI GATC 1 cut(s) 95
MboI GATC 1 cut(s) 93
MboII GAAGA 2 cut(s) 66, 273
MfeI CAATTG 1 cut(s) 233
MluCI AATT 4 cut(s) 233, 347, 461, 886
MlyI GAGTC 2 cut(s) 455, 731
MmeI TCCRAC 1 cut(s) 761
MslI CAYNNNNRTG 2 cut(s) 168, 420
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 2 cut(s) 183, 740
MspR9I CCNGG 4 cut(s) 371, 602, 707, 740
MunI CAATTG 1 cut(s) 233
MvaI CCWGG 3 cut(s) 371, 602, 707
MvnI CGCG 5 cut(s) 499, 525, 571, 593, 747
NciI CCSGG 1 cut(s) 740
NdeII GATC 1 cut(s) 93
NlaIII CATG 1 cut(s) 696
NlaIV GGNNCC 2 cut(s) 644, 821
NmeAIII GCCGAG 1 cut(s) 535
NmuCI GTSAC 1 cut(s) 842
NsbI TGCGCA 2 cut(s) 576, 660
NspI RCATGY 1 cut(s) 696
OliI CACNNNNGTG 1 cut(s) 420
PaqCI CACCTGC 1 cut(s) 644
PasI CCCWGGG 1 cut(s) 370
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 4 cut(s) 408, 439, 529, 592
PleI GAGTC 2 cut(s) 454, 730
PpsI GAGTC 2 cut(s) 454, 730
Psp6I CCWGG 3 cut(s) 369, 600, 705
PspGI CCWGG 3 cut(s) 369, 600, 705
PspN4I GGNNCC 2 cut(s) 644, 821
PspPI GGNCC 2 cut(s) 604, 761
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 3 cut(s) 34, 731, 835
RsaNI GTAC 3 cut(s) 33, 730, 834
RseI CAYNNNNRTG 2 cut(s) 168, 420
SatI GCNGC 4 cut(s) 407, 438, 528, 591
Sau3AI GATC 1 cut(s) 93
Sau96I GGNCC 2 cut(s) 604, 761
ScaI AGTACT 1 cut(s) 34
SchI GAGTC 2 cut(s) 455, 731
ScrFI CCNGG 4 cut(s) 371, 602, 707, 740
SfcI CTRYAG 1 cut(s) 383
SinI GGWCC 2 cut(s) 604, 761
SmiMI CAYNNNNRTG 2 cut(s) 168, 420
Sse9I AATT 4 cut(s) 233, 347, 461, 886
SsiI CCGC 6 cut(s) 406, 528, 588, 591, 638, 710
SspMI CTAG 2 cut(s) 20, 913
StyD4I CCNGG 4 cut(s) 369, 600, 705, 738
StyI CCWWGG 2 cut(s) 564, 621
TaaI ACNGT 4 cut(s) 114, 422, 754, 853
TaqI TCGA 4 cut(s) 135, 678, 878, 890
TaqII GACCGA 1 cut(s) 778
TasI AATT 4 cut(s) 233, 347, 461, 886
TatI WGTACW 2 cut(s) 32, 833
TauI GCSGC 3 cut(s) 409, 530, 593
TfiI GAWTC 1 cut(s) 272
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 842
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 1 cut(s) 842
TspRI CASTG 1 cut(s) 79
VpaK11BI GGWCC 2 cut(s) 604, 761
XapI RAATTY 1 cut(s) 886
XceI RCATGY 1 cut(s) 696
XspI CTAG 2 cut(s) 20, 913
ZrmI AGTACT 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.