Rorug07G0099900

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004388
Physical Location & Seq
Forward (+)
7894015 .. 7897084
3070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0099900.1

Sequence Viewer

Length: 501 bp
ATGGAGCACAACCAAATACGTTTCTTTGGTTTCTCACCATCATTAGCTTCTCCTAATTTCTCATCGAGCACTGCTTCAAATATTCCTCAATTGTTTCCTCATGTTCATAGTAGCAATGCAGTTCCGATGGACAAATGCGGAACCTCCTATAGTTCAGTGAACAAATTGAAATCGATAAGGAAAGACTGTGGTGACAAGGAGACCAAGAAGCACAAAATTGCATTCCAAACAAGGAGCCAAGTTGATGTACTTGATGATGGATATCGGTGGCGAAAATATGGGCAAAAAACTGTTAAGAACAGTAACTTTCCCAGAAGTTACTACAAGTGCACACATCAAGGGTGCAATGTGAAAAAGCAAGTCCAACGCCTTTCCAAAGATGAGGAAATTGTGGTGACTACATATGAAGGAATACACATCCATCCCACTGAGAACTCTGATGAAACCTTTGAGCATATCTTAAGCCATATGAGGAAGACAGTCAAAGATGGCATAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

166

Amino Acids

19.05

Weight (kDa)

9.43

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 85 - 142 1.5e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 138
AfaI GTAC 1 cut(s) 249
AflII CTTAAG 1 cut(s) 460
AgsI TTSAA 2 cut(s) 78, 169
AluBI AGCT 1 cut(s) 47
AluI AGCT 1 cut(s) 47
Alw21I GWGCWC 3 cut(s) 9, 71, 332
Alw26I GTCTC 1 cut(s) 194
Alw44I GTGCAC 1 cut(s) 328
ApaLI GTGCAC 1 cut(s) 328
AsuHPI GGTGA 3 cut(s) 27, 203, 406
BaeGI GKGCMC 1 cut(s) 332
BbsI GAAGAC 1 cut(s) 482
Bbv12I GWGCWC 3 cut(s) 9, 71, 332
BccI CCATC 5 cut(s) 46, 121, 251, 429, 482
BcoDI GTCTC 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 148
BfrI CTTAAG 1 cut(s) 460
BmiI GGNNCC 2 cut(s) 142, 236
BpiI GAAGAC 1 cut(s) 482
Bsa29I ATCGAT 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 194
Bse3DI GCAATG 2 cut(s) 121, 352
Bse8I GATNNNNATC 1 cut(s) 261
BseCI ATCGAT 1 cut(s) 173
BseGI GGATG 2 cut(s) 417, 421
BseJI GATNNNNATC 1 cut(s) 261
BseMI GCAATG 2 cut(s) 121, 352
BseMII CTCAG 1 cut(s) 420
BseSI GKGCMC 1 cut(s) 332
BshVI ATCGAT 1 cut(s) 173
BsiHKAI GWGCWC 3 cut(s) 9, 71, 332
BsmAI GTCTC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 221
Bso31I GGTCTC 1 cut(s) 194
Bsp1286I GDGCHC 3 cut(s) 9, 71, 332
BspACI CCGC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 421
BspDI ATCGAT 1 cut(s) 173
BspLI GGNNCC 2 cut(s) 142, 236
BspTI CTTAAG 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 194
BsrDI GCAATG 2 cut(s) 121, 352
Bst4CI ACNGT 4 cut(s) 188, 292, 302, 481
BstAFI CTTAAG 1 cut(s) 460
BstDEI CTNAG 1 cut(s) 429
BstF5I GGATG 2 cut(s) 417, 421
BstMAI GTCTC 1 cut(s) 194
BstSFI CTRYAG 1 cut(s) 148
BstSLI GKGCMC 1 cut(s) 332
BstV2I GAAGAC 1 cut(s) 482
Bsu15I ATCGAT 1 cut(s) 173
BsuTUI ATCGAT 1 cut(s) 173
BtsCI GGATG 2 cut(s) 417, 421
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 3 cut(s) 69, 162, 426
ClaI ATCGAT 1 cut(s) 173
Csp6I GTAC 1 cut(s) 248
CviAII CATG 1 cut(s) 101
CviJI RGCY 3 cut(s) 47, 237, 465
CviKI_1 RGCY 3 cut(s) 47, 237, 465
CviQI GTAC 1 cut(s) 248
DdeI CTNAG 1 cut(s) 429
Eco31I GGTCTC 1 cut(s) 194
Eco32I GATATC 1 cut(s) 263
EcoRV GATATC 1 cut(s) 263
FaeI CATG 1 cut(s) 104
FatI CATG 1 cut(s) 100
FauNDI CATATG 2 cut(s) 403, 468
FokI GGATG 2 cut(s) 404, 408
Hin1II CATG 1 cut(s) 104
HphI GGTGA 3 cut(s) 27, 203, 406
Hpy166II GTNNAC 2 cut(s) 160, 330
Hpy188I TCNGA 2 cut(s) 126, 439
Hpy8I GTNNAC 2 cut(s) 160, 330
HpyAV CCTTC 1 cut(s) 401
HpyCH4III ACNGT 4 cut(s) 188, 292, 302, 481
HpyCH4IV ACGT 1 cut(s) 19
HpyCH4V TGCA 4 cut(s) 119, 221, 330, 345
HpyF3I CTNAG 1 cut(s) 429
HpySE526I ACGT 1 cut(s) 19
Hsp92II CATG 1 cut(s) 104
LmnI GCTCC 2 cut(s) 4, 234
LpnPI CCDG 1 cut(s) 325
MaeII ACGT 1 cut(s) 19
MaeIII GTNAC 4 cut(s) 191, 302, 317, 394
MboII GAAGA 1 cut(s) 487
MfeI CAATTG 1 cut(s) 89
MhlI GDGCHC 3 cut(s) 9, 71, 332
MluCI AATT 5 cut(s) 55, 89, 164, 216, 387
MmeI TCCRAC 1 cut(s) 388
MnlI CCTC 5 cut(s) 96, 108, 154, 376, 465
MseI TTAA 2 cut(s) 294, 461
MspCI CTTAAG 1 cut(s) 460
MunI CAATTG 1 cut(s) 89
Mva1269I GAATGC 1 cut(s) 221
NdeI CATATG 2 cut(s) 403, 468
NlaIII CATG 1 cut(s) 104
NlaIV GGNNCC 2 cut(s) 142, 236
NmuCI GTSAC 2 cut(s) 191, 394
PctI GAATGC 1 cut(s) 221
PspN4I GGNNCC 2 cut(s) 142, 236
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
SaqAI TTAA 2 cut(s) 294, 461
SduI GDGCHC 3 cut(s) 9, 71, 332
SetI ASST 4 cut(s) 22, 49, 146, 449
SfcI CTRYAG 1 cut(s) 148
SmlI CTYRAG 1 cut(s) 460
SmoI CTYRAG 1 cut(s) 460
Sse9I AATT 5 cut(s) 55, 89, 164, 216, 387
SsiI CCGC 1 cut(s) 138
SspI AATATT 1 cut(s) 82
TaaI ACNGT 4 cut(s) 188, 292, 302, 481
TaiI ACGT 1 cut(s) 22
TaqI TCGA 2 cut(s) 65, 173
TasI AATT 5 cut(s) 55, 89, 164, 216, 387
TatI WGTACW 1 cut(s) 247
Tru1I TTAA 2 cut(s) 294, 461
Tru9I TTAA 2 cut(s) 294, 461
TscAI CASTG 3 cut(s) 76, 162, 433
TseFI GTSAC 2 cut(s) 191, 394
Tsp45I GTSAC 2 cut(s) 191, 394
TspDTI ATGAA 3 cut(s) 95, 420, 456
TspRI CASTG 3 cut(s) 76, 162, 433
Vha464I CTTAAG 1 cut(s) 460
VneI GTGCAC 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.