RchiOBHm_Chr7g0207871

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004388
Physical Location & Seq
Reverse (-)
25178297 .. 25180748
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18602

Sequence Viewer

Length: 1035 bp
ATGGCAGTTCAAGCTCAGTACCCTTCCAATATTCTATTCCTAAACAGAAACGCACAAGAACCGCATGATTATTCATTACAAGCGCAACAGGGATTGTACCTTGATCAATCCCACATGCTATTCAACAATGGTGTAGGAGGGAATAGTACTAATCAGCGCAAGAGAGGAAGAGAAACTTCAGTTGGCACTGATATCACACCTTCAATGATTCCCTTCTCTTTGCAACCTCCTCAACTCATCGACCTCTCTCAGCTCCACAATCACAATCACAACCACAACCACCATCCCAATGTCGTCTCCACCGGCCTCCGATTATCTTTTGGAGACCAACAACAACAATTTCAACAGCATCATCACAATCAACAACAACAACAGCAGCAACAACAACAGGATTGTCACTCTTCTTCATCTCTGTTGTCTGTTTTGGCAGAGGACTTCGCCTCCCAAATCAAACAACAGAGGGACGAATTAGACCAGTTCCTTCAAGCCCAGGGGGAACAACTACAGCGCACATTAGCAGAGAAGCGGCAGACACACTACCGTGCGCTACTGGGCGCAGCTGAGGAGTCTATTTCCCGTAAATTGCGAGAGAAGGAGGCAGAGGTCCAAAAGGCCACGCGCAGGAATGCCGAGTTAGAGGCACGCGCCGCCCAGCTCAGCGTTGAGGCGCAGATATGGCAGGCCAAGGCGCGTGCGCAGGAGGCCACCGCCGCGTCCCTCCAGGTCCAAGTCCAACAGGCCAAGGCCATGATGAGCGCCGGGTTCCAAGTGGCGGGTGCGCAGGATAGCAGGAGAGCCGACGATGGGCTAACATGCGCCGAAAACCAGGCGGAGGATGCCGAGTCAGGGTACATTGACCCGGAACGCGCCACCGGGTCGGGTCCGAGTTGTAAAGCGTGTCGGAAAGGAGTGGCGTCGGTGGTGCTGTTGCCGTGTCGGCACCTATGCCTCTGTACAGAGTGTGACCAAATGGTTCAGTCCTGCCCATTGTGTCTCACCTTGAGAAATTCGAGTGTGGAAGTCTTCCTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

344

Amino Acids

38.29

Weight (kDa)

6.27

Isoelectric Point (pI)

62.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 296 - 332 2.8e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 696, 780
AccB1I GGYRCC 1 cut(s) 939
AccII CGCG 5 cut(s) 619, 645, 691, 713, 867
AciI CCGC 7 cut(s) 62, 526, 648, 708, 711, 773, 830
AcsI RAATTY 1 cut(s) 1006
AcuI CTGAAG 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 914
AfaI GTAC 5 cut(s) 20, 98, 148, 851, 955
AfiI CCNNNNNNNGG 5 cut(s) 621, 772, 804, 832, 846
AgsI TTSAA 5 cut(s) 11, 124, 204, 344, 485
AjnI CCWGG 3 cut(s) 489, 720, 825
AjuI GAANNNNNNNTTGG 4 cut(s) 20, 52, 165, 197
AleI CACNNNNGTG 1 cut(s) 540
AluBI AGCT 4 cut(s) 14, 253, 560, 655
AluI AGCT 4 cut(s) 14, 253, 560, 655
Alw26I GTCTC 3 cut(s) 301, 318, 998
AoxI GGCC 6 cut(s) 304, 612, 681, 702, 738, 744
ApeKI GCWGC 2 cut(s) 376, 557
ApoI RAATTY 1 cut(s) 1006
AspS9I GGNCC 3 cut(s) 604, 724, 881
AsuC2I CCSGG 3 cut(s) 760, 860, 874
AsuHPI GGTGA 1 cut(s) 988
AvaII GGWCC 3 cut(s) 604, 724, 881
BanI GGYRCC 1 cut(s) 939
BbsI GAAGAC 1 cut(s) 1015
BbvCI CCTCAGC 1 cut(s) 561
BbvI GCAGC 2 cut(s) 388, 569
BccI CCATC 2 cut(s) 291, 797
BceAI ACGGC 1 cut(s) 916
BciT130I CCWGG 3 cut(s) 491, 722, 827
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 3 cut(s) 760, 860, 874
BcoDI GTCTC 3 cut(s) 301, 318, 998
BfaI CTAG 1 cut(s) 1033
BfmI CTRYAG 1 cut(s) 503
BfoI RGCGCY 1 cut(s) 759
BglI GCCNNNNNGGC 1 cut(s) 937
BisI GCNGC 5 cut(s) 377, 527, 558, 648, 711
BlpI GCTNAGC 1 cut(s) 656
BlsI GCNGC 5 cut(s) 378, 528, 559, 649, 712
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 6 cut(s) 491, 722, 760, 827, 860, 874
Bme18I GGWCC 3 cut(s) 604, 724, 881
BmgT120I GGNCC 3 cut(s) 604, 724, 881
BmiI GGNNCC 3 cut(s) 764, 882, 941
BmrFI CCNGG 6 cut(s) 491, 722, 760, 827, 860, 874
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 2 cut(s) 358, 826
BmuI ACTGGG 1 cut(s) 560
BpiI GAAGAC 1 cut(s) 1015
BpmI CTGGAG 1 cut(s) 704
Bpu10I CCTNAGC 1 cut(s) 561
Bpu1102I GCTNAGC 1 cut(s) 656
BpuEI CTTGAG 1 cut(s) 1021
BpuMI CCSGG 3 cut(s) 760, 860, 874
BsaHI GRCGYC 1 cut(s) 914
BsaI GGTCTC 1 cut(s) 318
BsaJI CCNNGG 4 cut(s) 489, 490, 684, 741
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 5 cut(s) 621, 772, 804, 832, 846
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 2 cut(s) 475, 555
BseBI CCWGG 3 cut(s) 491, 722, 827
BseDI CCNNGG 4 cut(s) 489, 490, 684, 741
BseGI GGATG 2 cut(s) 283, 841
BseLI CCNNNNNNNGG 5 cut(s) 621, 772, 804, 832, 846
BseMII CTCAG 4 cut(s) 29, 263, 552, 670
BseNI ACTGG 2 cut(s) 475, 555
BseRI GAGGAG 2 cut(s) 219, 578
BseXI GCAGC 2 cut(s) 388, 569
BseYI CCCAGC 1 cut(s) 651
Bsh1236I CGCG 5 cut(s) 619, 645, 691, 713, 867
BshFI GGCC 6 cut(s) 306, 614, 683, 704, 740, 746
BshNI GGYRCC 1 cut(s) 939
BsiSI CCGG 4 cut(s) 303, 759, 860, 873
BslFI GGGAC 2 cut(s) 476, 700
BslI CCNNNNNNNGG 5 cut(s) 621, 772, 804, 832, 846
BsmAI GTCTC 3 cut(s) 301, 318, 998
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 2 cut(s) 476, 700
BsmI GAATGC 1 cut(s) 631
BsnI GGCC 6 cut(s) 306, 614, 683, 704, 740, 746
Bso31I GGTCTC 1 cut(s) 318
Bsp1407I TGTACA 1 cut(s) 953
Bsp143I GATC 1 cut(s) 103
Bsp1720I GCTNAGC 1 cut(s) 656
BspACI CCGC 7 cut(s) 62, 526, 648, 708, 711, 773, 830
BspANI GGCC 6 cut(s) 306, 614, 683, 704, 740, 746
BspCNI CTCAG 4 cut(s) 28, 262, 553, 669
BspFNI CGCG 5 cut(s) 619, 645, 691, 713, 867
BspLI GGNNCC 3 cut(s) 764, 882, 941
BspT107I GGYRCC 1 cut(s) 939
BspTNI GGTCTC 1 cut(s) 318
BsrFI RCCGGY 1 cut(s) 302
BsrGI TGTACA 1 cut(s) 953
BsrI ACTGG 2 cut(s) 475, 555
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 4 cut(s) 489, 490, 684, 741
BssMI GATC 1 cut(s) 103
BssNI GRCGYC 1 cut(s) 914
BssT1I CCWWGG 2 cut(s) 684, 741
Bst2UI CCWGG 3 cut(s) 491, 722, 827
Bst4CI ACNGT 1 cut(s) 542
Bst6I CTCTTC 2 cut(s) 163, 406
BstACI GRCGYC 1 cut(s) 914
BstAUI TGTACA 1 cut(s) 953
BstC8I GCNNGC 3 cut(s) 643, 681, 693
BstDEI CTNAG 4 cut(s) 15, 249, 561, 656
BstF5I GGATG 2 cut(s) 283, 841
BstFNI CGCG 5 cut(s) 619, 645, 691, 713, 867
BstH2I RGCGCY 1 cut(s) 759
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 3 cut(s) 301, 318, 998
BstMBI GATC 1 cut(s) 103
BstMWI GCNNNNNNNGC 7 cut(s) 11, 647, 676, 701, 710, 836, 937
BstNI CCWGG 3 cut(s) 491, 722, 827
BstNSI RCATGY 2 cut(s) 118, 816
BstSCI CCNGG 6 cut(s) 489, 720, 758, 825, 858, 872
BstSFI CTRYAG 1 cut(s) 503
BstUI CGCG 5 cut(s) 619, 645, 691, 713, 867
BstV1I GCAGC 2 cut(s) 388, 569
BstV2I GAAGAC 1 cut(s) 1015
BsuRI GGCC 6 cut(s) 306, 614, 683, 704, 740, 746
BtsCI GGATG 2 cut(s) 283, 841
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 3 cut(s) 643, 681, 693
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 3 cut(s) 604, 724, 881
CseI GACGC 2 cut(s) 702, 903
Csp6I GTAC 5 cut(s) 19, 97, 147, 850, 954
CviAII CATG 4 cut(s) 65, 115, 748, 813
CviQI GTAC 5 cut(s) 19, 97, 147, 850, 954
DdeI CTNAG 4 cut(s) 15, 249, 561, 656
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 163, 406
EarI CTCTTC 2 cut(s) 163, 406
EciI GGCGGA 1 cut(s) 845
Eco130I CCWWGG 2 cut(s) 684, 741
Eco31I GGTCTC 1 cut(s) 318
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 3 cut(s) 604, 724, 881
Eco57I CTGAAG 1 cut(s) 162
EcoRII CCWGG 3 cut(s) 489, 720, 825
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 684, 741
ErhI CCWWGG 2 cut(s) 684, 741
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 4 cut(s) 68, 118, 751, 816
FaiI YATR 6 cut(s) 66, 116, 676, 749, 814, 946
FalI AAGNNNNNCTT 3 cut(s) 160, 192, 1011
FaqI GGGAC 2 cut(s) 476, 700
FatI CATG 4 cut(s) 64, 114, 747, 812
FauI CCCGC 1 cut(s) 766
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 5 cut(s) 377, 527, 558, 648, 711
FokI GGATG 2 cut(s) 270, 848
Fsp4HI GCNGC 5 cut(s) 377, 527, 558, 648, 711
FspBI CTAG 1 cut(s) 1033
FspI TGCGCA 2 cut(s) 696, 780
GluI GCNGC 5 cut(s) 377, 527, 558, 648, 711
GsaI CCCAGC 1 cut(s) 655
GsuI CTGGAG 1 cut(s) 704
HaeII RGCGCY 1 cut(s) 759
HaeIII GGCC 6 cut(s) 306, 614, 683, 704, 740, 746
HapII CCGG 4 cut(s) 303, 759, 860, 873
HgaI GACGC 2 cut(s) 702, 903
Hin1I GRCGYC 1 cut(s) 914
Hin1II CATG 4 cut(s) 68, 118, 751, 816
HinfI GANTC 3 cut(s) 208, 566, 842
HpaII CCGG 4 cut(s) 303, 759, 860, 873
HphI GGTGA 1 cut(s) 988
Hpy188I TCNGA 3 cut(s) 311, 885, 903
Hpy99I CGWCG 2 cut(s) 803, 919
HpyAV CCTTC 5 cut(s) 33, 210, 223, 491, 586
HpyCH4III ACNGT 1 cut(s) 542
HpyCH4V TGCA 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 647, 676, 701, 710, 836, 937
HpyF3I CTNAG 4 cut(s) 15, 249, 561, 656
Hsp92I GRCGYC 1 cut(s) 914
Hsp92II CATG 4 cut(s) 68, 118, 751, 816
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 258
Lsp1109I GCAGC 2 cut(s) 388, 569
LweI GCATC 2 cut(s) 358, 826
MaeI CTAG 1 cut(s) 1033
MaeIII GTNAC 2 cut(s) 395, 962
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 4 cut(s) 180, 393, 396, 1015
MluCI AATT 4 cut(s) 338, 467, 581, 1006
MlyI GAGTC 2 cut(s) 575, 851
MmeI TCCRAC 2 cut(s) 757, 881
MslI CAYNNNNRTG 2 cut(s) 288, 540
MspA1I CMGCKG 1 cut(s) 560
MspI CCGG 4 cut(s) 303, 759, 860, 873
MspR9I CCNGG 6 cut(s) 491, 722, 760, 827, 860, 874
Mva1269I GAATGC 1 cut(s) 631
MvaI CCWGG 3 cut(s) 491, 722, 827
MvnI CGCG 5 cut(s) 619, 645, 691, 713, 867
MwoI GCNNNNNNNGC 7 cut(s) 11, 647, 676, 701, 710, 836, 937
NciI CCSGG 3 cut(s) 760, 860, 874
NdeII GATC 1 cut(s) 103
NlaIII CATG 4 cut(s) 68, 118, 751, 816
NlaIV GGNNCC 3 cut(s) 764, 882, 941
NmeAIII GCCGAG 2 cut(s) 655, 865
NmuCI GTSAC 2 cut(s) 395, 962
NsbI TGCGCA 2 cut(s) 696, 780
NspI RCATGY 2 cut(s) 118, 816
OliI CACNNNNGTG 1 cut(s) 540
PasI CCCWGGG 1 cut(s) 490
PctI GAATGC 1 cut(s) 631
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 5 cut(s) 378, 528, 559, 649, 712
PleI GAGTC 2 cut(s) 574, 850
PpsI GAGTC 2 cut(s) 574, 850
Psp6I CCWGG 3 cut(s) 489, 720, 825
PspFI CCCAGC 1 cut(s) 651
PspGI CCWGG 3 cut(s) 489, 720, 825
PspN4I GGNNCC 3 cut(s) 764, 882, 941
PspPI GGNCC 3 cut(s) 604, 724, 881
PvuII CAGCTG 1 cut(s) 560
RsaI GTAC 5 cut(s) 20, 98, 148, 851, 955
RsaNI GTAC 5 cut(s) 19, 97, 147, 850, 954
RseI CAYNNNNRTG 2 cut(s) 288, 540
SatI GCNGC 5 cut(s) 377, 527, 558, 648, 711
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 3 cut(s) 604, 724, 881
ScaI AGTACT 1 cut(s) 148
SchI GAGTC 2 cut(s) 575, 851
ScrFI CCNGG 6 cut(s) 491, 722, 760, 827, 860, 874
SfaNI GCATC 2 cut(s) 358, 826
SfcI CTRYAG 1 cut(s) 503
SinI GGWCC 3 cut(s) 604, 724, 881
SmiMI CAYNNNNRTG 2 cut(s) 288, 540
SmlI CTYRAG 1 cut(s) 1000
SmoI CTYRAG 1 cut(s) 1000
Sse9I AATT 4 cut(s) 338, 467, 581, 1006
SsiI CCGC 7 cut(s) 62, 526, 648, 708, 711, 773, 830
SspI AATATT 1 cut(s) 31
SspMI CTAG 1 cut(s) 1033
StyD4I CCNGG 6 cut(s) 489, 720, 758, 825, 858, 872
StyI CCWWGG 2 cut(s) 684, 741
TaaI ACNGT 1 cut(s) 542
TaqI TCGA 2 cut(s) 240, 1010
TasI AATT 4 cut(s) 338, 467, 581, 1006
TatI WGTACW 2 cut(s) 146, 953
TauI GCSGC 3 cut(s) 529, 650, 713
TfiI GAWTC 1 cut(s) 208
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 2 cut(s) 395, 962
TseI GCWGC 2 cut(s) 376, 557
Tsp45I GTSAC 2 cut(s) 395, 962
TspDTI ATGAA 2 cut(s) 63, 396
TspRI CASTG 1 cut(s) 193
VpaK11BI GGWCC 3 cut(s) 604, 724, 881
XapI RAATTY 1 cut(s) 1006
XceI RCATGY 2 cut(s) 118, 816
XspI CTAG 1 cut(s) 1033
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.