Prupe.8G197800_v2.0.a1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0004388
Physical Location & Seq
Forward (+)
18873262 .. 18874602
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G197800.1

Sequence Viewer

Length: 432 bp
ATGGCAGTTCAAGCTCAATACCCTTCAAATATCCTCTTTCTAAACAGAAACTCACAAGAAGGGCATGATTATTCCTTACAAGCACAGCCAGGAGGATATCTTGATCAATCCCACATGCTATTCAACAATGGAGGAGGAGCAGTCAATAATACTATCAATCAGCGCAAAAGAGGAAGAGAAACTTCAGCTGCCACTGAAATCTCACCTTCAATCCTTCCTTTCTCTCTGCAACAACAATCCCAACCTCCTCCTCCTCAGCTCATCGACCTCTCCCAACTTTACAGCAACAACCACGACCACAACAATCCCAATGTGGTCTCCACGGGCCTCCGTTTATCCTTTGGATGGTTGAGGGTAGGTACATGTACAAGAGTATGGCTGAGGGAAGGATGGGTGACGGGAGAGGGGTGGCTGAAAGGTGGGGTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

144

Amino Acids

15.86

Weight (kDa)

6.9

Isoelectric Point (pI)

65.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 2 cut(s) 361, 367
AfiI CCNNNNNNNGG 1 cut(s) 345
AflIII ACRYGT 1 cut(s) 362
AgsI TTSAA 4 cut(s) 11, 27, 124, 210
AjnI CCWGG 1 cut(s) 88
AluBI AGCT 3 cut(s) 14, 188, 259
AluI AGCT 3 cut(s) 14, 188, 259
Alw26I GTCTC 1 cut(s) 322
AoxI GGCC 1 cut(s) 325
ApeKI GCWGC 1 cut(s) 188
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 195, 406
BbvCI CCTCAGC 2 cut(s) 255, 380
BbvI GCAGC 1 cut(s) 175
BccI CCATC 2 cut(s) 339, 384
BciT130I CCWGG 1 cut(s) 90
BclI TGATCA 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 322
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 90
BmgT120I GGNCC 1 cut(s) 325
BmrFI CCNGG 1 cut(s) 90
Bpu10I CCTNAGC 2 cut(s) 255, 380
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 1 cut(s) 321
BsaXI ACNNNNNCTCC 2 cut(s) 126, 156
Bsc4I CCNNNNNNNGG 1 cut(s) 345
BseBI CCWGG 1 cut(s) 90
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 2 cut(s) 350, 395
BseLI CCNNNNNNNGG 1 cut(s) 345
BseMII CTCAG 2 cut(s) 269, 371
BseRI GAGGAG 5 cut(s) 147, 150, 237, 240, 243
BseXI GCAGC 1 cut(s) 175
BshFI GGCC 1 cut(s) 327
BslI CCNNNNNNNGG 1 cut(s) 345
BsmAI GTCTC 1 cut(s) 322
BsnI GGCC 1 cut(s) 327
Bso31I GGTCTC 1 cut(s) 322
Bsp1407I TGTACA 1 cut(s) 365
Bsp143I GATC 1 cut(s) 103
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 2 cut(s) 268, 372
BspTNI GGTCTC 1 cut(s) 322
BsrGI TGTACA 1 cut(s) 365
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 1 cut(s) 103
Bst2UI CCWGG 1 cut(s) 90
Bst6I CTCTTC 1 cut(s) 169
BstAUI TGTACA 1 cut(s) 365
BstDEI CTNAG 2 cut(s) 255, 380
BstDSI CCRYGG 1 cut(s) 321
BstF5I GGATG 2 cut(s) 350, 395
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 1 cut(s) 103
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 90
BstNSI RCATGY 2 cut(s) 118, 366
BstSCI CCNGG 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 175
BsuRI GGCC 1 cut(s) 327
BtgI CCRYGG 1 cut(s) 321
BtsCI GGATG 2 cut(s) 350, 395
BtsIMutI CAGTG 1 cut(s) 192
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 325
Csp6I GTAC 2 cut(s) 360, 366
CviAII CATG 3 cut(s) 65, 115, 363
CviJI RGCY 7 cut(s) 14, 88, 188, 259, 327, 379, 412
CviKI_1 RGCY 7 cut(s) 14, 88, 188, 259, 327, 379, 412
CviQI GTAC 2 cut(s) 360, 366
DdeI CTNAG 2 cut(s) 255, 380
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
Eam1104I CTCTTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 169
Eco31I GGTCTC 1 cut(s) 322
Eco32I GATATC 1 cut(s) 98
Eco57I CTGAAG 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 88
EcoRV GATATC 1 cut(s) 98
FaeI CATG 3 cut(s) 68, 118, 366
FaiI YATR 4 cut(s) 66, 116, 364, 376
FalI AAGNNNNNCTT 2 cut(s) 166, 198
FatI CATG 3 cut(s) 64, 114, 362
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 1 cut(s) 189
FokI GGATG 2 cut(s) 357, 402
Fsp4HI GCNGC 1 cut(s) 189
GlaI GCGC 1 cut(s) 164
GluI GCNGC 1 cut(s) 189
HaeIII GGCC 1 cut(s) 327
HhaI GCGC 1 cut(s) 165
Hin1II CATG 3 cut(s) 68, 118, 366
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HphI GGTGA 2 cut(s) 195, 406
Hpy188III TCNNGA 1 cut(s) 101
HpyAV CCTTC 5 cut(s) 33, 53, 216, 224, 380
HpyCH4V TGCA 1 cut(s) 229
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 2 cut(s) 255, 380
Hsp92II CATG 3 cut(s) 68, 118, 366
HspAI GCGC 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 137
LpnPI CCDG 2 cut(s) 75, 102
Lsp1109I GCAGC 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 394
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 188
MspR9I CCNGG 1 cut(s) 90
MvaI CCWGG 1 cut(s) 90
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 1 cut(s) 103
NlaIII CATG 3 cut(s) 68, 118, 366
NmuCI GTSAC 1 cut(s) 394
NspI RCATGY 2 cut(s) 118, 366
PciI ACATGT 1 cut(s) 362
PkrI GCNGC 1 cut(s) 190
PscI ACATGT 1 cut(s) 362
Psp6I CCWGG 1 cut(s) 88
PspGI CCWGG 1 cut(s) 88
PspPI GGNCC 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 361, 367
RsaNI GTAC 2 cut(s) 360, 366
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 1 cut(s) 325
ScrFI CCNGG 1 cut(s) 90
SetI ASST 8 cut(s) 16, 190, 208, 247, 261, 270, 361, 421
StyD4I CCNGG 1 cut(s) 88
TaqI TCGA 1 cut(s) 264
TatI WGTACW 1 cut(s) 365
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 394
TspGWI ACGGA 1 cut(s) 320
TspRI CASTG 1 cut(s) 199
XceI RCATGY 2 cut(s) 118, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.