Rh7DG239200

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0004388
Physical Location & Seq
Reverse (-)
24504723 .. 24508197
3475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG239200.1

Sequence Viewer

Length: 831 bp
ATGATTCCCTTCTCTTTGCAACCTCCTCAACTCATCGACCTCTCTCAGCTCCACAATCACAATCACAACCACAACCACCATCCCAATGTCGTCTCCACCGGCCTCCGATTATCTTTTGGAGACCAACAACAACAATTTCAACAGCATCATCACAATCAACAACAACAACAGCAGCAACAACAACAGGATTGTCACTCTTCTTCATCTCTGTTGTCTGTTTTGGCAGAGGACTTCGCCTCCCAAATCAAACAACAGAGGGACGAATTAGACCAGTTCCTTCAAGCCCAGGGGGAACAACTACAGCGCACATTAGCAGAGAAGCGGCAGACACACTACCGTGCGCTACTGGGCGCAGCTGAGGAGTCTATTTCCCGTAAATTGCGAGAGAAGGAGGCAGAGGTCCAAAAGGCCACGCGCAGGAATGCCGAGTTAGAGGCACGCGCCGCCCAGCTCAGCGTTGAGGCGCAGATATGGCAGGCCAAGGCGCGTGCGCAGGAGGCCACCGCCGCGTCCCTCCAGGTCCAAGTCCAACAGGCCAAGGCCATGATGAGCGCCGGGTTCCAAGTGGCGGGTGCGCAGGATAGCAGGAGAGCCGACGATGGGCTAACATGCGCCGAAAACCAGGCGGAGGATGCCGAGTCAGGGTACATTGACCCGGAACGCGCCACCGGGTCGGGTCCGAGTTGTAAAGCGTGTCGGAAAGGAGTGGCGTCGGTGGTGCTGTTGCCGTGTCGGCACCTATGCCTCTGTACAGAGTGTGACCAAATGGTTCAGTCCTGCCCATTGTGTCTCACCTTGAGAAATTCGAGTGTGGAAGTCTTCCTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

276

Amino Acids

30.74

Weight (kDa)

6.17

Isoelectric Point (pI)

67.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 228 - 264 2.1e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 492, 576
AccB1I GGYRCC 1 cut(s) 735
AccII CGCG 5 cut(s) 415, 441, 487, 509, 663
AciI CCGC 6 cut(s) 322, 444, 504, 507, 569, 626
AcsI RAATTY 1 cut(s) 802
AcyI GRCGYC 1 cut(s) 710
AfaI GTAC 2 cut(s) 647, 751
AfiI CCNNNNNNNGG 5 cut(s) 417, 568, 600, 628, 642
AgsI TTSAA 2 cut(s) 140, 281
AjnI CCWGG 3 cut(s) 285, 516, 621
AleI CACNNNNGTG 1 cut(s) 336
AluBI AGCT 3 cut(s) 49, 356, 451
AluI AGCT 3 cut(s) 49, 356, 451
Alw26I GTCTC 3 cut(s) 97, 114, 794
AoxI GGCC 6 cut(s) 100, 408, 477, 498, 534, 540
ApeKI GCWGC 2 cut(s) 172, 353
ApoI RAATTY 1 cut(s) 802
AspS9I GGNCC 3 cut(s) 400, 520, 677
AsuC2I CCSGG 3 cut(s) 556, 656, 670
AsuHPI GGTGA 1 cut(s) 784
AvaII GGWCC 3 cut(s) 400, 520, 677
BanI GGYRCC 1 cut(s) 735
BbsI GAAGAC 1 cut(s) 811
BbvCI CCTCAGC 1 cut(s) 357
BbvI GCAGC 2 cut(s) 184, 365
BccI CCATC 2 cut(s) 87, 593
BceAI ACGGC 1 cut(s) 712
BciT130I CCWGG 3 cut(s) 287, 518, 623
BcnI CCSGG 3 cut(s) 556, 656, 670
BcoDI GTCTC 3 cut(s) 97, 114, 794
BfaI CTAG 1 cut(s) 829
BfmI CTRYAG 1 cut(s) 299
BfoI RGCGCY 1 cut(s) 555
BglI GCCNNNNNGGC 1 cut(s) 733
BisI GCNGC 5 cut(s) 173, 323, 354, 444, 507
BlpI GCTNAGC 1 cut(s) 452
BlsI GCNGC 5 cut(s) 174, 324, 355, 445, 508
Bme1390I CCNGG 6 cut(s) 287, 518, 556, 623, 656, 670
Bme18I GGWCC 3 cut(s) 400, 520, 677
BmgT120I GGNCC 3 cut(s) 400, 520, 677
BmiI GGNNCC 3 cut(s) 560, 678, 737
BmrFI CCNGG 6 cut(s) 287, 518, 556, 623, 656, 670
BmrI ACTGGG 1 cut(s) 356
BmsI GCATC 2 cut(s) 154, 622
BmuI ACTGGG 1 cut(s) 356
BpiI GAAGAC 1 cut(s) 811
BpmI CTGGAG 1 cut(s) 500
Bpu10I CCTNAGC 1 cut(s) 357
Bpu1102I GCTNAGC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 817
BpuMI CCSGG 3 cut(s) 556, 656, 670
BsaHI GRCGYC 1 cut(s) 710
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 4 cut(s) 285, 286, 480, 537
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 5 cut(s) 417, 568, 600, 628, 642
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 2 cut(s) 271, 351
BseBI CCWGG 3 cut(s) 287, 518, 623
BseDI CCNNGG 4 cut(s) 285, 286, 480, 537
BseGI GGATG 2 cut(s) 79, 637
BseLI CCNNNNNNNGG 5 cut(s) 417, 568, 600, 628, 642
BseMII CTCAG 3 cut(s) 59, 348, 466
BseNI ACTGG 2 cut(s) 271, 351
BseRI GAGGAG 2 cut(s) 15, 374
BseXI GCAGC 2 cut(s) 184, 365
BseYI CCCAGC 1 cut(s) 447
Bsh1236I CGCG 5 cut(s) 415, 441, 487, 509, 663
BshFI GGCC 6 cut(s) 102, 410, 479, 500, 536, 542
BshNI GGYRCC 1 cut(s) 735
BsiSI CCGG 4 cut(s) 99, 555, 656, 669
BslFI GGGAC 2 cut(s) 272, 496
BslI CCNNNNNNNGG 5 cut(s) 417, 568, 600, 628, 642
BsmAI GTCTC 3 cut(s) 97, 114, 794
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 2 cut(s) 272, 496
BsmI GAATGC 1 cut(s) 427
BsnI GGCC 6 cut(s) 102, 410, 479, 500, 536, 542
Bso31I GGTCTC 1 cut(s) 114
Bsp1407I TGTACA 1 cut(s) 749
Bsp1720I GCTNAGC 1 cut(s) 452
BspACI CCGC 6 cut(s) 322, 444, 504, 507, 569, 626
BspANI GGCC 6 cut(s) 102, 410, 479, 500, 536, 542
BspCNI CTCAG 3 cut(s) 58, 349, 465
BspFNI CGCG 5 cut(s) 415, 441, 487, 509, 663
BspLI GGNNCC 3 cut(s) 560, 678, 737
BspT107I GGYRCC 1 cut(s) 735
BspTNI GGTCTC 1 cut(s) 114
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 749
BsrI ACTGG 2 cut(s) 271, 351
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 4 cut(s) 285, 286, 480, 537
BssNI GRCGYC 1 cut(s) 710
BssT1I CCWWGG 2 cut(s) 480, 537
Bst2UI CCWGG 3 cut(s) 287, 518, 623
Bst4CI ACNGT 1 cut(s) 338
Bst6I CTCTTC 1 cut(s) 202
BstACI GRCGYC 1 cut(s) 710
BstAUI TGTACA 1 cut(s) 749
BstC8I GCNNGC 3 cut(s) 439, 477, 489
BstDEI CTNAG 3 cut(s) 45, 357, 452
BstF5I GGATG 2 cut(s) 79, 637
BstFNI CGCG 5 cut(s) 415, 441, 487, 509, 663
BstH2I RGCGCY 1 cut(s) 555
BstMAI GTCTC 3 cut(s) 97, 114, 794
BstMWI GCNNNNNNNGC 6 cut(s) 443, 472, 497, 506, 632, 733
BstNI CCWGG 3 cut(s) 287, 518, 623
BstNSI RCATGY 1 cut(s) 612
BstSCI CCNGG 6 cut(s) 285, 516, 554, 621, 654, 668
BstSFI CTRYAG 1 cut(s) 299
BstUI CGCG 5 cut(s) 415, 441, 487, 509, 663
BstV1I GCAGC 2 cut(s) 184, 365
BstV2I GAAGAC 1 cut(s) 811
BsuRI GGCC 6 cut(s) 102, 410, 479, 500, 536, 542
BtsCI GGATG 2 cut(s) 79, 637
Cac8I GCNNGC 3 cut(s) 439, 477, 489
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 3 cut(s) 400, 520, 677
CseI GACGC 2 cut(s) 498, 699
Csp6I GTAC 2 cut(s) 646, 750
CviAII CATG 2 cut(s) 544, 609
CviQI GTAC 2 cut(s) 646, 750
DdeI CTNAG 3 cut(s) 45, 357, 452
Eam1104I CTCTTC 1 cut(s) 202
EarI CTCTTC 1 cut(s) 202
EciI GGCGGA 1 cut(s) 641
Eco130I CCWWGG 2 cut(s) 480, 537
Eco31I GGTCTC 1 cut(s) 114
Eco47I GGWCC 3 cut(s) 400, 520, 677
EcoRII CCWGG 3 cut(s) 285, 516, 621
EcoT14I CCWWGG 2 cut(s) 480, 537
ErhI CCWWGG 2 cut(s) 480, 537
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 2 cut(s) 547, 612
FaiI YATR 4 cut(s) 472, 545, 610, 742
FalI AAGNNNNNCTT 1 cut(s) 807
FaqI GGGAC 2 cut(s) 272, 496
FatI CATG 2 cut(s) 543, 608
FauI CCCGC 1 cut(s) 562
Fnu4HI GCNGC 5 cut(s) 173, 323, 354, 444, 507
FokI GGATG 2 cut(s) 66, 644
Fsp4HI GCNGC 5 cut(s) 173, 323, 354, 444, 507
FspBI CTAG 1 cut(s) 829
FspI TGCGCA 2 cut(s) 492, 576
GluI GCNGC 5 cut(s) 173, 323, 354, 444, 507
GsaI CCCAGC 1 cut(s) 451
GsuI CTGGAG 1 cut(s) 500
HaeII RGCGCY 1 cut(s) 555
HaeIII GGCC 6 cut(s) 102, 410, 479, 500, 536, 542
HapII CCGG 4 cut(s) 99, 555, 656, 669
HgaI GACGC 2 cut(s) 498, 699
Hin1I GRCGYC 1 cut(s) 710
Hin1II CATG 2 cut(s) 547, 612
HinfI GANTC 3 cut(s) 4, 362, 638
HpaII CCGG 4 cut(s) 99, 555, 656, 669
HphI GGTGA 1 cut(s) 784
Hpy188I TCNGA 3 cut(s) 107, 681, 699
Hpy99I CGWCG 2 cut(s) 599, 715
HpyAV CCTTC 3 cut(s) 19, 287, 382
HpyCH4III ACNGT 1 cut(s) 338
HpyCH4V TGCA 1 cut(s) 19
HpyF10VI GCNNNNNNNGC 6 cut(s) 443, 472, 497, 506, 632, 733
HpyF3I CTNAG 3 cut(s) 45, 357, 452
Hsp92I GRCGYC 1 cut(s) 710
Hsp92II CATG 2 cut(s) 547, 612
LmnI GCTCC 1 cut(s) 54
Lsp1109I GCAGC 2 cut(s) 184, 365
LweI GCATC 2 cut(s) 154, 622
MaeI CTAG 1 cut(s) 829
MaeIII GTNAC 2 cut(s) 191, 758
MboII GAAGA 3 cut(s) 189, 192, 811
MluCI AATT 4 cut(s) 134, 263, 377, 802
MlyI GAGTC 2 cut(s) 371, 647
MmeI TCCRAC 2 cut(s) 553, 677
MslI CAYNNNNRTG 2 cut(s) 84, 336
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 4 cut(s) 99, 555, 656, 669
MspR9I CCNGG 6 cut(s) 287, 518, 556, 623, 656, 670
Mva1269I GAATGC 1 cut(s) 427
MvaI CCWGG 3 cut(s) 287, 518, 623
MvnI CGCG 5 cut(s) 415, 441, 487, 509, 663
MwoI GCNNNNNNNGC 6 cut(s) 443, 472, 497, 506, 632, 733
NciI CCSGG 3 cut(s) 556, 656, 670
NlaIII CATG 2 cut(s) 547, 612
NlaIV GGNNCC 3 cut(s) 560, 678, 737
NmeAIII GCCGAG 2 cut(s) 451, 661
NmuCI GTSAC 2 cut(s) 191, 758
NsbI TGCGCA 2 cut(s) 492, 576
NspI RCATGY 1 cut(s) 612
OliI CACNNNNGTG 1 cut(s) 336
PasI CCCWGGG 1 cut(s) 286
PctI GAATGC 1 cut(s) 427
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 5 cut(s) 174, 324, 355, 445, 508
PleI GAGTC 2 cut(s) 370, 646
PpsI GAGTC 2 cut(s) 370, 646
Psp6I CCWGG 3 cut(s) 285, 516, 621
PspFI CCCAGC 1 cut(s) 447
PspGI CCWGG 3 cut(s) 285, 516, 621
PspN4I GGNNCC 3 cut(s) 560, 678, 737
PspPI GGNCC 3 cut(s) 400, 520, 677
PvuII CAGCTG 1 cut(s) 356
RsaI GTAC 2 cut(s) 647, 751
RsaNI GTAC 2 cut(s) 646, 750
RseI CAYNNNNRTG 2 cut(s) 84, 336
SatI GCNGC 5 cut(s) 173, 323, 354, 444, 507
Sau96I GGNCC 3 cut(s) 400, 520, 677
SchI GAGTC 2 cut(s) 371, 647
ScrFI CCNGG 6 cut(s) 287, 518, 556, 623, 656, 670
SetI ASST 9 cut(s) 25, 42, 51, 358, 402, 453, 522, 741, 797
SfaNI GCATC 2 cut(s) 154, 622
SfcI CTRYAG 1 cut(s) 299
SinI GGWCC 3 cut(s) 400, 520, 677
SmiMI CAYNNNNRTG 2 cut(s) 84, 336
SmlI CTYRAG 1 cut(s) 796
SmoI CTYRAG 1 cut(s) 796
Sse9I AATT 4 cut(s) 134, 263, 377, 802
SsiI CCGC 6 cut(s) 322, 444, 504, 507, 569, 626
SspMI CTAG 1 cut(s) 829
StyD4I CCNGG 6 cut(s) 285, 516, 554, 621, 654, 668
StyI CCWWGG 2 cut(s) 480, 537
TaaI ACNGT 1 cut(s) 338
TaqI TCGA 2 cut(s) 36, 806
TasI AATT 4 cut(s) 134, 263, 377, 802
TatI WGTACW 1 cut(s) 749
TauI GCSGC 3 cut(s) 325, 446, 509
TfiI GAWTC 1 cut(s) 4
TseFI GTSAC 2 cut(s) 191, 758
TseI GCWGC 2 cut(s) 172, 353
Tsp45I GTSAC 2 cut(s) 191, 758
TspDTI ATGAA 1 cut(s) 192
VpaK11BI GGWCC 3 cut(s) 400, 520, 677
XapI RAATTY 1 cut(s) 802
XceI RCATGY 1 cut(s) 612
XspI CTAG 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.