MD06G1043600.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004388
Physical Location & Seq
Forward (+)
5739327 .. 5741961
2635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1043600.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGGCAGTTCAAGCTCAATACCCTTCCAATATCCTCTTGTTTAATAGAAACTCACAAGAAGGGCATGATCATTCATTACAACCACACCCAGGAGGATATCTTGATCAATCCCACATGCTTTTCAACAATGGAGGAGGCAACACCAGTCAGCAGCGCAAAAGAGGAAGAGAAACTTCAGCAGCGACCACTGAAGATATCACTCCTCCAATCACTCCCTTCTCTTTGCAACAACAACAAGAATCCCAACCTCCTCAGCTCATCGACCTCTCTCAGCTCCACAACCACCTCCACCTACAAAATCCCAATGTCGTCTCCACCGGCCTCCGCCTATCCTTCGGAGACCAGCAACAACCACAGCAATTCCAACAGCATCACAATCAACAACACCACCACTCTTCAGCCCTCTTCTCTGCATTAACAGAGGACTTTGCCACCCAAATCAAACACCAGCGCGACGAATTAGACCAATTCCTCCAAGCCCAGGGAGAGCAGCTGCGGCGCACGTTAGCAGAGAAAAGGCAGAGGCACTACCGTGCGCTGCTAAACGCGGTGGAGGAATCAATAGCTCGCAGGCTGAGACAGAAAGAGGTAGAGGTTCAGAAGGCCACGCGCAGAAATTCCGAGTTGGAAGCAAGGGCAGCCCAACTTAGCGTTGAGGCCCAAGTTTGGCAGGCCAAGGCCCGAGCCCAACAGGCCACTGCCGCGTCTCTACAGGCCCAACTCCAGCAGGCCATGATCAGCGGAGGGTTTGCGGCCGCCCAGGATAGCAGGAGAGGGGAGGAGGGAGTAGTGTGCGCGGCGGGCGTGGAGGGCCGGGCTGAGGATGCTGAGTCGGCCTACATTGACCCGGAACGGGTGACCGTGTCGGGTCCGAGTTGTAAAGCGTGTCGGAAACGAGTTGCCTCGGTTGTTTTGTTACCTTGCCGGCATTTGAGCTTGTGTACAGAATGTGACCAAGTGATTCAGGCCTGCCCATTGTGCCTAACCTTGAGAAATTCGAGTGTGGAGGTCTATCTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

341

Amino Acids

37.9

Weight (kDa)

6.95

Isoelectric Point (pI)

68.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 292 - 328 1.4e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 453, 548, 610, 704, 797
AciI CCGC 9 cut(s) 325, 496, 548, 702, 741, 752, 756, 797, 800
AcoI YGGCCR 1 cut(s) 753
AcsI RAATTY 2 cut(s) 616, 994
AcuI CTGAAG 3 cut(s) 159, 210, 381
AfaI GTAC 1 cut(s) 943
AfiI CCNNNNNNNGG 5 cut(s) 89, 481, 666, 820, 853
AgsI TTSAA 2 cut(s) 11, 124
AjnI CCWGG 3 cut(s) 88, 480, 759
AleI CACNNNNGTG 1 cut(s) 531
AluBI AGCT 6 cut(s) 14, 256, 274, 493, 566, 936
AluI AGCT 6 cut(s) 14, 256, 274, 493, 566, 936
Alw26I GTCTC 4 cut(s) 316, 333, 571, 711
Ama87I CYCGRG 1 cut(s) 681
ApeKI GCWGC 6 cut(s) 151, 179, 490, 493, 538, 638
ApoI RAATTY 2 cut(s) 616, 994
AspLEI GCGC 6 cut(s) 156, 453, 501, 538, 612, 797
AspS9I GGNCC 5 cut(s) 658, 679, 715, 811, 869
AsuC2I CCSGG 2 cut(s) 815, 848
AsuHPI GGTGA 1 cut(s) 868
AvaI CYCGRG 1 cut(s) 681
AvaII GGWCC 1 cut(s) 869
BanII GRGCYC 1 cut(s) 688
BbvCI CCTCAGC 2 cut(s) 252, 819
BbvI GCAGC 6 cut(s) 163, 191, 480, 502, 525, 650
BciT130I CCWGG 3 cut(s) 90, 482, 761
BclI TGATCA 3 cut(s) 67, 103, 735
BcnI CCSGG 2 cut(s) 815, 848
BcoDI GTCTC 4 cut(s) 316, 333, 571, 711
BfmI CTRYAG 1 cut(s) 710
BglI GCCNNNNNGGC 1 cut(s) 692
Bme1390I CCNGG 5 cut(s) 90, 482, 761, 815, 848
Bme18I GGWCC 1 cut(s) 869
BmeT110I CYCGRG 1 cut(s) 681
BmgT120I GGNCC 5 cut(s) 658, 679, 715, 811, 869
BmiI GGNNCC 1 cut(s) 870
BmrFI CCNGG 5 cut(s) 90, 482, 761, 815, 848
BmsI GCATC 2 cut(s) 379, 814
BpmI CTGGAG 1 cut(s) 707
Bpu10I CCTNAGC 2 cut(s) 252, 819
BpuEI CTTGAG 1 cut(s) 1009
BpuMI CCSGG 2 cut(s) 815, 848
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 6 cut(s) 88, 480, 481, 675, 759, 903
Bsc4I CCNNNNNNNGG 5 cut(s) 89, 481, 666, 820, 853
Bse118I RCCGGY 2 cut(s) 317, 924
Bse1I ACTGG 1 cut(s) 144
BseBI CCWGG 3 cut(s) 90, 482, 761
BseDI CCNNGG 6 cut(s) 88, 480, 481, 675, 759, 903
BseGI GGATG 1 cut(s) 829
BseLI CCNNNNNNNGG 5 cut(s) 89, 481, 666, 820, 853
BseMII CTCAG 5 cut(s) 266, 284, 566, 810, 819
BseNI ACTGG 1 cut(s) 144
BseRI GAGGAG 4 cut(s) 147, 192, 240, 794
BseX3I CGGCCG 1 cut(s) 753
BseXI GCAGC 6 cut(s) 163, 191, 480, 502, 525, 650
Bsh1236I CGCG 5 cut(s) 453, 548, 610, 704, 797
Bsh1285I CGRYCG 1 cut(s) 756
BsiEI CGRYCG 1 cut(s) 756
BsiHKCI CYCGRG 1 cut(s) 681
BsiSI CCGG 4 cut(s) 318, 814, 848, 925
BslI CCNNNNNNNGG 5 cut(s) 89, 481, 666, 820, 853
BsmAI GTCTC 4 cut(s) 316, 333, 571, 711
BsmBI CGTCTC 2 cut(s) 316, 711
Bso31I GGTCTC 1 cut(s) 333
BsoBI CYCGRG 1 cut(s) 681
Bsp1286I GDGCHC 1 cut(s) 688
Bsp1407I TGTACA 1 cut(s) 941
Bsp143I GATC 3 cut(s) 67, 103, 735
BspACI CCGC 9 cut(s) 325, 496, 548, 702, 741, 752, 756, 797, 800
BspCNI CTCAG 5 cut(s) 265, 283, 567, 811, 820
BspFNI CGCG 5 cut(s) 453, 548, 610, 704, 797
BspLI GGNNCC 1 cut(s) 870
BspTNI GGTCTC 1 cut(s) 333
BsrFI RCCGGY 2 cut(s) 317, 924
BsrGI TGTACA 1 cut(s) 941
BsrI ACTGG 1 cut(s) 144
BssAI RCCGGY 2 cut(s) 317, 924
BssECI CCNNGG 6 cut(s) 88, 480, 481, 675, 759, 903
BssMI GATC 3 cut(s) 67, 103, 735
BssT1I CCWWGG 1 cut(s) 675
Bst2UI CCWGG 3 cut(s) 90, 482, 761
Bst4CI ACNGT 2 cut(s) 533, 862
Bst6I CTCTTC 3 cut(s) 160, 400, 410
BstAUI TGTACA 1 cut(s) 941
BstC8I GCNNGC 7 cut(s) 568, 572, 672, 729, 802, 926, 970
BstDEI CTNAG 7 cut(s) 252, 270, 575, 647, 819, 828, 1020
BstEII GGTNACC 1 cut(s) 856
BstF5I GGATG 1 cut(s) 829
BstFNI CGCG 5 cut(s) 453, 548, 610, 704, 797
BstHHI GCGC 6 cut(s) 156, 453, 501, 538, 612, 797
BstKTI GATC 3 cut(s) 70, 106, 738
BstMAI GTCTC 4 cut(s) 316, 333, 571, 711
BstMBI GATC 3 cut(s) 67, 103, 735
BstMCI CGRYCG 1 cut(s) 756
BstNI CCWGG 3 cut(s) 90, 482, 761
BstNSI RCATGY 1 cut(s) 118
BstPI GGTNACC 1 cut(s) 856
BstSCI CCNGG 5 cut(s) 88, 480, 759, 813, 846
BstSFI CTRYAG 1 cut(s) 710
BstUI CGCG 5 cut(s) 453, 548, 610, 704, 797
BstV1I GCAGC 6 cut(s) 163, 191, 480, 502, 525, 650
BstZI CGGCCG 1 cut(s) 753
BtsCI GGATG 1 cut(s) 829
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 2 cut(s) 186, 696
Cac8I GCNNGC 7 cut(s) 568, 572, 672, 729, 802, 926, 970
CciNI GCGGCCGC 1 cut(s) 753
CfoI GCGC 6 cut(s) 156, 453, 501, 538, 612, 797
Cfr10I RCCGGY 2 cut(s) 317, 924
Cfr13I GGNCC 5 cut(s) 658, 679, 715, 811, 869
CseI GACGC 1 cut(s) 693
Csp6I GTAC 1 cut(s) 942
CviAII CATG 3 cut(s) 65, 115, 733
CviQI GTAC 1 cut(s) 942
DdeI CTNAG 7 cut(s) 252, 270, 575, 647, 819, 828, 1020
DpnI GATC 3 cut(s) 69, 105, 737
DpnII GATC 3 cut(s) 67, 103, 735
EaeI YGGCCR 1 cut(s) 753
EagI CGGCCG 1 cut(s) 753
Eam1104I CTCTTC 3 cut(s) 160, 400, 410
EarI CTCTTC 3 cut(s) 160, 400, 410
EciI GGCGGA 1 cut(s) 314
EclXI CGGCCG 1 cut(s) 753
Eco130I CCWWGG 1 cut(s) 675
Eco147I AGGCCT 1 cut(s) 968
Eco24I GRGCYC 1 cut(s) 688
Eco31I GGTCTC 1 cut(s) 333
Eco32I GATATC 2 cut(s) 98, 196
Eco47I GGWCC 1 cut(s) 869
Eco52I CGGCCG 1 cut(s) 753
Eco57I CTGAAG 3 cut(s) 159, 210, 381
Eco88I CYCGRG 1 cut(s) 681
Eco91I GGTNACC 1 cut(s) 856
EcoO65I GGTNACC 1 cut(s) 856
EcoRII CCWGG 3 cut(s) 88, 480, 759
EcoRV GATATC 2 cut(s) 98, 196
EcoT14I CCWWGG 1 cut(s) 675
EcoT38I GRGCYC 1 cut(s) 688
ErhI CCWWGG 1 cut(s) 675
Esp3I CGTCTC 2 cut(s) 316, 711
FaeI CATG 3 cut(s) 68, 118, 736
FaiI YATR 3 cut(s) 66, 116, 734
FalI AAGNNNNNCTT 2 cut(s) 157, 189
FatI CATG 3 cut(s) 64, 114, 732
FauI CCCGC 1 cut(s) 793
FbaI TGATCA 3 cut(s) 67, 103, 735
FokI GGATG 1 cut(s) 836
FriOI GRGCYC 1 cut(s) 688
GlaI GCGC 6 cut(s) 155, 452, 500, 537, 611, 796
GsuI CTGGAG 1 cut(s) 707
HapII CCGG 4 cut(s) 318, 814, 848, 925
HgaI GACGC 1 cut(s) 693
HhaI GCGC 6 cut(s) 156, 453, 501, 538, 612, 797
Hin1II CATG 3 cut(s) 68, 118, 736
Hin6I GCGC 6 cut(s) 154, 451, 499, 536, 610, 795
HinP1I GCGC 6 cut(s) 154, 451, 499, 536, 610, 795
HinfI GANTC 4 cut(s) 239, 557, 830, 961
HpaII CCGG 4 cut(s) 318, 814, 848, 925
HphI GGTGA 1 cut(s) 868
Hpy166II GTNNAC 1 cut(s) 942
Hpy188I TCNGA 5 cut(s) 338, 600, 622, 873, 891
Hpy188III TCNNGA 1 cut(s) 101
Hpy8I GTNNAC 1 cut(s) 942
Hpy99I CGWCG 1 cut(s) 458
HpyAV CCTTC 5 cut(s) 33, 53, 226, 343, 595
HpyCH4III ACNGT 2 cut(s) 533, 862
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 2 cut(s) 226, 413
HpyF3I CTNAG 7 cut(s) 252, 270, 575, 647, 819, 828, 1020
HpySE526I ACGT 1 cut(s) 503
Hsp92II CATG 3 cut(s) 68, 118, 736
HspAI GCGC 6 cut(s) 154, 451, 499, 536, 610, 795
KroI GCCGGC 1 cut(s) 924
KroNI GCCGGC 1 cut(s) 926
Ksp22I TGATCA 3 cut(s) 67, 103, 735
Kzo9I GATC 3 cut(s) 67, 103, 735
LmnI GCTCC 1 cut(s) 279
Lsp1109I GCAGC 6 cut(s) 163, 191, 480, 502, 525, 650
LweI GCATC 2 cut(s) 379, 814
MaeII ACGT 1 cut(s) 503
MaeIII GTNAC 3 cut(s) 856, 915, 950
MalI GATC 3 cut(s) 69, 105, 737
MboI GATC 3 cut(s) 67, 103, 735
MboII GAAGA 4 cut(s) 177, 203, 387, 397
MhlI GDGCHC 1 cut(s) 688
MluCI AATT 5 cut(s) 359, 458, 467, 616, 994
MlyI GAGTC 1 cut(s) 839
MmeI TCCRAC 3 cut(s) 388, 606, 869
MroNI GCCGGC 1 cut(s) 924
MseI TTAA 2 cut(s) 42, 416
MslI CAYNNNNRTG 1 cut(s) 531
MspA1I CMGCKG 2 cut(s) 493, 741
MspI CCGG 4 cut(s) 318, 814, 848, 925
MspR9I CCNGG 5 cut(s) 90, 482, 761, 815, 848
MvaI CCWGG 3 cut(s) 90, 482, 761
MvnI CGCG 5 cut(s) 453, 548, 610, 704, 797
NaeI GCCGGC 1 cut(s) 926
NciI CCSGG 2 cut(s) 815, 848
NdeII GATC 3 cut(s) 67, 103, 735
NgoMIV GCCGGC 1 cut(s) 924
NlaIII CATG 3 cut(s) 68, 118, 736
NlaIV GGNNCC 1 cut(s) 870
NmuCI GTSAC 2 cut(s) 856, 950
NotI GCGGCCGC 1 cut(s) 753
NspI RCATGY 1 cut(s) 118
OliI CACNNNNGTG 1 cut(s) 531
PasI CCCWGGG 1 cut(s) 481
PceI AGGCCT 1 cut(s) 968
PdiI GCCGGC 1 cut(s) 926
PfeI GAWTC 3 cut(s) 239, 557, 961
PflFI GACNNNGTC 1 cut(s) 862
PleI GAGTC 1 cut(s) 838
PpsI GAGTC 1 cut(s) 838
Psp6I CCWGG 3 cut(s) 88, 480, 759
PspEI GGTNACC 1 cut(s) 856
PspGI CCWGG 3 cut(s) 88, 480, 759
PspN4I GGNNCC 1 cut(s) 870
PspPI GGNCC 5 cut(s) 658, 679, 715, 811, 869
PsyI GACNNNGTC 1 cut(s) 862
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 1 cut(s) 943
RsaNI GTAC 1 cut(s) 942
RseI CAYNNNNRTG 1 cut(s) 531
SaqAI TTAA 2 cut(s) 42, 416
Sau3AI GATC 3 cut(s) 67, 103, 735
Sau96I GGNCC 5 cut(s) 658, 679, 715, 811, 869
SchI GAGTC 1 cut(s) 839
ScrFI CCNGG 5 cut(s) 90, 482, 761, 815, 848
SduI GDGCHC 1 cut(s) 688
SfaNI GCATC 2 cut(s) 379, 814
SfcI CTRYAG 1 cut(s) 710
SinI GGWCC 1 cut(s) 869
SmiMI CAYNNNNRTG 1 cut(s) 531
SmlI CTYRAG 1 cut(s) 988
SmoI CTYRAG 1 cut(s) 988
Sse9I AATT 5 cut(s) 359, 458, 467, 616, 994
SseBI AGGCCT 1 cut(s) 968
SsiI CCGC 9 cut(s) 325, 496, 548, 702, 741, 752, 756, 797, 800
StuI AGGCCT 1 cut(s) 968
StyD4I CCNGG 5 cut(s) 88, 480, 759, 813, 846
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 2 cut(s) 533, 862
TaiI ACGT 1 cut(s) 506
TaqI TCGA 2 cut(s) 261, 998
TasI AATT 5 cut(s) 359, 458, 467, 616, 994
TatI WGTACW 1 cut(s) 941
TauI GCSGC 5 cut(s) 499, 704, 755, 758, 800
TfiI GAWTC 3 cut(s) 239, 557, 961
Tru1I TTAA 2 cut(s) 42, 416
Tru9I TTAA 2 cut(s) 42, 416
TscAI CASTG 2 cut(s) 193, 703
TseFI GTSAC 2 cut(s) 856, 950
TseI GCWGC 6 cut(s) 151, 179, 490, 493, 538, 638
Tsp45I GTSAC 2 cut(s) 856, 950
TspDTI ATGAA 1 cut(s) 63
TspRI CASTG 2 cut(s) 193, 703
Tth111I GACNNNGTC 1 cut(s) 862
VpaK11BI GGWCC 1 cut(s) 869
XapI RAATTY 2 cut(s) 616, 994
XceI RCATGY 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.