Rw7G020020

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004388
Physical Location & Seq
Reverse (-)
22447153 .. 22449865
2713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G020020.1

Sequence Viewer

Length: 915 bp
ATGCTATTCAACAATGGTGTAGGAGGGAATAGTACTAATCAGCGCAAGAGAGGAAGAGAAACTTCAGTTGGCACTGATATCACACCTTCAATGATTCCCTTCTCTTTGCAACAGTCCCAACCTCCTCAACTCATCGACCTCTCTCAGCTCCACAATCACAATCACAACCACAACCACCATCCCAATGTCGTCTCCACCGGCCTCCGATTATCTTTTGGAGACCAACAACAACAATTGCAACAGCATCATCACAATCAACAACAACAACAGGATTGTCACTCTTCTTCATCTCTGTTGTCTGTTTTGGCAGTGGACTTTGCCTCCCAAATCAAACAACAGAGGGACGAATTAGACCAGTTCCTTCAAGCCCAGGGGGAACAACTACAGCGCACATTAGCAGAGAAGCGGCAGACACACTACCGCGCGCTACTGGGCGCAGCTGAGGAGTCTATTTCCCGTAAATTGCGAGAGAAGGAGGCAGAGGTCCAAAAGGCCACGCGCAGAAACGCCGAGTTAGAGGCACGCGCCGCCCAGCTCAGCGTTGAGGCTCAGATATGGCAGGCCAAGGCCCGTGCGCAGGAGGCCACCGCCGCGTCCCTCCAGGTCCAAGTCCAACAGGCCAAGGCCATGATGAGCGCGGGGTTCCAAGTGGCGGGTGCGCAGGATAGCAGGAGAGCCGACGATGGGCTAACATGCGCCGAAAACCAGGCGGAGGATGCCGAGTCAGGGTACATTGACCCGGAACGCTCCACCGGGTCGGGTCCGAGTTGTAAAGCGTGTCGGAAAGGAGTGGCGTCAGTGGTGCTGTTGCCGTGTCGGCACCTATGCCTCTGCACAGAGTGTGACCAAATGGTCCAGTCCTGCCCATTGTGTCTCACCTTGAGAAATTCGAGTGTGGAAGTCTTCCTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

304

Amino Acids

33.58

Weight (kDa)

6.63

Isoelectric Point (pI)

62.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 256 - 292 2.4e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 851
Acc16I TGCGCA 2 cut(s) 576, 660
AccB1I GGYRCC 1 cut(s) 819
AccII CGCG 6 cut(s) 423, 425, 499, 525, 593, 638
AciI CCGC 8 cut(s) 406, 421, 528, 588, 591, 638, 653, 710
AcsI RAATTY 1 cut(s) 886
AcuI CTGAAG 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 794
AdeI CACNNNGTG 1 cut(s) 840
AfaI GTAC 2 cut(s) 34, 731
AfiI CCNNNNNNNGG 5 cut(s) 577, 652, 684, 712, 726
AgsI TTSAA 3 cut(s) 10, 90, 365
AjnI CCWGG 3 cut(s) 369, 600, 705
AjuI GAANNNNNNNTTGG 2 cut(s) 51, 83
AluBI AGCT 3 cut(s) 148, 440, 535
AluI AGCT 3 cut(s) 148, 440, 535
Alw26I GTCTC 3 cut(s) 196, 213, 878
AoxI GGCC 7 cut(s) 199, 492, 561, 567, 582, 618, 624
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 1 cut(s) 886
AspS9I GGNCC 5 cut(s) 484, 568, 604, 761, 853
AsuC2I CCSGG 2 cut(s) 740, 754
AsuHPI GGTGA 1 cut(s) 868
AvaII GGWCC 4 cut(s) 484, 604, 761, 853
BanI GGYRCC 1 cut(s) 819
BbsI GAAGAC 1 cut(s) 895
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 449
BccI CCATC 2 cut(s) 186, 677
BceAI ACGGC 1 cut(s) 796
BciT130I CCWGG 3 cut(s) 371, 602, 707
BcnI CCSGG 2 cut(s) 740, 754
BcoDI GTCTC 3 cut(s) 196, 213, 878
BfaI CTAG 1 cut(s) 913
BfmI CTRYAG 1 cut(s) 383
BglI GCCNNNNNGGC 1 cut(s) 817
BisI GCNGC 4 cut(s) 407, 438, 528, 591
BlpI GCTNAGC 1 cut(s) 536
BlsI GCNGC 4 cut(s) 408, 439, 529, 592
BmcAI AGTACT 1 cut(s) 34
Bme1390I CCNGG 5 cut(s) 371, 602, 707, 740, 754
Bme18I GGWCC 4 cut(s) 484, 604, 761, 853
BmgT120I GGNCC 5 cut(s) 484, 568, 604, 761, 853
BmiI GGNNCC 3 cut(s) 644, 762, 821
BmrFI CCNGG 5 cut(s) 371, 602, 707, 740, 754
BmrI ACTGGG 1 cut(s) 440
BmsI GCATC 2 cut(s) 253, 706
BmuI ACTGGG 1 cut(s) 440
BpiI GAAGAC 1 cut(s) 895
BpmI CTGGAG 1 cut(s) 584
Bpu10I CCTNAGC 1 cut(s) 441
Bpu1102I GCTNAGC 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 901
BpuMI CCSGG 2 cut(s) 740, 754
BsaHI GRCGYC 1 cut(s) 794
BsaI GGTCTC 1 cut(s) 213
BsaJI CCNNGG 4 cut(s) 369, 370, 564, 621
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 5 cut(s) 577, 652, 684, 712, 726
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 3 cut(s) 355, 435, 856
BseBI CCWGG 3 cut(s) 371, 602, 707
BseDI CCNNGG 4 cut(s) 369, 370, 564, 621
BseGI GGATG 2 cut(s) 178, 721
BseLI CCNNNNNNNGG 5 cut(s) 577, 652, 684, 712, 726
BseMII CTCAG 4 cut(s) 158, 432, 550, 563
BseNI ACTGG 3 cut(s) 355, 435, 856
BsePI GCGCGC 1 cut(s) 423
BseRI GAGGAG 2 cut(s) 114, 458
BseXI GCAGC 1 cut(s) 449
BseYI CCCAGC 1 cut(s) 531
BsgI GTGCAG 1 cut(s) 817
Bsh1236I CGCG 6 cut(s) 423, 425, 499, 525, 593, 638
BshFI GGCC 7 cut(s) 201, 494, 563, 569, 584, 620, 626
BshNI GGYRCC 1 cut(s) 819
BsiSI CCGG 3 cut(s) 198, 740, 753
BslFI GGGAC 3 cut(s) 100, 356, 580
BslI CCNNNNNNNGG 5 cut(s) 577, 652, 684, 712, 726
BsmAI GTCTC 3 cut(s) 196, 213, 878
BsmBI CGTCTC 1 cut(s) 196
BsmFI GGGAC 3 cut(s) 100, 356, 580
BsnI GGCC 7 cut(s) 201, 494, 563, 569, 584, 620, 626
Bso31I GGTCTC 1 cut(s) 213
Bsp1720I GCTNAGC 1 cut(s) 536
BspACI CCGC 8 cut(s) 406, 421, 528, 588, 591, 638, 653, 710
BspANI GGCC 7 cut(s) 201, 494, 563, 569, 584, 620, 626
BspCNI CTCAG 4 cut(s) 157, 433, 549, 562
BspFNI CGCG 6 cut(s) 423, 425, 499, 525, 593, 638
BspLI GGNNCC 3 cut(s) 644, 762, 821
BspT107I GGYRCC 1 cut(s) 819
BspTNI GGTCTC 1 cut(s) 213
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 3 cut(s) 355, 435, 856
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 4 cut(s) 369, 370, 564, 621
BssHII GCGCGC 1 cut(s) 423
BssNI GRCGYC 1 cut(s) 794
BssT1I CCWWGG 2 cut(s) 564, 621
Bst2UI CCWGG 3 cut(s) 371, 602, 707
Bst4CI ACNGT 1 cut(s) 114
Bst6I CTCTTC 2 cut(s) 49, 286
BstACI GRCGYC 1 cut(s) 794
BstC8I GCNNGC 3 cut(s) 425, 523, 561
BstDEI CTNAG 4 cut(s) 144, 441, 536, 549
BstF5I GGATG 2 cut(s) 178, 721
BstFNI CGCG 6 cut(s) 423, 425, 499, 525, 593, 638
BstMAI GTCTC 3 cut(s) 196, 213, 878
BstMWI GCNNNNNNNGC 5 cut(s) 527, 581, 590, 716, 817
BstNI CCWGG 3 cut(s) 371, 602, 707
BstNSI RCATGY 1 cut(s) 696
BstSCI CCNGG 5 cut(s) 369, 600, 705, 738, 752
BstSFI CTRYAG 1 cut(s) 383
BstUI CGCG 6 cut(s) 423, 425, 499, 525, 593, 638
BstV1I GCAGC 1 cut(s) 449
BstV2I GAAGAC 1 cut(s) 895
BsuRI GGCC 7 cut(s) 201, 494, 563, 569, 584, 620, 626
BtsCI GGATG 2 cut(s) 178, 721
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 3 cut(s) 72, 315, 804
Cac8I GCNNGC 3 cut(s) 425, 523, 561
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 5 cut(s) 484, 568, 604, 761, 853
CseI GACGC 2 cut(s) 582, 783
Csp6I GTAC 2 cut(s) 33, 730
CviAII CATG 2 cut(s) 628, 693
CviQI GTAC 2 cut(s) 33, 730
DdeI CTNAG 4 cut(s) 144, 441, 536, 549
DraIII CACNNNGTG 1 cut(s) 840
DrdI GACNNNNNNGTC 1 cut(s) 851
DseDI GACNNNNNNGTC 1 cut(s) 851
Eam1104I CTCTTC 2 cut(s) 49, 286
EarI CTCTTC 2 cut(s) 49, 286
EciI GGCGGA 1 cut(s) 725
Eco130I CCWWGG 2 cut(s) 564, 621
Eco31I GGTCTC 1 cut(s) 213
Eco32I GATATC 1 cut(s) 79
Eco47I GGWCC 4 cut(s) 484, 604, 761, 853
Eco57I CTGAAG 1 cut(s) 48
EcoRII CCWGG 3 cut(s) 369, 600, 705
EcoRV GATATC 1 cut(s) 79
EcoT14I CCWWGG 2 cut(s) 564, 621
ErhI CCWWGG 2 cut(s) 564, 621
Esp3I CGTCTC 1 cut(s) 196
FaeI CATG 2 cut(s) 631, 696
FaiI YATR 4 cut(s) 556, 629, 694, 826
FalI AAGNNNNNCTT 3 cut(s) 46, 78, 891
FaqI GGGAC 3 cut(s) 100, 356, 580
FatI CATG 2 cut(s) 627, 692
FauI CCCGC 2 cut(s) 631, 646
Fnu4HI GCNGC 4 cut(s) 407, 438, 528, 591
FokI GGATG 2 cut(s) 165, 728
Fsp4HI GCNGC 4 cut(s) 407, 438, 528, 591
FspBI CTAG 1 cut(s) 913
FspI TGCGCA 2 cut(s) 576, 660
GluI GCNGC 4 cut(s) 407, 438, 528, 591
GsaI CCCAGC 1 cut(s) 535
GsuI CTGGAG 1 cut(s) 584
HaeIII GGCC 7 cut(s) 201, 494, 563, 569, 584, 620, 626
HapII CCGG 3 cut(s) 198, 740, 753
HgaI GACGC 2 cut(s) 582, 783
Hin1I GRCGYC 1 cut(s) 794
Hin1II CATG 2 cut(s) 631, 696
HinfI GANTC 3 cut(s) 94, 446, 722
HpaII CCGG 3 cut(s) 198, 740, 753
HphI GGTGA 1 cut(s) 868
Hpy166II GTNNAC 1 cut(s) 313
Hpy188I TCNGA 4 cut(s) 206, 552, 765, 783
Hpy8I GTNNAC 1 cut(s) 313
Hpy99I CGWCG 1 cut(s) 683
HpyAV CCTTC 4 cut(s) 96, 109, 371, 466
HpyCH4III ACNGT 1 cut(s) 114
HpyCH4V TGCA 3 cut(s) 109, 238, 834
HpyF10VI GCNNNNNNNGC 5 cut(s) 527, 581, 590, 716, 817
HpyF3I CTNAG 4 cut(s) 144, 441, 536, 549
Hsp92I GRCGYC 1 cut(s) 794
Hsp92II CATG 2 cut(s) 631, 696
LmnI GCTCC 2 cut(s) 153, 752
Lsp1109I GCAGC 1 cut(s) 449
LweI GCATC 2 cut(s) 253, 706
MaeI CTAG 1 cut(s) 913
MaeIII GTNAC 2 cut(s) 275, 842
MboII GAAGA 4 cut(s) 66, 273, 276, 895
MfeI CAATTG 1 cut(s) 233
MluCI AATT 4 cut(s) 233, 347, 461, 886
MlyI GAGTC 2 cut(s) 455, 731
MmeI TCCRAC 2 cut(s) 637, 761
MslI CAYNNNNRTG 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 3 cut(s) 198, 740, 753
MspR9I CCNGG 5 cut(s) 371, 602, 707, 740, 754
MunI CAATTG 1 cut(s) 233
MvaI CCWGG 3 cut(s) 371, 602, 707
MvnI CGCG 6 cut(s) 423, 425, 499, 525, 593, 638
MwoI GCNNNNNNNGC 5 cut(s) 527, 581, 590, 716, 817
NciI CCSGG 2 cut(s) 740, 754
NlaIII CATG 2 cut(s) 631, 696
NlaIV GGNNCC 3 cut(s) 644, 762, 821
NmeAIII GCCGAG 2 cut(s) 535, 745
NmuCI GTSAC 2 cut(s) 275, 842
NsbI TGCGCA 2 cut(s) 576, 660
NspI RCATGY 1 cut(s) 696
PasI CCCWGGG 1 cut(s) 370
PauI GCGCGC 1 cut(s) 423
PfeI GAWTC 1 cut(s) 94
PkrI GCNGC 4 cut(s) 408, 439, 529, 592
PleI GAGTC 2 cut(s) 454, 730
PpsI GAGTC 2 cut(s) 454, 730
Psp6I CCWGG 3 cut(s) 369, 600, 705
PspFI CCCAGC 1 cut(s) 531
PspGI CCWGG 3 cut(s) 369, 600, 705
PspN4I GGNNCC 3 cut(s) 644, 762, 821
PspPI GGNCC 5 cut(s) 484, 568, 604, 761, 853
PteI GCGCGC 1 cut(s) 423
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 2 cut(s) 34, 731
RsaNI GTAC 2 cut(s) 33, 730
RseI CAYNNNNRTG 1 cut(s) 183
SatI GCNGC 4 cut(s) 407, 438, 528, 591
Sau96I GGNCC 5 cut(s) 484, 568, 604, 761, 853
ScaI AGTACT 1 cut(s) 34
SchI GAGTC 2 cut(s) 455, 731
ScrFI CCNGG 5 cut(s) 371, 602, 707, 740, 754
SfaNI GCATC 2 cut(s) 253, 706
SfcI CTRYAG 1 cut(s) 383
SinI GGWCC 4 cut(s) 484, 604, 761, 853
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 880
SmoI CTYRAG 1 cut(s) 880
Sse9I AATT 4 cut(s) 233, 347, 461, 886
SsiI CCGC 8 cut(s) 406, 421, 528, 588, 591, 638, 653, 710
SspMI CTAG 1 cut(s) 913
StyD4I CCNGG 5 cut(s) 369, 600, 705, 738, 752
StyI CCWWGG 2 cut(s) 564, 621
TaaI ACNGT 1 cut(s) 114
TaqI TCGA 2 cut(s) 135, 890
TasI AATT 4 cut(s) 233, 347, 461, 886
TatI WGTACW 1 cut(s) 32
TauI GCSGC 3 cut(s) 409, 530, 593
TfiI GAWTC 1 cut(s) 94
TscAI CASTG 3 cut(s) 79, 315, 804
TseFI GTSAC 2 cut(s) 275, 842
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 2 cut(s) 275, 842
TspDTI ATGAA 1 cut(s) 276
TspRI CASTG 3 cut(s) 79, 315, 804
VpaK11BI GGWCC 4 cut(s) 484, 604, 761, 853
XapI RAATTY 1 cut(s) 886
XceI RCATGY 1 cut(s) 696
XspI CTAG 1 cut(s) 913
ZrmI AGTACT 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.