Rh7BG228800

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0004388
Physical Location & Seq
Reverse (-)
19897382 .. 19900188
2807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG228800.1

Sequence Viewer

Length: 834 bp
ATGATTCCCTTCTCTTTGCAACAGTCCCAACCTCCTCAACTCATCGACCTCTCTCAGCTCCACAATCACAATCACAACCACAACCACCATCCCAATGTCGTCTCCACCGGCCTCCGATTATCTTTTGGAGACCAACAACAACATCAGCAACAATTGCAACAGCATCATCACAATCAACAACAACAACAGGATTGTCACTCTTCTTCATCTCTGTTCTCTGTTTTGGCAGAGGACTTTGCCTCCCAAATCAAACAACAGAGGGACGAATTAGACCAGTTCCTTCAAGCCCAGGGGGAACAACTACAGCGCACATTAGCAGAGAAGCGGCAGACACACTACCGTGCGCTACTGGGCGCAGCTGAGGAGTCTATTTCCCGTAAATTGCGAGAGAAGGAGGCAGAGGTCCAAAAGGCCACGCGCAGGAACGCCGAGTTAGAGGCACGCGCCGCCCAGCTCAGCGTTGAGGCGCAGATATGGCAGGCCAAGGCGCGTGCGCAGGAGGCCACAGCCGCGTCCCTCCAGGTCCAAGTCCAACAGGCCAAGGCCATGATGAGCGCCGGGTTCCAAGTGGCGGGTGCGCAGGATAGCAGGAGAGTCGACGATGGGCTAACATGCGCCGAAAACCAGGCGGAGGATGCCGAGTCAGGGTACATTGACCCGGAACGCGCCACCGGGTCGGGTCCTAGTTGTAAAGCGTGTCGGAAAGGAGTGGCCTCGGTGGTGCTGTTGCCGTGTCGGCACCTATGCCTCTGTATAGAGTGTGACCAAATGGTTCAGTCCTGCCCATTGTGCCTCACCTTGAGAAATTCGAGTGTGGAAGTCTTCCTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4_3

Protein Analysis

277

Amino Acids

30.88

Weight (kDa)

6.22

Isoelectric Point (pI)

68.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 229 - 265 5.5e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 495, 579
AccB1I GGYRCC 1 cut(s) 738
AccI GTMKAC 1 cut(s) 597
AccII CGCG 5 cut(s) 418, 444, 490, 512, 666
AciI CCGC 5 cut(s) 325, 447, 510, 572, 629
AcsI RAATTY 1 cut(s) 805
AfaI GTAC 1 cut(s) 650
AfiI CCNNNNNNNGG 4 cut(s) 420, 571, 631, 645
AgsI TTSAA 1 cut(s) 284
AjnI CCWGG 3 cut(s) 288, 519, 624
AleI CACNNNNGTG 1 cut(s) 339
AluBI AGCT 3 cut(s) 58, 359, 454
AluI AGCT 3 cut(s) 58, 359, 454
Alw26I GTCTC 2 cut(s) 106, 123
AoxI GGCC 7 cut(s) 109, 411, 480, 501, 537, 543, 711
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 1 cut(s) 805
AspS9I GGNCC 3 cut(s) 403, 523, 680
AsuC2I CCSGG 3 cut(s) 559, 659, 673
AsuHPI GGTGA 1 cut(s) 787
AvaII GGWCC 3 cut(s) 403, 523, 680
BanI GGYRCC 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 814
BbvCI CCTCAGC 1 cut(s) 360
BbvI GCAGC 1 cut(s) 368
BccI CCATC 2 cut(s) 96, 596
BceAI ACGGC 1 cut(s) 715
BcgI CGANNNNNNTGC 2 cut(s) 577, 611
BciT130I CCWGG 3 cut(s) 290, 521, 626
BcnI CCSGG 3 cut(s) 559, 659, 673
BcoDI GTCTC 2 cut(s) 106, 123
BfaI CTAG 2 cut(s) 684, 832
BfmI CTRYAG 1 cut(s) 302
BfoI RGCGCY 1 cut(s) 558
BglI GCCNNNNNGGC 1 cut(s) 736
BisI GCNGC 4 cut(s) 326, 357, 447, 510
BlpI GCTNAGC 1 cut(s) 455
BlsI GCNGC 4 cut(s) 327, 358, 448, 511
Bme1390I CCNGG 6 cut(s) 290, 521, 559, 626, 659, 673
Bme18I GGWCC 3 cut(s) 403, 523, 680
BmgT120I GGNCC 3 cut(s) 403, 523, 680
BmiI GGNNCC 3 cut(s) 563, 681, 740
BmrFI CCNGG 6 cut(s) 290, 521, 559, 626, 659, 673
BmrI ACTGGG 1 cut(s) 359
BmsI GCATC 2 cut(s) 172, 625
BmuI ACTGGG 1 cut(s) 359
BpiI GAAGAC 1 cut(s) 814
BpmI CTGGAG 1 cut(s) 503
Bpu10I CCTNAGC 1 cut(s) 360
Bpu1102I GCTNAGC 1 cut(s) 455
BpuEI CTTGAG 1 cut(s) 820
BpuMI CCSGG 3 cut(s) 559, 659, 673
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 5 cut(s) 288, 289, 483, 540, 714
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 4 cut(s) 420, 571, 631, 645
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 2 cut(s) 274, 354
BseBI CCWGG 3 cut(s) 290, 521, 626
BseDI CCNNGG 5 cut(s) 288, 289, 483, 540, 714
BseGI GGATG 2 cut(s) 88, 640
BseLI CCNNNNNNNGG 4 cut(s) 420, 571, 631, 645
BseMII CTCAG 3 cut(s) 68, 351, 469
BseNI ACTGG 2 cut(s) 274, 354
BseRI GAGGAG 2 cut(s) 24, 377
BseXI GCAGC 1 cut(s) 368
BseYI CCCAGC 1 cut(s) 450
Bsh1236I CGCG 5 cut(s) 418, 444, 490, 512, 666
BshFI GGCC 7 cut(s) 111, 413, 482, 503, 539, 545, 713
BshNI GGYRCC 1 cut(s) 738
BsiSI CCGG 4 cut(s) 108, 558, 659, 672
BslFI GGGAC 3 cut(s) 10, 275, 499
BslI CCNNNNNNNGG 4 cut(s) 420, 571, 631, 645
BsmAI GTCTC 2 cut(s) 106, 123
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 3 cut(s) 10, 275, 499
BsnI GGCC 7 cut(s) 111, 413, 482, 503, 539, 545, 713
Bso31I GGTCTC 1 cut(s) 123
Bsp1720I GCTNAGC 1 cut(s) 455
BspACI CCGC 5 cut(s) 325, 447, 510, 572, 629
BspANI GGCC 7 cut(s) 111, 413, 482, 503, 539, 545, 713
BspCNI CTCAG 3 cut(s) 67, 352, 468
BspFNI CGCG 5 cut(s) 418, 444, 490, 512, 666
BspLI GGNNCC 3 cut(s) 563, 681, 740
BspT107I GGYRCC 1 cut(s) 738
BspTNI GGTCTC 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 2 cut(s) 274, 354
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 5 cut(s) 288, 289, 483, 540, 714
BssT1I CCWWGG 2 cut(s) 483, 540
Bst2UI CCWGG 3 cut(s) 290, 521, 626
Bst4CI ACNGT 2 cut(s) 24, 341
Bst6I CTCTTC 1 cut(s) 205
BstAPI GCANNNNNTGC 1 cut(s) 154
BstC8I GCNNGC 3 cut(s) 442, 480, 492
BstDEI CTNAG 3 cut(s) 54, 360, 455
BstF5I GGATG 2 cut(s) 88, 640
BstFNI CGCG 5 cut(s) 418, 444, 490, 512, 666
BstH2I RGCGCY 1 cut(s) 558
BstMAI GTCTC 2 cut(s) 106, 123
BstMWI GCNNNNNNNGC 8 cut(s) 154, 446, 475, 500, 509, 635, 736, 789
BstNI CCWGG 3 cut(s) 290, 521, 626
BstNSI RCATGY 1 cut(s) 615
BstSCI CCNGG 6 cut(s) 288, 519, 557, 624, 657, 671
BstSFI CTRYAG 1 cut(s) 302
BstUI CGCG 5 cut(s) 418, 444, 490, 512, 666
BstV1I GCAGC 1 cut(s) 368
BstV2I GAAGAC 1 cut(s) 814
BsuRI GGCC 7 cut(s) 111, 413, 482, 503, 539, 545, 713
BtsCI GGATG 2 cut(s) 88, 640
Cac8I GCNNGC 3 cut(s) 442, 480, 492
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 3 cut(s) 403, 523, 680
CseI GACGC 1 cut(s) 501
Csp6I GTAC 1 cut(s) 649
CviAII CATG 2 cut(s) 547, 612
CviQI GTAC 1 cut(s) 649
DdeI CTNAG 3 cut(s) 54, 360, 455
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
EciI GGCGGA 1 cut(s) 644
Eco130I CCWWGG 2 cut(s) 483, 540
Eco31I GGTCTC 1 cut(s) 123
Eco47I GGWCC 3 cut(s) 403, 523, 680
EcoO109I RGGNCCY 1 cut(s) 680
EcoRII CCWGG 3 cut(s) 288, 519, 624
EcoT14I CCWWGG 2 cut(s) 483, 540
ErhI CCWWGG 2 cut(s) 483, 540
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 2 cut(s) 550, 615
FaiI YATR 5 cut(s) 475, 548, 613, 745, 755
FalI AAGNNNNNCTT 1 cut(s) 810
FaqI GGGAC 3 cut(s) 10, 275, 499
FatI CATG 2 cut(s) 546, 611
FauI CCCGC 1 cut(s) 565
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 4 cut(s) 326, 357, 447, 510
FokI GGATG 2 cut(s) 75, 647
Fsp4HI GCNGC 4 cut(s) 326, 357, 447, 510
FspBI CTAG 2 cut(s) 684, 832
FspI TGCGCA 2 cut(s) 495, 579
GluI GCNGC 4 cut(s) 326, 357, 447, 510
GsaI CCCAGC 1 cut(s) 454
GsuI CTGGAG 1 cut(s) 503
HaeII RGCGCY 1 cut(s) 558
HaeIII GGCC 7 cut(s) 111, 413, 482, 503, 539, 545, 713
HapII CCGG 4 cut(s) 108, 558, 659, 672
HgaI GACGC 1 cut(s) 501
Hin1II CATG 2 cut(s) 550, 615
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 4 cut(s) 4, 365, 594, 641
HpaII CCGG 4 cut(s) 108, 558, 659, 672
HphI GGTGA 1 cut(s) 787
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 2 cut(s) 116, 702
Hpy8I GTNNAC 1 cut(s) 598
Hpy99I CGWCG 1 cut(s) 602
HpyAV CCTTC 3 cut(s) 19, 290, 385
HpyCH4III ACNGT 2 cut(s) 24, 341
HpyCH4V TGCA 2 cut(s) 19, 157
HpyF10VI GCNNNNNNNGC 8 cut(s) 154, 446, 475, 500, 509, 635, 736, 789
HpyF3I CTNAG 3 cut(s) 54, 360, 455
Hsp92II CATG 2 cut(s) 550, 615
LmnI GCTCC 1 cut(s) 63
Lsp1109I GCAGC 1 cut(s) 368
LweI GCATC 2 cut(s) 172, 625
MaeI CTAG 2 cut(s) 684, 832
MaeIII GTNAC 2 cut(s) 194, 761
MboII GAAGA 3 cut(s) 192, 195, 814
MfeI CAATTG 1 cut(s) 152
MluCI AATT 4 cut(s) 152, 266, 380, 805
MlyI GAGTC 3 cut(s) 374, 603, 650
MmeI TCCRAC 2 cut(s) 556, 680
MslI CAYNNNNRTG 2 cut(s) 93, 339
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 4 cut(s) 108, 558, 659, 672
MspR9I CCNGG 6 cut(s) 290, 521, 559, 626, 659, 673
MunI CAATTG 1 cut(s) 152
MvaI CCWGG 3 cut(s) 290, 521, 626
MvnI CGCG 5 cut(s) 418, 444, 490, 512, 666
MwoI GCNNNNNNNGC 8 cut(s) 154, 446, 475, 500, 509, 635, 736, 789
NciI CCSGG 3 cut(s) 559, 659, 673
NlaIII CATG 2 cut(s) 550, 615
NlaIV GGNNCC 3 cut(s) 563, 681, 740
NmeAIII GCCGAG 2 cut(s) 454, 664
NmuCI GTSAC 2 cut(s) 194, 761
NsbI TGCGCA 2 cut(s) 495, 579
NspI RCATGY 1 cut(s) 615
OliI CACNNNNGTG 1 cut(s) 339
PasI CCCWGGG 1 cut(s) 289
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 4 cut(s) 327, 358, 448, 511
PleI GAGTC 3 cut(s) 373, 602, 649
PpsI GAGTC 3 cut(s) 373, 602, 649
PpuMI RGGWCCY 1 cut(s) 680
Psp5II RGGWCCY 1 cut(s) 680
Psp6I CCWGG 3 cut(s) 288, 519, 624
PspFI CCCAGC 1 cut(s) 450
PspGI CCWGG 3 cut(s) 288, 519, 624
PspN4I GGNNCC 3 cut(s) 563, 681, 740
PspPI GGNCC 3 cut(s) 403, 523, 680
PspPPI RGGWCCY 1 cut(s) 680
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 1 cut(s) 650
RsaNI GTAC 1 cut(s) 649
RseI CAYNNNNRTG 2 cut(s) 93, 339
SalI GTCGAC 1 cut(s) 596
SatI GCNGC 4 cut(s) 326, 357, 447, 510
Sau96I GGNCC 3 cut(s) 403, 523, 680
SchI GAGTC 3 cut(s) 374, 603, 650
ScrFI CCNGG 6 cut(s) 290, 521, 559, 626, 659, 673
SetI ASST 9 cut(s) 34, 51, 60, 361, 405, 456, 525, 744, 800
SfaNI GCATC 2 cut(s) 172, 625
SfcI CTRYAG 1 cut(s) 302
SinI GGWCC 3 cut(s) 403, 523, 680
SmiMI CAYNNNNRTG 2 cut(s) 93, 339
SmlI CTYRAG 1 cut(s) 799
SmoI CTYRAG 1 cut(s) 799
Sse9I AATT 4 cut(s) 152, 266, 380, 805
SsiI CCGC 5 cut(s) 325, 447, 510, 572, 629
SspMI CTAG 2 cut(s) 684, 832
StyD4I CCNGG 6 cut(s) 288, 519, 557, 624, 657, 671
StyI CCWWGG 2 cut(s) 483, 540
TaaI ACNGT 2 cut(s) 24, 341
TaqI TCGA 3 cut(s) 45, 597, 809
TasI AATT 4 cut(s) 152, 266, 380, 805
TauI GCSGC 3 cut(s) 328, 449, 512
TfiI GAWTC 1 cut(s) 4
TseFI GTSAC 2 cut(s) 194, 761
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 2 cut(s) 194, 761
TspDTI ATGAA 1 cut(s) 195
VpaK11BI GGWCC 3 cut(s) 403, 523, 680
XapI RAATTY 1 cut(s) 805
XceI RCATGY 1 cut(s) 615
XmiI GTMKAC 1 cut(s) 597
XspI CTAG 2 cut(s) 684, 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.