AT1G34220

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009241
Physical Location & Seq
Reverse (-)
12462849 .. 12466089
3241 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G34220.1

Sequence Viewer

Length: 1950 bp
ATGTCGATGCTCGATTCCTTCTTCAATAAGGGTTTCAAAGCTGCAAAATGCAAAACCTTGCTCAAGTTAACGATCCCTAGGATTAAGCTAATCAGGAATAGAAGAGAAGCACAGATTAAACAAATGCGTCGTGAAATTGCAAAGCTTCTCGAGACTGGTCAAGAAGCTACTGCTCGAATTCGGGTTGAGCATATTATAAGGGAAGAGAAGATGATGGCTGCTCAAGAGATCCTTGAGCTCTTCTGTGAACTCATTGCTGTTCGTCTTCCGATTATTGAGGCACAAAGGGAATGTCCTTTGGATCTAAAAGAAGCGATATCAAGTGTATGTTTTGCTGCGCCGAGGTGCTCTGACTTGACAGAGCTGCAGCAGGTTCAGATACTGTTTGTATCTAAGTATGGAAAGGAATTTGTTGCAGCTGCATCTGAGCTCAAGCCGGATTCTGGTGTCAATCGTAAGACTGAATCGCTGATATTCATTGCATGGTTCAGTCTAGTAGAAACAAGAGATTTGTTCATGTTTTTGTATTTTTCCAATTCAATTTTACAGTTGGTGGAGTTGCTGTCTGTACGTGCACCTTCTCCAGAAACTAAACTGAAACTTCTCAAGGAAATTGCAGAAGAACATGAACTTGATTGGGACCCTGCTTCTACAGAAACTGATCTCTTCAAATCTCATGAAGATCTCCTTGATGGACCAAAACAATTTGGTGGGGGATCTAAGCTGCCGTTACCAGAGGAACAAAATGAAAAGACAAATTTAACATCTCTTTCAGCGGCAAAGGAGAAATCTGATTCTGACTCAGAGTATGATATATTGGACTTTCCCGAGGTTCCAAATGTCTTGCTGCGACCAACTCCTGGTGCTACCTCTGTAAATGCACCGGATGCAGCGAAATCAGCCAGTTATGAACATACCTCTCACGACTTGCCCTTTGATTCAGAGAATGCAGGAGTCGAAAAAACGGCTTCCAAAAGAGATGAACATCCTGCAAAAGCAAGCAAAACTGTCGTGGAAGGCCAACAATCTTCACCCATTCTGATGGAATCTTTCGAAAAGAAGAATTACTCTCCGCCATCCATTGATGCAGTAGGGCCCATCCCTACAAAAGAGAGTGGTGCTTCCAGAGATACTCCAAGGAAAATATCCGATGGAGACTTGCAGGATGTCTTAATGGCTGCTCAAGCTGCTGCTGACTCAGCAGAACGTGCAGCATCAGCTGCCCGCTCAGCTGCAAGCCTGGCACAACTCAGAATCAACGAGCTTACCAGGAAGACTTCTGATCAGTATCCAGAAAGTCCGTCTGAGAACCCTTTCCATGCTCCCTCAATGGGAAACCTTCAATTTGATCATCAGAATTCTTCGGCCAGCAGTAGCGGAGATCTTACAGAGCTCCAGAGAGCAGAAACTTCGTCACTTTTCAACTCTGAGCAGAACAACCAACAGCCACAGACACACTCTTCAATGGAAAAACCGCAGTTTGATCGTCAGAACTCATCATTCAGTAGCTATGGCGATCTTACCCCACAGAGATTCCATTCAATGGAAAAACCACAGTTTGATCATCAGAACTCATCAGGCAGCAACTATGACGATCTTACACCACAGAGATTCTCGTCATTGGAGAAACCGCAGTTTGATCATCAGAACTCATCAGGCAGCAACTATGACGATCTTACACCACATAGATTCCCGTCAATGGAGAAACCGCAGTTTGATCATCAGAACTCATCAGTCAGCAGCTATGGCGATCTGCCAGAGCTCCAAAGGCCAGAGACTTCACCACTTGACAGACTCAGCCCTGACCAGGACCATCAGCAAATGAGACTGCCTTCAATGGAAGATGATCCCTATTACTCATACCCTAATCTCTTCACATCGCAGAAGCATGATCCCTCATCTGGCTCTCATTCGTTCTCAGACAATACAAGACCCGCCCATGATTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

649

Amino Acids

72.57

Weight (kDa)

5.12

Isoelectric Point (pI)

52.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 153 4.2e-51 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 361
AccB7I CCANNNNNTGG 2 cut(s) 860, 1543
AccBSI CCGCTC 1 cut(s) 1227
AciI CCGC 8 cut(s) 776, 1073, 1225, 1377, 1475, 1631, 1709, 1935
AclWI GGATC 6 cut(s) 67, 223, 309, 724, 1841, 1886
AcoI YGGCCR 1 cut(s) 1365
AcsI RAATTY 4 cut(s) 177, 407, 757, 1357
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 7 cut(s) 443, 860, 1331, 1543, 1699, 1807, 1901
AgsI TTSAA 9 cut(s) 25, 37, 540, 670, 1343, 1423, 1464, 1542, 1836
AjnI CCWGG 4 cut(s) 859, 1239, 1268, 1806
Alw21I GWGCWC 6 cut(s) 240, 350, 432, 577, 1395, 1764
Alw26I GTCTC 4 cut(s) 146, 1149, 1769, 1819
Alw44I GTGCAC 1 cut(s) 573
AlwI GGATC 6 cut(s) 67, 223, 309, 724, 1841, 1886
AlwNI CAGNNNCTG 2 cut(s) 382, 659
Ama87I CYCGRG 2 cut(s) 149, 827
AoxI GGCC 4 cut(s) 1018, 1094, 1365, 1769
ApaI GGGCCC 1 cut(s) 1098
ApaLI GTGCAC 1 cut(s) 573
ApoI RAATTY 4 cut(s) 177, 407, 757, 1357
ArsI GACNNNNNNTTYG 4 cut(s) 277, 309, 917, 949
Asp700I GAANNNNTTC 1 cut(s) 1313
AspA2I CCTAGG 1 cut(s) 77
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 5 cut(s) 640, 695, 1094, 1095, 1810
AsuHPI GGTGA 2 cut(s) 1023, 1773
AsuII TTCGAA 1 cut(s) 1053
AvaI CYCGRG 2 cut(s) 149, 827
AvaII GGWCC 3 cut(s) 640, 695, 1810
AvrII CCTAGG 1 cut(s) 77
BaeGI GKGCMC 2 cut(s) 577, 1098
BanII GRGCYC 5 cut(s) 240, 432, 1098, 1395, 1764
BbsI GAAGAC 2 cut(s) 257, 1280
Bbv12I GWGCWC 6 cut(s) 240, 350, 432, 577, 1395, 1764
BccI CCATC 7 cut(s) 208, 686, 1036, 1084, 1106, 1145, 1821
BceAI ACGGC 2 cut(s) 712, 981
BcgI CGANNNNNNTGC 6 cut(s) 991, 1025, 1392, 1426, 1466, 1500
BciT130I CCWGG 4 cut(s) 861, 1241, 1270, 1808
BciVI GTATCC 1 cut(s) 1299
BclI TGATCA 5 cut(s) 1282, 1348, 1561, 1639, 1717
BcoDI GTCTC 4 cut(s) 146, 1149, 1769, 1819
BfaI CTAG 2 cut(s) 78, 494
BfmI CTRYAG 2 cut(s) 365, 651
BfuAI ACCTGC 1 cut(s) 361
BfuI GTATCC 1 cut(s) 1299
BglII AGATCT 2 cut(s) 682, 1381
BlnI CCTAGG 1 cut(s) 77
BlpI GCTNAGC 1 cut(s) 1228
Bme1390I CCNGG 4 cut(s) 861, 1241, 1270, 1808
Bme18I GGWCC 3 cut(s) 640, 695, 1810
BmeT110I CYCGRG 2 cut(s) 149, 827
BmgT120I GGNCC 5 cut(s) 640, 695, 1094, 1095, 1810
BmiI GGNNCC 4 cut(s) 641, 642, 834, 1096
BmrFI CCNGG 4 cut(s) 861, 1241, 1270, 1808
BmsI GCATC 4 cut(s) 431, 877, 1075, 1223
BpiI GAAGAC 2 cut(s) 257, 1280
BpmI CTGGAG 2 cut(s) 567, 1379
Bpu1102I GCTNAGC 1 cut(s) 1228
Bpu14I TTCGAA 1 cut(s) 1053
BpuEI CTTGAG 6 cut(s) 47, 207, 254, 416, 590, 1167
BsaAI YACGTR 1 cut(s) 572
BsaBI GATNNNNATC 2 cut(s) 984, 1287
BsaJI CCNNGG 4 cut(s) 77, 341, 828, 1136
BsaWI WCCGGW 1 cut(s) 883
BsaXI ACNNNNNCTCC 2 cut(s) 548, 578
Bsc4I CCNNNNNNNGG 7 cut(s) 443, 860, 1331, 1543, 1699, 1807, 1901
Bse1I ACTGG 2 cut(s) 160, 903
Bse3DI GCAATG 2 cut(s) 252, 477
Bse8I GATNNNNATC 2 cut(s) 984, 1287
BseBI CCWGG 4 cut(s) 861, 1241, 1270, 1808
BseDI CCNNGG 4 cut(s) 77, 341, 828, 1136
BseGI GGATG 5 cut(s) 892, 985, 1076, 1098, 1171
BseJI GATNNNNATC 2 cut(s) 984, 1287
BseLI CCNNNNNNNGG 7 cut(s) 443, 860, 1331, 1543, 1699, 1807, 1901
BseMI GCAATG 2 cut(s) 252, 477
BseMII CTCAG 9 cut(s) 417, 816, 1212, 1242, 1264, 1296, 1419, 1810, 1932
BseNI ACTGG 2 cut(s) 160, 903
BseSI GKGCMC 2 cut(s) 577, 1098
BsgI GTGCAG 1 cut(s) 1230
BshFI GGCC 4 cut(s) 1020, 1096, 1367, 1771
BsiHKAI GWGCWC 6 cut(s) 240, 350, 432, 577, 1395, 1764
BsiHKCI CYCGRG 2 cut(s) 149, 827
BsiSI CCGG 2 cut(s) 437, 884
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 7 cut(s) 443, 860, 1331, 1543, 1699, 1807, 1901
BsmAI GTCTC 4 cut(s) 146, 1149, 1769, 1819
BsmFI GGGAC 1 cut(s) 653
BsmI GAATGC 1 cut(s) 952
BsnI GGCC 4 cut(s) 1020, 1096, 1367, 1771
BsoBI CYCGRG 2 cut(s) 149, 827
Bsp119I TTCGAA 1 cut(s) 1053
Bsp120I GGGCCC 1 cut(s) 1094
Bsp1286I GDGCHC 7 cut(s) 240, 350, 432, 577, 1098, 1395, 1764
Bsp1720I GCTNAGC 1 cut(s) 1228
BspACI CCGC 8 cut(s) 776, 1073, 1225, 1377, 1475, 1631, 1709, 1935
BspANI GGCC 4 cut(s) 1020, 1096, 1367, 1771
BspCNI CTCAG 9 cut(s) 418, 815, 1211, 1241, 1263, 1297, 1420, 1809, 1931
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 4 cut(s) 641, 642, 834, 1096
BspMAI CTGCAG 1 cut(s) 369
BspMI ACCTGC 1 cut(s) 361
BspPI GGATC 6 cut(s) 67, 223, 309, 724, 1841, 1886
BspQI GCTCTTC 1 cut(s) 245
BspT104I TTCGAA 1 cut(s) 1053
BsrBI CCGCTC 1 cut(s) 1227
BsrDI GCAATG 2 cut(s) 252, 477
BsrI ACTGG 2 cut(s) 160, 903
BssECI CCNNGG 4 cut(s) 77, 341, 828, 1136
BssT1I CCWWGG 2 cut(s) 77, 1136
Bst2UI CCWGG 4 cut(s) 861, 1241, 1270, 1808
Bst4CI ACNGT 4 cut(s) 384, 549, 1009, 1557
Bst6I CTCTTC 6 cut(s) 97, 198, 245, 671, 1465, 1877
BstAPI GCANNNNNTGC 3 cut(s) 887, 1208, 1220
BstBAI YACGTR 1 cut(s) 572
BstBI TTCGAA 1 cut(s) 1053
BstC8I GCNNGC 4 cut(s) 1000, 1225, 1237, 1369
BstF5I GGATG 5 cut(s) 892, 985, 1076, 1098, 1171
BstHHI GCGC 1 cut(s) 340
BstMAI GTCTC 4 cut(s) 146, 1149, 1769, 1819
BstNI CCWGG 4 cut(s) 861, 1241, 1270, 1808
BstSCI CCNGG 4 cut(s) 859, 1239, 1268, 1806
BstSFI CTRYAG 2 cut(s) 365, 651
BstSLI GKGCMC 2 cut(s) 577, 1098
BstV2I GAAGAC 2 cut(s) 257, 1280
BstX2I RGATCY 5 cut(s) 228, 301, 682, 716, 1381
BstXI CCANNNNNNTGG 1 cut(s) 1042
BstYI RGATCY 5 cut(s) 228, 301, 682, 716, 1381
BsuI GTATCC 1 cut(s) 1299
BsuRI GGCC 4 cut(s) 1020, 1096, 1367, 1771
BtgZI GCGATG 1 cut(s) 1863
BtsCI GGATG 5 cut(s) 892, 985, 1076, 1098, 1171
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 4 cut(s) 1000, 1225, 1237, 1369
CaiI CAGNNNCTG 2 cut(s) 382, 659
CciI TCATGA 1 cut(s) 676
CfoI GCGC 1 cut(s) 340
Cfr13I GGNCC 5 cut(s) 640, 695, 1094, 1095, 1810
CseI GACGC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 569
CviAII CATG 7 cut(s) 483, 517, 626, 677, 1319, 1889, 1940
CviQI GTAC 1 cut(s) 569
EaeI YGGCCR 1 cut(s) 1365
Eam1104I CTCTTC 6 cut(s) 97, 198, 245, 671, 1465, 1877
EarI CTCTTC 6 cut(s) 97, 198, 245, 671, 1465, 1877
EciI GGCGGA 1 cut(s) 1062
Ecl136II GAGCTC 4 cut(s) 238, 430, 1393, 1762
Eco130I CCWWGG 2 cut(s) 77, 1136
Eco24I GRGCYC 5 cut(s) 240, 432, 1098, 1395, 1764
Eco32I GATATC 1 cut(s) 318
Eco47I GGWCC 3 cut(s) 640, 695, 1810
Eco53kI GAGCTC 4 cut(s) 238, 430, 1393, 1762
Eco88I CYCGRG 2 cut(s) 149, 827
EcoICRI GAGCTC 4 cut(s) 238, 430, 1393, 1762
EcoO109I RGGNCCY 2 cut(s) 640, 1094
EcoRI GAATTC 2 cut(s) 177, 1357
EcoRII CCWGG 4 cut(s) 859, 1239, 1268, 1806
EcoRV GATATC 1 cut(s) 318
EcoT14I CCWWGG 2 cut(s) 77, 1136
EcoT38I GRGCYC 5 cut(s) 240, 432, 1098, 1395, 1764
ErhI CCWWGG 2 cut(s) 77, 1136
FaeI CATG 7 cut(s) 486, 520, 629, 680, 1322, 1892, 1943
FalI AAGNNNNNCTT 4 cut(s) 216, 248, 672, 704
FaqI GGGAC 1 cut(s) 653
FatI CATG 7 cut(s) 482, 516, 625, 676, 1318, 1888, 1939
FauI CCCGC 2 cut(s) 1232, 1942
FbaI TGATCA 5 cut(s) 1282, 1348, 1561, 1639, 1717
FokI GGATG 5 cut(s) 899, 972, 1063, 1085, 1178
FriOI GRGCYC 5 cut(s) 240, 432, 1098, 1395, 1764
FspBI CTAG 2 cut(s) 78, 494
GlaI GCGC 1 cut(s) 339
GsuI CTGGAG 2 cut(s) 567, 1379
HaeIII GGCC 4 cut(s) 1020, 1096, 1367, 1771
HapII CCGG 2 cut(s) 437, 884
HgaI GACGC 1 cut(s) 116
HhaI GCGC 1 cut(s) 340
Hin1II CATG 7 cut(s) 486, 520, 629, 680, 1322, 1892, 1943
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HindIII AAGCTT 1 cut(s) 143
HpaI GTTAAC 1 cut(s) 69
HpaII CCGG 2 cut(s) 437, 884
HphI GGTGA 2 cut(s) 1023, 1773
Hpy166II GTNNAC 3 cut(s) 69, 248, 575
Hpy8I GTNNAC 3 cut(s) 69, 248, 575
Hpy99I CGWCG 1 cut(s) 132
HpyAV CCTTC 5 cut(s) 28, 588, 1010, 1349, 1842
HpyCH4III ACNGT 4 cut(s) 384, 549, 1009, 1557
HpyCH4IV ACGT 2 cut(s) 571, 1207
HpySE526I ACGT 2 cut(s) 571, 1207
Hsp92II CATG 7 cut(s) 486, 520, 629, 680, 1322, 1892, 1943
HspAI GCGC 1 cut(s) 338
KflI GGGWCCC 1 cut(s) 640
Ksp22I TGATCA 5 cut(s) 1282, 1348, 1561, 1639, 1717
KspAI GTTAAC 1 cut(s) 69
LguI GCTCTTC 1 cut(s) 245
LmnI GCTCC 3 cut(s) 1327, 1398, 1767
LweI GCATC 4 cut(s) 431, 877, 1075, 1223
MaeI CTAG 2 cut(s) 78, 494
MaeII ACGT 2 cut(s) 571, 1207
MaeIII GTNAC 2 cut(s) 729, 1413
MbiI CCGCTC 1 cut(s) 1227
MflI RGATCY 5 cut(s) 228, 301, 682, 716, 1381
MhlI GDGCHC 7 cut(s) 240, 350, 432, 577, 1098, 1395, 1764
MlyI GAGTC 4 cut(s) 794, 963, 1190, 1788
MnlI CCTC 8 cut(s) 271, 336, 730, 823, 880, 928, 1336, 1906
MroXI GAANNNNTTC 1 cut(s) 1313
MseI TTAA 5 cut(s) 68, 84, 117, 761, 1172
MslI CAYNNNNRTG 1 cut(s) 1040
MspA1I CMGCKG 4 cut(s) 419, 776, 1220, 1232
MspI CCGG 2 cut(s) 437, 884
MspR9I CCNGG 4 cut(s) 861, 1241, 1270, 1808
Mva1269I GAATGC 1 cut(s) 952
MvaI CCWGG 4 cut(s) 861, 1241, 1270, 1808
NlaIII CATG 7 cut(s) 486, 520, 629, 680, 1322, 1892, 1943
NlaIV GGNNCC 4 cut(s) 641, 642, 834, 1096
NmeAIII GCCGAG 1 cut(s) 366
NmuCI GTSAC 1 cut(s) 1413
NspV TTCGAA 1 cut(s) 1053
PaeR7I CTCGAG 1 cut(s) 149
PagI TCATGA 1 cut(s) 676
PciSI GCTCTTC 1 cut(s) 245
PctI GAATGC 1 cut(s) 952
PdmI GAANNNNTTC 1 cut(s) 1313
PflMI CCANNNNNTGG 2 cut(s) 860, 1543
PleI GAGTC 4 cut(s) 794, 962, 1190, 1788
PpsI GAGTC 4 cut(s) 794, 962, 1190, 1788
Ppu21I YACGTR 1 cut(s) 572
PpuMI RGGWCCY 1 cut(s) 640
PsiI TTATAA 1 cut(s) 197
Psp124BI GAGCTC 4 cut(s) 240, 432, 1395, 1764
Psp5II RGGWCCY 1 cut(s) 640
Psp6I CCWGG 4 cut(s) 859, 1239, 1268, 1806
PspGI CCWGG 4 cut(s) 859, 1239, 1268, 1806
PspN4I GGNNCC 4 cut(s) 641, 642, 834, 1096
PspOMI GGGCCC 1 cut(s) 1094
PspPI GGNCC 5 cut(s) 640, 695, 1094, 1095, 1810
PspPPI RGGWCCY 1 cut(s) 640
PstI CTGCAG 1 cut(s) 369
PstNI CAGNNNCTG 2 cut(s) 382, 659
PsuI RGATCY 5 cut(s) 228, 301, 682, 716, 1381
PvuII CAGCTG 3 cut(s) 419, 1220, 1232
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
RseI CAYNNNNRTG 1 cut(s) 1040
SacI GAGCTC 4 cut(s) 240, 432, 1395, 1764
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 5 cut(s) 68, 84, 117, 761, 1172
Sau96I GGNCC 5 cut(s) 640, 695, 1094, 1095, 1810
SchI GAGTC 4 cut(s) 794, 963, 1190, 1788
ScrFI CCNGG 4 cut(s) 861, 1241, 1270, 1808
SduI GDGCHC 7 cut(s) 240, 350, 432, 577, 1098, 1395, 1764
SfaNI GCATC 4 cut(s) 431, 877, 1075, 1223
SfcI CTRYAG 2 cut(s) 365, 651
Sfr274I CTCGAG 1 cut(s) 149
SfuI TTCGAA 1 cut(s) 1053
SinI GGWCC 3 cut(s) 640, 695, 1810
SlaI CTCGAG 1 cut(s) 149
SmiMI CAYNNNNRTG 1 cut(s) 1040
SmlI CTYRAG 7 cut(s) 62, 149, 222, 233, 431, 605, 1182
SmoI CTYRAG 7 cut(s) 62, 149, 222, 233, 431, 605, 1182
SsiI CCGC 8 cut(s) 776, 1073, 1225, 1377, 1475, 1631, 1709, 1935
SspMI CTAG 2 cut(s) 78, 494
SstI GAGCTC 4 cut(s) 240, 432, 1395, 1764
StyD4I CCNGG 4 cut(s) 859, 1239, 1268, 1806
StyI CCWWGG 2 cut(s) 77, 1136
TaaI ACNGT 4 cut(s) 384, 549, 1009, 1557
TaiI ACGT 2 cut(s) 574, 1210
TaqI TCGA 6 cut(s) 5, 12, 150, 175, 957, 1053
TauI GCSGC 1 cut(s) 779
Tru1I TTAA 5 cut(s) 68, 84, 117, 761, 1172
Tru9I TTAA 5 cut(s) 68, 84, 117, 761, 1172
TseFI GTSAC 1 cut(s) 1413
Tsp45I GTSAC 1 cut(s) 1413
TspDTI ATGAA 7 cut(s) 466, 505, 642, 693, 762, 924, 996
TspGWI ACGGA 1 cut(s) 1290
Van91I CCANNNNNTGG 2 cut(s) 860, 1543
VneI GTGCAC 1 cut(s) 573
VpaK11BI GGWCC 3 cut(s) 640, 695, 1810
XapI RAATTY 4 cut(s) 177, 407, 757, 1357
XhoI CTCGAG 1 cut(s) 149
XmaJI CCTAGG 1 cut(s) 77
XmnI GAANNNNTTC 1 cut(s) 1313
XspI CTAG 2 cut(s) 78, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.