FvH4_2g12480

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009241
Physical Location & Seq
Forward (+)
10935490 .. 10940841
5352 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g12480.t2

Sequence Viewer

Length: 1590 bp
ATGTCGATGCTCGATTCCTTCTTCAACAAGGGCTTCAAAGCCGCCAAATGCAAAACTCTGCTGAAACTGACTATTCCGCGGATAAAGCTGCTGAGGAACAGGAGAGAAATTCAGATCAAACAGATGCGCCGTGACATTGCCAAGCTTCTTGAGACTGGCCAAGAAGCTACTGCTCGGATTCGGGTAGAGCATATTATTAGAGAAGAGAACATGATGGCAGCTCAGGAGTTAATTGAGCTATATTGTGAGCTGATTTCTGTCCGCCTTCCAATAGTTGAATCACAAAGGGAGTGTCCTCTTGACTTGAAAGAAGCAATATCCAGTTTGTGTTTTGCTGCACCAAGATGTGCTGATCTGCCAGAGTTGCTTCAAGTTCAAATGCTATTTGTATCTAAATACGGGAAAGAATTTGTATCAGCAGCTACAGAGCTAATGCCAGATTGTGGTGTCAGTCGTCAGTTGATAGAGTTGCTATCTGTTCGGGCTCCTTCCGCTGAGAAAAAGTTGAAGCTTTTGAAAGAAATTGCCGAAGAGCATGAACTGGATTGGGATCCTGCTGCATCTGAAGCTGAAATCTTCAAACCGCATGAGGATTTGCTGAATGGTCCGACACTGTTTGTCAGTGGGTCTAAGTTGCCGCTTCCCAAAGAAAAACATGATGAGACATTGTACTGTGCTCCTGATCAAGTTCAGAAAGAACAGTCTGATTCTGATGATGGTTTGGACGGATTAGACTTTCCGGAAGTTCCTAAAGTATCAATAAGACCCAATGAGAGTACAGCCTCAGCACCTGTAATGGCTCCACTTTTTCCAACCCCACCACACCCTGAAATTGATGACAAGTTATCAAAAGATTCTGAGGTGATTGGACATCCATACAAAGAACCGTCCTTTAAGTCTGAAGAAGAAAGAGCAATAGAAAACAAGCATGAATCTCATGCTTCAGTTGGTCATGGAAATGATGTACAGTTTTTGCCATTTATCTCTCCCCCATCTCTTTCTTCTGCATCGTTTCCTGTGAGACATACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTATTAGCTGCTGCTCAGGCCGCTGCAGATTCGGCAGAACGTGCGGCAGCAGCAGCTCGCTCGGCAGCTAGTCTTGCACAGGTAAGAATCAATGAGCTGACCAAGAAAAACAATGAAGAAGTATCTGAAAGTAGCAGTGAAAACCCATTTCATGAAGAGATTCCTAGTCAGTCAGCTAAAGAAAAGCCAGATTTTGATCATCTTATCCCTGATGGTAATTCTAGTGGTGTTATAAATTCTCCGGAACCCCATCAAGTCCATGAATATGGCCGGGGATCAGAGACATCAAATCTTTCATTTATGGACTCGGCTAAAGTGGATTTTGATTCCGCACCTCCTCCCAGTGATCATGTTTTTGAACACGAGCCTGCTCATCACCAGCCTCAGAGATTGACTTCAATGGATGATGACCCATACTTCTCGTACCCAAACTTATTCACATCGGGAAACAATGTTGGATCTGGTACTCACTCTTCCACAGATAATTCACATGAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

530

Amino Acids

58.66

Weight (kDa)

5.1

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 1.6e-60 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1322
AccB7I CCANNNNNTGG 1 cut(s) 443
AccII CGCG 1 cut(s) 79
AccIII TCCGGA 2 cut(s) 739, 1330
AclWI GGATC 4 cut(s) 545, 558, 1372, 1555
AcoI YGGCCR 2 cut(s) 157, 1357
AcsI RAATTY 3 cut(s) 108, 407, 1324
AcuI CTGAAG 3 cut(s) 585, 921, 927
AfaI GTAC 5 cut(s) 671, 778, 966, 1514, 1555
AfiI CCNNNNNNNGG 1 cut(s) 443
Alw21I GWGCWC 1 cut(s) 679
Alw26I GTCTC 4 cut(s) 146, 656, 1015, 1364
AlwI GGATC 4 cut(s) 545, 558, 1372, 1555
AlwNI CAGNNNCTG 1 cut(s) 791
Aor13HI TCCGGA 2 cut(s) 739, 1330
AoxI GGCC 3 cut(s) 157, 1107, 1357
ApoI RAATTY 3 cut(s) 108, 407, 1324
ArsI GACNNNNNNTTYG 2 cut(s) 277, 309
Asp700I GAANNNNTTC 1 cut(s) 1248
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 605
AsuC2I CCSGG 1 cut(s) 1361
AsuHPI GGTGA 2 cut(s) 874, 1457
AvaII GGWCC 1 cut(s) 605
BalI TGGCCA 1 cut(s) 159
BamHI GGATCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 487
BarI GAAGNNNNNNTAC 2 cut(s) 1546, 1578
BauI CACGAG 1 cut(s) 1451
Bbv12I GWGCWC 1 cut(s) 679
BbvCI CCTCAGC 2 cut(s) 92, 784
BccI CCATC 5 cut(s) 208, 710, 1000, 1295, 1347
BceAI ACGGC 1 cut(s) 114
BcgI CGANNNNNNTGC 2 cut(s) 1131, 1165
BclI TGATCA 3 cut(s) 682, 1285, 1435
BcnI CCSGG 1 cut(s) 1361
BcoDI GTCTC 4 cut(s) 146, 656, 1015, 1364
BfaI CTAG 3 cut(s) 1158, 1254, 1311
BfmI CTRYAG 2 cut(s) 423, 1113
Bme1390I CCNGG 1 cut(s) 1361
Bme18I GGWCC 1 cut(s) 605
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 4 cut(s) 486, 552, 801, 1335
BmrFI CCNGG 1 cut(s) 1361
BmrI ACTGGG 1 cut(s) 1425
BmsI GCATC 3 cut(s) 114, 569, 1016
BmuI ACTGGG 1 cut(s) 1425
Bpu10I CCTNAGC 4 cut(s) 92, 222, 784, 1104
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 1 cut(s) 1361
BsaBI GATNNNNATC 2 cut(s) 549, 1284
BsaJI CCNNGG 2 cut(s) 77, 1360
BsaWI WCCGGW 2 cut(s) 739, 1330
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse1I ACTGG 4 cut(s) 160, 321, 546, 1431
Bse3DI GCAATG 1 cut(s) 135
Bse8I GATNNNNATC 2 cut(s) 549, 1284
BseAI TCCGGA 2 cut(s) 739, 1330
BseDI CCNNGG 2 cut(s) 77, 1360
BseGI GGATG 2 cut(s) 871, 1498
BseJI GATNNNNATC 2 cut(s) 549, 1284
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 7 cut(s) 83, 236, 486, 798, 849, 1118, 1487
BseNI ACTGG 4 cut(s) 160, 321, 546, 1431
BseRI GAGGAG 1 cut(s) 1416
BsgI GTGCAG 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 79
BshFI GGCC 3 cut(s) 159, 1109, 1359
BsiHKAI GWGCWC 1 cut(s) 679
BsiSI CCGG 3 cut(s) 740, 1331, 1360
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 4 cut(s) 146, 656, 1015, 1364
BsnI GGCC 3 cut(s) 159, 1109, 1359
Bsp1286I GDGCHC 2 cut(s) 487, 679
Bsp13I TCCGGA 2 cut(s) 739, 1330
Bsp1407I TGTACA 1 cut(s) 964
Bsp143I GATC 8 cut(s) 114, 352, 550, 682, 1285, 1364, 1435, 1547
BspANI GGCC 3 cut(s) 159, 1109, 1359
BspCNI CTCAG 7 cut(s) 84, 235, 487, 797, 850, 1117, 1486
BspEI TCCGGA 2 cut(s) 739, 1330
BspFNI CGCG 1 cut(s) 79
BspHI TCATGA 1 cut(s) 1240
BspLI GGNNCC 4 cut(s) 486, 552, 801, 1335
BspMAI CTGCAG 1 cut(s) 1117
BspPI GGATC 4 cut(s) 545, 558, 1372, 1555
BspQI GCTCTTC 1 cut(s) 525
BsrDI GCAATG 1 cut(s) 135
BsrGI TGTACA 1 cut(s) 964
BsrI ACTGG 4 cut(s) 160, 321, 546, 1431
BssECI CCNNGG 2 cut(s) 77, 1360
BssMI GATC 8 cut(s) 114, 352, 550, 682, 1285, 1364, 1435, 1547
BssSI CACGAG 1 cut(s) 1451
Bst2BI CACGAG 1 cut(s) 1451
Bst4CI ACNGT 6 cut(s) 615, 674, 702, 888, 969, 1587
Bst6I CTCTTC 4 cut(s) 198, 525, 1239, 1567
BstAPI GCANNNNNTGC 1 cut(s) 1130
BstAUI TGTACA 1 cut(s) 964
BstC8I GCNNGC 2 cut(s) 1147, 1458
BstDEI CTNAG 8 cut(s) 92, 222, 495, 630, 784, 858, 1104, 1473
BstDSI CCRYGG 1 cut(s) 77
BstF5I GGATG 2 cut(s) 871, 1498
BstFNI CGCG 1 cut(s) 79
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 8 cut(s) 117, 355, 553, 685, 1288, 1367, 1438, 1550
BstMAI GTCTC 4 cut(s) 146, 656, 1015, 1364
BstMBI GATC 8 cut(s) 114, 352, 550, 682, 1285, 1364, 1435, 1547
BstSCI CCNGG 1 cut(s) 1359
BstSFI CTRYAG 2 cut(s) 423, 1113
BstUI CGCG 1 cut(s) 79
BstX2I RGATCY 2 cut(s) 550, 1547
BstXI CCANNNNNNTGG 1 cut(s) 1355
BstYI RGATCY 2 cut(s) 550, 1547
BsuRI GGCC 3 cut(s) 159, 1109, 1359
BtgI CCRYGG 1 cut(s) 77
BtsCI GGATG 2 cut(s) 871, 1498
BtsI GCAGTG 1 cut(s) 1231
BtsIMutI CAGTG 4 cut(s) 611, 628, 1231, 1438
Cac8I GCNNGC 2 cut(s) 1147, 1458
CaiI CAGNNNCTG 1 cut(s) 791
CciI TCATGA 1 cut(s) 1240
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 605
Cfr42I CCGCGG 1 cut(s) 80
Csp6I GTAC 5 cut(s) 670, 777, 965, 1513, 1554
CviQI GTAC 5 cut(s) 670, 777, 965, 1513, 1554
DdeI CTNAG 8 cut(s) 92, 222, 495, 630, 784, 858, 1104, 1473
DpnI GATC 8 cut(s) 116, 354, 552, 684, 1287, 1366, 1437, 1549
DpnII GATC 8 cut(s) 114, 352, 550, 682, 1285, 1364, 1435, 1547
EaeI YGGCCR 2 cut(s) 157, 1357
Eam1104I CTCTTC 4 cut(s) 198, 525, 1239, 1567
EarI CTCTTC 4 cut(s) 198, 525, 1239, 1567
EciI GGCGGA 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 605
Eco57I CTGAAG 3 cut(s) 585, 921, 927
EcoT38I GRGCYC 1 cut(s) 487
FbaI TGATCA 3 cut(s) 682, 1285, 1435
FokI GGATG 2 cut(s) 858, 1505
FriOI GRGCYC 1 cut(s) 487
FspBI CTAG 3 cut(s) 1158, 1254, 1311
GlaI GCGC 1 cut(s) 128
HaeIII GGCC 3 cut(s) 159, 1109, 1359
HapII CCGG 3 cut(s) 740, 1331, 1360
HhaI GCGC 1 cut(s) 129
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HindIII AAGCTT 2 cut(s) 143, 509
HpaII CCGG 3 cut(s) 740, 1331, 1360
HphI GGTGA 2 cut(s) 874, 1457
Hpy188III TCNNGA 9 cut(s) 149, 224, 299, 680, 740, 1053, 1241, 1331, 1533
HpyAV CCTTC 3 cut(s) 28, 275, 498
HpyCH4III ACNGT 6 cut(s) 615, 674, 702, 888, 969, 1587
HpyCH4IV ACGT 1 cut(s) 1129
HpyCH4V TGCA 7 cut(s) 51, 338, 560, 1007, 1084, 1115, 1166
HpyF3I CTNAG 8 cut(s) 92, 222, 495, 630, 784, 858, 1104, 1473
HpySE526I ACGT 1 cut(s) 1129
HspAI GCGC 1 cut(s) 127
Kpn2I TCCGGA 2 cut(s) 739, 1330
Ksp22I TGATCA 3 cut(s) 682, 1285, 1435
KspI CCGCGG 1 cut(s) 80
Kzo9I GATC 8 cut(s) 114, 352, 550, 682, 1285, 1364, 1435, 1547
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 3 cut(s) 490, 682, 805
LweI GCATC 3 cut(s) 114, 569, 1016
MaeI CTAG 3 cut(s) 1158, 1254, 1311
MaeII ACGT 1 cut(s) 1129
MaeIII GTNAC 1 cut(s) 131
MalI GATC 8 cut(s) 116, 354, 552, 684, 1287, 1366, 1437, 1549
MboI GATC 8 cut(s) 114, 352, 550, 682, 1285, 1364, 1435, 1547
MflI RGATCY 2 cut(s) 550, 1547
MhlI GDGCHC 2 cut(s) 487, 679
MlsI TGGCCA 1 cut(s) 159
MluCI AATT 8 cut(s) 108, 231, 407, 522, 831, 1306, 1324, 1573
MluNI TGGCCA 1 cut(s) 159
MlyI GAGTC 1 cut(s) 1388
MmeI TCCRAC 3 cut(s) 632, 836, 1525
Mox20I TGGCCA 1 cut(s) 159
MroI TCCGGA 2 cut(s) 739, 1330
MroXI GAANNNNTTC 1 cut(s) 1248
MscI TGGCCA 1 cut(s) 159
MseI TTAA 2 cut(s) 230, 894
MslI CAYNNNNRTG 3 cut(s) 343, 957, 1353
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 3 cut(s) 79, 494, 1112
MspI CCGG 3 cut(s) 740, 1331, 1360
MspR9I CCNGG 1 cut(s) 1361
MvnI CGCG 1 cut(s) 79
NciI CCSGG 1 cut(s) 1361
NdeII GATC 8 cut(s) 114, 352, 550, 682, 1285, 1364, 1435, 1547
NlaIV GGNNCC 4 cut(s) 486, 552, 801, 1335
NmeAIII GCCGAG 2 cut(s) 1130, 1376
NmuCI GTSAC 1 cut(s) 131
PagI TCATGA 1 cut(s) 1240
PciSI GCTCTTC 1 cut(s) 525
PdmI GAANNNNTTC 1 cut(s) 1248
PflMI CCANNNNNTGG 1 cut(s) 443
PleI GAGTC 1 cut(s) 1388
PpsI GAGTC 1 cut(s) 1388
PsiI TTATAA 1 cut(s) 1322
PspN4I GGNNCC 4 cut(s) 486, 552, 801, 1335
PspPI GGNCC 1 cut(s) 605
PstI CTGCAG 1 cut(s) 1117
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 2 cut(s) 550, 1547
RsaI GTAC 5 cut(s) 671, 778, 966, 1514, 1555
RsaNI GTAC 5 cut(s) 670, 777, 965, 1513, 1554
RseI CAYNNNNRTG 3 cut(s) 343, 957, 1353
SacII CCGCGG 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 2 cut(s) 230, 894
Sau3AI GATC 8 cut(s) 114, 352, 550, 682, 1285, 1364, 1435, 1547
Sau96I GGNCC 1 cut(s) 605
SchI GAGTC 1 cut(s) 1388
ScrFI CCNGG 1 cut(s) 1361
SduI GDGCHC 2 cut(s) 487, 679
SfaNI GCATC 3 cut(s) 114, 569, 1016
SfcI CTRYAG 2 cut(s) 423, 1113
Sfr303I CCGCGG 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 80
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 3 cut(s) 343, 957, 1353
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 8 cut(s) 108, 231, 407, 522, 831, 1306, 1324, 1573
SspMI CTAG 3 cut(s) 1158, 1254, 1311
StyD4I CCNGG 1 cut(s) 1359
TaaI ACNGT 6 cut(s) 615, 674, 702, 888, 969, 1587
TaiI ACGT 1 cut(s) 1132
TaqI TCGA 2 cut(s) 5, 12
TasI AATT 8 cut(s) 108, 231, 407, 522, 831, 1306, 1324, 1573
TatI WGTACW 3 cut(s) 669, 776, 964
TauI GCSGC 4 cut(s) 44, 640, 1112, 1136
Tru1I TTAA 2 cut(s) 230, 894
Tru9I TTAA 2 cut(s) 230, 894
TscAI CASTG 4 cut(s) 618, 628, 1231, 1438
TseFI GTSAC 1 cut(s) 131
Tsp45I GTSAC 1 cut(s) 131
TspDTI ATGAA 7 cut(s) 552, 945, 1218, 1229, 1257, 1365, 1374
TspGWI ACGGA 1 cut(s) 741
TspRI CASTG 4 cut(s) 618, 628, 1231, 1438
Van91I CCANNNNNTGG 1 cut(s) 443
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 3 cut(s) 108, 407, 1324
XmnI GAANNNNTTC 1 cut(s) 1248
XspI CTAG 3 cut(s) 1158, 1254, 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.