Rw6G016210

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009241
Physical Location & Seq
Forward (+)
31640578 .. 31645068
4491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G016210.1

Sequence Viewer

Length: 1716 bp
ATGTCGATGCTCGATTCCTTCTTCAACAAGGGCTTCAAAGCTGCCAAATGCAAAACTCTGCTGAAATTGACGATTCCGCGGATAAAGTTGTTGAGGAACAGGAGAGAAATTCAGCTTAGACAGATGCGCCGTGACATTGCCAAGCTTCTTGAGACTGGCCAGGAAGCTACAGCTCGGATTCGGGTGGAGCATATTATTAGAGAAGAGAATATGATGGCAGCTCAGGAGATCATCGAGCTATATGCTGAGCTTATTTCTGTCCGCCTTCCAATAGTTGAATCACAAAGGGAGTGCCCTCTAGACTTGAAAGAAGCAATATCCAGTGTGTGTTTTGCTGCACCAAGATGCGCTGATCTGCCGGAGTTGCTTCAAGTTCAAATGCTATTTTTATCAAAATATGGGAAAGAATTTGTATCAGCTGCAACAGAGCTAATGCCAGATTGTGGTGTCAGTCGCCAGTTGATAGAACTGCTATCTGTTCGGGCTCCTTCAGCTGAGAAAAAGCTGAAGCTTTTGAAAGAAATTGCTGAAGAGCATGAACTGGATTGGGATCCTGCTGCATCTGAAGCTGAAATTTTCAAACCGCATGAGGATTTGCTGAATGGTCCGACACAGTTTGTCAGCGGGTCTAAGTTGCCGCTTCCCGAAGAAAAACATGATGAGACGTTGTACCGTGCACCTGATCAAGCTCAGAAAGAACAGTCTGATTCTGATGATGGTTTGGACGTTTTAGACTTTCCGGAAGTTCCGAAGGTATCATCAATACCAACTGAGAGTATACCCTCAGCACCAACAATGGCTCCACCTTTTCCACCCCCACCACACCCTGATTTTGATCACGAGTTATCAAAAAATTCTGAGGCGATTGGACATCCATCCAAAGAGCCGTCCTTTGAGTCTGGAGAAGAAAGAGCAATGGCAGACAAGCATGAATCTCATGCTTCAGTTGGTCATGGGGATGATGTACAGTTTTTGCCATTTATATCTCCTCCATCTCTTTCATCTGCATCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTCAGCTGCTGCTCAGGCTGCTGCAGATTCTGCAGCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTTAGCTGCTGCTCAGGCTGCTGCAGATTCGGCAGAACGTGCGGCAGCAGCAGCTCGCTCAGCAGCTAGTCTTGCACAGGTAAGAATAAATGAGCTGACCAAGAAAAACAATGAAGAAGTATCTGAAAGTAGCTGTGAAAACCCATTTCATGAAGACGTTCCTAATCAGTCAGCTACTAAAGAAAAGTCGGATTTTGATCATCATATCCCCGATGGTGATTCTAGTGGTGTTATAAATTCTCCAGAACCCCATCAAGGCCATGAATATGGCCGGGGATCAGAGACATCAAATCTTTCGTTTAAGGACTCGGCTAAAGTGGATTTTGATTCCCCGCTTGCCAGCGATAATGTTTTTGAACATGAGCCAGCTCATCACCAGCCTCAGAGATTGACTTCAATGGATGATGACCCATACTTCTCGTACCCAAACTTATTCACATCAGAAAACAATGTTGGATCTGGTGCTCAGTCTTCTACAGATAATTCTCGCTTCTCGCATGAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

571

Amino Acids

62.97

Weight (kDa)

5.0

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 1.3e-58 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1442
AccB7I CCANNNNNTGG 1 cut(s) 443
AccI GTMKAC 1 cut(s) 778
AccII CGCG 1 cut(s) 79
AccIII TCCGGA 1 cut(s) 739
AciI CCGC 8 cut(s) 77, 79, 262, 584, 624, 638, 1250, 1541
AclWI GGATC 4 cut(s) 545, 558, 1492, 1672
AcoI YGGCCR 2 cut(s) 157, 1477
AcsI RAATTY 5 cut(s) 108, 407, 573, 853, 1444
AcuI CTGAAG 5 cut(s) 474, 527, 549, 585, 927
AfaI GTAC 3 cut(s) 671, 966, 1631
AfiI CCNNNNNNNGG 2 cut(s) 443, 1463
AjnI CCWGG 1 cut(s) 159
AjuI GAANNNNNNNTTGG 2 cut(s) 1644, 1676
Alw21I GWGCWC 2 cut(s) 679, 1675
Alw26I GTCTC 5 cut(s) 146, 656, 1015, 1132, 1484
Alw44I GTGCAC 1 cut(s) 675
AlwI GGATC 4 cut(s) 545, 558, 1492, 1672
AlwNI CAGNNNCTG 2 cut(s) 1100, 1121
Aor13HI TCCGGA 1 cut(s) 739
AoxI GGCC 3 cut(s) 157, 1465, 1477
ApaLI GTGCAC 1 cut(s) 675
ApoI RAATTY 5 cut(s) 108, 407, 573, 853, 1444
Asp700I GAANNNNTTC 1 cut(s) 1365
AspLEI GCGC 2 cut(s) 129, 350
AspS9I GGNCC 1 cut(s) 605
AsuC2I CCSGG 1 cut(s) 1481
AsuHPI GGTGA 2 cut(s) 1436, 1574
AvaII GGWCC 1 cut(s) 605
BaeGI GKGCMC 2 cut(s) 296, 679
BalI TGGCCA 1 cut(s) 159
BamHI GGATCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 487
BauI CACGAG 1 cut(s) 839
BbsI GAAGAC 2 cut(s) 1368, 1671
Bbv12I GWGCWC 2 cut(s) 679, 1675
BbvCI CCTCAGC 1 cut(s) 784
BccI CCATC 6 cut(s) 208, 710, 883, 1000, 1415, 1467
BceAI ACGGC 2 cut(s) 114, 871
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciT130I CCWGG 1 cut(s) 161
BclI TGATCA 3 cut(s) 682, 835, 1405
BcnI CCSGG 1 cut(s) 1481
BcoDI GTCTC 5 cut(s) 146, 656, 1015, 1132, 1484
BfaI CTAG 3 cut(s) 299, 1275, 1431
BfmI CTRYAG 5 cut(s) 168, 1113, 1122, 1230, 1683
BlpI GCTNAGC 2 cut(s) 246, 1267
Bme1390I CCNGG 2 cut(s) 161, 1481
Bme18I GGWCC 1 cut(s) 605
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 3 cut(s) 486, 552, 801
BmrFI CCNGG 2 cut(s) 161, 1481
BmsI GCATC 4 cut(s) 114, 335, 569, 1016
BpiI GAAGAC 2 cut(s) 1368, 1671
BpmI CTGGAG 2 cut(s) 921, 1434
Bpu10I CCTNAGC 4 cut(s) 222, 784, 1104, 1221
Bpu1102I GCTNAGC 2 cut(s) 246, 1267
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 1 cut(s) 1481
BsaBI GATNNNNATC 3 cut(s) 549, 834, 1404
BsaJI CCNNGG 2 cut(s) 77, 1480
BsaWI WCCGGW 1 cut(s) 739
BsaXI ACNNNNNCTCC 2 cut(s) 784, 814
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 1463
Bse1I ACTGG 4 cut(s) 160, 321, 457, 546
Bse3DI GCAATG 2 cut(s) 135, 921
Bse8I GATNNNNATC 3 cut(s) 549, 834, 1404
BseAI TCCGGA 1 cut(s) 739
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 2 cut(s) 77, 1480
BseGI GGATG 4 cut(s) 871, 875, 964, 1615
BseJI GATNNNNATC 3 cut(s) 549, 834, 1404
BseLI CCNNNNNNNGG 2 cut(s) 443, 1463
BseMI GCAATG 2 cut(s) 135, 921
BseNI ACTGG 4 cut(s) 160, 321, 457, 546
BseRI GAGGAG 1 cut(s) 978
BseSI GKGCMC 2 cut(s) 296, 679
BsgI GTGCAG 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 79
BshFI GGCC 3 cut(s) 159, 1467, 1479
BsiHKAI GWGCWC 2 cut(s) 679, 1675
BsiSI CCGG 3 cut(s) 359, 740, 1480
BslI CCNNNNNNNGG 2 cut(s) 443, 1463
BsmAI GTCTC 5 cut(s) 146, 656, 1015, 1132, 1484
BsmBI CGTCTC 1 cut(s) 656
BsnI GGCC 3 cut(s) 159, 1467, 1479
Bsp1286I GDGCHC 4 cut(s) 296, 487, 679, 1675
Bsp13I TCCGGA 1 cut(s) 739
Bsp1407I TGTACA 1 cut(s) 964
Bsp143I GATC 8 cut(s) 228, 352, 550, 682, 835, 1405, 1484, 1664
Bsp1720I GCTNAGC 2 cut(s) 246, 1267
BspACI CCGC 8 cut(s) 77, 79, 262, 584, 624, 638, 1250, 1541
BspANI GGCC 3 cut(s) 159, 1467, 1479
BspEI TCCGGA 1 cut(s) 739
BspFNI CGCG 1 cut(s) 79
BspHI TCATGA 1 cut(s) 1357
BspLI GGNNCC 3 cut(s) 486, 552, 801
BspMAI CTGCAG 3 cut(s) 1117, 1126, 1234
BspPI GGATC 4 cut(s) 545, 558, 1492, 1672
BspQI GCTCTTC 1 cut(s) 525
BsrDI GCAATG 2 cut(s) 135, 921
BsrGI TGTACA 1 cut(s) 964
BsrI ACTGG 4 cut(s) 160, 321, 457, 546
BssECI CCNNGG 2 cut(s) 77, 1480
BssMI GATC 8 cut(s) 228, 352, 550, 682, 835, 1405, 1484, 1664
BssNAI GTATAC 1 cut(s) 779
BssSI CACGAG 1 cut(s) 839
Bst1107I GTATAC 1 cut(s) 779
Bst2BI CACGAG 1 cut(s) 839
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 4 cut(s) 615, 674, 702, 969
Bst6I CTCTTC 2 cut(s) 198, 525
BstAPI GCANNNNNTGC 2 cut(s) 1121, 1247
BstAUI TGTACA 1 cut(s) 964
BstC8I GCNNGC 4 cut(s) 1264, 1545, 1549, 1575
BstDSI CCRYGG 1 cut(s) 77
BstF5I GGATG 4 cut(s) 871, 875, 964, 1615
BstFNI CGCG 1 cut(s) 79
BstHHI GCGC 2 cut(s) 129, 350
BstKTI GATC 8 cut(s) 231, 355, 553, 685, 838, 1408, 1487, 1667
BstMAI GTCTC 5 cut(s) 146, 656, 1015, 1132, 1484
BstMBI GATC 8 cut(s) 228, 352, 550, 682, 835, 1405, 1484, 1664
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 2 cut(s) 159, 1479
BstSFI CTRYAG 5 cut(s) 168, 1113, 1122, 1230, 1683
BstSLI GKGCMC 2 cut(s) 296, 679
BstUI CGCG 1 cut(s) 79
BstV2I GAAGAC 2 cut(s) 1368, 1671
BstX2I RGATCY 2 cut(s) 550, 1664
BstXI CCANNNNNNTGG 1 cut(s) 1475
BstYI RGATCY 2 cut(s) 550, 1664
BstZ17I GTATAC 1 cut(s) 779
BsuRI GGCC 3 cut(s) 159, 1467, 1479
BtgI CCRYGG 1 cut(s) 77
BtsCI GGATG 4 cut(s) 871, 875, 964, 1615
BtsIMutI CAGTG 2 cut(s) 328, 1711
Cac8I GCNNGC 4 cut(s) 1264, 1545, 1549, 1575
CaiI CAGNNNCTG 2 cut(s) 1100, 1121
CciI TCATGA 1 cut(s) 1357
CfoI GCGC 2 cut(s) 129, 350
Cfr13I GGNCC 1 cut(s) 605
Cfr42I CCGCGG 1 cut(s) 80
Csp6I GTAC 3 cut(s) 670, 965, 1630
CviQI GTAC 3 cut(s) 670, 965, 1630
DpnI GATC 8 cut(s) 230, 354, 552, 684, 837, 1407, 1486, 1666
DpnII GATC 8 cut(s) 228, 352, 550, 682, 835, 1405, 1484, 1664
EaeI YGGCCR 2 cut(s) 157, 1477
Eam1104I CTCTTC 2 cut(s) 198, 525
EarI CTCTTC 2 cut(s) 198, 525
EciI GGCGGA 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 605
Eco57I CTGAAG 5 cut(s) 474, 527, 549, 585, 927
EcoRII CCWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 487
Esp3I CGTCTC 1 cut(s) 656
FauI CCCGC 2 cut(s) 617, 1548
FbaI TGATCA 3 cut(s) 682, 835, 1405
FblI GTMKAC 1 cut(s) 778
FokI GGATG 4 cut(s) 858, 862, 971, 1622
FriOI GRGCYC 1 cut(s) 487
FspBI CTAG 3 cut(s) 299, 1275, 1431
GlaI GCGC 2 cut(s) 128, 349
GsuI CTGGAG 2 cut(s) 921, 1434
HaeIII GGCC 3 cut(s) 159, 1467, 1479
HapII CCGG 3 cut(s) 359, 740, 1480
HhaI GCGC 2 cut(s) 129, 350
Hin6I GCGC 2 cut(s) 127, 348
HinP1I GCGC 2 cut(s) 127, 348
HindIII AAGCTT 2 cut(s) 143, 509
HpaII CCGG 3 cut(s) 359, 740, 1480
HphI GGTGA 2 cut(s) 1436, 1574
Hpy166II GTNNAC 2 cut(s) 677, 779
Hpy8I GTNNAC 2 cut(s) 677, 779
HpyAV CCTTC 4 cut(s) 28, 275, 498, 745
HpyCH4III ACNGT 4 cut(s) 615, 674, 702, 969
HpyCH4IV ACGT 4 cut(s) 665, 726, 1246, 1365
HpySE526I ACGT 4 cut(s) 665, 726, 1246, 1365
HspAI GCGC 2 cut(s) 127, 348
Kpn2I TCCGGA 1 cut(s) 739
Ksp22I TGATCA 3 cut(s) 682, 835, 1405
KspI CCGCGG 1 cut(s) 80
Kzo9I GATC 8 cut(s) 228, 352, 550, 682, 835, 1405, 1484, 1664
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 3 cut(s) 187, 490, 805
LweI GCATC 4 cut(s) 114, 335, 569, 1016
MaeI CTAG 3 cut(s) 299, 1275, 1431
MaeII ACGT 4 cut(s) 665, 726, 1246, 1365
MaeIII GTNAC 1 cut(s) 131
MalI GATC 8 cut(s) 230, 354, 552, 684, 837, 1407, 1486, 1666
MboI GATC 8 cut(s) 228, 352, 550, 682, 835, 1405, 1484, 1664
MflI RGATCY 2 cut(s) 550, 1664
MhlI GDGCHC 4 cut(s) 296, 487, 679, 1675
MlsI TGGCCA 1 cut(s) 159
MluCI AATT 8 cut(s) 65, 108, 407, 522, 573, 853, 1444, 1690
MluNI TGGCCA 1 cut(s) 159
MlyI GAGTC 2 cut(s) 905, 1508
MmeI TCCRAC 3 cut(s) 632, 1377, 1642
Mox20I TGGCCA 1 cut(s) 159
MroI TCCGGA 1 cut(s) 739
MroXI GAANNNNTTC 1 cut(s) 1365
MscI TGGCCA 1 cut(s) 159
MseI TTAA 1 cut(s) 1509
MslI CAYNNNNRTG 3 cut(s) 343, 957, 1473
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 5 cut(s) 79, 419, 494, 624, 1097
MspI CCGG 3 cut(s) 359, 740, 1480
MspR9I CCNGG 2 cut(s) 161, 1481
MvaI CCWGG 1 cut(s) 161
MvnI CGCG 1 cut(s) 79
NciI CCSGG 1 cut(s) 1481
NdeII GATC 8 cut(s) 228, 352, 550, 682, 835, 1405, 1484, 1664
NlaIV GGNNCC 3 cut(s) 486, 552, 801
NmeAIII GCCGAG 1 cut(s) 1496
NmuCI GTSAC 1 cut(s) 131
PagI TCATGA 1 cut(s) 1357
PciSI GCTCTTC 1 cut(s) 525
PdmI GAANNNNTTC 1 cut(s) 1365
PflMI CCANNNNNTGG 1 cut(s) 443
PleI GAGTC 2 cut(s) 904, 1508
PpsI GAGTC 2 cut(s) 904, 1508
PsiI TTATAA 1 cut(s) 1442
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 3 cut(s) 486, 552, 801
PspPI GGNCC 1 cut(s) 605
PstI CTGCAG 3 cut(s) 1117, 1126, 1234
PstNI CAGNNNCTG 2 cut(s) 1100, 1121
PsuI RGATCY 2 cut(s) 550, 1664
PvuII CAGCTG 3 cut(s) 419, 494, 1097
RsaI GTAC 3 cut(s) 671, 966, 1631
RsaNI GTAC 3 cut(s) 670, 965, 1630
RseI CAYNNNNRTG 3 cut(s) 343, 957, 1473
SacII CCGCGG 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 1 cut(s) 1509
Sau3AI GATC 8 cut(s) 228, 352, 550, 682, 835, 1405, 1484, 1664
Sau96I GGNCC 1 cut(s) 605
SchI GAGTC 2 cut(s) 905, 1508
ScrFI CCNGG 2 cut(s) 161, 1481
SduI GDGCHC 4 cut(s) 296, 487, 679, 1675
SfaNI GCATC 4 cut(s) 114, 335, 569, 1016
SfcI CTRYAG 5 cut(s) 168, 1113, 1122, 1230, 1683
Sfr303I CCGCGG 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 80
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 3 cut(s) 343, 957, 1473
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 8 cut(s) 65, 108, 407, 522, 573, 853, 1444, 1690
SsiI CCGC 8 cut(s) 77, 79, 262, 584, 624, 638, 1250, 1541
SspMI CTAG 3 cut(s) 299, 1275, 1431
StyD4I CCNGG 2 cut(s) 159, 1479
TaaI ACNGT 4 cut(s) 615, 674, 702, 969
TaiI ACGT 4 cut(s) 668, 729, 1249, 1368
TaqI TCGA 3 cut(s) 5, 12, 234
TasI AATT 8 cut(s) 65, 108, 407, 522, 573, 853, 1444, 1690
TatI WGTACW 1 cut(s) 964
TauI GCSGC 2 cut(s) 640, 1253
Tru1I TTAA 1 cut(s) 1509
Tru9I TTAA 1 cut(s) 1509
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 1 cut(s) 131
Tsp45I GTSAC 1 cut(s) 131
TspDTI ATGAA 7 cut(s) 552, 945, 990, 1335, 1346, 1374, 1485
TspRI CASTG 1 cut(s) 328
Van91I CCANNNNNTGG 1 cut(s) 443
VneI GTGCAC 1 cut(s) 675
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 5 cut(s) 108, 407, 573, 853, 1444
XbaI TCTAGA 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 778
XmnI GAANNNNTTC 1 cut(s) 1365
XspI CTAG 3 cut(s) 299, 1275, 1431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.