pycom05g03090

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009241
Physical Location & Seq
Reverse (-)
3591186 .. 3596123
4938 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g03090.1

Sequence Viewer

Length: 1617 bp
ATGTCGATGCTCGATTCCTTCTTCAACAAGGGCTTCAAAGCGGCCAAATGCAAAACCATGCTGAAATTGACAATTCCGCGGATAAAATTGTTGAGGAACAGGAGAGAAATTCAGATTAAACAGATGCGCCGCGACATTGCCAAGCTTCTTGAGACTGGCCAAGAGGCTACTGCGAGGATTCGGGTAGAGCATATTATTAGAGAAGAGAACATGATGGCAGCTCAGGAGATCCTTGAGCTATTTTCTGAGCTTATTGCTGTCCGCCTTCCAATTATTGAATCACAAAGGGAATGCCCCATAGACTTGAAAGAAGCAATATCCAGTGTGTGTTTTGCTGCGCCAAGATGTGCTGATCTGCCAGAGTTGCTGCAAGTTCAAATGCTATTTGCTTCAAAATATGGGAAAGAATTTGTAGCAGCTGCAACAGAGCTCATACCGGATTGTGGTGTCAATCGCCAGTTGATAGAACTGCTATCTGTTCGTGCGCCCTCACCTGAGAAAAAACTGAAGCTTCTGAAAGAAATTGCTGAAGAGCATGACCTGGATTGGGATCCTGCTGCATCTGAAGCTGAAATTTACAAACCGCACGAAGATTTGTTGAGTGGTCCGACACAGTTTGTTAGTGGGTCTAAATTGCCCCTTCCCAAAGAAAAACATGATGAAACATTGCAATACAGCCCGGATCAAGACCAGAAAGAACTGTCTGATTCTGATGATGGTTTGGACTCATTAGACTTTCCTGAAGTACCCAAGCAATCACTACGACCAAGTGCAAATCCAGCCTCAGCACCTGCAATGTCTCCTCCTTTACCAACCCAGCCACACCCTGAAATTGATCATGACTTGTCAAAGAAACTTGGGTCCATTGACAGTCAATCTAAAGAGCCATCCTTTGAGTCTGGAGAAGTGATGGAAGAAAGGTCAGTAGCTAACAATCAGGAACAGTCTCATGTCTCGGTTGGTGCTGTGGAAGATAAGCAGTTTTTGCCATTTATTTCTTCCCCCTCACTTTCTTCTGCGTCATTTTCGACAAGACAAAGCAATCCACCACCAAATCTTTCAAGAACAAGAAGTGAGGTCAATGTTGACTTGCAGGATGTGTTAGCTGCTGCTCAGGCTGCTGCGAACTCTGCAGAACGTGCAGCCACAGCGGCTCGCTCGGCAGCTAGTCTTGCACAGGTCAGAATCAACGAGATTACCAAGAAAAACAGTGAAGAAGTCCCAGAAACTAGCTGTGAAAACCCATTTCATGTGGACGTTCCTAATCAGTCAGATACAAGAGAAAAACCAAACTCCAATCATCTTATCCCTGATGGTGGTTCTGCTGGTGTAATAGATTCTCCGGTACCCCATCAAATCCATTTATATGGCCAGGGATCAGAGATATCAAATGCTTCGTCTATCACTATGGAACCACTTAATGTTGATTTTGATTCCGCACATTCCAATGATCATGCTTCTGAGCATGAACCTGTTCGTCATCAGCCTCAGAGATTGGCTTCAATGGAGGATGACCCCTACTTCTCGTACCCAAACTTATTTACATCGCAAAATTCAAATGTCGGATCTGATGCTCATTCTTCTACAGATAATCCTTTCCCATCCCAAGGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

539

Amino Acids

59.29

Weight (kDa)

5.01

Isoelectric Point (pI)

58.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 6.4e-60 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 799
Acc36I ACCTGC 1 cut(s) 799
Acc65I GGTACC 1 cut(s) 1345
AccB1I GGYRCC 1 cut(s) 1345
AccII CGCG 2 cut(s) 79, 132
AciI CCGC 8 cut(s) 41, 77, 79, 130, 262, 584, 1151, 1437
AclWI GGATC 6 cut(s) 223, 545, 558, 690, 1384, 1573
AcoI YGGCCR 3 cut(s) 42, 157, 1369
AcsI RAATTY 4 cut(s) 108, 407, 573, 1552
AcuI CTGAAG 4 cut(s) 527, 549, 585, 762
AfaI GTAC 3 cut(s) 747, 1347, 1529
AfiI CCNNNNNNNGG 4 cut(s) 443, 547, 1316, 1607
AgsI TTSAA 9 cut(s) 25, 37, 278, 307, 377, 393, 1062, 1503, 1557
AjnI CCWGG 2 cut(s) 540, 1371
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 4 cut(s) 146, 804, 951, 958
AlwI GGATC 6 cut(s) 223, 545, 558, 690, 1384, 1573
AlwNI CAGNNNCTG 1 cut(s) 791
AoxI GGCC 3 cut(s) 42, 157, 1369
ApoI RAATTY 4 cut(s) 108, 407, 573, 1552
ArsI GACNNNNNNTTYG 2 cut(s) 1382, 1414
Asp700I GAANNNNTTC 1 cut(s) 1473
Asp718I GGTACC 1 cut(s) 1345
AspLEI GCGC 3 cut(s) 129, 340, 487
AspS9I GGNCC 2 cut(s) 605, 861
AsuC2I CCSGG 1 cut(s) 680
AsuHPI GGTGA 1 cut(s) 483
AvaII GGWCC 2 cut(s) 605, 861
BaeGI GKGCMC 1 cut(s) 1614
BalI TGGCCA 2 cut(s) 159, 1371
BamHI GGATCC 1 cut(s) 550
BanI GGYRCC 1 cut(s) 1345
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 432
BbvCI CCTCAGC 1 cut(s) 784
BccI CCATC 7 cut(s) 208, 710, 895, 904, 1307, 1359, 1609
BciT130I CCWGG 2 cut(s) 542, 1373
BclI TGATCA 2 cut(s) 835, 1450
BcnI CCSGG 1 cut(s) 680
BcoDI GTCTC 4 cut(s) 146, 804, 951, 958
BfaI CTAG 2 cut(s) 1167, 1230
BfmI CTRYAG 2 cut(s) 1131, 1584
BfuAI ACCTGC 1 cut(s) 799
BglI GCCNNNNNGGC 1 cut(s) 1151
Bme1390I CCNGG 3 cut(s) 542, 680, 1373
Bme18I GGWCC 2 cut(s) 605, 861
BmgT120I GGNCC 2 cut(s) 605, 861
BmiI GGNNCC 4 cut(s) 552, 862, 1347, 1413
BmrFI CCNGG 3 cut(s) 542, 680, 1373
BmsI GCATC 3 cut(s) 114, 569, 1561
BpmI CTGGAG 1 cut(s) 921
Bpu10I CCTNAGC 3 cut(s) 222, 784, 1113
BpuEI CTTGAG 2 cut(s) 170, 254
BpuMI CCSGG 1 cut(s) 680
BsaBI GATNNNNATC 1 cut(s) 549
BsaJI CCNNGG 3 cut(s) 77, 1372, 1606
BsaWI WCCGGW 2 cut(s) 436, 1342
Bsc4I CCNNNNNNNGG 4 cut(s) 443, 547, 1316, 1607
Bse1I ACTGG 3 cut(s) 160, 321, 457
Bse3DI GCAATG 3 cut(s) 135, 665, 801
Bse8I GATNNNNATC 1 cut(s) 549
BseBI CCWGG 2 cut(s) 542, 1373
BseDI CCNNGG 3 cut(s) 77, 1372, 1606
BseGI GGATG 4 cut(s) 887, 1102, 1516, 1601
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 4 cut(s) 443, 547, 1316, 1607
BseMI GCAATG 3 cut(s) 135, 665, 801
BseMII CTCAG 7 cut(s) 236, 237, 486, 798, 1127, 1452, 1502
BseNI ACTGG 3 cut(s) 160, 321, 457
BseRI GAGGAG 1 cut(s) 792
BseSI GKGCMC 1 cut(s) 1614
BseYI CCCAGC 1 cut(s) 816
BsgI GTGCAG 1 cut(s) 1161
Bsh1236I CGCG 2 cut(s) 79, 132
BshFI GGCC 3 cut(s) 44, 159, 1371
BshNI GGYRCC 1 cut(s) 1345
BsiHKAI GWGCWC 1 cut(s) 432
BsiSI CCGG 3 cut(s) 437, 680, 1343
BslFI GGGAC 1 cut(s) 1205
BslI CCNNNNNNNGG 4 cut(s) 443, 547, 1316, 1607
BsmAI GTCTC 4 cut(s) 146, 804, 951, 958
BsmFI GGGAC 1 cut(s) 1205
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 3 cut(s) 44, 159, 1371
Bsp1286I GDGCHC 2 cut(s) 432, 1614
Bsp143I GATC 8 cut(s) 228, 352, 550, 682, 835, 1376, 1450, 1565
BspACI CCGC 8 cut(s) 41, 77, 79, 130, 262, 584, 1151, 1437
BspANI GGCC 3 cut(s) 44, 159, 1371
BspCNI CTCAG 7 cut(s) 235, 238, 487, 797, 1126, 1453, 1501
BspFNI CGCG 2 cut(s) 79, 132
BspHI TCATGA 1 cut(s) 838
BspLI GGNNCC 4 cut(s) 552, 862, 1347, 1413
BspMAI CTGCAG 1 cut(s) 1135
BspMI ACCTGC 1 cut(s) 799
BspPI GGATC 6 cut(s) 223, 545, 558, 690, 1384, 1573
BspQI GCTCTTC 1 cut(s) 525
BspT107I GGYRCC 1 cut(s) 1345
BsrDI GCAATG 3 cut(s) 135, 665, 801
BsrI ACTGG 3 cut(s) 160, 321, 457
BssECI CCNNGG 3 cut(s) 77, 1372, 1606
BssMI GATC 8 cut(s) 228, 352, 550, 682, 835, 1376, 1450, 1565
BssT1I CCWWGG 1 cut(s) 1606
Bst2UI CCWGG 2 cut(s) 542, 1373
Bst4CI ACNGT 5 cut(s) 615, 702, 872, 945, 1211
Bst6I CTCTTC 2 cut(s) 198, 525
BstAPI GCANNNNNTGC 2 cut(s) 985, 1139
BstC8I GCNNGC 1 cut(s) 1156
BstDEI CTNAG 7 cut(s) 222, 246, 495, 784, 1113, 1461, 1488
BstDSI CCRYGG 1 cut(s) 77
BstF5I GGATG 4 cut(s) 887, 1102, 1516, 1601
BstFNI CGCG 2 cut(s) 79, 132
BstHHI GCGC 3 cut(s) 129, 340, 487
BstKTI GATC 8 cut(s) 231, 355, 553, 685, 838, 1379, 1453, 1568
BstMAI GTCTC 4 cut(s) 146, 804, 951, 958
BstMBI GATC 8 cut(s) 228, 352, 550, 682, 835, 1376, 1450, 1565
BstNI CCWGG 2 cut(s) 542, 1373
BstSCI CCNGG 3 cut(s) 540, 678, 1371
BstSFI CTRYAG 2 cut(s) 1131, 1584
BstSLI GKGCMC 1 cut(s) 1614
BstUI CGCG 2 cut(s) 79, 132
BstX2I RGATCY 3 cut(s) 228, 550, 1565
BstXI CCANNNNNNTGG 1 cut(s) 1367
BstYI RGATCY 3 cut(s) 228, 550, 1565
BsuRI GGCC 3 cut(s) 44, 159, 1371
BtgI CCRYGG 1 cut(s) 77
BtgZI GCGATG 1 cut(s) 1530
BtsCI GGATG 4 cut(s) 887, 1102, 1516, 1601
BtsIMutI CAGTG 3 cut(s) 328, 1216, 1612
BveI ACCTGC 1 cut(s) 799
Cac8I GCNNGC 1 cut(s) 1156
CaiI CAGNNNCTG 1 cut(s) 791
CciI TCATGA 1 cut(s) 838
CfoI GCGC 3 cut(s) 129, 340, 487
Cfr13I GGNCC 2 cut(s) 605, 861
Cfr42I CCGCGG 1 cut(s) 80
CseI GACGC 1 cut(s) 1008
Csp6I GTAC 3 cut(s) 746, 1346, 1528
CviAII CATG 9 cut(s) 58, 211, 536, 656, 839, 950, 1250, 1454, 1466
CviQI GTAC 3 cut(s) 746, 1346, 1528
DdeI CTNAG 7 cut(s) 222, 246, 495, 784, 1113, 1461, 1488
DpnI GATC 8 cut(s) 230, 354, 552, 684, 837, 1378, 1452, 1567
DpnII GATC 8 cut(s) 228, 352, 550, 682, 835, 1376, 1450, 1565
EaeI YGGCCR 3 cut(s) 42, 157, 1369
Eam1104I CTCTTC 2 cut(s) 198, 525
EarI CTCTTC 2 cut(s) 198, 525
EciI GGCGGA 1 cut(s) 251
Ecl136II GAGCTC 1 cut(s) 430
Eco130I CCWWGG 1 cut(s) 1606
Eco24I GRGCYC 1 cut(s) 432
Eco32I GATATC 1 cut(s) 1386
Eco47I GGWCC 2 cut(s) 605, 861
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 4 cut(s) 527, 549, 585, 762
EcoICRI GAGCTC 1 cut(s) 430
EcoRII CCWGG 2 cut(s) 540, 1371
EcoRV GATATC 1 cut(s) 1386
EcoT14I CCWWGG 1 cut(s) 1606
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 1606
FaeI CATG 9 cut(s) 61, 214, 539, 659, 842, 953, 1253, 1457, 1469
FaqI GGGAC 1 cut(s) 1205
FatI CATG 9 cut(s) 57, 210, 535, 655, 838, 949, 1249, 1453, 1465
FbaI TGATCA 2 cut(s) 835, 1450
FokI GGATG 4 cut(s) 874, 1109, 1523, 1588
FriOI GRGCYC 1 cut(s) 432
FspBI CTAG 2 cut(s) 1167, 1230
GlaI GCGC 3 cut(s) 128, 339, 486
GsaI CCCAGC 1 cut(s) 820
GsuI CTGGAG 1 cut(s) 921
HaeIII GGCC 3 cut(s) 44, 159, 1371
HapII CCGG 3 cut(s) 437, 680, 1343
HgaI GACGC 1 cut(s) 1008
HhaI GCGC 3 cut(s) 129, 340, 487
Hin1II CATG 9 cut(s) 61, 214, 539, 659, 842, 953, 1253, 1457, 1469
Hin6I GCGC 3 cut(s) 127, 338, 485
HinP1I GCGC 3 cut(s) 127, 338, 485
HincII GTYRAC 1 cut(s) 1087
HindII GTYRAC 1 cut(s) 1087
HindIII AAGCTT 2 cut(s) 143, 509
HinfI GANTC 9 cut(s) 14, 178, 278, 707, 725, 896, 1185, 1337, 1433
HpaII CCGG 3 cut(s) 437, 680, 1343
HphI GGTGA 1 cut(s) 483
Hpy166II GTNNAC 2 cut(s) 1087, 1255
Hpy188III TCNNGA 8 cut(s) 149, 224, 686, 740, 839, 900, 938, 1062
Hpy8I GTNNAC 2 cut(s) 1087, 1255
HpyAV CCTTC 3 cut(s) 28, 275, 650
HpyCH4III ACNGT 5 cut(s) 615, 702, 872, 945, 1211
HpyCH4IV ACGT 2 cut(s) 1138, 1257
HpyF3I CTNAG 7 cut(s) 222, 246, 495, 784, 1113, 1461, 1488
HpySE526I ACGT 2 cut(s) 1138, 1257
Hsp92II CATG 9 cut(s) 61, 214, 539, 659, 842, 953, 1253, 1457, 1469
HspAI GCGC 3 cut(s) 127, 338, 485
KpnI GGTACC 1 cut(s) 1349
Ksp22I TGATCA 2 cut(s) 835, 1450
KspI CCGCGG 1 cut(s) 80
Kzo9I GATC 8 cut(s) 228, 352, 550, 682, 835, 1376, 1450, 1565
LguI GCTCTTC 1 cut(s) 525
LweI GCATC 3 cut(s) 114, 569, 1561
MaeI CTAG 2 cut(s) 1167, 1230
MaeII ACGT 2 cut(s) 1138, 1257
MalI GATC 8 cut(s) 230, 354, 552, 684, 837, 1378, 1452, 1567
MboI GATC 8 cut(s) 228, 352, 550, 682, 835, 1376, 1450, 1565
MflI RGATCY 3 cut(s) 228, 550, 1565
MhlI GDGCHC 2 cut(s) 432, 1614
MlsI TGGCCA 2 cut(s) 159, 1371
MluNI TGGCCA 2 cut(s) 159, 1371
MlyI GAGTC 2 cut(s) 719, 905
MmeI TCCRAC 2 cut(s) 632, 1543
Mox20I TGGCCA 2 cut(s) 159, 1371
MroXI GAANNNNTTC 1 cut(s) 1473
MscI TGGCCA 2 cut(s) 159, 1371
MseI TTAA 2 cut(s) 117, 1419
MslI CAYNNNNRTG 2 cut(s) 1365, 1446
Msp20I TGGCCA 2 cut(s) 159, 1371
MspA1I CMGCKG 3 cut(s) 79, 419, 1151
MspI CCGG 3 cut(s) 437, 680, 1343
MspR9I CCNGG 3 cut(s) 542, 680, 1373
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 2 cut(s) 542, 1373
MvnI CGCG 2 cut(s) 79, 132
NciI CCSGG 1 cut(s) 680
NdeII GATC 8 cut(s) 228, 352, 550, 682, 835, 1376, 1450, 1565
NlaIII CATG 9 cut(s) 61, 214, 539, 659, 842, 953, 1253, 1457, 1469
NlaIV GGNNCC 4 cut(s) 552, 862, 1347, 1413
NmeAIII GCCGAG 1 cut(s) 1139
PagI TCATGA 1 cut(s) 838
PaqCI CACCTGC 1 cut(s) 799
PciSI GCTCTTC 1 cut(s) 525
PctI GAATGC 1 cut(s) 296
PdmI GAANNNNTTC 1 cut(s) 1473
PfeI GAWTC 7 cut(s) 14, 178, 278, 707, 1185, 1337, 1433
PleI GAGTC 2 cut(s) 719, 904
PpsI GAGTC 2 cut(s) 719, 904
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 540, 1371
PspFI CCCAGC 1 cut(s) 816
PspGI CCWGG 2 cut(s) 540, 1371
PspN4I GGNNCC 4 cut(s) 552, 862, 1347, 1413
PspPI GGNCC 2 cut(s) 605, 861
PstI CTGCAG 1 cut(s) 1135
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 3 cut(s) 228, 550, 1565
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 3 cut(s) 747, 1347, 1529
RsaNI GTAC 3 cut(s) 746, 1346, 1528
RseI CAYNNNNRTG 2 cut(s) 1365, 1446
SacI GAGCTC 1 cut(s) 432
SacII CCGCGG 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 2 cut(s) 117, 1419
Sau3AI GATC 8 cut(s) 228, 352, 550, 682, 835, 1376, 1450, 1565
Sau96I GGNCC 2 cut(s) 605, 861
SchI GAGTC 2 cut(s) 719, 905
ScrFI CCNGG 3 cut(s) 542, 680, 1373
SduI GDGCHC 2 cut(s) 432, 1614
SfaNI GCATC 3 cut(s) 114, 569, 1561
SfcI CTRYAG 2 cut(s) 1131, 1584
Sfr303I CCGCGG 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 80
SinI GGWCC 2 cut(s) 605, 861
SmiMI CAYNNNNRTG 2 cut(s) 1365, 1446
SmlI CTYRAG 2 cut(s) 149, 233
SmoI CTYRAG 2 cut(s) 149, 233
SsiI CCGC 8 cut(s) 41, 77, 79, 130, 262, 584, 1151, 1437
SspMI CTAG 2 cut(s) 1167, 1230
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 3 cut(s) 540, 678, 1371
StyI CCWWGG 1 cut(s) 1606
TaaI ACNGT 5 cut(s) 615, 702, 872, 945, 1211
TaiI ACGT 2 cut(s) 1141, 1260
TaqI TCGA 3 cut(s) 5, 12, 1028
TauI GCSGC 3 cut(s) 44, 132, 1154
TfiI GAWTC 7 cut(s) 14, 178, 278, 707, 1185, 1337, 1433
Tru1I TTAA 2 cut(s) 117, 1419
Tru9I TTAA 2 cut(s) 117, 1419
TscAI CASTG 2 cut(s) 328, 1216
TspDTI ATGAA 3 cut(s) 675, 1238, 1482
TspRI CASTG 2 cut(s) 328, 1216
VpaK11BI GGWCC 2 cut(s) 605, 861
XapI RAATTY 4 cut(s) 108, 407, 573, 1552
XmnI GAANNNNTTC 1 cut(s) 1473
XspI CTAG 2 cut(s) 1167, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.