Rorug06G0071500

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009241
Physical Location & Seq
Reverse (-)
9529834 .. 9532122
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0071500.1

Sequence Viewer

Length: 1176 bp
ATGTCTGACCTTAACTTTCACCTCCTCCTCCTCCTCCTTCAATTTTCTCTCTTCTTCACCAACCATGTCCAAGCCCTCACTTCACCTTCAGACATCTCAGCTCTCAAGGCCTTCAAAGCCTCAATCAAACCCTCCTCAATTCCCTCCTGGTCTTGCCTAGCCTCATGGGACTTCACCACCGACCCATGCACCCTCCCCCACCGCATCCACTTCACTTGCGGCGTCACCTGCTCACCCGACTCCACCCGGGTCACCCAGATCACACTCGACCCCGCCGGCTACTCCGGCACACTCACTCCACAAATTTCTCAGCTCACTCATCTCTCCACTCTCGACCTCTCCGATAACTCCTTCTTCGGCCCAATCCCGTCCTCCATTTCCTCCCTCTTCTACCTCCAAACCTTGACTCTCCGATCCAACTCATTCTCCGGCTCCCTCACTCCATCCATCACCAAGCTCAAGTCTTTAGAATCCCTAGACATTTCCCACAATTCCTTAACAGGGTTTCTCCCAAACTCCTTGAGTTCTTTATCCAACTTGAGAAGACTCGATCTCAGCTACAACAAGATCACCGGATCACTCCCAAGACTTCCACCGAAACTACTAGAACTCGCTTTGAAGCGCAACGCGCTATCTGGGCCCTTATCGAAATCGTCTTTCGACGGGTTGACTCAGTTGGAGGTGGTCGAGCTCAGCGAGAACTCGCTCTCCGGGACCTTACAGTCGTGGTTCTTTCTCATCCCTTCACTGCAACAAGTTGACCTAGCCAATAACCGCCTGACAGGTGTCGAGATCGCGAGGCCCAGCTCCGGCAGAGGTGGCGAGCTCGTGGCCGTCGACTTGGGCTTCAACAGAATCGAAGGCTACGCGCCCGCGAATTTCGCGGCATTTCCGAGACTGTCGTCGCTGTCGGTTCGTTACAACAGGCTACGTGGCAAGATCCCAGTGGAGTACGGGAAGAACAAGTCGTTGAAGCGGTTGTACTTGGACGGTAACTTTCTAAACGGACAGCCGCCGCCGGCGTTGTTAACCGGAAGTTCAGGAATCTCCGGCAGCTTGGGGGATAATTGTCTACAGGGGTGTCCAGCTTCAGCTCAGCTGTGCCTTCCGTCGCAGAAATCAAACGCTATATGCAAGCAAGCCTATGGTGGAGGAAGAGGAAGACCAAGGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

391

Amino Acids

42.16

Weight (kDa)

9.28

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 98 - 306 6.5e-10 Leucine-rich repeat region
LRR_8 PF13855 132 - 190 2e-10 Leucine rich repeat
LRR_4 PF12799 154 - 191 1.6e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 199 - 260 1.1e-07 Leucine rich repeat
LRR_8 PF13855 276 - 332 9.6e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
AasI GACNNNNNNGTC 1 cut(s) 721
Acc36I ACCTGC 1 cut(s) 236
AccI GTMKAC 2 cut(s) 837, 1072
AccII CGCG 5 cut(s) 629, 797, 869, 875, 884
AciI CCGC 9 cut(s) 202, 219, 273, 775, 873, 884, 976, 1013, 1016
AclWI GGATC 3 cut(s) 408, 583, 934
AcoI YGGCCR 1 cut(s) 831
AcsI RAATTY 2 cut(s) 303, 877
AcuI CTGAAG 2 cut(s) 72, 1074
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 2 cut(s) 953, 983
AfiI CCNNNNNNNGG 2 cut(s) 501, 809
AgsI TTSAA 5 cut(s) 41, 115, 619, 850, 973
AjnI CCWGG 1 cut(s) 146
AloI GAACNNNNNNTCC 2 cut(s) 692, 724
Alw21I GWGCWC 2 cut(s) 693, 828
Alw26I GTCTC 1 cut(s) 889
AlwI GGATC 3 cut(s) 408, 583, 934
Ama87I CYCGRG 1 cut(s) 246
AoxI GGCC 5 cut(s) 108, 358, 638, 800, 831
ApaI GGGCCC 1 cut(s) 642
ApeKI GCWGC 1 cut(s) 1053
ApoI RAATTY 2 cut(s) 303, 877
AspLEI GCGC 3 cut(s) 624, 631, 871
AspS9I GGNCC 5 cut(s) 359, 638, 639, 714, 801
AsuC2I CCSGG 3 cut(s) 247, 248, 712
AsuHPI GGTGA 9 cut(s) 11, 49, 75, 166, 217, 225, 244, 442, 562
AvaI CYCGRG 1 cut(s) 246
AvaII GGWCC 1 cut(s) 714
BaeGI GKGCMC 1 cut(s) 642
BanII GRGCYC 3 cut(s) 642, 693, 828
BarI GAAGNNNNNNTAC 2 cut(s) 965, 997
BauI CACGAG 1 cut(s) 827
BbsI GAAGAC 2 cut(s) 550, 1168
Bbv12I GWGCWC 2 cut(s) 693, 828
BbvI GCAGC 1 cut(s) 1065
BccI CCATC 2 cut(s) 451, 455
BceAI ACGGC 1 cut(s) 818
BciT130I CCWGG 1 cut(s) 148
BcnI CCSGG 3 cut(s) 247, 248, 712
BcoDI GTCTC 1 cut(s) 889
BfaI CTAG 4 cut(s) 158, 476, 605, 764
BfmI CTRYAG 1 cut(s) 1073
BfuAI ACCTGC 1 cut(s) 236
BisI GCNGC 5 cut(s) 220, 885, 1013, 1016, 1054
BlpI GCTNAGC 2 cut(s) 692, 1095
BlsI GCNGC 5 cut(s) 221, 886, 1014, 1017, 1055
Bme1390I CCNGG 4 cut(s) 148, 247, 248, 712
Bme18I GGWCC 1 cut(s) 714
BmeT110I CYCGRG 1 cut(s) 246
BmgT120I GGNCC 5 cut(s) 359, 638, 639, 714, 801
BmiI GGNNCC 3 cut(s) 433, 640, 715
BmrFI CCNGG 4 cut(s) 148, 247, 248, 712
BmrI ACTGGG 1 cut(s) 938
BmsI GCATC 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 938
BoxI GACNNNNGTC 3 cut(s) 785, 901, 1168
BpiI GAAGAC 2 cut(s) 550, 1168
Bpu1102I GCTNAGC 2 cut(s) 692, 1095
BpuEI CTTGAG 4 cut(s) 89, 443, 541, 559
BpuMI CCSGG 3 cut(s) 247, 248, 712
BsaAI YACGTR 1 cut(s) 932
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 246, 1166
BsaWI WCCGGW 2 cut(s) 572, 1031
BsaXI ACNNNNNCTCC 6 cut(s) 280, 310, 410, 440, 692, 722
Bsc4I CCNNNNNNNGG 2 cut(s) 501, 809
Bse118I RCCGGY 2 cut(s) 275, 1018
Bse1I ACTGG 1 cut(s) 944
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 2 cut(s) 246, 1166
BseGI GGATG 3 cut(s) 204, 443, 738
BseLI CCNNNNNNNGG 2 cut(s) 501, 809
BseMII CTCAG 6 cut(s) 111, 323, 568, 686, 706, 1109
BseNI ACTGG 1 cut(s) 944
BseRI GAGGAG 5 cut(s) 14, 17, 20, 23, 124
BseSI GKGCMC 1 cut(s) 642
BseXI GCAGC 1 cut(s) 1065
BseYI CCCAGC 1 cut(s) 803
Bsh1236I CGCG 5 cut(s) 629, 797, 869, 875, 884
BshFI GGCC 5 cut(s) 110, 360, 640, 802, 833
BsiHKAI GWGCWC 2 cut(s) 693, 828
BsiHKCI CYCGRG 1 cut(s) 246
BslFI GGGAC 2 cut(s) 182, 727
BslI CCNNNNNNNGG 2 cut(s) 501, 809
BsmAI GTCTC 1 cut(s) 889
BsmFI GGGAC 2 cut(s) 182, 727
BsnI GGCC 5 cut(s) 110, 360, 640, 802, 833
BsoBI CYCGRG 1 cut(s) 246
Bsp120I GGGCCC 1 cut(s) 638
Bsp1286I GDGCHC 3 cut(s) 642, 693, 828
Bsp143I GATC 7 cut(s) 258, 413, 550, 567, 575, 792, 939
Bsp1720I GCTNAGC 2 cut(s) 692, 1095
Bsp68I TCGCGA 1 cut(s) 797
BspACI CCGC 9 cut(s) 202, 219, 273, 775, 873, 884, 976, 1013, 1016
BspANI GGCC 5 cut(s) 110, 360, 640, 802, 833
BspCNI CTCAG 6 cut(s) 110, 322, 567, 685, 705, 1108
BspFNI CGCG 5 cut(s) 629, 797, 869, 875, 884
BspLI GGNNCC 3 cut(s) 433, 640, 715
BspMI ACCTGC 1 cut(s) 236
BspPI GGATC 3 cut(s) 408, 583, 934
BsrFI RCCGGY 2 cut(s) 275, 1018
BsrI ACTGG 1 cut(s) 944
BssAI RCCGGY 2 cut(s) 275, 1018
BssECI CCNNGG 2 cut(s) 246, 1166
BssMI GATC 7 cut(s) 258, 413, 550, 567, 575, 792, 939
BssNI GRCGYC 1 cut(s) 222
BssSI CACGAG 1 cut(s) 827
BssT1I CCWWGG 1 cut(s) 1166
Bst2BI CACGAG 1 cut(s) 827
Bst2UI CCWGG 1 cut(s) 148
Bst4CI ACNGT 3 cut(s) 723, 900, 992
Bst6I CTCTTC 3 cut(s) 56, 392, 1150
BstACI GRCGYC 1 cut(s) 222
BstBAI YACGTR 1 cut(s) 932
BstC8I GCNNGC 6 cut(s) 277, 824, 873, 1020, 1136, 1140
BstDEI CTNAG 6 cut(s) 97, 309, 554, 672, 692, 1095
BstEII GGTNACC 1 cut(s) 250
BstF5I GGATG 3 cut(s) 204, 443, 738
BstFNI CGCG 5 cut(s) 629, 797, 869, 875, 884
BstHHI GCGC 3 cut(s) 624, 631, 871
BstKTI GATC 7 cut(s) 261, 416, 553, 570, 578, 795, 942
BstMAI GTCTC 1 cut(s) 889
BstMBI GATC 7 cut(s) 258, 413, 550, 567, 575, 792, 939
BstMWI GCNNNNNNNGC 8 cut(s) 107, 116, 228, 285, 628, 637, 819, 881
BstNI CCWGG 1 cut(s) 148
BstPAI GACNNNNGTC 3 cut(s) 785, 901, 1168
BstPI GGTNACC 1 cut(s) 250
BstSCI CCNGG 4 cut(s) 146, 245, 246, 710
BstSFI CTRYAG 1 cut(s) 1073
BstSLI GKGCMC 1 cut(s) 642
BstUI CGCG 5 cut(s) 629, 797, 869, 875, 884
BstV1I GCAGC 1 cut(s) 1065
BstV2I GAAGAC 2 cut(s) 550, 1168
BstX2I RGATCY 1 cut(s) 939
BstYI RGATCY 1 cut(s) 939
BsuRI GGCC 5 cut(s) 110, 360, 640, 802, 833
BtsCI GGATG 3 cut(s) 204, 443, 738
BtsI GCAGTG 1 cut(s) 746
BtsIMutI CAGTG 2 cut(s) 746, 951
BtuMI TCGCGA 1 cut(s) 797
BveI ACCTGC 1 cut(s) 236
Cac8I GCNNGC 6 cut(s) 277, 824, 873, 1020, 1136, 1140
CfoI GCGC 3 cut(s) 624, 631, 871
Cfr10I RCCGGY 2 cut(s) 275, 1018
Cfr13I GGNCC 5 cut(s) 359, 638, 639, 714, 801
Cfr9I CCCGGG 1 cut(s) 246
CseI GACGC 1 cut(s) 211
Csp6I GTAC 2 cut(s) 952, 982
CspCI CAANNNNNGTGG 2 cut(s) 197, 232
CviAII CATG 3 cut(s) 65, 165, 186
CviQI GTAC 2 cut(s) 952, 982
DdeI CTNAG 6 cut(s) 97, 309, 554, 672, 692, 1095
DpnI GATC 7 cut(s) 260, 415, 552, 569, 577, 794, 941
DpnII GATC 7 cut(s) 258, 413, 550, 567, 575, 792, 939
DrdI GACNNNNNNGTC 1 cut(s) 721
DseDI GACNNNNNNGTC 1 cut(s) 721
EaeI YGGCCR 1 cut(s) 831
Eam1104I CTCTTC 3 cut(s) 56, 392, 1150
EarI CTCTTC 3 cut(s) 56, 392, 1150
Ecl136II GAGCTC 2 cut(s) 691, 826
Eco130I CCWWGG 1 cut(s) 1166
Eco147I AGGCCT 1 cut(s) 110
Eco24I GRGCYC 3 cut(s) 642, 693, 828
Eco47I GGWCC 1 cut(s) 714
Eco53kI GAGCTC 2 cut(s) 691, 826
Eco57I CTGAAG 2 cut(s) 72, 1074
Eco88I CYCGRG 1 cut(s) 246
Eco91I GGTNACC 1 cut(s) 250
EcoICRI GAGCTC 2 cut(s) 691, 826
EcoO109I RGGNCCY 2 cut(s) 639, 714
EcoO65I GGTNACC 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 1166
EcoT38I GRGCYC 3 cut(s) 642, 693, 828
ErhI CCWWGG 1 cut(s) 1166
FaeI CATG 3 cut(s) 68, 168, 189
FaiI YATR 6 cut(s) 66, 166, 187, 1130, 1132, 1146
FaqI GGGAC 2 cut(s) 182, 727
FatI CATG 3 cut(s) 64, 164, 185
FauI CCCGC 2 cut(s) 280, 880
FblI GTMKAC 2 cut(s) 837, 1072
Fnu4HI GCNGC 5 cut(s) 220, 885, 1013, 1016, 1054
FokI GGATG 3 cut(s) 191, 430, 725
FriOI GRGCYC 3 cut(s) 642, 693, 828
Fsp4HI GCNGC 5 cut(s) 220, 885, 1013, 1016, 1054
FspBI CTAG 4 cut(s) 158, 476, 605, 764
GlaI GCGC 3 cut(s) 623, 630, 870
GluI GCNGC 5 cut(s) 220, 885, 1013, 1016, 1054
GsaI CCCAGC 1 cut(s) 807
HaeIII GGCC 5 cut(s) 110, 360, 640, 802, 833
HgaI GACGC 1 cut(s) 211
HhaI GCGC 3 cut(s) 624, 631, 871
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 3 cut(s) 68, 168, 189
Hin6I GCGC 3 cut(s) 622, 629, 869
HinP1I GCGC 3 cut(s) 622, 629, 869
HincII GTYRAC 4 cut(s) 669, 760, 838, 1029
HindII GTYRAC 4 cut(s) 669, 760, 838, 1029
HinfI GANTC 7 cut(s) 239, 406, 470, 546, 670, 855, 1044
HpaI GTTAAC 1 cut(s) 1029
HphI GGTGA 9 cut(s) 11, 49, 75, 166, 217, 225, 244, 442, 562
Hpy166II GTNNAC 5 cut(s) 669, 760, 838, 1029, 1073
Hpy188I TCNGA 5 cut(s) 7, 91, 343, 413, 894
Hpy188III TCNNGA 5 cut(s) 332, 790, 796, 1041, 1173
Hpy8I GTNNAC 5 cut(s) 669, 760, 838, 1029, 1073
Hpy99I CGWCG 4 cut(s) 665, 839, 907, 1114
HpyAV CCTTC 7 cut(s) 47, 96, 121, 361, 753, 854, 1115
HpyCH4III ACNGT 3 cut(s) 723, 900, 992
HpyCH4IV ACGT 1 cut(s) 931
HpyCH4V TGCA 3 cut(s) 189, 751, 1134
HpyF10VI GCNNNNNNNGC 8 cut(s) 107, 116, 228, 285, 628, 637, 819, 881
HpyF3I CTNAG 6 cut(s) 97, 309, 554, 672, 692, 1095
HpySE526I ACGT 1 cut(s) 931
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 3 cut(s) 68, 168, 189
HspAI GCGC 3 cut(s) 622, 629, 869
KroI GCCGGC 2 cut(s) 275, 1018
KroNI GCCGGC 2 cut(s) 277, 1020
KspAI GTTAAC 1 cut(s) 1029
Kzo9I GATC 7 cut(s) 258, 413, 550, 567, 575, 792, 939
LmnI GCTCC 2 cut(s) 437, 812
Lsp1109I GCAGC 1 cut(s) 1065
LweI GCATC 1 cut(s) 213
MaeI CTAG 4 cut(s) 158, 476, 605, 764
MaeII ACGT 1 cut(s) 931
MaeIII GTNAC 4 cut(s) 223, 250, 917, 992
MalI GATC 7 cut(s) 260, 415, 552, 569, 577, 794, 941
MboI GATC 7 cut(s) 258, 413, 550, 567, 575, 792, 939
MboII GAAGA 8 cut(s) 43, 46, 346, 379, 555, 970, 1167, 1173
MflI RGATCY 1 cut(s) 939
MhlI GDGCHC 3 cut(s) 642, 693, 828
MluCI AATT 6 cut(s) 41, 138, 303, 490, 877, 1066
MlyI GAGTC 4 cut(s) 233, 400, 540, 664
MmeI TCCRAC 3 cut(s) 441, 558, 657
MreI CGCCGGCG 1 cut(s) 1018
MroNI GCCGGC 2 cut(s) 275, 1018
MseI TTAA 3 cut(s) 12, 497, 1028
MspA1I CMGCKG 1 cut(s) 1099
MspR9I CCNGG 4 cut(s) 148, 247, 248, 712
MvaI CCWGG 1 cut(s) 148
MvnI CGCG 5 cut(s) 629, 797, 869, 875, 884
MwoI GCNNNNNNNGC 8 cut(s) 107, 116, 228, 285, 628, 637, 819, 881
NaeI GCCGGC 2 cut(s) 277, 1020
NciI CCSGG 3 cut(s) 247, 248, 712
NdeII GATC 7 cut(s) 258, 413, 550, 567, 575, 792, 939
NgoMIV GCCGGC 2 cut(s) 275, 1018
NlaIII CATG 3 cut(s) 68, 168, 189
NlaIV GGNNCC 3 cut(s) 433, 640, 715
NmuCI GTSAC 2 cut(s) 223, 250
NruI TCGCGA 1 cut(s) 797
PaqCI CACCTGC 1 cut(s) 236
PceI AGGCCT 1 cut(s) 110
PcsI WCGNNNNNNNCGW 3 cut(s) 339, 693, 834
PdiI GCCGGC 2 cut(s) 277, 1020
PfeI GAWTC 3 cut(s) 470, 855, 1044
PfoI TCCNGGA 1 cut(s) 710
PkrI GCNGC 5 cut(s) 221, 886, 1014, 1017, 1055
PleI GAGTC 4 cut(s) 233, 400, 540, 664
PpsI GAGTC 4 cut(s) 233, 400, 540, 664
Ppu21I YACGTR 1 cut(s) 932
PpuMI RGGWCCY 1 cut(s) 714
PshAI GACNNNNGTC 3 cut(s) 785, 901, 1168
Psp124BI GAGCTC 2 cut(s) 693, 828
Psp5II RGGWCCY 1 cut(s) 714
Psp6I CCWGG 1 cut(s) 146
PspEI GGTNACC 1 cut(s) 250
PspFI CCCAGC 1 cut(s) 803
PspGI CCWGG 1 cut(s) 146
PspN4I GGNNCC 3 cut(s) 433, 640, 715
PspOMI GGGCCC 1 cut(s) 638
PspPI GGNCC 5 cut(s) 359, 638, 639, 714, 801
PspPPI RGGWCCY 1 cut(s) 714
PsuI RGATCY 1 cut(s) 939
PvuII CAGCTG 1 cut(s) 1099
RruI TCGCGA 1 cut(s) 797
RsaI GTAC 2 cut(s) 953, 983
RsaNI GTAC 2 cut(s) 952, 982
SacI GAGCTC 2 cut(s) 693, 828
SalI GTCGAC 1 cut(s) 836
SaqAI TTAA 3 cut(s) 12, 497, 1028
SatI GCNGC 5 cut(s) 220, 885, 1013, 1016, 1054
Sau3AI GATC 7 cut(s) 258, 413, 550, 567, 575, 792, 939
Sau96I GGNCC 5 cut(s) 359, 638, 639, 714, 801
SchI GAGTC 4 cut(s) 233, 400, 540, 664
ScrFI CCNGG 4 cut(s) 148, 247, 248, 712
SduI GDGCHC 3 cut(s) 642, 693, 828
SfaNI GCATC 1 cut(s) 213
SfcI CTRYAG 1 cut(s) 1073
SgrAI CRCCGGYG 1 cut(s) 1018
SinI GGWCC 1 cut(s) 714
SmaI CCCGGG 1 cut(s) 248
SmlI CTYRAG 4 cut(s) 104, 458, 520, 538
SmoI CTYRAG 4 cut(s) 104, 458, 520, 538
Sse9I AATT 6 cut(s) 41, 138, 303, 490, 877, 1066
SseBI AGGCCT 1 cut(s) 110
SsiI CCGC 9 cut(s) 202, 219, 273, 775, 873, 884, 976, 1013, 1016
SspMI CTAG 4 cut(s) 158, 476, 605, 764
SstI GAGCTC 2 cut(s) 693, 828
StuI AGGCCT 1 cut(s) 110
StyD4I CCNGG 4 cut(s) 146, 245, 246, 710
StyI CCWWGG 1 cut(s) 1166
TaaI ACNGT 3 cut(s) 723, 900, 992
TaiI ACGT 1 cut(s) 934
TaqI TCGA 9 cut(s) 267, 333, 549, 647, 660, 687, 789, 837, 858
TasI AATT 6 cut(s) 41, 138, 303, 490, 877, 1066
TatI WGTACW 1 cut(s) 981
TauI GCSGC 4 cut(s) 222, 887, 1015, 1018
TfiI GAWTC 3 cut(s) 470, 855, 1044
Tru1I TTAA 3 cut(s) 12, 497, 1028
Tru9I TTAA 3 cut(s) 12, 497, 1028
TscAI CASTG 2 cut(s) 753, 951
TseFI GTSAC 2 cut(s) 223, 250
TseI GCWGC 1 cut(s) 1053
Tsp45I GTSAC 2 cut(s) 223, 250
TspGWI ACGGA 2 cut(s) 1020, 1098
TspMI CCCGGG 1 cut(s) 246
TspRI CASTG 2 cut(s) 753, 951
VpaK11BI GGWCC 1 cut(s) 714
XapI RAATTY 2 cut(s) 303, 877
XmaI CCCGGG 1 cut(s) 246
XmiI GTMKAC 2 cut(s) 837, 1072
XspI CTAG 4 cut(s) 158, 476, 605, 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.