MD10G1043300.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009241
Physical Location & Seq
Reverse (-)
5817994 .. 5823629
5636 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1043300.v1.1.491

Sequence Viewer

Length: 1794 bp
ATGTCGATGCTCGATTCCTTCTTCAACAAGGGCTTCAAAGCGGTCAAATGCAAAACCCTGCTGAAATTGACAACTCCGCGGATAAAATTGTTGAGGAACAGGAGAGAAATTCAGATTAAACAGATGCGCCGCGACATTGCCAAGCTTCTTGAGACTGGCCAAGAGGCTACTGCTAGAATTCGGGTGGAGCATATAATTAGAGAAGAGAATATGATGGCAGCTCATGAGATCCTTGAGCTATTTTGCGAGCTTATTGCTGTCCGCCTTCCAATTATTGAATCACAAAGGGAATGCCCTATAGACTTGAAAGAAGCAATATCCAGTGTGTGTTTTGCTGCGCCAAGGTGTGCTGATCTGCCGGAGTTGCTGCAAGTTCAAATGCTATTTGCTGCCAAATATGGGAAAGAATTTGTAGCAGCTGCAACAGAGCTTATACCGGATTGTGGTGTCAATCGCCAGTTGATAGAACTGCTATCTGTTCGTCCTCCCTCACCTGAGAAAAAACTGAAGCTTCTTAAAGAAATTGCTGAAGAGCATGGCCTGGATTGGGATCCTGCTGCATCTGAAGCTGAAATTTACAAACCGCACGAAGATTTGTTGAGTGGTCCAACACAGTTTGTAAGTGGGTCTAAATTGCCCCTTCCCCAAGAAAAACATGATGAAACATTGCAATATGCCCCGGATCAAGCCCAGAAAGAACCGTCTGATTCTGATGACAGTTTGGACTCATTAGACTTTCCTGAAGTTCCTAAGCAATCACTAGGACCAAGTACAAATCCAGCCTCAGCACCAATGATGCCTCCTCCTTTACCAACCCATCCACACCCTGAAATTGATCAAGACTTGTCAAAAAAATGTGGGTCCATTGAAAATCAATCTGAAGAGTCATCCTTTGACTCCGGAGAAGTGATGGAAGAAAGATCAGTAGCTAACAATCAGGAACAGTCTCATCTCTCGGTTGGCGGTGTGGAAGATAAGCAGTTTTTGCCATTTATTTCTGCCCCTTCACTTTCTTCTGTGTCATTTCCTACAAGACAAAGCAATCCACCACCCTCTCTATCAAGAACAAGAAGCGAGGTTAATGTCGATTTGCAGGATGTGTTAGCTGCTGCTCAGGCTGCTGCGGACTCTGCAGAACGTGCAGCGACAGCTGCTCGCTCAGCAGCTAGTCTTGCACAGGTCAGAATCAACGAGATTACCAAGAAAAACAGCGAAGTAGTCCCTAAAAATTGGTCTGAAAGTGAAAACCCTTTTCATGTAGACATTCCTGATCAGTCGGCTACTCAAGCTGCTGCGGACTCTGCAGAACGTGCAGCGACAGCTGCTCCCTCAGCAGCTAGTCTTGCACAGGTTAAAATCAACGAGATTACCAAGAAAAACAGCGAAGAAGCCTCTGAAAATAGGTCTGAAAGTGAAAACCCATTTCATGAAGACGTTCCTAATCAGTCGGATGCTAGAGAAAAACCAGACTTCGATCATCATATCCCCAGTGGTGGTTCTGATGGTGTTATGAATTCTCCGGTACCCCATCAAATCTATTTATATGGCCAGGGATCCGAGATATCAAATGCTTCGTCCATCATTATGGAACCTCTTAATGCCAGTTTTGATTCCCCGCTTCCCAAAGATCATGCTTATGAACACGAGCCTGTTCGTCATCAGCCTCAGAGATTGTCTTCAATGGATGATGACCCATACTTCTCGTACCCAAACTTATTTACATCACAAAATTCAAATGTCGGATCTCATGCTCAGTCTGCTACAGATAATCCGTTCCCTTCCCAAGGACGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

598

Amino Acids

65.67

Weight (kDa)

4.94

Isoelectric Point (pI)

61.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 6.3e-62 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1522
AccB1I GGYRCC 1 cut(s) 1522
AccI GTMKAC 1 cut(s) 1260
AccII CGCG 2 cut(s) 79, 132
AccIII TCCGGA 1 cut(s) 899
AclWI GGATC 7 cut(s) 223, 545, 558, 690, 1548, 1561, 1750
AcoI YGGCCR 2 cut(s) 157, 1546
AcsI RAATTY 6 cut(s) 108, 177, 407, 573, 1513, 1729
AcuI CTGAAG 5 cut(s) 527, 549, 585, 762, 900
AfaI GTAC 3 cut(s) 772, 1524, 1706
AfiI CCNNNNNNNGG 5 cut(s) 399, 443, 547, 1493, 1784
AgsI TTSAA 8 cut(s) 25, 37, 278, 307, 377, 869, 1680, 1734
AjnI CCWGG 2 cut(s) 540, 1548
Alw26I GTCTC 2 cut(s) 146, 951
AlwI GGATC 7 cut(s) 223, 545, 558, 690, 1548, 1561, 1750
Aor13HI TCCGGA 1 cut(s) 899
AoxI GGCC 3 cut(s) 157, 538, 1546
ApoI RAATTY 6 cut(s) 108, 177, 407, 573, 1513, 1729
ArsI GACNNNNNNTTYG 2 cut(s) 1559, 1591
Asp700I GAANNNNTTC 1 cut(s) 1434
Asp718I GGTACC 1 cut(s) 1522
AspLEI GCGC 2 cut(s) 129, 340
AspS9I GGNCC 3 cut(s) 605, 764, 861
AsuC2I CCSGG 1 cut(s) 680
AsuHPI GGTGA 1 cut(s) 483
AvaII GGWCC 3 cut(s) 605, 764, 861
BalI TGGCCA 2 cut(s) 159, 1548
BamHI GGATCC 2 cut(s) 550, 1553
BanI GGYRCC 1 cut(s) 1522
BauI CACGAG 1 cut(s) 1643
BbsI GAAGAC 2 cut(s) 1437, 1668
BbvCI CCTCAGC 2 cut(s) 784, 1330
BccI CCATC 6 cut(s) 208, 825, 904, 1496, 1536, 1586
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 2 cut(s) 542, 1550
BclI TGATCA 2 cut(s) 835, 1270
BcnI CCSGG 1 cut(s) 680
BcoDI GTCTC 2 cut(s) 146, 951
BfaI CTAG 5 cut(s) 174, 761, 1167, 1338, 1455
BfmI CTRYAG 4 cut(s) 297, 1131, 1302, 1761
BlpI GCTNAGC 1 cut(s) 1159
Bme1390I CCNGG 3 cut(s) 542, 680, 1550
Bme18I GGWCC 3 cut(s) 605, 764, 861
BmgT120I GGNCC 3 cut(s) 605, 764, 861
BmiI GGNNCC 5 cut(s) 552, 862, 1524, 1555, 1590
BmrFI CCNGG 3 cut(s) 542, 680, 1550
BmrI ACTGGG 1 cut(s) 1482
BmsI GCATC 4 cut(s) 114, 569, 786, 1441
BmuI ACTGGG 1 cut(s) 1482
BpiI GAAGAC 2 cut(s) 1437, 1668
Bpu10I CCTNAGC 4 cut(s) 750, 784, 1113, 1330
Bpu1102I GCTNAGC 1 cut(s) 1159
BpuEI CTTGAG 3 cut(s) 170, 254, 1269
BpuMI CCSGG 1 cut(s) 680
BsaBI GATNNNNATC 1 cut(s) 549
BsaJI CCNNGG 5 cut(s) 77, 341, 678, 1549, 1783
BsaWI WCCGGW 3 cut(s) 436, 899, 1519
BsaXI ACNNNNNCTCC 2 cut(s) 1309, 1339
Bsc4I CCNNNNNNNGG 5 cut(s) 399, 443, 547, 1493, 1784
Bse1I ACTGG 5 cut(s) 160, 321, 457, 1488, 1602
Bse3DI GCAATG 2 cut(s) 135, 665
Bse8I GATNNNNATC 1 cut(s) 549
BseAI TCCGGA 1 cut(s) 899
BseBI CCWGG 2 cut(s) 542, 1550
BseDI CCNNGG 5 cut(s) 77, 341, 678, 1549, 1783
BseGI GGATG 5 cut(s) 817, 887, 1102, 1456, 1690
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 5 cut(s) 399, 443, 547, 1493, 1784
BseMI GCAATG 2 cut(s) 135, 665
BseMII CTCAG 7 cut(s) 486, 798, 1127, 1173, 1344, 1679, 1766
BseNI ACTGG 5 cut(s) 160, 321, 457, 1488, 1602
BseRI GAGGAG 1 cut(s) 792
BsgI GTGCAG 2 cut(s) 1161, 1332
Bsh1236I CGCG 2 cut(s) 79, 132
BshFI GGCC 3 cut(s) 159, 540, 1548
BshNI GGYRCC 1 cut(s) 1522
BsiSI CCGG 5 cut(s) 359, 437, 680, 900, 1520
BslFI GGGAC 1 cut(s) 1205
BslI CCNNNNNNNGG 5 cut(s) 399, 443, 547, 1493, 1784
BsmAI GTCTC 2 cut(s) 146, 951
BsmFI GGGAC 1 cut(s) 1205
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 3 cut(s) 159, 540, 1548
Bsp13I TCCGGA 1 cut(s) 899
Bsp1720I GCTNAGC 1 cut(s) 1159
BspANI GGCC 3 cut(s) 159, 540, 1548
BspCNI CTCAG 7 cut(s) 487, 797, 1126, 1172, 1343, 1678, 1765
BspEI TCCGGA 1 cut(s) 899
BspFNI CGCG 2 cut(s) 79, 132
BspHI TCATGA 2 cut(s) 223, 1426
BspLI GGNNCC 5 cut(s) 552, 862, 1524, 1555, 1590
BspMAI CTGCAG 2 cut(s) 1135, 1306
BspPI GGATC 7 cut(s) 223, 545, 558, 690, 1548, 1561, 1750
BspQI GCTCTTC 1 cut(s) 525
BspT107I GGYRCC 1 cut(s) 1522
BsrDI GCAATG 2 cut(s) 135, 665
BsrI ACTGG 5 cut(s) 160, 321, 457, 1488, 1602
BssECI CCNNGG 5 cut(s) 77, 341, 678, 1549, 1783
BssSI CACGAG 1 cut(s) 1643
BssT1I CCWWGG 2 cut(s) 341, 1783
Bst2BI CACGAG 1 cut(s) 1643
Bst2UI CCWGG 2 cut(s) 542, 1550
Bst4CI ACNGT 4 cut(s) 615, 702, 719, 945
Bst6I CTCTTC 3 cut(s) 198, 525, 876
BstAPI GCANNNNNTGC 3 cut(s) 985, 1139, 1310
BstC8I GCNNGC 2 cut(s) 248, 1156
BstDEI CTNAG 8 cut(s) 495, 750, 784, 1113, 1159, 1330, 1665, 1752
BstDSI CCRYGG 1 cut(s) 77
BstENI CCTNNNNNAGG 1 cut(s) 1782
BstF5I GGATG 5 cut(s) 817, 887, 1102, 1456, 1690
BstFNI CGCG 2 cut(s) 79, 132
BstHHI GCGC 2 cut(s) 129, 340
BstMAI GTCTC 2 cut(s) 146, 951
BstNI CCWGG 2 cut(s) 542, 1550
BstSCI CCNGG 3 cut(s) 540, 678, 1548
BstSFI CTRYAG 4 cut(s) 297, 1131, 1302, 1761
BstUI CGCG 2 cut(s) 79, 132
BstV2I GAAGAC 2 cut(s) 1437, 1668
BstX2I RGATCY 4 cut(s) 228, 550, 1553, 1742
BstXI CCANNNNNNTGG 1 cut(s) 1585
BstYI RGATCY 4 cut(s) 228, 550, 1553, 1742
BsuRI GGCC 3 cut(s) 159, 540, 1548
BtgI CCRYGG 1 cut(s) 77
BtsCI GGATG 5 cut(s) 817, 887, 1102, 1456, 1690
BtsIMutI CAGTG 2 cut(s) 328, 1495
Cac8I GCNNGC 2 cut(s) 248, 1156
CciI TCATGA 2 cut(s) 223, 1426
CfoI GCGC 2 cut(s) 129, 340
Cfr13I GGNCC 3 cut(s) 605, 764, 861
Cfr42I CCGCGG 1 cut(s) 80
Csp6I GTAC 3 cut(s) 771, 1523, 1705
CviAII CATG 7 cut(s) 224, 536, 656, 1256, 1427, 1631, 1748
CviQI GTAC 3 cut(s) 771, 1523, 1705
DdeI CTNAG 8 cut(s) 495, 750, 784, 1113, 1159, 1330, 1665, 1752
EaeI YGGCCR 2 cut(s) 157, 1546
Eam1104I CTCTTC 3 cut(s) 198, 525, 876
EarI CTCTTC 3 cut(s) 198, 525, 876
EciI GGCGGA 1 cut(s) 251
Eco130I CCWWGG 2 cut(s) 341, 1783
Eco32I GATATC 1 cut(s) 1563
Eco47I GGWCC 3 cut(s) 605, 764, 861
Eco57I CTGAAG 5 cut(s) 527, 549, 585, 762, 900
EcoNI CCTNNNNNAGG 1 cut(s) 1782
EcoRI GAATTC 2 cut(s) 177, 1513
EcoRII CCWGG 2 cut(s) 540, 1548
EcoRV GATATC 1 cut(s) 1563
EcoT14I CCWWGG 2 cut(s) 341, 1783
ErhI CCWWGG 2 cut(s) 341, 1783
FaeI CATG 7 cut(s) 227, 539, 659, 1259, 1430, 1634, 1751
FaqI GGGAC 1 cut(s) 1205
FatI CATG 7 cut(s) 223, 535, 655, 1255, 1426, 1630, 1747
FauI CCCGC 1 cut(s) 1623
FbaI TGATCA 2 cut(s) 835, 1270
FblI GTMKAC 1 cut(s) 1260
FokI GGATG 5 cut(s) 804, 874, 1109, 1463, 1697
FspBI CTAG 5 cut(s) 174, 761, 1167, 1338, 1455
GlaI GCGC 2 cut(s) 128, 339
HaeIII GGCC 3 cut(s) 159, 540, 1548
HapII CCGG 5 cut(s) 359, 437, 680, 900, 1520
HhaI GCGC 2 cut(s) 129, 340
Hin1II CATG 7 cut(s) 227, 539, 659, 1259, 1430, 1634, 1751
Hin6I GCGC 2 cut(s) 127, 338
HinP1I GCGC 2 cut(s) 127, 338
HindIII AAGCTT 2 cut(s) 143, 509
HpaII CCGG 5 cut(s) 359, 437, 680, 900, 1520
HphI GGTGA 1 cut(s) 483
Hpy166II GTNNAC 1 cut(s) 1261
Hpy188III TCNNGA 9 cut(s) 149, 224, 740, 839, 900, 938, 1062, 1268, 1427
Hpy8I GTNNAC 1 cut(s) 1261
HpyAV CCTTC 5 cut(s) 28, 275, 650, 1014, 1788
HpyCH4III ACNGT 4 cut(s) 615, 702, 719, 945
HpyCH4IV ACGT 3 cut(s) 1138, 1309, 1434
HpyF3I CTNAG 8 cut(s) 495, 750, 784, 1113, 1159, 1330, 1665, 1752
HpySE526I ACGT 3 cut(s) 1138, 1309, 1434
Hsp92II CATG 7 cut(s) 227, 539, 659, 1259, 1430, 1634, 1751
HspAI GCGC 2 cut(s) 127, 338
Kpn2I TCCGGA 1 cut(s) 899
KpnI GGTACC 1 cut(s) 1526
Ksp22I TGATCA 2 cut(s) 835, 1270
KspI CCGCGG 1 cut(s) 80
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 2 cut(s) 187, 1330
LweI GCATC 4 cut(s) 114, 569, 786, 1441
MaeI CTAG 5 cut(s) 174, 761, 1167, 1338, 1455
MaeII ACGT 3 cut(s) 1138, 1309, 1434
MflI RGATCY 4 cut(s) 228, 550, 1553, 1742
MlsI TGGCCA 2 cut(s) 159, 1548
MluNI TGGCCA 2 cut(s) 159, 1548
MlyI GAGTC 5 cut(s) 719, 890, 893, 1121, 1292
MmeI TCCRAC 3 cut(s) 632, 1428, 1720
Mox20I TGGCCA 2 cut(s) 159, 1548
MroI TCCGGA 1 cut(s) 899
MroXI GAANNNNTTC 1 cut(s) 1434
MscI TGGCCA 2 cut(s) 159, 1548
MseI TTAA 5 cut(s) 117, 516, 1080, 1353, 1596
MslI CAYNNNNRTG 2 cut(s) 1583, 1635
Msp20I TGGCCA 2 cut(s) 159, 1548
MspA1I CMGCKG 4 cut(s) 79, 419, 1151, 1322
MspI CCGG 5 cut(s) 359, 437, 680, 900, 1520
MspR9I CCNGG 3 cut(s) 542, 680, 1550
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 2 cut(s) 542, 1550
MvnI CGCG 2 cut(s) 79, 132
NciI CCSGG 1 cut(s) 680
NlaIII CATG 7 cut(s) 227, 539, 659, 1259, 1430, 1634, 1751
NlaIV GGNNCC 5 cut(s) 552, 862, 1524, 1555, 1590
PagI TCATGA 2 cut(s) 223, 1426
PciSI GCTCTTC 1 cut(s) 525
PctI GAATGC 1 cut(s) 296
PdmI GAANNNNTTC 1 cut(s) 1434
PfeI GAWTC 5 cut(s) 14, 278, 707, 1185, 1610
PleI GAGTC 5 cut(s) 719, 890, 892, 1121, 1292
PpsI GAGTC 5 cut(s) 719, 890, 892, 1121, 1292
Psp6I CCWGG 2 cut(s) 540, 1548
PspGI CCWGG 2 cut(s) 540, 1548
PspN4I GGNNCC 5 cut(s) 552, 862, 1524, 1555, 1590
PspPI GGNCC 3 cut(s) 605, 764, 861
PstI CTGCAG 2 cut(s) 1135, 1306
PsuI RGATCY 4 cut(s) 228, 550, 1553, 1742
PvuII CAGCTG 3 cut(s) 419, 1151, 1322
RsaI GTAC 3 cut(s) 772, 1524, 1706
RsaNI GTAC 3 cut(s) 771, 1523, 1705
RseI CAYNNNNRTG 2 cut(s) 1583, 1635
SacII CCGCGG 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 5 cut(s) 117, 516, 1080, 1353, 1596
Sau96I GGNCC 3 cut(s) 605, 764, 861
SchI GAGTC 5 cut(s) 719, 890, 893, 1121, 1292
ScrFI CCNGG 3 cut(s) 542, 680, 1550
SfaNI GCATC 4 cut(s) 114, 569, 786, 1441
SfcI CTRYAG 4 cut(s) 297, 1131, 1302, 1761
Sfr303I CCGCGG 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 80
SinI GGWCC 3 cut(s) 605, 764, 861
SmiMI CAYNNNNRTG 2 cut(s) 1583, 1635
SmlI CTYRAG 3 cut(s) 149, 233, 1284
SmoI CTYRAG 3 cut(s) 149, 233, 1284
SspMI CTAG 5 cut(s) 174, 761, 1167, 1338, 1455
StyD4I CCNGG 3 cut(s) 540, 678, 1548
StyI CCWWGG 2 cut(s) 341, 1783
TaaI ACNGT 4 cut(s) 615, 702, 719, 945
TaiI ACGT 3 cut(s) 1141, 1312, 1437
TaqI TCGA 4 cut(s) 5, 12, 1086, 1473
TatI WGTACW 1 cut(s) 770
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 5 cut(s) 14, 278, 707, 1185, 1610
Tru1I TTAA 5 cut(s) 117, 516, 1080, 1353, 1596
Tru9I TTAA 5 cut(s) 117, 516, 1080, 1353, 1596
TscAI CASTG 2 cut(s) 328, 1495
TspDTI ATGAA 6 cut(s) 675, 1244, 1415, 1443, 1526, 1653
TspGWI ACGGA 1 cut(s) 1761
TspRI CASTG 2 cut(s) 328, 1495
VpaK11BI GGWCC 3 cut(s) 605, 764, 861
XagI CCTNNNNNAGG 1 cut(s) 1782
XapI RAATTY 6 cut(s) 108, 177, 407, 573, 1513, 1729
XmiI GTMKAC 1 cut(s) 1260
XmnI GAANNNNTTC 1 cut(s) 1434
XspI CTAG 5 cut(s) 174, 761, 1167, 1338, 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.