Rh6BG190500

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009241
Physical Location & Seq
Forward (+)
35409367 .. 35415183
5817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG190500.1

Sequence Viewer

Length: 1455 bp
ATGCTATTTGTATCTAAATATGGGAAAGAATTTGTATCAGCTGCAACAGAGCTAATGCCAGATTGCGGTGTCAGTCGCCAGTTGATAGAACTGCTATCTGTTCGGGCTCCTTCAGCTGAGAAAAAGCTGAAGCTTTTGAAAGAAATTGCTGAAGAGCATGAACTGGATTGGGATCCTGCTGCATCTGAAGCTGAAATTTTCAAACCGCATGAGGATTTGCTGAATGGTCCGACACAGTTTGTCAGCGGGTCTAAGTTGCCGCTTCCCGAAGAAAAACATGATGAGACGTTGTACCGTGCACCTGATCAAGCTCAGAAAGAACAGTCTGATTCTGATGATGGTTTGGACGTTTTAGACTTTCCGGAAGTTCCGAAGGTATCATCAAGACCAACTGAGAGTATACCCTCAGCACCAGCAATGGCTCCACCTTTTCCACCCCCACCACACCCTGATTTTGATCACGAGTTATCAAAAAATTCTGAGGCGATTGGACATCCATCCAAAGACCCGTCCTTTGAGTCTGGAGAAGAAAGAGCAATGGCAGACAAGCATGAATCTCATGCTTCAGTTGGTCATGGGGATGATGTACAGTTTTTGCCATTTATATCTCCTCCATCTCTTTCATCTGCATCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGGAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTCAGCTGCTGCTCAGGCTGCTGCAGATTCTGCAGCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGGAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTCAGCTGCTGCTCAGGCTGCTGCAGATTCTGCAGCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTTAGCTGCTGCTCAGGCTGCTGCAGATTCGGCAGAACGTGCGGCAGCAGCAGCTCGCTCAGCAGCTAGTCTTGCACAGGTAAGAATAAATGAGCTGACCAAGAAAAACAATGAAGAAGTATCTGAAAGTAGCTGTGAAAACCCATTTCATGAAGACGTTCCTAATCAGTCAGCTACTAAAGAAAAGTCGGATTTTGATCATCATATCCCCGATGGTGATTCTAGTGGTGTTATAAATTCTCCAGAACCCCATCAAGGCCATGAATATGGCCGGGGATCAGAGACATCAAATCTTTCGTTTAAGGACTCGGCTAAAGTGGATTTTGATTCCCCGCTTGCCAGCGATAATGTTTTTGAACATGAGCCAGCTCATCACCAGCCTCAGAGATTGACTTCAATGGATGATGACCCATACTTCTCGTACCCAAACTTATTCACATCAGAAAACAATGTTGGATCTGGTGCTCAGTCTTCTACAGATAATTCTCGCTTCTCGCATGAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

484

Amino Acids

52.47

Weight (kDa)

4.78

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 2 - 55 4.9e-11 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1181
AccI GTMKAC 1 cut(s) 400
AccIII TCCGGA 1 cut(s) 361
AciI CCGC 6 cut(s) 66, 206, 246, 260, 989, 1280
AclWI GGATC 4 cut(s) 167, 180, 1231, 1411
AcoI YGGCCR 1 cut(s) 1216
AcsI RAATTY 4 cut(s) 29, 195, 475, 1183
AcuI CTGAAG 5 cut(s) 96, 149, 171, 207, 549
AfaI GTAC 3 cut(s) 293, 588, 1370
AfiI CCNNNNNNNGG 2 cut(s) 65, 1202
AgsI TTSAA 7 cut(s) 139, 202, 675, 792, 909, 1304, 1344
AjuI GAANNNNNNNTTGG 2 cut(s) 1383, 1415
Alw21I GWGCWC 2 cut(s) 301, 1414
Alw26I GTCTC 5 cut(s) 278, 637, 754, 871, 1223
Alw44I GTGCAC 1 cut(s) 297
AlwI GGATC 4 cut(s) 167, 180, 1231, 1411
AlwNI CAGNNNCTG 4 cut(s) 722, 743, 839, 860
Aor13HI TCCGGA 1 cut(s) 361
AoxI GGCC 2 cut(s) 1204, 1216
ApaLI GTGCAC 1 cut(s) 297
ApoI RAATTY 4 cut(s) 29, 195, 475, 1183
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
Asp700I GAANNNNTTC 1 cut(s) 1104
AspS9I GGNCC 1 cut(s) 227
AsuC2I CCSGG 1 cut(s) 1220
AsuHPI GGTGA 2 cut(s) 1175, 1313
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 1 cut(s) 301
BamHI GGATCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 109
BauI CACGAG 1 cut(s) 461
BbsI GAAGAC 2 cut(s) 1107, 1410
Bbv12I GWGCWC 2 cut(s) 301, 1414
BbvCI CCTCAGC 1 cut(s) 406
BccI CCATC 5 cut(s) 332, 505, 622, 1154, 1206
BclI TGATCA 3 cut(s) 304, 457, 1144
BcnI CCSGG 1 cut(s) 1220
BcoDI GTCTC 5 cut(s) 278, 637, 754, 871, 1223
BfaI CTAG 2 cut(s) 1014, 1170
BfmI CTRYAG 6 cut(s) 735, 744, 852, 861, 969, 1422
BlpI GCTNAGC 1 cut(s) 1006
Bme1390I CCNGG 1 cut(s) 1220
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 3 cut(s) 108, 174, 423
BmrFI CCNGG 1 cut(s) 1220
BmsI GCATC 2 cut(s) 191, 638
BpiI GAAGAC 2 cut(s) 1107, 1410
BpmI CTGGAG 2 cut(s) 543, 1173
Bpu10I CCTNAGC 4 cut(s) 406, 726, 843, 960
Bpu1102I GCTNAGC 1 cut(s) 1006
BpuMI CCSGG 1 cut(s) 1220
BsaBI GATNNNNATC 3 cut(s) 171, 456, 1143
BsaJI CCNNGG 1 cut(s) 1219
BsaWI WCCGGW 1 cut(s) 361
BsaXI ACNNNNNCTCC 4 cut(s) 670, 700, 787, 817
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 1202
Bse1I ACTGG 2 cut(s) 79, 168
Bse3DI GCAATG 2 cut(s) 423, 543
Bse8I GATNNNNATC 3 cut(s) 171, 456, 1143
BseAI TCCGGA 1 cut(s) 361
BseDI CCNNGG 1 cut(s) 1219
BseGI GGATG 4 cut(s) 493, 497, 586, 1354
BseJI GATNNNNATC 3 cut(s) 171, 456, 1143
BseLI CCNNNNNNNGG 2 cut(s) 65, 1202
BseMI GCAATG 2 cut(s) 423, 543
BseNI ACTGG 2 cut(s) 79, 168
BseRI GAGGAG 1 cut(s) 600
BseSI GKGCMC 1 cut(s) 301
BshFI GGCC 2 cut(s) 1206, 1218
BsiHKAI GWGCWC 2 cut(s) 301, 1414
BsiSI CCGG 2 cut(s) 362, 1219
BslI CCNNNNNNNGG 2 cut(s) 65, 1202
BsmAI GTCTC 5 cut(s) 278, 637, 754, 871, 1223
BsmBI CGTCTC 1 cut(s) 278
BsnI GGCC 2 cut(s) 1206, 1218
Bsp1286I GDGCHC 3 cut(s) 109, 301, 1414
Bsp13I TCCGGA 1 cut(s) 361
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 6 cut(s) 172, 304, 457, 1144, 1223, 1403
Bsp1720I GCTNAGC 1 cut(s) 1006
BspACI CCGC 6 cut(s) 66, 206, 246, 260, 989, 1280
BspANI GGCC 2 cut(s) 1206, 1218
BspEI TCCGGA 1 cut(s) 361
BspHI TCATGA 1 cut(s) 1096
BspLI GGNNCC 3 cut(s) 108, 174, 423
BspMAI CTGCAG 5 cut(s) 739, 748, 856, 865, 973
BspPI GGATC 4 cut(s) 167, 180, 1231, 1411
BspQI GCTCTTC 1 cut(s) 147
BsrDI GCAATG 2 cut(s) 423, 543
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 2 cut(s) 79, 168
BssECI CCNNGG 1 cut(s) 1219
BssMI GATC 6 cut(s) 172, 304, 457, 1144, 1223, 1403
BssNAI GTATAC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 461
Bst1107I GTATAC 1 cut(s) 401
Bst2BI CACGAG 1 cut(s) 461
Bst4CI ACNGT 4 cut(s) 237, 296, 324, 591
Bst6I CTCTTC 1 cut(s) 147
BstAPI GCANNNNNTGC 3 cut(s) 743, 860, 986
BstAUI TGTACA 1 cut(s) 586
BstC8I GCNNGC 4 cut(s) 1003, 1284, 1288, 1314
BstF5I GGATG 4 cut(s) 493, 497, 586, 1354
BstKTI GATC 6 cut(s) 175, 307, 460, 1147, 1226, 1406
BstMAI GTCTC 5 cut(s) 278, 637, 754, 871, 1223
BstMBI GATC 6 cut(s) 172, 304, 457, 1144, 1223, 1403
BstSCI CCNGG 1 cut(s) 1218
BstSFI CTRYAG 6 cut(s) 735, 744, 852, 861, 969, 1422
BstSLI GKGCMC 1 cut(s) 301
BstV2I GAAGAC 2 cut(s) 1107, 1410
BstX2I RGATCY 2 cut(s) 172, 1403
BstXI CCANNNNNNTGG 1 cut(s) 1214
BstYI RGATCY 2 cut(s) 172, 1403
BstZ17I GTATAC 1 cut(s) 401
BsuRI GGCC 2 cut(s) 1206, 1218
BtsCI GGATG 4 cut(s) 493, 497, 586, 1354
BtsIMutI CAGTG 1 cut(s) 1450
Cac8I GCNNGC 4 cut(s) 1003, 1284, 1288, 1314
CaiI CAGNNNCTG 4 cut(s) 722, 743, 839, 860
CciI TCATGA 1 cut(s) 1096
Cfr13I GGNCC 1 cut(s) 227
Csp6I GTAC 3 cut(s) 292, 587, 1369
CviQI GTAC 3 cut(s) 292, 587, 1369
DpnI GATC 6 cut(s) 174, 306, 459, 1146, 1225, 1405
DpnII GATC 6 cut(s) 172, 304, 457, 1144, 1223, 1403
EaeI YGGCCR 1 cut(s) 1216
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 5 cut(s) 96, 149, 171, 207, 549
EcoT38I GRGCYC 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 278
FauI CCCGC 2 cut(s) 239, 1287
FbaI TGATCA 3 cut(s) 304, 457, 1144
FblI GTMKAC 1 cut(s) 400
FokI GGATG 4 cut(s) 480, 484, 593, 1361
FriOI GRGCYC 1 cut(s) 109
FspBI CTAG 2 cut(s) 1014, 1170
GsuI CTGGAG 2 cut(s) 543, 1173
HaeIII GGCC 2 cut(s) 1206, 1218
HapII CCGG 2 cut(s) 362, 1219
HindIII AAGCTT 1 cut(s) 131
HpaII CCGG 2 cut(s) 362, 1219
HphI GGTGA 2 cut(s) 1175, 1313
Hpy166II GTNNAC 2 cut(s) 299, 401
Hpy188III TCNNGA 8 cut(s) 266, 362, 384, 461, 522, 909, 1097, 1190
Hpy8I GTNNAC 2 cut(s) 299, 401
HpyAV CCTTC 2 cut(s) 120, 367
HpyCH4III ACNGT 4 cut(s) 237, 296, 324, 591
HpyCH4IV ACGT 4 cut(s) 287, 348, 985, 1104
HpySE526I ACGT 4 cut(s) 287, 348, 985, 1104
Kpn2I TCCGGA 1 cut(s) 361
Ksp22I TGATCA 3 cut(s) 304, 457, 1144
Kzo9I GATC 6 cut(s) 172, 304, 457, 1144, 1223, 1403
LguI GCTCTTC 1 cut(s) 147
LmnI GCTCC 2 cut(s) 112, 427
LweI GCATC 2 cut(s) 191, 638
MaeI CTAG 2 cut(s) 1014, 1170
MaeII ACGT 4 cut(s) 287, 348, 985, 1104
MalI GATC 6 cut(s) 174, 306, 459, 1146, 1225, 1405
MboI GATC 6 cut(s) 172, 304, 457, 1144, 1223, 1403
MboII GAAGA 7 cut(s) 164, 281, 539, 924, 1073, 1112, 1410
MflI RGATCY 2 cut(s) 172, 1403
MhlI GDGCHC 3 cut(s) 109, 301, 1414
MluCI AATT 6 cut(s) 29, 144, 195, 475, 1183, 1429
MlyI GAGTC 2 cut(s) 527, 1247
MmeI TCCRAC 3 cut(s) 254, 1116, 1381
MroI TCCGGA 1 cut(s) 361
MroXI GAANNNNTTC 1 cut(s) 1104
MseI TTAA 1 cut(s) 1248
MslI CAYNNNNRTG 2 cut(s) 579, 1212
MspA1I CMGCKG 5 cut(s) 41, 116, 246, 719, 836
MspI CCGG 2 cut(s) 362, 1219
MspR9I CCNGG 1 cut(s) 1220
NciI CCSGG 1 cut(s) 1220
NdeII GATC 6 cut(s) 172, 304, 457, 1144, 1223, 1403
NlaIV GGNNCC 3 cut(s) 108, 174, 423
NmeAIII GCCGAG 1 cut(s) 1235
PagI TCATGA 1 cut(s) 1096
PciSI GCTCTTC 1 cut(s) 147
PdmI GAANNNNTTC 1 cut(s) 1104
PleI GAGTC 2 cut(s) 526, 1247
PpsI GAGTC 2 cut(s) 526, 1247
PsiI TTATAA 1 cut(s) 1181
PspN4I GGNNCC 3 cut(s) 108, 174, 423
PspPI GGNCC 1 cut(s) 227
PstI CTGCAG 5 cut(s) 739, 748, 856, 865, 973
PstNI CAGNNNCTG 4 cut(s) 722, 743, 839, 860
PsuI RGATCY 2 cut(s) 172, 1403
PvuII CAGCTG 4 cut(s) 41, 116, 719, 836
RsaI GTAC 3 cut(s) 293, 588, 1370
RsaNI GTAC 3 cut(s) 292, 587, 1369
RseI CAYNNNNRTG 2 cut(s) 579, 1212
SapI GCTCTTC 1 cut(s) 147
SaqAI TTAA 1 cut(s) 1248
Sau3AI GATC 6 cut(s) 172, 304, 457, 1144, 1223, 1403
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 2 cut(s) 527, 1247
ScrFI CCNGG 1 cut(s) 1220
SduI GDGCHC 3 cut(s) 109, 301, 1414
SfaNI GCATC 2 cut(s) 191, 638
SfcI CTRYAG 6 cut(s) 735, 744, 852, 861, 969, 1422
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 2 cut(s) 579, 1212
Sse9I AATT 6 cut(s) 29, 144, 195, 475, 1183, 1429
SsiI CCGC 6 cut(s) 66, 206, 246, 260, 989, 1280
SspMI CTAG 2 cut(s) 1014, 1170
StyD4I CCNGG 1 cut(s) 1218
TaaI ACNGT 4 cut(s) 237, 296, 324, 591
TaiI ACGT 4 cut(s) 290, 351, 988, 1107
TasI AATT 6 cut(s) 29, 144, 195, 475, 1183, 1429
TatI WGTACW 1 cut(s) 586
TauI GCSGC 2 cut(s) 262, 992
Tru1I TTAA 1 cut(s) 1248
Tru9I TTAA 1 cut(s) 1248
TspDTI ATGAA 7 cut(s) 174, 567, 612, 1074, 1085, 1113, 1224
VneI GTGCAC 1 cut(s) 297
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 4 cut(s) 29, 195, 475, 1183
XmiI GTMKAC 1 cut(s) 400
XmnI GAANNNNTTC 1 cut(s) 1104
XspI CTAG 2 cut(s) 1014, 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.