Rh6CG189800

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009241
Physical Location & Seq
Forward (+)
30945489 .. 30950465
4977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG189800.1

Sequence Viewer

Length: 1464 bp
ATGTCGATGCTCGATTCCTTCTTCAACAAGGGCTTCAAAGCTGCCAAATGGGAGTGCCCTCTAGACTTGAAAGAAGCAATATCCAGTGTGTGTTTTGCTGCACCAAGATGCGCTGATCTGCCGGAGTTGCTTCAAGTTCAAATGCTATTTGTATCTAAATATGGGAAAGAATTTGTATCAGCTGCAACAGAGCTAATGCCAGATTGTGGTGTCAGTCGCCAGTTGATAGAGCTGCTATCTGTTCGGGCTCCTTCAGCTGAGAAAAAGCTGAAGCTTTTGAAAGAAATTGCTGAAGAGCATGAACTGGATTGGGATCCTGCTGCATCTGAAGCTGAAATTTTCAAACCGCATGAGGATTTGCTGAATGGTCCGACACAGTTTGTCAGCGGGTCTAAGTTGCCGCTTCCCGAAGAAAAACATGATGAGACGTTGTACCGCGCGCCTGATCAAGCTCAGAAAGAACAGTCTGATTCTGATGATGGTTTGGACGTTTTAGACTTTCCGGAAGTTCCGAAGGTATCATCAAGACCAACTGAGAGTATACCCTCAGCACCAGCAATGGCTCCACCTTTTCCACCCCCACCACACCCTGATTTTGATCACGAGTTATCAAAAAATTCTGAGGCGATTGGACATCCATCCAAAGAGCCGTCCTTTGAGTCTGGAGAAGAAAGAGCAATGGCAGACAAGCATGAATCTCATGCTTCAGTTGGTCATGGGGATGATGTACAGTTTTTGCCATTTATATCTCCTCCATCTCTTTCATCTGCATCGTTTCCTGTGAGACAAACGAATCCACCACCACCCTCTCTTTCAAGAACAAAAAGTGAGGTCAATGTGGATATGCAAGATGTGTCAGCTGCTGCAGATTCTGCAGCGTTTCCTGTGAGAAGAACGAATCCACCACCACCCTCTCTTTCAAGAAGAAAAAGTGAGGTCAATGTAGATATGCAAGATGTGTTAGCTGCTGCTCAGGCTGCTGCAGATTCGGCAGAACGTGCGGCAGCAGCAGCTCGCTCAGCAGCTAGTCTTGCACAGGTAAGAATAAATGAGCTGACCAAGAAAAACAATGACGTATCTGAAAGTAGCTGTGAAAACCCATTTCATGAAGACGTTCCTAATCAGTCAGCTACTAGAGAAAAGTCGGATTTTGATCATCATATCCCGGATGGTGATTCTAGTGGTGTTATAAATTCTCCAGAACCCCATCAAGGCCATGAATATGGCCGGGGATCAGAGACATCAAATCTTTCGTTTAAGGACTCGGCTAAAGTGGATTTTGATTCCCCGCTTCCCAGCGATAATGTTTTTGAACACGAGCCAGCTCATCACCAGCCTCAGAGATTGACTTCAATGGATGATGACCCATACTTCTCGTACCCAAACTTATTCACATCAGAAAACAATGTTGGATCTGGTGCTCAGTCTTCTACAGATAATTCTCGCTTCTCGCATGAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

487

Amino Acids

53.19

Weight (kDa)

4.79

Isoelectric Point (pI)

54.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 17 - 102 2.9e-26 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1190
AccB7I CCANNNNNTGG 1 cut(s) 206
AccI GTMKAC 1 cut(s) 541
AccII CGCG 2 cut(s) 438, 440
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 6 cut(s) 347, 387, 401, 436, 1001, 1289
AclWI GGATC 4 cut(s) 308, 321, 1240, 1420
AcoI YGGCCR 1 cut(s) 1225
AcsI RAATTY 4 cut(s) 170, 336, 616, 1192
AcuI CTGAAG 5 cut(s) 237, 290, 312, 348, 690
AfaI GTAC 3 cut(s) 434, 729, 1379
AfiI CCNNNNNNNGG 2 cut(s) 206, 1211
AjuI GAANNNNNNNTTGG 2 cut(s) 1392, 1424
Alw21I GWGCWC 1 cut(s) 1423
Alw26I GTCTC 3 cut(s) 419, 778, 1232
AlwI GGATC 4 cut(s) 308, 321, 1240, 1420
AlwNI CAGNNNCTG 2 cut(s) 863, 872
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 2 cut(s) 1213, 1225
ApoI RAATTY 4 cut(s) 170, 336, 616, 1192
Asp700I GAANNNNTTC 1 cut(s) 1113
AspLEI GCGC 3 cut(s) 113, 440, 442
AspS9I GGNCC 1 cut(s) 368
AsuC2I CCSGG 2 cut(s) 1166, 1229
AsuHPI GGTGA 2 cut(s) 1184, 1322
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 59
BamHI GGATCC 1 cut(s) 313
BanII GRGCYC 1 cut(s) 250
BauI CACGAG 2 cut(s) 602, 1316
BbsI GAAGAC 2 cut(s) 1116, 1419
Bbv12I GWGCWC 1 cut(s) 1423
BbvCI CCTCAGC 1 cut(s) 547
BccI CCATC 5 cut(s) 473, 646, 763, 1163, 1215
BceAI ACGGC 1 cut(s) 634
BclI TGATCA 3 cut(s) 445, 598, 1153
BcnI CCSGG 2 cut(s) 1166, 1229
BcoDI GTCTC 3 cut(s) 419, 778, 1232
BfaI CTAG 4 cut(s) 62, 1026, 1134, 1179
BfmI CTRYAG 4 cut(s) 864, 873, 981, 1431
BlpI GCTNAGC 1 cut(s) 1018
Bme1390I CCNGG 2 cut(s) 1166, 1229
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 3 cut(s) 249, 315, 564
BmrFI CCNGG 2 cut(s) 1166, 1229
BmsI GCATC 3 cut(s) 98, 332, 779
BpiI GAAGAC 2 cut(s) 1116, 1419
BpmI CTGGAG 2 cut(s) 684, 1182
Bpu10I CCTNAGC 2 cut(s) 547, 972
Bpu1102I GCTNAGC 1 cut(s) 1018
BpuMI CCSGG 2 cut(s) 1166, 1229
BsaBI GATNNNNATC 3 cut(s) 312, 597, 1152
BsaJI CCNNGG 1 cut(s) 1228
BsaWI WCCGGW 1 cut(s) 502
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 1211
Bse1I ACTGG 3 cut(s) 84, 220, 309
Bse3DI GCAATG 2 cut(s) 564, 684
Bse8I GATNNNNATC 3 cut(s) 312, 597, 1152
BseAI TCCGGA 1 cut(s) 502
BseDI CCNNGG 1 cut(s) 1228
BseGI GGATG 5 cut(s) 634, 638, 727, 1174, 1363
BseJI GATNNNNATC 3 cut(s) 312, 597, 1152
BseLI CCNNNNNNNGG 2 cut(s) 206, 1211
BseMI GCAATG 2 cut(s) 564, 684
BseMII CTCAG 9 cut(s) 249, 467, 525, 561, 612, 986, 1032, 1352, 1436
BseNI ACTGG 3 cut(s) 84, 220, 309
BsePI GCGCGC 1 cut(s) 438
BseRI GAGGAG 1 cut(s) 741
BseSI GKGCMC 1 cut(s) 59
BseYI CCCAGC 1 cut(s) 1295
BsgI GTGCAG 1 cut(s) 84
Bsh1236I CGCG 2 cut(s) 438, 440
BshFI GGCC 2 cut(s) 1215, 1227
BsiHKAI GWGCWC 1 cut(s) 1423
BsiSI CCGG 4 cut(s) 122, 503, 1166, 1228
BslI CCNNNNNNNGG 2 cut(s) 206, 1211
BsmAI GTCTC 3 cut(s) 419, 778, 1232
BsmBI CGTCTC 1 cut(s) 419
BsnI GGCC 2 cut(s) 1215, 1227
Bsp1286I GDGCHC 3 cut(s) 59, 250, 1423
Bsp13I TCCGGA 1 cut(s) 502
Bsp1407I TGTACA 1 cut(s) 727
Bsp143I GATC 7 cut(s) 115, 313, 445, 598, 1153, 1232, 1412
Bsp1720I GCTNAGC 1 cut(s) 1018
BspACI CCGC 6 cut(s) 347, 387, 401, 436, 1001, 1289
BspANI GGCC 2 cut(s) 1215, 1227
BspCNI CTCAG 9 cut(s) 250, 466, 526, 560, 613, 985, 1031, 1351, 1435
BspEI TCCGGA 1 cut(s) 502
BspFNI CGCG 2 cut(s) 438, 440
BspHI TCATGA 1 cut(s) 1105
BspLI GGNNCC 3 cut(s) 249, 315, 564
BspMAI CTGCAG 3 cut(s) 868, 877, 985
BspPI GGATC 4 cut(s) 308, 321, 1240, 1420
BspQI GCTCTTC 1 cut(s) 288
BsrDI GCAATG 2 cut(s) 564, 684
BsrGI TGTACA 1 cut(s) 727
BsrI ACTGG 3 cut(s) 84, 220, 309
BssECI CCNNGG 1 cut(s) 1228
BssHII GCGCGC 1 cut(s) 438
BssMI GATC 7 cut(s) 115, 313, 445, 598, 1153, 1232, 1412
BssNAI GTATAC 1 cut(s) 542
BssSI CACGAG 2 cut(s) 602, 1316
Bst1107I GTATAC 1 cut(s) 542
Bst2BI CACGAG 2 cut(s) 602, 1316
Bst4CI ACNGT 3 cut(s) 378, 465, 732
Bst6I CTCTTC 1 cut(s) 288
BstAPI GCANNNNNTGC 2 cut(s) 872, 998
BstAUI TGTACA 1 cut(s) 727
BstC8I GCNNGC 3 cut(s) 440, 1015, 1323
BstF5I GGATG 5 cut(s) 634, 638, 727, 1174, 1363
BstFNI CGCG 2 cut(s) 438, 440
BstHHI GCGC 3 cut(s) 113, 440, 442
BstKTI GATC 7 cut(s) 118, 316, 448, 601, 1156, 1235, 1415
BstMAI GTCTC 3 cut(s) 419, 778, 1232
BstMBI GATC 7 cut(s) 115, 313, 445, 598, 1153, 1232, 1412
BstSCI CCNGG 2 cut(s) 1164, 1227
BstSFI CTRYAG 4 cut(s) 864, 873, 981, 1431
BstSLI GKGCMC 1 cut(s) 59
BstUI CGCG 2 cut(s) 438, 440
BstV2I GAAGAC 2 cut(s) 1116, 1419
BstX2I RGATCY 2 cut(s) 313, 1412
BstXI CCANNNNNNTGG 1 cut(s) 1223
BstYI RGATCY 2 cut(s) 313, 1412
BstZ17I GTATAC 1 cut(s) 542
BsuRI GGCC 2 cut(s) 1215, 1227
BtsCI GGATG 5 cut(s) 634, 638, 727, 1174, 1363
BtsIMutI CAGTG 2 cut(s) 91, 1459
Cac8I GCNNGC 3 cut(s) 440, 1015, 1323
CaiI CAGNNNCTG 2 cut(s) 863, 872
CciI TCATGA 1 cut(s) 1105
CfoI GCGC 3 cut(s) 113, 440, 442
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 3 cut(s) 433, 728, 1378
CviAII CATG 9 cut(s) 299, 350, 419, 692, 701, 716, 1106, 1217, 1454
CviQI GTAC 3 cut(s) 433, 728, 1378
DpnI GATC 7 cut(s) 117, 315, 447, 600, 1155, 1234, 1414
DpnII GATC 7 cut(s) 115, 313, 445, 598, 1153, 1232, 1412
EaeI YGGCCR 1 cut(s) 1225
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 368
Eco57I CTGAAG 5 cut(s) 237, 290, 312, 348, 690
EcoT38I GRGCYC 1 cut(s) 250
Esp3I CGTCTC 1 cut(s) 419
FaeI CATG 9 cut(s) 302, 353, 422, 695, 704, 719, 1109, 1220, 1457
FatI CATG 9 cut(s) 298, 349, 418, 691, 700, 715, 1105, 1216, 1453
FauI CCCGC 2 cut(s) 380, 1296
FbaI TGATCA 3 cut(s) 445, 598, 1153
FblI GTMKAC 1 cut(s) 541
FokI GGATG 5 cut(s) 621, 625, 734, 1181, 1370
FriOI GRGCYC 1 cut(s) 250
FspBI CTAG 4 cut(s) 62, 1026, 1134, 1179
GlaI GCGC 3 cut(s) 112, 439, 441
GsaI CCCAGC 1 cut(s) 1299
GsuI CTGGAG 2 cut(s) 684, 1182
HaeIII GGCC 2 cut(s) 1215, 1227
HapII CCGG 4 cut(s) 122, 503, 1166, 1228
HhaI GCGC 3 cut(s) 113, 440, 442
Hin1II CATG 9 cut(s) 302, 353, 422, 695, 704, 719, 1109, 1220, 1457
Hin6I GCGC 3 cut(s) 111, 438, 440
HinP1I GCGC 3 cut(s) 111, 438, 440
HindIII AAGCTT 1 cut(s) 272
HpaII CCGG 4 cut(s) 122, 503, 1166, 1228
HphI GGTGA 2 cut(s) 1184, 1322
Hpy166II GTNNAC 1 cut(s) 542
Hpy8I GTNNAC 1 cut(s) 542
HpyAV CCTTC 3 cut(s) 28, 261, 508
HpyCH4III ACNGT 3 cut(s) 378, 465, 732
HpyCH4IV ACGT 5 cut(s) 428, 489, 997, 1074, 1113
HpySE526I ACGT 5 cut(s) 428, 489, 997, 1074, 1113
Hsp92II CATG 9 cut(s) 302, 353, 422, 695, 704, 719, 1109, 1220, 1457
HspAI GCGC 3 cut(s) 111, 438, 440
Kpn2I TCCGGA 1 cut(s) 502
Ksp22I TGATCA 3 cut(s) 445, 598, 1153
Kzo9I GATC 7 cut(s) 115, 313, 445, 598, 1153, 1232, 1412
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 2 cut(s) 253, 568
LweI GCATC 3 cut(s) 98, 332, 779
MaeI CTAG 4 cut(s) 62, 1026, 1134, 1179
MaeII ACGT 5 cut(s) 428, 489, 997, 1074, 1113
MalI GATC 7 cut(s) 117, 315, 447, 600, 1155, 1234, 1414
MboI GATC 7 cut(s) 115, 313, 445, 598, 1153, 1232, 1412
MboII GAAGA 8 cut(s) 13, 305, 422, 680, 903, 936, 1121, 1419
MflI RGATCY 2 cut(s) 313, 1412
MhlI GDGCHC 3 cut(s) 59, 250, 1423
MluCI AATT 6 cut(s) 170, 285, 336, 616, 1192, 1438
MlyI GAGTC 2 cut(s) 668, 1256
MmeI TCCRAC 3 cut(s) 395, 1125, 1390
MroI TCCGGA 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 1113
MseI TTAA 1 cut(s) 1257
MslI CAYNNNNRTG 3 cut(s) 106, 720, 1221
MspA1I CMGCKG 4 cut(s) 182, 257, 387, 860
MspI CCGG 4 cut(s) 122, 503, 1166, 1228
MspR9I CCNGG 2 cut(s) 1166, 1229
MvnI CGCG 2 cut(s) 438, 440
NciI CCSGG 2 cut(s) 1166, 1229
NdeII GATC 7 cut(s) 115, 313, 445, 598, 1153, 1232, 1412
NlaIII CATG 9 cut(s) 302, 353, 422, 695, 704, 719, 1109, 1220, 1457
NlaIV GGNNCC 3 cut(s) 249, 315, 564
NmeAIII GCCGAG 1 cut(s) 1244
PagI TCATGA 1 cut(s) 1105
PauI GCGCGC 1 cut(s) 438
PciSI GCTCTTC 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 1113
PfeI GAWTC 9 cut(s) 14, 470, 695, 793, 869, 898, 986, 1175, 1283
PflMI CCANNNNNTGG 1 cut(s) 206
PfoI TCCNGGA 1 cut(s) 1164
PleI GAGTC 2 cut(s) 667, 1256
PpsI GAGTC 2 cut(s) 667, 1256
PsiI TTATAA 1 cut(s) 1190
PspFI CCCAGC 1 cut(s) 1295
PspN4I GGNNCC 3 cut(s) 249, 315, 564
PspPI GGNCC 1 cut(s) 368
PstI CTGCAG 3 cut(s) 868, 877, 985
PstNI CAGNNNCTG 2 cut(s) 863, 872
PsuI RGATCY 2 cut(s) 313, 1412
PteI GCGCGC 1 cut(s) 438
PvuII CAGCTG 3 cut(s) 182, 257, 860
RsaI GTAC 3 cut(s) 434, 729, 1379
RsaNI GTAC 3 cut(s) 433, 728, 1378
RseI CAYNNNNRTG 3 cut(s) 106, 720, 1221
SapI GCTCTTC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 1257
Sau3AI GATC 7 cut(s) 115, 313, 445, 598, 1153, 1232, 1412
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 2 cut(s) 668, 1256
ScrFI CCNGG 2 cut(s) 1166, 1229
SduI GDGCHC 3 cut(s) 59, 250, 1423
SfaNI GCATC 3 cut(s) 98, 332, 779
SfcI CTRYAG 4 cut(s) 864, 873, 981, 1431
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 3 cut(s) 106, 720, 1221
Sse9I AATT 6 cut(s) 170, 285, 336, 616, 1192, 1438
SsiI CCGC 6 cut(s) 347, 387, 401, 436, 1001, 1289
SspMI CTAG 4 cut(s) 62, 1026, 1134, 1179
StyD4I CCNGG 2 cut(s) 1164, 1227
TaaI ACNGT 3 cut(s) 378, 465, 732
TaiI ACGT 5 cut(s) 431, 492, 1000, 1077, 1116
TaqI TCGA 2 cut(s) 5, 12
TasI AATT 6 cut(s) 170, 285, 336, 616, 1192, 1438
TatI WGTACW 1 cut(s) 727
TauI GCSGC 2 cut(s) 403, 1004
TfiI GAWTC 9 cut(s) 14, 470, 695, 793, 869, 898, 986, 1175, 1283
Tru1I TTAA 1 cut(s) 1257
Tru9I TTAA 1 cut(s) 1257
TscAI CASTG 1 cut(s) 91
TspDTI ATGAA 6 cut(s) 315, 708, 753, 1094, 1122, 1233
TspRI CASTG 1 cut(s) 91
Van91I CCANNNNNTGG 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 4 cut(s) 170, 336, 616, 1192
XbaI TCTAGA 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 541
XmnI GAANNNNTTC 1 cut(s) 1113
XspI CTAG 4 cut(s) 62, 1026, 1134, 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.